Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus

Using previously developed spectro-photometrical method (Bioorg. Khim. 2009. V. 35. pp. 629-639), a significant increase of myeloperoxidase (MPO) activity was found in blood plasma of patients with type 2 diabetes mellitus (DM2) without of cardiovascular complications, as well as with ischemic heart disease (IHD). Plasma MPO concentration measured by an enzyme-linked immunosorbent assay was significantly higher only in blood plasma of patient with DM2 and IHD. A direct and significant correlation between MPO activity and MPO concentration was observed only in blood plasma samples from healthy donors. Increased MPO activity did not correlate with MPO concentration in blood plasma of patients with DM2 and DM2 with IHD. Taken together, these results highlight the necessity for studying of the MPO role in the development of pathological processes to determine both the amount of enzyme and its peroxidase activity in the blood. The proposed approach gives comprehensive information about the relationship between MPO activity and MPO concentration in patient blood. Since the high concentration of MPO is a diagnostically significant parameter in the prediction of endothelial dysfunction and cardiovascular disease development, the obtained results evidence the contribution of MPO-dependent reactions in cardiovascular complications associated with diabetes. MPO activity may serve as an additional diagnostic criterion for determination of risk of IHD in DM patients.

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Effect of Simvastatin on the spectrum of blood plasma fat acids change in the patients with ischemia heart disease

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2008-3-13-19 ◽

2008 ◽

Vol 7 (3) ◽

pp. 13-19

Author(s):

V. V. Novitsky ◽

R. S. Karpov ◽

M. Yu. Kotlovsky ◽

S. V. Klemenkov ◽

O. V. Voronkova ◽

...

Keyword(s):

Heart Disease ◽

Blood Plasma ◽

Total Concentration ◽

Saturated Fat ◽

Constant Level ◽

Simvastatin Treatment ◽

Total Level ◽

Total Percentage ◽

Healthy Donors ◽

In Blood Plasma

The intake of Simvastatin in a dosage of 40 mg daily within two months in patients with ischemia heart disease has caused increase in the general percentage of ω6 fat acids (FA) at a constant level of ω3 FA, that was not accompanied by change of a ratio ω3/ω6 FA. Despite of progressing increase in total concentration of ω7 FA (at women) and increase of a level ω9 FA (at men) during simvastatin treatment at women and at men led to decrease in the total percentage of nonsaturated fat acids concerning corresponding parameters at healthy donors. The Simvastatin treatment was accompanied by increase in blood plasma in patients with ischemia heart disease (and first of all at women) percentage of the separate saturated fat acids, and also their total level. The index of nonsaturation of fat acids of blood plasma is decreased.

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Effects of COVID-19, diabetes mellitus, and cardiovascular diseases on insulin receptor substrate-1 amount in the blood plasma of patients

Reports of the National Academy of Sciences of Ukraine ◽

10.15407/dopovidi2021.05.114 ◽

2021 ◽

pp. 114-117

Author(s):

M. D. Tronko ◽

V. V. Pushkarev ◽

L. K. Sokolova ◽

S. A. Cherviakova ◽

Yu. B. Belchina ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Plasma ◽

Insulin Receptor ◽

Enzyme Linked Immunosorbent Assay ◽

Insulin Receptor Substrate ◽

Adapter Protein ◽

Mediating Effects ◽

Optical Wavelength ◽

Patients With Diabetes ◽

Insulin Like Growth Factors

Insulin receptor substrate (IRS) is a key adapter protein mediating effects of insulin and insulin-like growth factors (IGF) in cells. IRS-1 is a member of the insulin receptor substrate family, which is associated with tumor initiation and progression. The aim of the study is to determine the level of IRS-1 in the blood of patients (n = 81) with diabetes mellitus and COVID-19. IRS-1 was determined with enzyme-linked immunosorbent assay (ELISA) (Elabscience, USA). The measurements were performed at an optical wavelength of 450 nm. The level of IRS-1 in the blood plasma of patients with COVID-19 was much (from 3.5 to more than 6 times) higher than that in the blood of healthy people. The IRS-1 amounts in COVID-19 patients with diabetes and diabetes + CVD were significantly higher than in patients with COVID-19 without concomitant diseases. The level of IRS-1 in blood plasma may be one of the promising markers of COVID-19.

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Lack of association between peroxisome proliferator-activated receptor-gamma-2 gene variants and the occurrence of coronary heart disease in patients with diabetes mellitus

Acta Endocrinologica ◽

10.1530/eje.0.1460545 ◽

2002 ◽

pp. 545-551 ◽

Cited By ~ 23

Author(s):

M Bluher ◽

T Klemm ◽

T Gerike ◽

H Krankenberg ◽

G Schuler ◽

...

Keyword(s):

