scholarly journals PATHOLOGICAL CHANGES OF PERIODONTAL TISSUES IN CONDITIONS OF EXPERIMENTAL MODELING OF PARODONTITIS IN RABBITS

2018 ◽  
Vol 14 (4) ◽  
pp. 50-53
Author(s):  
Виктория Мирошниченко ◽  
Viktoriya Miroshnichenko ◽  
Вадим Марчук ◽  
Vadim Marchuk ◽  
Павел Четвертак ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Biological Model ◽  
Pathological Changes ◽  
Biopsy Material ◽  
Tissue Samples ◽  
Periodontal Tissues ◽  
Lower Jaw ◽  
Histological Data ◽  
Clinical Changes ◽  
Marginal Division

Background. Developed local periodontitis in a laboratory rabbit was reproduced experimentally with a ligature method used. Clinical changes, peculiarities of anatomical structure and pathomorphological changes of periodontal tissues were considered at the stages of the experiment. Perfected the technique of local initiation of periodontitis around the incisors of the lower jaw was perfected with the help of ligature method by Volozhin A. I., Vinogradova S. I. modification of 1993. The features of the technique and recommendations for its implementation were stated. The possibility of using rabbits as a biological model for modeling inflammation in periodontal tissues was evaluated. Object. The aim of the study was to assess in normal conditions and to compare the pathological changes of marginal division of the gums on the stages of artificially initiated periodontitis in the rabbit with the data obtained biopsy material in humans. Materials and methods. The experiment was conducted on three rabbits of the Chinchilla breed weighing 2500 to 3000 grams, in which local periodontitis initiated according to the method of initiation of local periodontitis around the incisors of the lower jaw with ligature method by Volozhin A. I., Vinogradova S. I. modification of 1993. Clinical and histological data were collected during the experimental stages. Then they were compared with the data obtained in humans. Results. The study has identified features of technology for artificial simulation of periodontitis. Also, it has been determined that the histological tissue samples in norm and pathology, and clinical signs of developed periodontitis proved similarity of anatomical and pathological structures at marginal division of the gum for both rabbits and humans. Thus, the use of rabbits as a biological model for the modeling of periodontitis and the choice of modern methods of treatment of periodontitis is possible.

Histopathologic lesions in conventional pigs experimentally infected with Haemophilus parasuis serovar 5

2015 ◽  
Vol 43 (02) ◽  
pp. 91-96 ◽  
Author(s):  
R.-L. Austin-Busse ◽  
A. Ladinig ◽  
G. Balka ◽  
S. Zoels ◽  
M. Ritzmann ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Control Group ◽  
Post Inoculation ◽  
Haemophilus Parasuis ◽  
Tissue Samples ◽  
Group A ◽  
Pathologic Lesions ◽  
Group B ◽  
Kidney Lesions ◽  
Challenge Group

Summary Objective: In the present study various tissues of pigs were investigated for the presence of histopathologic lesions after an experimental infection with Haemophilus (H.) parasuis serovar 5. Material and methods: Conventional pigs (n = 36) were divided into a control group B (n = 9) and a challenge group A (n = 27), which was infected intratracheally. Pigs that did not die prior to study termination were euthanized on day 14 post inoculation. Postmortem samples of the lung, heart, liver, kidney, spleen, left tarsal joint capsule and brain were collected. Results: All but one pig with detectable histopathologic lesions (n = 11) showed typical macroscopic changes. Histopatho logic examination of all tissue samples identified pyelitis (n = 10), synovitis (n = 7) and meningitis (n = 7) and all those animals were euthanized prior to study termination. No histopathologic lesions were found in pigs of the control group. The correlations between pyelitis and meningitis, pyelitis and synovitis and synovitis and meningitis were significant (p < 0.001). No significant correlation could be observed between the histopathologic and the clinical examination of the joints. The investigation of samples from the joints by PCR was not significantly correlated with the observed synovitis. The clinical observation of neurologic signs was significantly correlated with meningitis (p = 0.03). A significant correlation (p < 0.001) could be detected between meningitis and the detection of H. parasuis by PCR in brain samples. Conclusions: H. parasuis constantly causes clinical signs and pathologic lesions as soon as it infects the brain while it can infect the joints without causing histopathologic lesions. Pigs with histopathologic lesions do not always show typical clinical signs. Only few studies described the finding of kidney lesions in pigs with Glässer’s disease and this is the first study to describe a pyelitis in pigs experimentally infected with H. parasuis. The observed pyelitis mainly occurred in acute cases.

