scholarly journals The comparative study of fibrin degradation products in normal pregnancy and pregnancy induced hypertension

2017 ◽  
Vol 6 (4) ◽  
pp. 1258
Author(s):  
Rajani Upadhyaya ◽  
Anitha Sannaboraiah ◽  
Kasturi V. Donimath
Keyword(s):  
Platelet Count ◽  
Normal Control ◽  
Degradation Products ◽  
Normal Pregnancy ◽  
Pregnancy Induced Hypertension ◽  
Coagulation Parameters ◽  
Fibrin Degradation Products ◽  
Induced Hypertension ◽  
Study Population ◽  
D Dimer

Background: Pregnancy induced hypertension is a multisystem disorder and is characterized by changes in haemostatic system. The assessment of the coagulation parameters of the patients of pre-eclampsia and eclampsia is important because it helps to diagnose the severity of the disease, and to predict the outcome. There is no universal agreement as to the need for further investigations if the platelet count comes normal. Hence in such cases it is always prudent to get the coagulation profile. D dimer of such patients is done to diagnose the cases of coagulation failure early and to manage it efficiently. The objectives of the study to compare the Fibrin Degradation Products in term normal pregnancy, pre eclamptic and eclamptic patients, to assess the severity of pregnancy induced hypertension and to detect coagulation failure early and manage before it worsens.Methods: This study was conducted in the Department of Obstetrics and Gynaecology at Karnataka Institute of Medical Sciences, Hubli during the period of March 2014 to February 2015 on 100 patients between 37-42 weeks of gestation. 50 controls were well matched with the study population which included a total of 50 patients with pre-eclampsia and eclampsia. Pregnant women with known bleeding disorders, on anticoagulant therapy, with abruptio placentae, with IUD, in labour and with established DIC were excluded.The blood coagulation parameters which were compared between the control and the study population were Bleeding time (BT), Platelet Count, Clotting time (CT), Prothrombin time (PT), Activated partial thromboplastin time (aPTT) and D-dimer.Results: The BT, CT, PT, aPTT values were nearly identical in all the groups. The platelet count showed a decreasing trend from normal control to eclampsia group. The D dimer showed an increasing trend from the normal control to eclampsia group. D dimer level was raised in all patients who were in sub clinical and clinical coagulation failure.Conclusions: This study shows that even with the normal routine coagulation parameters, D dimer was significantly elevated in both subclinical and clinical DIC. So, D dimer can be used as a specific tool in early diagnosis and deciding appropriate management of PIH.

scholarly journals A Comparative Study of Coagulation Profile and Haematological Parameters in Pregnancy Induced Hypertension (PIH).

2021 ◽  
Vol 7 (3) ◽  
pp. 150-154
Author(s):  
Dr. Chaithra H ◽  
◽  
Dr. Ramesh S T ◽  
Dr. Riyaz Ahmad ◽  
◽  
...  
Keyword(s):  
Platelet Count ◽  
Hematological Parameters ◽  
Data Entry ◽  
Hematological Parameter ◽  
Pregnancy Induced Hypertension ◽  
Control Groups ◽  
Coagulation Parameters ◽  
Induced Hypertension ◽  
Diagnostic Criterion ◽  
In Pregnancy

o evaluate the changes in PIH (Pregnancy Induced Hypertension) by using haematologicaland coagulation parameters like platelet count, MPV, PDW, PT and APTT. Materials and methods: Atotal of 150 cases comprising 75 control groups and 75 cases group (pregnancy-inducedhypertension) were enrolled in the study. Hematological parameters like platelet count, MPV, PDWand coagulation parameters like PT and APTT were studied in these patients. Data entry was done inan excel spreadsheet and by using SPSS (version -20). Results: The hematological parameter -Platelet count was markedly reduced in patients with preeclampsia compared to normal pregnantpatients. MPV, PDW, PT and APTT were increased which is statistically significant. Conclusion: Theabnormalities about hematological and coagulation parameters in preeclampsia are the prognosticmarkers used as an additional diagnostic criterion for preeclampsia in rural hospitals.

