scholarly journals Ascorbic acid irrigation during debridement decreased the colony count of Staphylococcus aureus and interleukin-6 blood count in grade ii open fracture of long bones

2020 ◽  
Vol 8 (9) ◽  
pp. 3194
Author(s):  
I. Ketut Suyasa ◽  
Putu Astawa ◽  
Mulyadi Ridia ◽  
Trimanto Wibowo
Keyword(s):  
Staphylococcus Aureus ◽  
Ascorbic Acid ◽  
Serum Level ◽  
Open Fracture ◽  
Colony Count ◽  
Control Group ◽  
Long Bones ◽  
Acid Irrigation ◽  
Before And After ◽  
Grade Ii

Background: Infection in open fractures is still a problem that cannot be fully managed. Various types of studies have been conducted to find an effective and efficient solution to wash open fracture wounds. This study compared the effectiveness of ascorbic acid irrigation in reduction of Staphylococcus aureus colony and interleukin-6 (IL-6) serum level as systemic inflammation marker in debridement procedure of grade II Gustilo-Anderson open fracture of long bones.Methods: This study was an experimental study with pre-post-test control group design. This study included 24 subjects aged 15-75 years old with Gustillo-Anderson type II open fractures which were divided into two groups. The treatment group had additional irrigation by using 10 mg/ml ascorbic acid solution during debridement, whereas the control group had debridement only without irrigation with ascorbic acid. The culture and colony count of Staphylococcus aureus and IL-6 serum level was obtained and measured before and after the treatment in both groups. Statistical analysis (Wilcoxon test) was performed to compare the difference of the two parameters (Δ colony count and Δ IL-6) before and after the treatment.Results: There were no significant difference in Δ colony count of Staphylococcus aureus (p=0.308) and ΔIL-6 serum level (p=0.239) between the control group and treatment group.Conclusions: Ascorbic acid irrigation was not proved to decrease the colony count of Staphylococcus aureus and IL-6 serum level in grade II open fracture of long bones.

Changes in the serum level of the trace elements in chronic renal

2021 ◽  
Vol 2 (3) ◽  
pp. 402-409
Author(s):  
Baghdad Science Journal
Keyword(s):  
Trace Elements ◽  
Serum Level ◽  
Blood Serum ◽  
Healthy People ◽  
Control Group ◽  
Before And After ◽  
Iron And Zinc

Were studied changes in the concentration of copper, iron and zinc in blood serum of one hundred patients with chronic kidney and treated dialysis blood were also measured the level of calcium kidney and phosphate Calciotropic in serum of these patients took samples of blood from these patients before and after treatment dialysis vessels as well as the statement of changes in those standards Alkimaahiatih Results were compared with twenty-five healthy people (control group)

scholarly journals Effectiveness of Surface Cleaning and Disinfection in a Brazilian Healthcare Facility

2018 ◽  
Vol 12 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Aires G. Santos-Junior ◽  
Adriano M. Ferreira ◽  
Oleci P. Frota ◽  
Marcelo A. Rigotti ◽  
Larissa da S. Barcelos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Adenosine Triphosphate ◽  
Healthcare Facility ◽  
Surface Cleaning ◽  
Point Of View ◽  
Colony Count ◽  
Before And After ◽  
Bioluminescence Assay ◽  
Cleaning And Disinfection ◽  
Hospital Cleaning

Background: Failures in the processes of cleaning and disinfecting health service surfaces may result in the spread and transfer of pathogens that are often associated with healthcare-related infections and outbreaks. Aims: To assess the effectiveness of environmental surface cleaning and disinfection in a hospital clinic. Method: The study was conducted in a nursing ward with 45 beds. A total of 80 samples from five high-touch surfaces were evaluated before and after cleaning and disinfection, using the following methods: visual inspection, adenosine triphosphate bioluminescence assay, aerobic colony count, Staphylococcus aureus colony count, and evaluation of resistance to methicillin. The data analysis used nonparametric comparative and correlative tests to observe any differences in the pre- and post- cleaning and disinfection results for the surfaces assessed. Results: Effective cleaning and disinfection had a significant effect on only two surfaces when measured for the presence of adenosine triphosphate, the inner bathroom door handle (p=0.007) and the toilet bowl (p=0.01). When evaluated for Staphylococcus aureus colony count, the toilet flush handle also demonstrated a significant effect (p=0.04). Conclusion: The effectiveness of cleaning and disinfection of the surfaces tested was not satisfactory. An educational intervention is recommended for the cleaning and disinfection staff and the nursing team at the healthcare facility. Relevance to Clinical Practice: The data in the study revealed that daily hospital cleaning and disinfection in the sampled sites are not sufficient in medical and surgical wards. Hospital cleanliness must be reevaluated from the point of view of materials, such as an adequate supply of clean cloths, in addition to establishing more precise cleanliness protocols and accurate monitoring systems.