scholarly journals Pathogenicity of an African swine fever virus strain isolated in Vietnam and alternative diagnostic specimens for early detection of viral infection

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hu Suk Lee ◽  
Vuong Nghia Bui ◽  
Duy Tung Dao ◽  
Ngoc Anh Bui ◽  
Thanh Duy Le ◽  
...  
Keyword(s):  
Early Detection ◽  
Oral Fluid ◽  
African Swine Fever Virus ◽  
Fluid Collection ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Post Inoculation ◽  
Tissue Samples ◽  
Viral Loads ◽  
Specimen Collection

Abstract Background African swine fever (ASF), caused by the ASF virus (ASFV), was first reported in Vietnam in 2019 and spread rapidly thereafter. Better insights into ASFV characteristics and early detection by surveillance could help control its spread. However, the pathogenicity and methods for early detection of ASFV isolates from Vietnam have not been established. Therefore, we investigated the pathogenicity of ASFV and explored alternative sampling methods for early detection. Results Ten pigs were intramuscularly inoculated with an ASFV strain from Vietnam (titer, 103.5 HAD50/mL), and their temperature, clinical signs, and virus excretion patterns were recorded. In addition, herd and environmental samples were collected daily. The pigs died 5–8 days-post-inoculation (dpi), and the incubation period was 3.7 ± 0.5 dpi. ASFV genome was first detected in the blood (2.2 ± 0.8) and then in rectal (3.1 ± 0.7), nasal (3.2 ± 0.4), and oral (3.6 ± 0.7 dpi) swab samples. ASFV was detected in oral fluid samples collected using a chewed rope from 3 dpi. The liver showed the highest viral loads, and ear tissue also exhibited high viral loads among 11 tissues obtained from dead pigs. Overall, ASFV from Vietnam was classified as peracute to acute form. The rope-based oral fluid collection method could be useful for early ASFV detection and allows successful ASF surveillance in large pig farms. Furthermore, ear tissue samples might be a simple alternative specimen for diagnosing ASF infection in dead pigs. Conclusions Our data provide valuable insights into the characteristics of a typical ASFV strain isolated in Vietnam and suggest an alternative, non-invasive specimen collection strategy for early detection.

scholarly journals Two Live Attenuated Vaccines against Recent Low–and Highly Pathogenic H7N9 Influenza Viruses Are Safe and Immunogenic in Ferrets

Vaccines ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 74 ◽  
Author(s):  
Larisa Rudenko ◽  
Irina Kiseleva ◽  
Elena Krutikova ◽  
Ekaterina Stepanova ◽  
Irina Isakova-Sivak ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Phase I ◽  
Reverse Genetics ◽  
Vaccine Candidate ◽  
Clinical Signs ◽  
Influenza Viruses ◽  
Phase I Clinical Trial ◽  
World Health ◽  
Histological Data ◽  
Health Organization

Influenza H7N9 virus is a potentially pandemic subtype to which most people are immunologically naïve. To be better prepared for the potential occurrence of an H7N9 pandemic, in 2017 the World Health Organization recommended developing candidate vaccine viruses from two new H7N9 viruses, A/Guangdong/17SF003/2016 (A/GD) and A/Hong Kong/125/2017 (A/HK). This report describes the development of live attenuated influenza vaccine (LAIV) candidates against A/GD and A/HK viruses and study of their safety and immunogenicity in the ferret model in order to choose the most promising one for a phase I clinical trial. The A/HK-based vaccine candidate (A/17/HK) was developed by classical reassortment in eggs. The A/GD-based vaccine candidate (A/17/GD) was generated by reverse genetics. Ferrets were vaccinated with two doses of LAIV or phosphate-buffered saline. Both H7N9 LAIVs tested were safe for ferrets, as shown by absence of clinical signs, and by virological and histological data; they were immunogenic after a single vaccination. These results provide a compelling argument for further testing of these vaccines in volunteers. Since the A/HK virus represents the cluster that has caused the majority of human cases, and because the A/HK-based LAIV candidate was developed by classical reassortment, this is the preferred candidate for a phase I clinical trial.

