scholarly journals Antioxidant and Anti Aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid

2016 ◽  
Vol 16 (3) ◽  
pp. 88 ◽  
Author(s):  
Wahyu Widowati ◽  
Nurul Fauziah ◽  
Heddy Herdiman ◽  
Merry Afni ◽  
Ervi Afifah ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Activities ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Phytochemical Analysis ◽  
Coumaric Acid ◽  
Antioxidant Power ◽  
Modified Method ◽  
Reducing Activity ◽  
High Level

Aging is a natural process in humans as accumulation of oxygen-derived free radicals which leads to the activation of hyaluronidase, collagenase and elastase, that can further contribute to cellular and tissue damage. Bioactive compounds from plants have been used as antioxidant that might inhibit aging processes as well. This study aimed to determine antioxidant and anti aging properties of <em>Oryza sativa</em> Extract (OSE), and its compounds, vanillin and coumaric acid. The phytochemical analysis of OSE was performed with Farnsworth modified method. Antioxidant activities were performed by measurement of 2,2-diphenyl 1-pichylhydazyl (DPPH) free radical scavenger, Ferric Reducing Antioxidant Power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) reducing activity, while anti aging assay were observed through inhibitory of elastase, collagenase, and hyaluronidase activities. Phytochemical analysis showed the presence of terpenoids and saponins in high level. OSE showed lowest DPPH activity (IC<sub>50</sub> = 314.51 μg/mL) compared to vanillin (IC<sub>50</sub> = 283 μg/mL) and coumaric acid (IC<sub>50</sub> = 255.69 μg/mL). In ABTS assay, OSE resulted lowest activity(IC<sub>50</sub> = 145.67 μg/mL), compared to vanillin (IC<sub>50</sub> = 4.96 μg/mL) and coumaric acid (IC<sub>50</sub> = 1.67 μg/mL). OSE also showed the lowest FRAP-reducing activity (21.26 μM Fe(II)/μg), compared to vanillin (35.05 μM Fe(II)/μg) and coumaric acid (48.52μM Fe(II)/μg). OSE showed the lowest collagenase, elastase, and hyaluronidase inhibitory activity (IC<sub>50</sub> = 816.78,107.51, and 203.13 μg/mL), compared to vanillin (IC<sub>50</sub> = 16.27, 14.46, 45.23 μg/mL respectively) and coumaric acid (IC<sub>50</sub> = 146.89, 25.38, 8.21 μg/mL respectively). In summary, OSE possess the lowest antioxidant and anti aging activities compared to vanillin and coumaric acid.

Ceria-Zirconia Antioxidant Nanoparticles Attenuate Hypoxia-Induced Acute Kidney Injury by Restoring Autophagy Flux and Alleviating Mitochondrial Damage

2020 ◽  
Vol 16 (7) ◽  
pp. 1144-1159
Author(s):  
Sang-Eun Hong ◽  
Jong Hun An ◽  
Seong-Lan Yu ◽  
Jaeku Kang ◽  
Chang Gyo Park ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Kidney Injury ◽  
Mitochondrial Damage ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Autophagy Flux ◽  
Novel Approach

Oxidative stress is one of the principal causes of hypoxia-induced kidney injury. The ceria nanoparticle (CNP) is known to exhibit free radical scavenger and catalytic activities. When zirconia is attached to CNPs (CZNPs), the ceria atom tends to remain in a Ce3+ form and its efficacy as a free radical scavenger thus increases. We determined the effectiveness of CNP and CZNP antioxidant activities against hypoxia-induced acute kidney injury (AKI) and observed that these nanoparticles suppress the apoptosis of hypoxic HK-2 cells by restoring autophagy flux and alleviating mitochondrial damage. In vivo experiments revealed that CZNPs effectively attenuate hypoxia-induced AKI by preserving renal structures and glomerulus function. These nanoparticles can successfully diffuse into HK-2 cells and effectively counteract reactive oxygen species (ROS) to block hypoxia-induced AKI. This suggests that these particles represent a novel approach to controlling this condition.

scholarly journals Comparative Phytochemical Analysis and Antioxidant Activities of Tamalakyadi Decoction with Its Modified Dosage Forms

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Jeevani Maheshika Dahanayake ◽  
Pathirage Kamal Perera ◽  
Priyadarshani Galappatty ◽  
Hettiarachchige Dona Sachindra Melshandi Perera ◽  
Liyanage Dona Ashanthi Menuka Arawwawala
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dosage Forms ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Freeze Dried ◽  
Fingerprint Pattern ◽  
Spray Dried