scholarly journals Study of coagulation profile and platelet indices in pregnancy induced hypertension with special reference to preeclamptic and eclamptic patients

2020 ◽  
Vol 8 (3) ◽  
pp. 1114
Author(s):  
Biparnak Haldar ◽  
Gopinath Barui
Keyword(s):  
Platelet Count ◽  
Post Partum ◽  
Strong Relationship ◽  
Normal Pregnancy ◽  
Pregnancy Induced Hypertension ◽  
Distribution Width ◽  
Mortality And Morbidity ◽  
Platelet Indices ◽  
Coagulation Profile ◽  
Induced Hypertension

Background: Hypertensive disorders of pregnancy affect about 10% of all pregnant women around the world. A variety of haematological abnormalities may occur in women with Pregnancy Induced Hypertension (PIH), thrombocytopenia being the most common. There is also a definite exaggeration of the hypercoagulable state during PIH. A strong relationship exists between the two most important causes of maternal mortality and morbidity worldwide: Preeclampsia and Post-partum hemorrhage. The aim of this study was to find out the changes that occur in the coagulation profile and platelet indices in PIH as compared to that in normal pregnancy and if they can be used as a reliable indicator of the onset and severity of Preeclampsia and eclampsia.Methods: This was a hospital based analytical prospective study carried out in R. G. Kar Medical College, between January 2017 to June 2018 on 120 patients with PIH. The study parameters included Platelet count, Platelet Distribution Width (PDW), Mean Platelet Volume (MPV), Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT).Results: The results showed significant decrease in platelet count in preeclampsia and eclampsia patients when compared to normotensive patients. Also, there was significant increase in MPV and PDW in preeclamptic and eclamptic patients. Coagulation profile showed increased PT and APTT both in preeclamptic and eclamptic patients with eclamptic patients having significantly higher APTT than other groups.Conclusions: Thus, authors came to the conclusion that platelet indices and coagulation profile can be used as a reliable early indicator of onset and severity of preeclampsia and eclampsia.

MONOCLONAL ANTIBODIES OF THE IgM AND IgG CLASS SPECIFIC FOR CROSSLINKED FIBRIN DEGRADATION PRODUCTS

1987 ◽  
Author(s):  
P J Gaffney ◽  
L J Creighton ◽  
A Curry ◽  
B MacMahon ◽  
R Thorpe
Keyword(s):  
Monoclonal Antibodies ◽  
Thrombolytic Therapy ◽  
Epitope Mapping ◽  
Degradation Products ◽  
Subcutaneous Route ◽  
Fibrin Degradation Products ◽  
Conformational Epitopes ◽  
Immunoglobulin Class Switching ◽  
Unique Specificity ◽  
D Dimer

Monoclonal antibodies (mabs) to crosslinked fibrin degradation products (XL-FDP) having the general formula D/Y[X]nY/D (known as X-oligomer) and D-D (known as D dimer) have been raised in balb/C mice by both a novel mtrasplenic and a conventional subcutaneous route of immunisation and by combinations of both these procedures. Mabs to X-oligomers (NIBn 52 and NIBn 123) obtained by an intrasplenic procedure have been demonstrated to crossreact only with X-oligomer in a 2-site ELISA procedure and not with D dimer or whole fibrinogen and have been shown to be of value m the examination of clinical material obtained from patients with various types of thrombosis and have also been useful in monitoring the efficacy of thrombolytic therapy. The X-oligomer mabs are immunoglobulins of the M class. It was demonstrated that their unique specificity for conformational epitopes on the large X-oligomer fragments does not reside in the IgM structure since alterative immunisation procedures have been used to generate mabs of the IgG class which have the same specificity. Using immunoglobulin class switching in culture rather than during immunisation was suggested by certain cell lines which produced both IgM and IgG specific for X-oligomer. This latter point needs rigorous validation.Immunoglobulin G type mabs to highly purified D dimer were raised by conventional subcutaneous immunisation of balb/C mice. One of these, NIBn-11, was found to crossreact with PVC-immobilised X-oligomer and D dimer but not with fibrinogen. However NIBn-11 did not bind to D dimer in a 2-site ELISA procedure while crossreactmg quite avidly with X-oligomer. This suggests that the D dimer epitope to which NIBn-11 is directed is expressed in some conformations and not m others and that these conformations are always expressed in the complex X-oligomer group of fragments. These mabs, whilst of value in measuring certain unique fibrin fragments m plasma, are useful in the epitope mapping of fibrinogen/fibrin and their plasmm-mediated

scholarly journals D-dimer kit with a High FDP/D-Dimer Ratio is Useful for Diagnosing Thrombotic Diseases

