scholarly journals Contamination of ‘in-use’ bar soaps in dental clinics of University hospital

2021 ◽  
Vol 8 (12) ◽  
pp. 6061
Author(s):  
Jyoti Sharma ◽  
Manjula Mehta ◽  
Sonia Bhonchal Bharadwaj
Keyword(s):  
University Hospital ◽  
Liquid Soap ◽  
Effective Mechanism ◽  
Gram Negative ◽  
Health Care Settings ◽  
Dental Clinics ◽  
Route Of Transmission ◽  
Dental Hospital ◽  
Spread Of Infection ◽  
Contact Free

Hand carriage of bacteria is an important route of transmission of infection in health care settings. Hand washing continues to be the most effective mechanism of prevention of the spread of infection in hospitals. In this study the ‘in-use’ bar soaps in the dental hospital were sampled to look for the presence of microorganisms on their surfaces. All the soaps which were sampled yielded the growth of microorganisms on them. The isolated microorganisms are Bacillus, Micrococci, Staphylococci and gram-negative bacilli. Since the use of ‘in-use’ bar soaps function as reservoir of microorganisms so contact free soap dispensers, soap strips or liquid soap should be used in hospital settings to prevent the transmission of microorganisms.

Challenges in prevention & control of COVID - 19 pandemic in dentistry: A review. (Preprint)

2020 ◽  
Author(s):  
Sameera Begum ◽  
Riaz Abdulla ◽  
Akhter Hussain
Keyword(s):  
Healthcare Professionals ◽  
Preventive Measures ◽  
Health Concern ◽  
Front Line ◽  
Public Health Concern ◽  
College Hospital ◽  
Dental Clinics ◽  
Dental Procedures ◽  
Spread Of Infection ◽  
High Chance

UNSTRUCTURED The menace of COVID 19 pandemic has become a major public health concern all over the world. It is a pandemic outbreak that originated from Wuhan, Hubei province of China in December 2019. All healthcare professionals including dental surgeons are in the front line and a high chance of constantly getting infected. Droplet and aerosol transmissions are the utmost concern in dental clinics and dental college hospitals. Hence, COVID 19 has a high risk of spread through droplets and aerosols generated during dental procedures from infected patients. This review article highlights the dental perspective and discusses the various preventive measures undertaken to control the spread of infection in dental clinics and dental college hospital setups.

scholarly journals 832. Assessment of Risk Factors Associated with Wide-resistance Gram-negative Bacteria Infections

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S457-S457
Author(s):  
Henry Pablo Lopes Campos e Reis ◽  
Ana Beatriz Ferreira Rodrigues ◽  
Julio César Castro Silva ◽  
Lia Pinheiro de Lima ◽  
Talita Lima Quinaher ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mechanical Ventilation ◽  
Univariate Analysis ◽  
Multidrug Resistant ◽  
University Hospital ◽  
Gram Negative Bacteria ◽  
Multiresistant Pathogens ◽  
Gram Negative ◽  
Contact Isolation ◽  
Bladder Catheter

Abstract Background Enterobacteria and multidrug-resistant non-fermenting Gram-negative bacilli present a challenge in the management of invasive infections, leading to mortality rates due to their limited therapeutic arsenal. The objective of this work was to analyze risk factors that may be associated with these infections, for a better situational mapping and assertive decision-making in a university hospital in Brazil. Methods The study was conducted between January and September 2019, with 167 patients in contact isolation at a university hospital in Brazil. Potential outcome-related variables for wide-resistance Gram-negative bacteria (BGN) infections were evaluated. Risk factors were identified from univariate statistical analysis using Fisher’s test. Results 51 (30.5%) out of 167 patients in contact isolation evolved with wide-resistance BGN infection. Risk factors in univariate analysis were age, hospital unit and previous use of invasive devices. Patients aged up to 59 years were more likely to progress to infection than those aged over 60 years (p 0.0274, OR 2.2, 95% CI 1.1-4.5). Those admitted to the oncohematology (p < 0.001, OR 32.5, Cl 9.1-116.3) and intensive care unit (p < 0.001, OR 28.0, Cl 3.5-225.9) units were more likely to develop this type of infection. The least likely were those admitted to a kidney transplant unit (p 0.0034, OR 15.33, Cl 1.8-131.0). Prior use of mechanical ventilation (p 0.0058, OR 12.2, Cl 2.0-76.1) and delayed bladder catheter (p 0.0266, OR 5.0, Cl 1.2-20.1) in patients with respiratory and urinary tract infection, respectively, were also reported as risk factors related to these infections. The gender of the patients was not significant for the study. Conclusion This study determined that variables such as age, hospitalization unit, use of mechanical ventilation and delayed bladder catheter could be considered important risk factors in triggering the infectious process by wide-resistant gram-negative bacteria. Thus, the analysis of these factors becomes a great foundation to prevent the development of multiresistant pathogens through prevention strategies, prophylaxis management and more targeted empirical therapies. Disclosures All Authors: No reported disclosures

