scholarly journals Effect of Simvastatin on the spectrum of blood plasma fat acids change in the patients with ischemia heart disease

2008 ◽  
Vol 7 (3) ◽  
pp. 13-19
Author(s):  
V. V. Novitsky ◽  
R. S. Karpov ◽  
M. Yu. Kotlovsky ◽  
S. V. Klemenkov ◽  
O. V. Voronkova ◽  
...  
Keyword(s):  
Heart Disease ◽  
Blood Plasma ◽  
Total Concentration ◽  
Saturated Fat ◽  
Constant Level ◽  
Simvastatin Treatment ◽  
Total Level ◽  
Total Percentage ◽  
Healthy Donors ◽  
In Blood Plasma

The intake of Simvastatin in a dosage of 40 mg daily within two months in patients with ischemia heart disease has caused increase in the general percentage of ω6 fat acids (FA) at a constant level of ω3 FA, that was not accompanied by change of a ratio ω3/ω6 FA. Despite of progressing increase in total concentration of ω7 FA (at women) and increase of a level ω9 FA (at men) during simvastatin treatment at women and at men led to decrease in the total percentage of nonsaturated fat acids concerning corresponding parameters at healthy donors. The Simvastatin treatment was accompanied by increase in blood plasma in patients with ischemia heart disease (and first of all at women) percentage of the separate saturated fat acids, and also their total level. The index of nonsaturation of fat acids of blood plasma is decreased.

scholarly journals Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus

2012 ◽  
Vol 58 (4) ◽  
pp. 475-484 ◽  
Author(s):  
I.V. Gorudko ◽  
V.A. Kostevich ◽  
A.V. Sokolov ◽  
I.V. Buko ◽  
E.E. Konstantinova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Heart Disease ◽  
Blood Plasma ◽  
Cardiovascular Complications ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Concentration ◽  
Comprehensive Information ◽  
Healthy Donors ◽  
Patients With Diabetes ◽  
In Blood Plasma

Using previously developed spectro-photometrical method (Bioorg. Khim. 2009. V. 35. pp. 629-639), a significant increase of myeloperoxidase (MPO) activity was found in blood plasma of patients with type 2 diabetes mellitus (DM2) without of cardiovascular complications, as well as with ischemic heart disease (IHD). Plasma MPO concentration measured by an enzyme-linked immunosorbent assay was significantly higher only in blood plasma of patient with DM2 and IHD. A direct and significant correlation between MPO activity and MPO concentration was observed only in blood plasma samples from healthy donors. Increased MPO activity did not correlate with MPO concentration in blood plasma of patients with DM2 and DM2 with IHD. Taken together, these results highlight the necessity for studying of the MPO role in the development of pathological processes to determine both the amount of enzyme and its peroxidase activity in the blood. The proposed approach gives comprehensive information about the relationship between MPO activity and MPO concentration in patient blood. Since the high concentration of MPO is a diagnostically significant parameter in the prediction of endothelial dysfunction and cardiovascular disease development, the obtained results evidence the contribution of MPO-dependent reactions in cardiovascular complications associated with diabetes. MPO activity may serve as an additional diagnostic criterion for determination of risk of IHD in DM patients.

scholarly journals Peculiarities of Free-Radical Processes and Neuropsychological Status in Patients with Chronic Generalized Periodontitis and Possibility of Correcting their Impairments with Antioxidant Therapy

2019 ◽  
Vol 20 (2) ◽  
pp. 55-64
Author(s):  
Mihail Saulin ◽  
Sergej Bolevich ◽  
Tatjana Savateva-Liubiova ◽  
Konstantin Sivak ◽  
Ekaterina Silina
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Plasma ◽  
Malonic Dialdehyde ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Healthy Donors ◽  
Surgical Methods ◽  
Antiperoxide Activity ◽  
In Blood Plasma

Abstract This clinical study included a total of 71 patients with chronic generalized periodontitis (CGP). The chemiluminescence method was used to determine the level of generation of reactive oxygen species by leukocytes (basal and stimulated), as well as the content of malonic dialdehyde in blood plasma and antiperoxide activity of plasma. The anxiety level was measured using the Spielberger’s test. Patients with chronic generalized periodontitis in the exacerbation phase were found to have a natural significant increase in both basal and stimulated PICL, as well as an increase in blood plasma malonic dialdehyde 1.6-, 3.9- and 1.4-fold, respectively, compared with apparently healthy donors (all p<0.05), and a significant 2.1-fold decrease in plasma APA compared with healthy donors (p<0.001). In the remission phase, all parameters were normalized, however, they did not reach the norm. We revealed significant dependence of reactive oxygen species generation by leukocytes, lipid peroxidation and antiperoxide activity of plasma on the severity of the course of chronic generalized periodontitis, whereas plasma antiperoxide activity decreased. In patients with the severe course, after the treatment with AO, situational and personal anxiety increased, depression deteriorated, unlike patients with mild-to-moderate course in whom this symptomatology virtually disappeared. In patients with the severe course of periodontitis after surgical manipulations, the parameter in the Hounsfield units increased gradually, on days 14, 30 and 90 on average, 1.3, 2.4 and 3.2-fold, respectively, thus suggesting that bone tissue density increased faster after surgical methods of treatment in patients with severe chronic generalized periodontitis.