2022 ◽  
Vol 28 ◽  
pp. 107602962110705
Author(s):  
Nozomi Ikeda ◽  
Hideo Wada ◽  
Yuhuko Ichikawa ◽  
Minoru Ezaki ◽  
Motoko Tanaka ◽  
...  
Keyword(s):  
Venous Thromboembolism ◽  
Critically Ill ◽  
Disseminated Intravascular Coagulation ◽  
Critically Ill Patients ◽  
Degradation Products ◽  
Intravascular Coagulation ◽  
Fibrin Degradation Products ◽  
Peripheral Arterial ◽  
Thrombotic Diseases ◽  
D Dimer

Introduction Although D-dimer is a useful biomarker of thrombosis, there are many D-dimer kits, with high and low fibrinogen and fibrin degradation products (FDP)/ D-dimer ratios. Methods Plasma D-dimer levels were measured using three different kits in critically ill patients to examine the usefulness of such measurements for detecting the thrombotic diseases and determining the correlation with the FDP and FDP/D-dimer ratio. Results Although three D-dimer kits showed marked utility for diagnosing disseminated intravascular coagulation (DIC) and peripheral arterial and venous thromboembolism (PAVTE), the D-dimer levels determined using the three kits varied among diseases. Indeed, one D-dimer kit showed a high FDP/D-dimer ratio, and another kit showed a low FDP/D-dimer ratio. D-dimer kit with low FDP/D-dimer ratio tended to have high cut-off values and low specificity for diagnosing DIC and PAVTE. In D-dimer kit with high FDP/D-dimer ratio, FDP/D-dimer ratios in patients with thrombosis was significantly higher than that in patients without thrombosis. Conclusion All three D-dimer kits show utility for detecting thrombotic diseases. However, the D-dimer levels determined using the kits varied due to differences in the FDP/D-dimer ratio. In combination with the FDP level, a D-dimer kit with a high FDP/D-dimer ratio may be useful.

PLASMA LEVELS OF FRAGMENT D-DIMER OF CROSSLINKED FIBRIN DURING THROMBOLYTIC THERAPY WITH RECOMBINANT TISSUE-TYPE PLASMINOGEN ACTIVATOR

1987 ◽  
Author(s):  
P Declerck ◽  
P Mombaerts ◽  
P Holvoet ◽  
D Collen
Keyword(s):  
Thrombolytic Therapy ◽  
Plasma Levels ◽  
Degradation Products ◽  
Fibrin Clot ◽  
Tissue Type ◽  
Fibrin Degradation Products ◽  
Type Plasminogen Activator ◽  
Rate Of Increase ◽  
Significant Difference ◽  
D Dimer

Plasma levels of crosslinked fibrin degradation products (XLDP) were measured before and at the end of the administration of rt-PA (40 to 100 mg over 1.5 to 8 hours) in healthy volunteers (n=5) and patients with deep venous thrombosis (DVT) (n=8), pulmonary embolism (PE) (n=16)and myocardial infarction(MI)(n=10). Determinations were performed using our newly developed ELISA, specific for crosslinked fibrin derivatives, based on two monoclonal antibodies (15C5 and 8D3H2) raised against purified human fragment D-dimer. All plasma samples were collected on citrate and trasylol. Results are expressed as mean and range of D-dimer equivalents (μg/ml).Baseline levels in patients with MI are only slightly elevated. The increased levels inDVT and PE are in agreement with previous studies. After infusion of rt-PA a small increase of XLDP is seen even innormal subjects. A very marked increasof XLDP is detected in patients with PE and DVT but not in patients with MI. This may reflect differences in the amounts of fibrin clot dissolved in these patient groups.No significant correlation was found between the increase of XLDP and success of therapy, although a significant difference in D-dimer levels was formed between the two groups with PE: successful (n=ll): 116 (range 61-192) vs. unsuccessful (n=5): 68 (36-155).Thus, XLDP are already elevated under baseline conditions in patients with DVT and PE and increase very markedly during thrombolytic therapy. The absolute levels after thrombolytic therapy do not strictly correlate with success of therapy. It could be useful to measure D-dimer levels during early stages of therapy, because the rate of increase of XLDP levels might correlate with the efficacy of thrombolytic treatment.