High Level of Colistin Resistant Gram-negative Bacteria in a University Hospital in Bulgaria

2021 ◽  
Author(s):  
Yulia Marteva-Proevska ◽  
Tsvetan Velinov ◽  
Rumyana Markovska ◽  
Dilana Dobrikova ◽  
Liudmila Boyanova ◽  
...  
Keyword(s):  
University Hospital ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
High Level

scholarly journals Carriage of ESBL-producing Gram-negative bacteria by flies captured in a hospital and its suburban surroundings in Ethiopia

2020 ◽  
Author(s):  
Tafese B Tufa ◽  
Andre Fuchs ◽  
Tobias Wienemann ◽  
Yannik Eggers ◽  
Sileshi Abdissa ◽  
...  
Keyword(s):  
Waste Disposal ◽  
Neonatal Intensive Care ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Local Data ◽  
Gram Negative ◽  
Disposal Area ◽  
Route Of Transmission ◽  
The Town ◽  
Orthopedic Ward

Abstract Background: Local data from the Asella Teaching and Referral Hospital in the town of Asella, Ethiopia reveal a high prevalence of extended-spectrum β-lactamase- (ESBL) producing Gram-negative bacteria (GNB) in clinical isolates. To investigate a possible route of transmission, we determined the proportions ESBL-producing GNB in isolates from flies caught in the hospital and in the town of Asella. Methods: Flies were collected in August 2019 from the neonatal intensive care unit (NICU), the orthopedic ward, the hospital’s waste disposal area, and from a butchery situated 1.5 km from the hospital. After trapping, the flies were macerated and suspended in sterile normal saline. The suspensions were inoculated on MacConkey agar and incubated overnight. Species identification and antimicrobial susceptibility testing were performed using Vitek®-MS and VITEK® 2. Results: In total, 103 bacterial isolates were obtained from 85 flies (NICU: 11 isolates from 20 flies, orthopedic ward: 10 isolates from 12 flies, waste disposal area: 37 isolates from 26 flies, butchery: 45 isolates from 27 flies). The proportions of ESBL-producing bacteria among isolates obtained from flies collected in the hospital compound were significantly higher (82%, 90%, and 57% in NICU, orthopedic ward and waste disposal area, respectively) compared to flies collected outside of the hospital compound (2% (1/45) in the butchery) (p≤0.001). The proportion of ESBL was 67% (6/9) among Raoultella spp. 67% (4/6) among Kluyvera spp., 56% (5/9) among Enterobacter spp., 50% (5/10) among E. coli, and 44% (8/18) among Klebsiella spp.. Of the 40 ESBL-genes detected, 85% were CTX-M-like, 83% TEM-like, 23% SHV-like, and 2% CTX-M-2-like. ESBL-producing bacteria showed higher rates of resistance against ciprofloxacin (66% vs. 5%), gentamicin (68% vs. 3%), piperacillin-tazobactam (78% vs. 5%), and trimethoprim-sulfamethoxazole (88% vs. 16%), compared to non-ESBL-producing bacteria. Conclusion: A high proportion of ESBL was identified in isolates from flies caught in the hospital compound compared with isolates of flies collected at a distance of 1.5 km from the hospital. Flies can be potential vectors for transmission of multidrug-resistant (MDR) bacteria within hospitals. Further studies are needed to determine the source of MDR colonization in flies and possible impact of MDR for nosocomial infections.

scholarly journals 2143. Attempting to Add Clarity to “Indeterminates” on a Deployed Rapid Diagnostic with Antimicrobial Stewardship Program (ASP) Intervention

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S726-S726
Author(s):  
Heather L Cox ◽  
April E Attai ◽  
Allison M Stilwell ◽  
Kasi B Vegesana ◽  
Frankie Brewster ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Diagnostic Testing ◽  
Bloodstream Infections ◽  
Empiric Therapy ◽  
Central Line ◽  
Blood Cultures ◽  
University Hospital ◽  
Salmonella Spp ◽  
Gram Negative ◽  
Stewardship Program