scholarly journals The Effect Of Model CRH By Board Reward On Student Learning Outcomes Of Class II In Elementary School

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Rahmawatin Khasanah ◽  
Haris Supratno ◽  
M. Bambang Edi Siswanto
Keyword(s):  
Elementary School ◽  
Student Learning ◽  
Learning Outcomes ◽  
Student Learning Outcomes ◽  
Control Group ◽  
Intact Group ◽  
Before And After ◽  
The Media ◽  
Grade Ii ◽  
Experimental Group

This study applies the model course review horay (CRH) with the help of  media board reward on the theme of learning 6 caring for animals and plants in SDN Jatirejo. The method used is an experimental method in Pre-Experimental Design in the form of Intact-Group Comparison. The subjects of the study were of grade II SDN Jatirejo in academic year 2019/2020. Data collection through observation of learning activities and written tests to measure student learning outcomes before and after being given treatment. The results of data analysis using the t-test formula to determine the effect of treatment in the experimental and control groups. The results of the study using the model course review horay helped by the media reward board were more fun and students were actively learning so that the teacher easily delivered teaching material with a very good category namely the experimental group (11,843 ) > the control group (11,655) which means that there is an influence on the use of the model course review horay with the help of  media board reward in the experimental group is greater than conventional learning applied to the control group. The results of this study as a source of information input and consideration for institutions involved in learning theme 6 grade II elementary school.

scholarly journals THE COMBINATION OF EXERCISE AND ASCORBIC ACID DECREASE BLOOD GLUCOSE LEVEL AND TEND TO AMELIORATE PANCREATIC ISLETS AREA ON HIGH CARBOHYDRATE DIET RATS

2019 ◽  
pp. 20-24
Author(s):  
LILIK HERAWATI ◽  
LINA LUKITASARI ◽  
RIMBUN RIMBUN ◽  
BAMBANG PURWANTO ◽  
GADIS MEINAR SARI
Keyword(s):  
Ascorbic Acid ◽  
Blood Glucose ◽  
Control Group ◽  
Protective Effects ◽  
Duration Of Treatment ◽  
Treatment Groups ◽  
High Carbohydrate ◽  
Significant Difference ◽  
Before And After ◽  
Decrease Blood Glucose

Objective: This study is conducted to determine the protective effects of physical exercise and ascorbic acid on increasing blood glucose (BG) levels and islet pancreatic area in high-carbohydrate (HC) diet rats. Methods: A total of 20 rats were divided into four groups: Control group which was a HC and treatment groups which were HC plus exercise (HCEx), HC plus ascorbic acid (HCAs), and HCEx and ascorbic acid (HCExAs). The duration of treatment was 9 weeks. Swimming to exercise held 6 times a week and ascorbic acid dose was 9 mg. Results: It showed that the smallest body weight was HCEx group. BG difference (before and after treatment = BG diff) had a significant difference (p=0.021) among groups, and the lowest level of BG diff was HCEx group. HCAs had the biggest BG diff. However, there was no significantly difference among groups on islet pancreatic area, but HC group had the largest area. Conclusion: This study suggests that a combination of exercise and ascorbic acid on HC diet subject may regulate BG level compared to the exercise or ascorbic acid alone. However, they do not influence pancreatic islet area.

scholarly journals The Effect Of Model CRH By Board Reward On Student Learning Outcomes Of Class II In Elementary School