Background and Objective. Tamalakyadi decoction (TD) is a classical formulation mentioned in authentic traditional medicine text Sarasankshepaya under nasal diseases and used as a remedy for allergic rhinitis. It consists of 12 plant ingredients. Decoction preparations are widely used in Sri Lankan traditional system and considered effective and safe for treating many disorders. However, decoctions have to be used only in fresh state due to shorter shelf life and loss of stability. This decoction preparation method leads to decreasing the patient compliance and is also time consuming. Hence, the objective of the present study was to convert TD to consumer friendly novel dosage form, namely, freeze dried, spray dried, and traditional ganasara forms. Methodology. Therefore, we compared the phytochemical constituents and antioxidant activities of TD with its modified dosage forms. The chemical comparison of four dosage forms comprises phytochemical screening, TLC and HPTLC fingerprint profiles and the antioxidant activities by DPPH free radical scavenging activity, Ferric reducing antioxidant power (FRAP), total polyphenol content (TPC), and total flavonoid content (TFC). Results. Phytochemical screening revealed the presence of alkaloids, saponins, tannins, steroids, flavonoids, phenols, and terpenoids in all dosage forms. However, the saponins, alkaloids, flavonoids, terpenoids, and steroids were more prominent in TD and freeze dried preparation than the other two preparations. HPTLC fingerprint pattern of freeze dried dosage was more similar with HPTLC fingerprint pattern of TD in terms of number of peaks and their intensity compared to that of spray dried and ganasara dosage forms. Antioxidant activities such as DPPH, FRAP, TPC, and TFC were higher in decoction and freeze dried preparation than in spray dried and ganasara preparation. Conclusion. Freeze dried TD is the most suitable ready to use preparation having similar chemical properties and antioxidant activities to TD.

Elaeocarpus floribundus Bl. seeds as a new source of bioactive compounds with promising antioxidant and antimicrobial properties

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ayorinde V. Ogundele ◽  
Saikat Haldar ◽  
Archana Yadav ◽  
Archana M. Das
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Medicinal Uses ◽  
Wide Range

AbstractThe fruits of Elaeocarpus floribundus Bl. (Elaeocarpaceae) are edible and are normally prescribed for treatment of diseases. The medicinal uses of the fruit create considerable quantities of seeds as wastes. In an attempt to valorise this biomass, we studied the antimicrobial and antioxidant activities of the ethanolic seed extract. The extract inhibited the growth of the tested pathogens and was also a very strong scavenger of DPPH free radicals. Consequently, the extract was phytochemically investigated and this study reports the initial isolation of five phenolic compounds from this source. The structures of the isolated compounds were elucidated by spectra analyses including HR-ESI-MS, 1D and 2D NMR experiments. The isolated compounds exhibited a wide range of antimicrobial activities against the tested pathogens. Gallic acid (4) showed the most activity against Bacillus subtilis with a minimum inhibitory concentration (MIC) value of 30 µg/mL, while the MIC values of the antimicrobial standards range between 10 and 35 µg/mL. Compound 4, crude ethanolic extract, and the ethyl acetate fraction were more a potent free radical scavenger of DPPH compared to ascorbic acid. Hence, the seeds of E. floribundus could be considered as a new source of bioactive compounds for pharmaceutical and food-related industries.

scholarly journals Reviews on Mechanisms ofIn VitroAntioxidant Activity of Polysaccharides

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Junqiao Wang ◽  
Shuzhen Hu ◽  
Shaoping Nie ◽  
Qiang Yu ◽  
Mingyong Xie
Keyword(s):  
Antioxidant Activities ◽  
Cell Structure ◽  
Free Radical Scavenger ◽  
Antioxidant Systems ◽  
Radical Scavenger ◽  
Significant Damage ◽  
Nonenzymatic Antioxidants ◽  
Underlying Mechanisms

It is widely acknowledged that the excessive reactive oxygen species (ROS) or reactive nitrogen species (RNS) induced oxidative stress will cause significant damage to cell structure and biomolecular function, directly or indirectly leading to a number of diseases. The overproduction of ROS/RNS will be balanced by nonenzymatic antioxidants and antioxidant enzymes. Polysaccharide or glycoconjugates derived from natural products are of considerable interest from the viewpoint of potentin vivoandin vitroantioxidant activities recently. Particularly, with regard to thein vitroantioxidant systems, polysaccharides are considered as effective free radical scavenger, reducing agent, and ferrous chelator in most of the reports. However, the underlying mechanisms of these antioxidant actions have not been illustrated systematically and sometimes controversial results appeared among various literatures. To address this issue, we summarized the latest discoveries and advancements in the study of antioxidative polysaccharides and gave a detailed description of the possible mechanisms.

scholarly journals Effect ofBetula pendulaLeaf Extract onα-Glucosidase and Glutathione Level in Glucose-Induced Oxidative Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Kristina Bljajić ◽  
Nina Šoštarić ◽  
Roberta Petlevski ◽  
Lovorka Vujić ◽  
Andrea Brajković ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Metal Ion ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Radical Scavenger ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Quercetin Derivatives