Abstract Background Rapid diagnostic testing paired with ASP intervention optimizes therapy and improves outcomes but few data guide ASP response in the absence of organism identification (ID). We describe the microbiology for organisms unidentified by Accelerate Pheno™ Gram-negative platform (AXDX) in order to inform ASP-provider team communication (PTC). Methods Consecutive, non-duplicate inpatient blood cultures with Gram-negative bacilli (GNB) following AXDX implementation at a single university hospital between April 2018 and March 2019 were included. Standard of care (SOC) ID and susceptibility followed AXDX. Clinical Microbiology emailed AXDX results to the ASP in real time; results were released into the EMR paired with telephone PTC or withheld after ASP review. Bloodstream Infections (BSIs) and patient outcomes for organisms labeled no/indeterminate ID by the AXDX were characterized. Results AXDX was performed on 351 blood cultures. Among 52 (15%) labeled no/indeterminate ID, SOC methods revealed: Enterobacteriaceae (40%; 9 monomicrobial with AXDX targets), anaerobes (21%), non-lactose fermenters (NLFs) other than Pseudomonas aeruginosa (21%), and fastidious GNB (10%). Frequent organisms without AXDX targets included: Raoultella planticola (4); Bacteroides fragilis, Cupriavidus spp., Haemophilus spp., Prevotella spp., Providencia spp., non-aeruginosa Pseudomonas spp., Salmonella spp. (3 each); Pasteurella multocida, Stenotrophomonas maltophilia (2 each). BSI sources were most commonly intra-abdominal (21%), central line-associated (17%), or unknown (17%). CLABSIs were associated with immune suppression and/or substance abuse in all but 1 case. BSIs without active empiric therapy included: NDM-producing Providencia stuartii SSSI; OXA-48-producing R. planticola intraabdominal infection (IAI); Pandoraea spp. CLABSI after liver transplant; enteric fever; B. fragilis, Leptotrichia wadei, and S. maltophilia, each of unknown source. In-hospital mortality occurred in 4 of these cases. Conclusion When AXDX yields no/indeterminate ID, ASP chart review for possible anaerobic/IAI, unique environmental exposures, and travel history may assist in guiding empiric therapy. GNB with AXDX targets are not excluded. Disclosures All authors: No reported disclosures.

scholarly journals Complete Genome Sequence of Escherichia coli GW-AmxH19, Isolated from Hospital Wastewater in Greifswald, Germany

2020 ◽  
Vol 9 (21) ◽  
Author(s):  
Dominik Schneider ◽  
Daniela Zühlke ◽  
Tabea Petscheleit ◽  
Anja Poehlein ◽  
Katharina Riedel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
University Hospital ◽  
Hospital Wastewater ◽  
Circular Chromosome ◽  
Protein Coding ◽  
Gram Negative ◽  
Content Type ◽  
Protein Coding Genes ◽  
Potential Association ◽  
Tract Infections

ABSTRACT The Gram-negative and rod-shaped Escherichia coli strain GW-AmxH19 was isolated from university hospital wastewater in Greifswald, Germany. The genome consists of two replicons, including one circular chromosome (5.04 Mb) and a circular plasmid (126.96 kb). The genome harbors 4,694 protein-coding genes, comprising multidrug resistance and a potential association with urogenital tract infections.

scholarly journals CONSUMPTION AND BACTERIAL RESISTANCE TO AMINOGLYCOSIDES AT SUDANESE UNIVERSITY HOSPITAL

2018 ◽  
Vol 11 (8) ◽  
pp. 434
Author(s):  
Einas A Bakheit ◽  
Kamal M Elhag ◽  
Abduelmula R Abduelkarem ◽  
Nada A Basheer
Keyword(s):  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Anatomical Therapeutic Chemical ◽  
Statistical Significance ◽  
Linear Regression Analysis ◽  
University Hospital ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Defined Daily Doses ◽  
Hospital Wards

Objectives: The objective of this study was to describe patterns of antimicrobial resistance to gentamicin (Gen) and amikacin (Ak) among Gram-negative aerobic bacteria during 1-year period and to determine the association between antibiotic resistance and the consumption of Gen.Methods: Aminoglycosides consumption at Soba University Hospital wards was measured and susceptibility of Gram-negative bacteria for the same period was evaluated. Consumption data were converted to defined daily doses (DDDs)/100 bed days based on DDD/anatomical therapeutic chemical the WHO system. The association between the frequency of strains resistant to Gen and Ak and their consumption was assessed by linear regression analysis using Spearman’s correlation. The level of statistical significance was set at p<0.05.Results: A total of 973 Gram-negative isolates were identified and tested for antimicrobial susceptibility to Gen and Ak. Resistance to Gen alone was found to be 19.42%; n=189, resistance to Ak alone was found to be 3.08%; n=30, and resistance to Gen plus Ak was found to be 5.24%; n=51. Pseudomonas aeruginosa was the most resistant pathogen to Ak plus Gen (2.26%; n=22). A positive correlation between the increases in the use of Gen and the prevalence of bacterial resistance among hospital wards was found (correlation coefficient r=0.6; p=0.04).Conclusion: Gen and Ak are still highly active antimicrobial agents for the treatment of aerobic Gram-negative bacteria at times of intensified resistance to other antimicrobial agents. Monitoring the use of aminoglycosides is very important too. 