scholarly journals Ouabain-Like Na+, K+-ATPase Inhibitory Activity of a Plasma Extract in Normal Pregnancy and Pregnancy Induced Hypertension

1991 ◽  
Vol 57 (4) ◽  
pp. 571-581
Author(s):  
Hiroko Miyagi ◽  
Makie Higuchi ◽  
Michio Nakayama ◽  
Hidehiko Moromizato ◽  
Matao Sakanashi
Keyword(s):  
Inhibitory Activity ◽  
Normal Pregnancy ◽  
Pregnancy Induced Hypertension ◽  
Induced Hypertension ◽  
Plasma Extract

scholarly journals D-Dimer and Fibrin Degradation Products Impair Platelet Signaling: Plasma D-Dimer Is a Predictor and Mediator of Platelet Dysfunction During Trauma

2020 ◽  
Vol 5 (6) ◽  
pp. 1253-1264
Author(s):  
Christopher C Verni ◽  
Antonio Davila ◽  
Carrie A Sims ◽  
Scott L Diamond
Keyword(s):  
Degradation Products ◽  
Calcium Mobilization ◽  
Factor Xiiia ◽  
Platelet Glycoprotein ◽  
Trauma Patients ◽  
Platelet Dysfunction ◽  
Soluble Fibrin ◽  
Glycoprotein Vi ◽  
Fibrin Degradation Products ◽  
D Dimer

Abstract Background Platelet dysfunction often accompanies trauma-induced coagulopathy. Because soluble fibrin impairs platelet glycoprotein VI (GPVI) signaling and platelets of trauma patients can display impaired calcium mobilization, we explored the role of fibrinolysis on platelet dysfunction during trauma. Methods Convulxin-induced GPVI calcium mobilization was investigated in healthy platelet-rich plasma (PRP) pretreated with thrombin and tissue plasminogen activator (tPA). Blood samples from healthy participants (n = 7) and trauma patients (n = 22) were tested for platelet calcium mobilization, plasma D-dimer, platelet D-dimer binding (via flow cytometry), and platelet lumi-aggregometry. Results For healthy platelets, maximal platelet dysfunction was observed when cross-linked soluble fibrin (no tPA) or cross-linked fibrin degradation products (FDPs) were generated in suspension before convulxin stimulation. Lack of fibrin polymerization (inhibited by Gly-Pro-Arg-Pro [GPRP]) or lack of factor XIIIa cross-linking (T101-inhibited) restored GPVI signaling, whereas non–cross-linked FDPs only partially blocked signaling induced by convulxin. In addition, D-dimer added to healthy PRP impaired platelet aggregation and dense granule release induced by various agonists. Plasma D-dimer level was strongly correlated (R = 0.8236) with platelet dysfunction as measured by platelet calcium mobilization induced with various agonists. By 48 to 120 h after trauma, plasma D-dimer levels declined, and platelet function increased significantly but not to healthy levels. Trauma platelets displayed elevated D-dimer binding that was only partially reduced by αIIbβ3-inhibitor GR144053. After 60-minute incubation, washed healthy platelets resuspended in plasma from trauma patients captured approximately 10 000 D-dimer equivalents per platelet. Conclusions During trauma, D-dimer and FDPs inhibit platelets, potentially via GPVI and integrin αIIbβ3 engagement, contributing to a fibrinolysis-dependent platelet loss-of-function phenotype.

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