B. pendulaleaf is a common ingredient in traditional herbal combinations for treatment of diabetes in southeastern Europe. Present study investigatedB. pendulaethanolic and aqueous extract as inhibitors of carbohydrate hydrolyzing enzymes, as well as their ability to restore glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress. Phytochemical analysis revealed presence of rutin and other quercetin derivatives, as well as chlorogenic acid. In general, ethanolic extract was richer in phenolic substances than the aqueous extract. Furthermore, a comprehensive analysis of antioxidant activity of two extracts (determined by DPPH and ABTS radical scavenging activity, total antioxidant activity, and chelating activity as well as ferric-reducing antioxidant power) has shown that ethanolic extract was better radical scavenger and metal ion reductant. In addition, ethanolic extract effectively increased cellular glutathione levels caused by hyperglycemia and inhibitedα-glucosidase with the activity comparable to that of acarbose. Therefore,in vitroresearch usingB. pendulaplant extracts has confirmed their antidiabetic properties.

scholarly journals Phytochemistry, Antioxidant Activity and Heavy Metal Content on Precooked and Raw of Celosia argentea L., Launaea taraxacifolia Wild. and Ocimum gratissimum L., Three Leafy Vegetables Eaten in Southern Benin

2019 ◽  
Vol 8 (1) ◽  
pp. 71
Author(s):  
Kizito Thimoléon AGBESSY ◽  
Camel Lagnika ◽  
Abdou Madjid O. Amoussa ◽  
John Nsor-Atindana ◽  
Ambaliou SANNI ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Leafy Vegetables ◽  
World Health ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Ocimum Gratissimum ◽  
Celosia Argentea

This study aimed to estimate and compare the antioxidant potential as well as the levels of heavy metal contamination of Celosia argentea (Amaranthaceae), Launaea taraxacifolia (Asteracea) and Ocimum gratissimum (Lamiaceae) collected in four municipalities of South-Benin by taking into accounts the treatment prior to consumption (precooked and raw). A low extraction yield was obtained during the extraction of leafy vegetables subjected to precooking. The phytochemical screening of Launaea taraxacifolia revealed the presence of triterpenes, steroids, flavonoids and tannins. The phytochemical analysis of Ocimum gratissimum revealed the presence of triterpenes, the steroids, tannins, anthraquinones, coumarins and alkaloids. The analysis of Celosia argentea revealed the presence of triterpenes, steroids, flavonoids, tannins, anthraquinones and alkaloids. The antioxidant activity of the various extracts was found to be dose-dependent. The extracts showed significant antioxidant activities with IC50 ranging from 4.03 to 11.42 µg/ml. Ocimum gratissimum had the strongest antioxidant power followed by Celosia argentea and Launaea taraxacifolia respectively. Pre-cooking of leafy vegetables would have an effect on antioxidant activity, which resulted in a slight increase in the DPPH inhibition power of pre-cooked vegetables compared to raw one. The analysis of heavy metals showed the presence of arsenic, mercury and manganese in the different plant extracts. The highest levels were observed in leafy vegetables collect at Cotonou (Houeyiho) followed by those from Sèmè-Podji but all below the thresholds defined by the World Health Organization. The precooking promoted a decrease of the quantity in heavy metals by leaching, causing low concentration in metals of vegetables precooked in contrast with raw ones.

scholarly journals Phytochemical analysis and antimicrobial and antioxidant activities of Celosia argentea leaves

2021 ◽  
Vol 17 (9) ◽  
Author(s):  
Genésio José da Silva Neto ◽  
Eronildo Joaquim de Santana ◽  
Amanda Reges de Sena ◽  
Bruno Anderson de Morais ◽  
Maria Isabela Ferreira de Araújo ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Reducing Power ◽  
Spectrophotometric Analysis ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Diffusion Technique ◽  
Celosia Argentea ◽  
Total Antioxidant ◽  
Layer Chromatography

This study performed the phytochemical screening and bioactivity of Celosia argentea leaves. The phytochemical profile of hexane (Hex), ethyl acetate (AcOEt) and methanolic (MeOH) extracts of C. argentea leaves was observed by Thin Layer Chromatography and spectrophotometric analysis. In the susceptibility test, the disk-diffusion technique was performed. Minimum Inhibitory Concentration (MIC) was assessed by microdilution. Minimum Microbic Concentration (MMC) was determined in the extracts which presented MIC. Antioxidant activities were measured using 2,2-diphenyl-1-picril-hidrazil (DPPH), determination of reducing power and total antioxidant capacity (TAC). The results showed a higher content of phenolic compounds (252.02 ± 0.02 mg GAE/g) in the extract AcOEt, and of tannins (103.72 ± 0.004 mg/EAT/g) in MeOH; this was effective against Micrococcus luteus (inhibition of 24.7 ± 0.6 mm). MIC and CMM of the AcOEt for M. luteus were 0.06 and 0.25 mg/mL, respectively. The MeOH extract eliminated 70.4 ± 0.03% of DPPH radical, whereas AcOEt had greater reducing power (289.23 ± 0.05 mg AA/g) and TAC at a concentration of 250 µg/mL (89.6%). This study revealed that C. argentea has phytochemicals with bactericidal and fungicidal potential, in addition to elevated antioxidant power. Key words: antimicrobial, antioxidant, bioactivity.