Diabetes Mellitus ◽

Risk Factors ◽

Coronary Heart Disease ◽

Heart Disease ◽

Multiple Logistic Regression Analysis ◽

Peroxisome Proliferator ◽

Haemoglobin A1c ◽

Peroxisome Proliferator Activated Receptor ◽

Patients With Diabetes ◽

Reduced Risk

OBJECTIVE: Recent evidence indicates that peroxisome proliferator-activated receptor-gamma (PPARgamma) is expressed at high levels in foam cells of atherosclerotic lesions, that PPARgamma agonists may directly modulate vessel wall function and that mutations in the PPARgamma-2 gene are associated with a reduced risk of coronary artery disease. METHODS: We investigated whether known variants in the PPARgamma-2 gene are associated with the occurrence of coronary heart disease (CHD) in 365 patients with type 2 diabetes, prospectively characterised for the presence or absence of CHD. The Pro115Gln, Pro12Ala, Pro467Leu, Val290Met mutations and two polymorphisms C478T and C161T of the PPARgamma-2 gene were examined using PCR, denaturing gradient gel electrophoresis and direct sequencing. RESULTS: The distribution of the Pro12Ala, Ala12Ala, C161T and T161T variants was not significantly different between patients with and without CHD, independent of the gender. The Pro12Ala (P=0.011) and the Ala12Ala (P=0.006) variant were associated with a higher body mass index (BMI) compared with the Pro12Pro genotype. A multiple logistic regression analysis introducing the typical risk factors for CHD (age, sex, hypertension, smoking, BMI >26 kg/m2, elevated low density lipoprotein cholesterol and haemoglobin A1c >7%) identified age >60, male gender, hypertension and a higher BMI, but not the PPARgamma-2 variants, as significant risk factors for CHD in our study groups. CONCLUSION: The PPARgamma-2 genotype was not associated with an increased or reduced risk of the occurrence of CHD and can therefore not be regarded as an independent risk factor for CHD in patients with diabetes mellitus.

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MANAGEMENT AND OUTCOMES OF PATIENTS WITH DIABETES MELLITUS (DM) AND STABLE ISCHEMIC HEART DISEASE (SIHD): POOLED DATA FROM THE ISCHEMIA AND ISCHEMIA-CKD TRIALS

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(21)01497-2 ◽

2021 ◽

Vol 77 (18) ◽

pp. 138

Author(s):

Jonathan Newman ◽

Rebecca Anthopolos ◽

G.B. John Mancini ◽

Sripal Bangalore ◽

Harmony Reynolds ◽

...

Keyword(s):

Diabetes Mellitus ◽

Heart Disease ◽

Ischemic Heart Disease ◽

Ischemic Heart ◽

Pooled Data ◽

Stable Ischemic Heart Disease ◽

Patients With Diabetes

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Immunological aspects of bacterial keratites in patients with diabetes mellitus

Archive of Ukrainian Ophthalmology ◽

10.22141/2309-8147.9.2.2021.241501 ◽

2021 ◽

Vol 9 (2) ◽

pp. 6-9

Author(s):

O.V. Zavoloka ◽

P.A. Bezditko ◽

L.P. Abramova ◽

V.O. Vekshyn

Keyword(s):

Diabetes Mellitus ◽

Anterior Segment ◽

Enzyme Linked Immunosorbent Assay ◽

Bacterial Keratitis ◽

Healthy Individuals ◽

Lacrimal Fluid ◽

Cytokine Balance ◽

Patients With Diabetes ◽

Contralateral Eye ◽

Type 1 Dm

Background. The purpose was to analyze the cytokine balance of lacrimal fluid in patients with bacterial keratitis and diabetes mellitus (DM) at the first visit and to identify the immunological aspects of the disease. Materials and methods. The analysis of pro- and anti-inflammatory cytokine concentration in the lacrimal fluid was performed in 17 patients with type 1 DM and bacterial keratitis and 15 nondiabetic patients with bacterial keratitis at the first visit. Data from 14 healthy individuals were used for comparison. In addition to standard ones, ophthalmic examination methods included bacteriological examination, fluorescein test, anterior segment optical coherence tomography, non-contact corneal aesthesiometry. The levels of interleukin (IL) 1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye were determined by a quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits. Results. In DM patients with bacterial keratitis, the concentration of IL-1β and IL-6 in the lacrimal fluid of the sick eye exceeded that in healthy individuals (p < 0.05) and did not differ significantly from nondiabetic patients with bacterial keratitis (p > 0.05). In the lacrimal fluid of the contralateral eye of DM patients with bacterial keratitis, the level of IL-1β and IL-6 exceeded the corresponding indicators of nondiabetic patients with bacterial keratitis and healthy individuals (p < 0.05). The concentration of IL-10 in the lacrimal fluid of the contralateral eye in DM patients with bacterial keratitis exceeded that in healthy individuals (p < 0.05) and did not significantly differ from those in nondiabetic patients with bacterial keratitis (p > 0.05). Conclusions. DM patients with bacterial keratitis have immunological features of the disease.

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DYSLIPIDEMIA – TYPE2 DIABETES AND NON DIABETES POPULATION IN AND AROUND ERODE DISTRICT, TAMILNADU

10.36106/ijar/4900437 ◽

2021 ◽

pp. 8-9

Author(s):

Murugesan Thinakaran ◽

Thangadurai Chitra

Keyword(s):

Diabetes Mellitus ◽

Cardiovascular Complications ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Lipid Status ◽

Type2 Diabetes ◽

Patients With Diabetes ◽

Tertiary Hospitals ◽

Low Hdl

Background and objects: Patients with diabetes mellitus are at high risk of cardiovascular events because of abnormal lipid status. Dyslipidemia is common in diabetes mellitus and is associated with cardiovascular complications. Early diagnosis and treatment is the main cornerstone in the prevention of its multiple complications. The aim of the study was to determine the prevalence of abnormal lipid prole levels. Materials and Method: The study population was made up of Eighty-ve (85) already diagnosed type 2 diabetic patients and Ninety-two (92) with an age range of 28 to 70 years, who come from routine health, follow up at various tertiary hospitals in Erode. The samples were analyzed using the chemical analyzer COBAS INTEGRA 400. Results and Conclusion: Dyslipidemia was found in 63.52% in type2 diabetes patients and 43.47% in non-diabetic patients. High TG, high LDL-C, high TC and low HDL-C exhibited an increasing trend in the proportion of patients with dyslipidemia. The following risk factors namely female sex, age above 50- years, BMI (overweight and obese), poor glycemic control, central obesity and physical inactivity were associated with diabetic dyslipidemia. This study presents some interesting and novel ndings which may be very important in the care and management of patients with type-2 diabetes.

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