scholarly journals 1214. Microbiological and Clinical Effectiveness of Pulsed-Xenon Ultraviolet Disinfection of the Patient Room in the ICU

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S436-S437
Author(s):  
Keita Morikane ◽  
Shoko Suzuki ◽  
Jun Yoshioka ◽  
Jun Yakuwa ◽  
Masaki Nakane
Keyword(s):  
Clinical Effectiveness ◽  
Healthcare Setting ◽  
Baseline Period ◽  
University Hospital ◽  
Intervention Period ◽  
Tertiary Referral Hospital ◽  
Contact Precautions ◽  
Healthcare Settings ◽  
Route Of Transmission ◽  
The Us

Abstract Background Contact precautions do not necessarily control transmission of MDROs as other route of transmission such as patient environment may be significant. No-touch environmental disinfection has been highlighted in the past several years to control transmission of MDROs. The effectiveness of portable Pulsed Xenon Ultraviolet (PX-UV) device has been examined and demonstrated in US healthcare settings. However, its effectiveness outside the US healthcare setting is seldom reported. Methods This study was conducted in the intensive care unit (ICU) of Yamagata University Hospital, a 637-bed tertiary referral hospital. The ICU has six rooms and beds. In the baseline period (August 2016 to January 2018), all rooms were manually cleaned after every patient transfer/discharge. In the intervention period (February 2018 to February 2019), PX-UV disinfection was added after the manual cleaning. In both periods, all patients were screened for MRSA and two drug-resistant Acinetobacter baumannii (2DRA) to detect acquisition of those pathogens in the ICU. For microbiological evaluation, surfaces were selected for sampling by contact plates before/after manual cleaning and after PX-UV. After overnight incubation, colonies on the plates were counted. Results The incidence of newly acquired MRSA declined over time (1.40 per 1,000 patient-days in the baseline period to 0.95 in the intervention period, relative risk (RR): 0.68, 95% confidence interval (CI): 0.12–3.70). The incidence of newly acquired 2DRA further declined (4.91 to 1.90, RR: 0.39, 95% CI: 0.13–1.18). Notably, no new acquisition of 2DRA was observed since August 2018 for more than 7 months, not only in the ICU but also throughout the hospital. The total count of colonies in the sampling of 140 sites after 17 patient discharges were 3,540 (before manual cleaning), 669 (after manual cleaning, before PX-UV) and 261 (after PX-UV). The percent reduction of microbiological burden by manual cleaning was 81%, but a further 61% reduction was achieved by PX-UV. Conclusion PX-UV is effective in further reducing the microbial burden even after through manual cleaning, which presumably led to termination of transmission of 2DRA in our hospital. The effectiveness of PX-UV in controlling MDROs in the non-US healthcare settings is suggested. Disclosures All authors: No reported disclosures.

scholarly journals Occurrence of unusual non-fermentative gram negative bacilli in intensive care units of a university hospital, Egypt

2016 ◽  
Vol 17 (4) ◽  
pp. 256
Author(s):  
E.M. EL-Behedy ◽  
M Hend ◽  
MM EL-Arini ◽  
M.A. Gerges ◽  
N.A.E. Mohamed ◽  
...  
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
University Hospital ◽  
Gram Negative

scholarly journals The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2) in Dentistry. Management of Biological Risk in Dental Practice

2020 ◽  
Vol 17 (9) ◽  
pp. 3067 ◽  
Author(s):  
Roberto Lo Giudice
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Clinical Signs ◽  
World Health ◽  
Dental Practice ◽  
Risk Of Infection ◽  
Specific Protocol ◽  
Route Of Transmission ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Spread Of Infection

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a novel coronavirus first identified in Wuhan, China, and the etiological agent of Coronavirus Disease-2019 (COVID-19). This infection spreads mainly through direct contact with Flügge micro droplets or core droplets that remain suspended as aerosol. Moreover, it has been reported that infected subjects, both with and without clinical signs of COVID-19, can transmit the virus. Since the infection typically enters through mouth, nose, and eyes, dentistry is one of the medical practices at highest risk of infection due to the frequent production of aerosol and the constant presence of saliva. The World Health Organization (WHO) has suggested that only emergency/urgent procedures should be performed during the coronavirus outbreak. Considering the virus’ route of transmission, a specific protocol should be applied to reduce the risk of infection in addition to measures that prevent the spread of infection from a patient to another person or medical tools and equipment (cross-infection). This protocol should be implemented by modifying both patient management and clinical practice, introducing particular devices and organizational practices. This paper aims to discuss and suggest the most appropriate procedures in every aspect of dental practice to reduce infection risk.

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