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Rahmawatin Khasanah ◽  
Haris Supratno ◽  
M. Bambang Edi Siswanto
Keyword(s):  
Elementary School ◽  
Student Learning ◽  
Learning Outcomes ◽  
Student Learning Outcomes ◽  
Control Group ◽  
Intact Group ◽  
Before And After ◽  
The Media ◽  
Grade Ii ◽  
Experimental Group

This study applies the model course review horay (CRH) with the help of  media board reward on the theme of learning 6 caring for animals and plants in SDN Jatirejo. The method used is an experimental method in Pre-Experimental Design in the form of Intact-Group Comparison. The subjects of the study were of grade II SDN Jatirejo in academic year 2019/2020. Data collection through observation of learning activities and written tests to measure student learning outcomes before and after being given treatment. The results of data analysis using the t-test formula to determine the effect of treatment in the experimental and control groups. The results of the study using the model course review horay helped by the media reward board were more fun and students were actively learning so that the teacher easily delivered teaching material with a very good category namely the experimental group (11,843 ) > the control group (11,655) which means that there is an influence on the use of the model course review horay with the help of  media board reward in the experimental group is greater than conventional learning applied to the control group. The results of this study as a source of information input and consideration for institutions involved in learning theme 6 grade II elementary school.

Study of Viridans Streptococci and Staphylococcus Species in Cleft Lip and Palate Patients before and after Surgery

2005 ◽  
Vol 42 (3) ◽  
pp. 277-279 ◽  
Author(s):  
Erry Mochamad Arief ◽  
Zeehaida Mohamed ◽  
Fauziah Mohamad Idris
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Colony Count ◽  
Control Group ◽  
Viridans Streptococci ◽  
Cotton Swab ◽  
Normal Children ◽  
Streptococcus Oralis ◽  
Before And After ◽  
Sterile Cotton Swab

Objective To determine the effect of surgery on types and colony count of Streptococcus and Staphylococcus species in cleft lip and palate (CLP) patients. Design Saliva samples were collected after the morning meal by placing a sterile cotton swab in the vestibule of the oral cavity from cleft lip and palate patients immediately preoperative and 12 weeks postoperative. Normal children were examined as a control group. Samples were cultured; Staphylococcus and Streptococcus isolates were identified and quantified. Patients Fifteen cleft lip and palate patients and 22 normal children, aged 3 to 39 months were examined. Results Streptococcus mitis biovar 1, Streptococcus salivarius and Streptococcus oralis of the viridans group of streptococci were the most commonly found in normal children, as well as in cleft lip and palate children. In the cleft lip and palate group, mean streptococcal count was 32.41 (29.80) and 46.46 (42.80) in the pre- and postoperative periods, respectively; in the normal group, the count was 20.93 (27.93) and 49.92 (34.72) at 0 week and 12 weeks, respectively. Staphylococcus aureus was the most common Staphylococcus species found in CLP patients, representing 47.4% postoperatively. In the cleft lip and palate children, mean staphylococcal count was 5.34 (8.13) and 0.56 (0.92) in the pre- and postoperative periods, respectively; in normal children, the count was 0.82 (1.98) and 0.60 (2.55) at 0 and 12 weeks, respectively. The differences were statistically significant only for the staphylococcal count between pre- and postoperative periods in children with cleft lip and palate as tested by analysis of variance (p < .05). Conclusions Cleft lip and palate patients had more colonization by S. aureus compared with normal children, and the colony count decreased significantly following surgical repair of the cleft lip and palate.

scholarly journals Changes in the serum level of the trace elements in chronic renal

2005 ◽  
Vol 2 (3) ◽  
pp. 402-409
Author(s):  
Baghdad Science Journal
Keyword(s):  
Trace Elements ◽  
Serum Level ◽  
Blood Serum ◽  
Healthy People ◽  
Control Group ◽  
Before And After ◽  
Iron And Zinc

Were studied changes in the concentration of copper, iron and zinc in blood serum of one hundred patients with chronic kidney and treated dialysis blood were also measured the level of calcium kidney and phosphate Calciotropic in serum of these patients took samples of blood from these patients before and after treatment dialysis vessels as well as the statement of changes in those standards Alkimaahiatih Results were compared with twenty-five healthy people (control group)

scholarly journals Efficacy of Titanium Brush, 915 nm Diode Laser, Citric Acid for Eradication of Staphylococcus Aureus from Implant Surfaces