scholarly journals Enhancement of Antioxidant and Isoflavones Concentration in Gamma Irradiated Soybean

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Boris M. Popović ◽  
Dubravka Štajner ◽  
Anamarija Mandić ◽  
Jasna Čanadanović-Brunet ◽  
Slavko Kevrešan
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenol Content ◽  
Radical Scavenger ◽  
Multivariate Techniques ◽  
Soybean Genotype ◽  
Antioxidant Power ◽  
Scavenging Effect ◽  
Ferric Reducing Antioxidant Power ◽  
Gamma Irradiated

Serbian soybean genotype Ana was gamma irradiated at doses of 1, 2, 4, and 10 kGy in order to evaluate the influence of gamma irradiation on isoflavone (genistein, daidzein, and their glycosides genistin and daidzin) contents and hydroxyl radical scavenging effect (HRSE). The increase in genistin and daidzin contents as well as antioxidant activities was observed especially at doses of 4 and 10 kGy. Results were also compared with our previous results relating to total phenol content (TPC), DPPH radical scavenger capacity (DPPH RSC), and ferric reducing antioxidant power (FRAP). Our results indicated that doses up to 10 kGy improve the antioxidant activities of soybean and also nutritional quality with respect to isoflavone content. All results were analyzed by multivariate techniques (correlation matrix calculation and autoscaling transformation of data). Significant positive correlations were observed between genistin, daidzin, DPPH RSC, and HRSE.

scholarly journals Administration Dependent Antioxidant Effect ofCarica papayaSeeds Water Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Elisa Panzarini ◽  
Majdi Dwikat ◽  
Stefania Mariano ◽  
Cristian Vergallo ◽  
Luciana Dini
Keyword(s):  
Oxidative Stress ◽  
Carica Papaya ◽  
Transmembrane Potential ◽  
Herbal Remedy ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Water Extract ◽  
Free Radical Scavenger ◽  
Antioxidant Effect ◽  
Radical Scavenger

Carica papayais widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of theC. papayaseeds water extract against hydrogen peroxide (H2O2) oxidative stress in human skin Detroit 550 fibroblasts.C. papayaseeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2oxidative stress. Data show that (i) the maximum protective effect is achieved by the simultaneous administration of the extract with 1 mM H2O2; (ii) the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential(Δψm)loss; (iii) the extract is more efficient than vitamin C to hamper the oxidative damage; (iv) the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion,C. papayaseeds water extract is potentially useful for protection against oxidative stress.

Thermal and acido-basic stability of antioxidant properties of extracts from cereal and pseudocereal grains

Biologia ◽  
2013 ◽  
Vol 68 (1) ◽  
Author(s):  
Tibor Maliar ◽  
Mária Maliarová ◽  
Ján Kraic ◽  
Miroslav Ondrejovič ◽  
Ivana Pšenáková ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Raw Material ◽  
Antioxidant Power ◽  
Beneficial Effects ◽  
Barley Cultivars ◽  
Ferric Reducing Antioxidant Power ◽  
The Stability ◽  
High Level

AbstractBeneficial effects of whole grains of cereals and pseudocereals and their fractions to human physiology are well known and broadly published. Especially secondary metabolites, dominantly from the category of phenolics (or polyphenols), beneficially influence the health physiology and/or prevent disease progress. Within the frame of this study, ten genotypes of four cereals or pseudocereals, respectively, were chosen for their antioxidant activity, determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and β-carotene-linoleic acid bleaching model (BCLM) mechanisms. Tested genotypes were selected from primary collection based on their antioxidant activity values, as well as higher level of flavonoids or phenolic acids. The stability of antioxidant properties after thermic, acidic, and basic treatments was evaluated. The oat cultivar Sirene and buckwheat cultivar Bogatyr expressed high level of the antioxidant activity, but they lost it due to all types of treatment. Oppositely, treatments increased antioxidant activities in some samples, especially in oat cultivar Maris Oberon, wheat cultivar Ines and Karolinum, or partially in barley cultivars Kompakt (after basic treatment) and Jubilant (acidic and basic treatments). The lack of the antioxidant activity could be observed due to destruction of the key compounds responsible for the antioxidant effect, whereas the increasing activity could be seen due to release of the aglycons from glycosidic forms after treatment. The stability of antioxidant properties could be a valuable parameter of the raw material for manufacturing special foods with functional properties.

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