SOLUBLE FORMS OF PD-1 AND PD-L1 IN BLOOD PLASMA OF GASTRIC CANCER PATIENTS AND THEIR ASSOCIATIONS WITH CLINICAL AND MORPHOLOGICAL CHARACTERISTICS OF THE DISEASE

2020 ◽  
Vol 65 (6) ◽  
pp. 347-352 ◽  
Author(s):  
E. S. Gershtein ◽  
N. A. Ognerubov ◽  
V. L. Chang ◽  
V. V. Delektorskaya ◽  
E. A. Korotkova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Blood Plasma ◽  
Cancer Patients ◽  
Cell Cancer ◽  
Morphological Characteristics ◽  
Control Group ◽  
Tnm System ◽  
Healthy Donors ◽  
Gastric Cancer Patients ◽  
In Blood Plasma

Results of comparative ELISA investigation of pretreatment sPD-1 and sPD-L1 content in blood plasma of 100 gastric cancer patients at various disease stages aged 25 to 81 years are presented. Control group included 60 practically healthy donors aged 18 - 68 years. Plasma sPD-L1 concentrations did not differ between gastric cancer patients and control group, and sPD-1 levels were statistically significantly lower in patients than in healthy donors (p<0.0001). Positive correlation (R=0.38; p=0.003) was revealed between plasma sPD-1 and sPD-L1 levels in control group and negative (R= -0.26; p=0,009) - in gastric cancer patients. ROC curve revealed the best sPD-1 cut-off level (< 21 pg/ml) with 77% sensitivity and 63.3% specificity, which is not sufficient for its application as diagnostic marker. Statistically significant increase of plasma sPD-L1 from stage I to stage IIIC (R=0.50; p=0.000011) was found. Analysis of associations between the evaluated markers’ levels and indices of gastric cancer expansion according to TNM system revealed statistically significant positive associations of plasma sPD-L1 levels with T (tumor invasion depth) and N (number of affected lymph nodes) indices: R=0.33; p=0.00093, and R=0.27; p=0.0099 respectively. sPD-L1 level was significantly increased in patients with low differentiated adenocarcinoma and cricoid-cell cancer as compared to highly differentiated adenocarcinoma (p=0.02 and p=0.004 respectively); in patients with cricoid-cell cancer it was also higher than in those with moderately differentiated adenocarcinoma (p=0.043) and undifferentiated cancer (p=0.049). Plasma sPD-1 level did not depend on disease stage, TNM system indices and tumor histological structure. Thus, soluble ligand sPD-L1, but not its receptor sPD-1, plasma level is increased in patients with unfavorable clinical and morphological characteristics, may be regarded as potentially valuable prognostic factor for gastric cancer patients’ survival, and probably as a predictor of anti - PD-1/PD-L1 treatment efficiency.

scholarly journals Identification of miRNA-497 and miRNA-27b-5p as potential diagnostic markers of cardiac fibrosis

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
R Tikhomirov ◽  
B Reilly-O'donnell ◽  
C Lucarelli ◽  
S Greco ◽  
G Zacagnini ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cardiac Fibrosis ◽  
Cardiac Fibroblasts ◽  
Smooth Muscle Actin ◽  
Regulation Of Gene Expression ◽  
Control Group ◽  
P Value ◽  
Vast Number ◽  
Healthy Donors ◽  
In Blood Plasma

