NOVEL BIOINFORMATICS APPROACH DETECTS HUNDREDS OF PREVIOUSLY UNDETECTED SPLICED TRANSCRIPTS DISCOVERED FROM CAENORHABDITIS ELEGANS GENOME
<p><strong>CONTEXT:</strong> With the completion of genome sequence of several organisms including free-living soil nematode Caenorhabditis elegans, precise genome annotations of this sea of raw information are now of prime importance, as they allow the accurate definition of generic regions. Alternative splicing is seen in nearly all metazoan organisms as a means for producing functionally diverse polypeptides from a single gene. <strong>AIM:</strong> In this study, we performed a detailed and in-depth analysis of the full genomic sequence of one of the six chromosomes of C. elegans. <strong>MATERIALS AND METHODS:</strong> In this study, several bioinformatics tools including gene/exon prediction programs, ORF finders, blast analysis tools, and alignment programs were used to analyze the genes/exons encoded by chromosome 1 of C. elegans with special reference to alternatively spliced transcripts. <strong>CONCLUSION:</strong> Using these tools, we have predicted >200 new alternatively spliced hypothetical transcripts from the genes encoded by chromosome 1 in C. elegans. These new spliced transcripts were identified from unusually large untranslated (UTR) regions and large introns present at the 3’ and 5’ ends of the genes with a maximum number of transcripts predicted from 5’ UTR analysis. Further studies and subsequent confirmation of these alternatively spliced transcripts will enhance our understanding of the genome structure, expression, and in elucidating their role during the development of C. elegans.</p>