2021 ◽  
Author(s):  
Mohammad Reza Karimi ◽  
Behshad Farkhondemehr ◽  
Motahare Ghaeni Najafi ◽  
Ardavan Etemadi ◽  
Nasim Chiniforush
Keyword(s):  
Staphylococcus Aureus ◽  
Citric Acid ◽  
Diode Laser ◽  
Positive Control ◽  
Colony Count ◽  
Control Group ◽  
Control Groups ◽  
Mini Implants ◽  
Significant Difference ◽  
Post Hoc

Abstract Background: This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants.Materials and Methods: Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n=12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n=12; chlorhexidine mouthwash (CHX)] and negative [n=2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n=1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal-Wallis and Dunn post-hoc tests.Results: Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal-Walli test revealed a significant difference between the groups regarding the colony count (P<0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P<0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P>0.077).Conclusions: Maximum reduction in colony count was noted in the positive control group. Combination of titanium brush and laser was also effective for decreasing the colony count; however, combined use of titanium brush and citric acid yielded superior results compared to other groups.

scholarly journals Uji Konsentrasi Hambat Minimum (KHM) Getah Kulit Buah Pisang Goroho (Musa acuminafe L.) terhadap Pertumbuhan Staphylococcus aureus

e-GIGI ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Kleysia D. Wuon ◽  
Damajanty H.C. Pangemanan ◽  
P. S. Anindita
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Test Methods ◽  
Banana Peel ◽  
Control Group ◽  
Microbiology Laboratory ◽  
Control Group Design ◽  
Study Program ◽  
Group Design ◽  
Before And After

Abstract: Staphylococcus aureus is the most dominant cause of odontogenuic abscess, albeit, it is resistant to various types of antibiotics. The sap of goroho banana peel (Musa acuminafe L.) contains antibacteria compounds inter alia flavonoid, saponin, and tannin. This study was aimed to determine the minimal inhibitory concentration (MIC) of the sap of goroho banana peel to the growth of Staphylococcus aureus. This was a true experimental study with a randomized pretest-posttest control group design. We used serial dilution with turbidimetry and spectrophotometry as the test methods. Goroho banana was taken from Kelurahan Winangun, Kecamatan Malalayang meanwhile S. aureus bacteria were obtained from Microbiology Laboratory of Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The result of turbidimetry after incubation showed that the tube of 50% of goroho sap looked clear. Measuring absorbance values before and after incubation using spectrophotometer UV-Vis showed that the MIC of the goroho sap was at 25%. Conclusion: The MIC of the sap of goroho banana peel (Musa acuminafe L.) to the growth of Staphylococcus aureus was at concentration of 25%.Keywords: sap of goroho banana peel (Musa acuminafe L.), S. aureus, MIC Abstrak: Staphylococcus aureus merupakan bakteri penyebab abses odontogenik yang paling dominan. Saat ini S. aureus telah resisten terhadap berbagai jenis antibiotik. Getah kulit buah pisang goroho (Musa acuminafe L.) merupakan tanaman herbal yang mengandung senyawa antibakteri, yaitu flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimum (KHM) getah kulit buah pisang goroho terhadap pertumbuhan S. aureus. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan yaitu metode serial dilusi dengan pengujian turbid-metri dan spektrofotometer. Pisang goroho diperoleh dari Kelurahan Winangun, Kecamatan Malalayang. Bakteri S. aureus diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian turbid-metri sesudah inkubasi menunjukkan bahwa tabung dengan konsentrasi getah kulit buah pisang goroho 50% terlihat mulai jernih. Pengukuran nilai absorbansi sebelum dan sesudah inkubasi menggunakan spektrofotometer UV-Vis menunjukkan bahwa KHM getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri S. aureus terdapat pada konsentrasi 25%. Simpulan: Konsentrasi hambat minimum getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri S. aureus terdapat pada konsentrasi 25%Kata kunci: getah kulit buah pisang goroho (M. acuminafe), S. aureus, KHM

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