Abstract Background Cardiac fibrosis is associated with inflammation and extracellular matrix (ECM) accumulation. A pro-fibrotic cytokine, IL11 induces cardiac fibroblasts conversion to myofibroblasts expressing α-smooth muscle actin (α-SMA) and ECM. MicroRNAs (miRNAs) are a class of small non-coding RNAs which participate in regulation of gene expression; Although mainly intracellular, miRNAs can be released into the blood stream where they can be readily detected. Purpose To screen miRNAs upregulated following IL11 triggered conversion of rat cardiac fibroblasts into myofibroblasts. To validate these miRNAs as potential diagnostic biomarkers of cardiac fibrosis by testing their level in blood plasma and septum of aortic valve stenosis (AVS) patients. Methods and results With a bioinformatical approach (Figure 1), we predicted miRNAs which can target proteins involved in TGFβ and IL-11 pathways of fibrosis progression. Of a vast number of miRNAs, we identified 7 strong candidates. After qPCR validation, we found miRNA-27b-5p and miRNA-497 to be significantly upregulated in rat cardiac fibroblasts treated by IL11 (5 ng/ul) but not TGFβ1 (100 ng/ul), values are 2–ΔΔCt: (3±1.5) and (5.2±2.2) (p-value &lt;0.05) for miRNA-27b-5p and miRNA-497 respectively. Next, we overexpressed these two miRNAs separately in rat cardiac fibroblasts. With immunostaining we observed a (18.3±6.8)% increase in the percentage of α-SMA positive cells for miR-27b-5p and a (38.0±8.3)% increase for miR-497. Moreover, we detected with qPCR a significant up-regulation of α-SMA expression (−ΔΔCt = 3.4±0.9 for miR-27b-5p; −ΔΔCt = 8.2±0.7 for miR-497) in cells overexpressing miRNA27b-5p and miRNA497. Furthermore, we found that levels of both miRNA-27b-5p and miRNA-497 were significantly higher in blood plasma (p=0.0002, p=0.04) of AVS patients compared to age and sex matched control group of healthy donors (Figure 2) and heart septum (p=0.0004, p=0.04) of AVS patients compared to septum of healthy donors that could not be used for transplantation. In addition, quantification of Sirius red staining and immunohistochemistry for Col1a1 displayed significant ECM accumulation in AVS patients (p=0.04). Conclusions We found miRNA-497 and miRNA-27b-5p to be pro-fibrotic in rat fibroblasts. Importantly, we found both miRNAs to be up-regulated in the peripheral blood of AVS patients. FUNDunding Acknowledgement Type of funding sources: Other. Main funding source(s): Roman Tikhomirov PhD studentship is supported by a fellowship from the University of Verona, ItalyEEU-Cardiac RNA cost action CA17129

scholarly journals Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis

2020 ◽  
Vol 218 (3) ◽  
Author(s):  
Alex Marki ◽  
Konrad Buscher ◽  
Cristina Lorenzini ◽  
Matthew Meyer ◽  
Ryosuke Saigusa ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Vessel Wall ◽  
Submicron Particles ◽  
New Class ◽  
Vessel Lumen ◽  
Molecular Pattern ◽  
Healthy Donors ◽  
In Blood Plasma

Rolling neutrophils form tethers with submicron diameters. Here, we report that these tethers detach, forming elongated neutrophil-derived structures (ENDS) in the vessel lumen. We studied ENDS formation in mice and humans in vitro and in vivo. ENDS do not contain mitochondria, endoplasmic reticulum, or DNA, but are enriched for S100A8, S100A9, and 57 other proteins. Within hours of formation, ENDS round up, and some of them begin to present phosphatidylserine on their surface (detected by annexin-5 binding) and release S100A8–S100A9 complex, a damage-associated molecular pattern protein that is a known biomarker of neutrophilic inflammation. ENDS appear in blood plasma of mice upon induction of septic shock. Compared with healthy donors, ENDS are 10–100-fold elevated in blood plasma of septic patients. Unlike neutrophil-derived extracellular vesicles, most ENDS are negative for the tetraspanins CD9, CD63, and CD81. We conclude that ENDS are a new class of bloodborne submicron particles with a formation mechanism linked to neutrophil rolling on the vessel wall.

Effect of Cascade Plasma Filtration on Spectrum of Subfractions of Low Density Lipoproteins in Blood Plasma of Patients with Ischemic Heart Disease

Kardiologiia ◽  
2014 ◽  
Vol 4_2014 ◽  
pp. 10-15
Author(s):  
E.A. Utkina Utkina ◽  
O.I. Afanasieva Afanasieva ◽  
I.Yu. Adamova Adamova ◽  
M.V. Ezhov Ezhov ◽  
M.S. Safarova Safarova ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Blood Plasma ◽  
Low Density Lipoproteins ◽  
Ischemic Heart ◽  
Low Density ◽  
Plasma Filtration ◽  
In Blood Plasma

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

EFFECT OF DIETARY LEVELS OF SODIUM AND POTASSIUM ON GROWTH AND ON CONCENTRATIONS IN BLOOD PLASMA AND TISSUES OF WHITE RAT

1950 ◽  
Vol 162 (1) ◽  
pp. 182-188 ◽  
Author(s):  
J. H. Meyer ◽  
R. R. Grunert ◽  
Marie T. Zepplin ◽  
R. H. Grummer ◽  
G. Bohstedt ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Sodium And Potassium ◽  
In Blood Plasma
Sign in / Sign up

Export Citation Format

Share Document