scholarly journals Clinical, pathological and epidemiological aspects of outbreaks of bluetongue disease in sheep in the central region of Rio Grande do Sul

2017 ◽  
Vol 37 (12) ◽  
pp. 1443-1452
Author(s):  
Ronaldo M. Bianchi ◽  
Welden Panziera ◽  
Tatiane C. Faccin ◽  
Gisane L. de Almeida ◽  
Juliana F. Cargnelutti ◽  
...  
Keyword(s):  
Central Region ◽  
Striated Muscle ◽  
Clinical Signs ◽  
Rio Grande ◽  
Nasal Discharge ◽  
Rio Grande Do Sul ◽  
Sample Collection ◽  
Area At Risk ◽  
Tissue Samples ◽  
Facial Swelling

ABSTRACT: This article describes the clinical, pathological and epidemiological aspects of 17 outbreaks of bluetongue (BT) disease in sheep occurring between December 2014 and July 2015 in the central region of Rio Grande do Sul state (RS), southern Brazil. Affected farms were visited for clinical examination, necropsy, sample collection and epidemiological investigation. The outbreaks were seasonal and occurred during the summer and autumn. A total of 180 sheep (20.4%) out of 884 in 17 small herds were affected. All ages of Texel and mixed breed sheep were affected. However, lambs (younger than one year) had higher morbidity than adult sheep. The most frequent clinical signs were anorexia, lethargy, loss of body condition, facial swelling mainly involving the lips, and greenish seromucous or mucous nasal discharge. Pulmonary lesions characterized by edema were the most prevalent findings; however, erosive and ulcerative lesions in the upper gastrointestinal tract, as well as cardiac, skeletal muscle and esophageal striated muscle necrosis, and hemorrhage in the pulmonary artery were also frequent. The bluetongue virus (BTV) genome was detected by RT-PCR in blood and tissue samples (spleen and lungs) of 21 animals from 17 outbreaks. The virus involved in the outbreak 3 was subsequently isolated and shown to belong to serotype 17, for the first time reported in Brazil. In summary, our data support the BTV genotype 17 as the etiological agent of the outbreaks and indicate that the central region of RS is an area at risk for BT in sheep, a disease previously not recognized in the region.

scholarly journals Bullous Pemphigoid in a Dog

2021 ◽  
Vol 49 ◽  
Author(s):  
Suélen Dalegrave ◽  
Denner Francisco Tomadon Fiorin ◽  
Eduarda Gabriela Mansour ◽  
Monica Regina De Matos ◽  
Renato Herdina Erdmann ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Plasma Cells ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Punch Biopsy ◽  
Mucous Membrane Pemphigoid ◽  
Histopathological Analysis ◽  
Biopsy Material ◽  
Clinicopathological Findings

Background: In dogs, bullous pemphigoid (BP) is a subepithelial autoimmune disease, a rare dermatopathy in the clinical routine. BP is characterized by formation of vesicles and subepidermal blisters that result from dissolution of the dermal-epithelial junction. Clinical signs of BP usually include severe dermatological alterations with a variable prognosis. The aim of this work is to report a case of BP in a dog to contribute information for diagnosis, and to present clinical and pathological aspects that emerge during development of BP.Case: An adult male mongrel dog exhibited hyperemic, exudative, crusty lesions on the lip commissure and periocular areas. Results from laboratory tests were normal. Results from parasitological and mycological tests on skin scrapings were negative. Imprint cytology of the crusts revealed presence of gram-positive cocci bacteria. In the histopathological analysis of punch biopsy material, the epidermis was detached from the dermis, leading to formation of vesicles. There were inflammatory infiltrates containing neutrophils, eosinophils, and high amounts of fibrin, and areas of multifocal orthokeratotic hyperkeratosis. Multifocal infiltrates containing lymphocytes, histiocytes, and plasma cells were observed on the superficial portions of the dermis, which indicated a diagnosis of BP. After the definitive clinical diagnosis, the animal was treated with enrofloxacin (Baytril Flavour®; 5 mg/kg once a day for 10 days), and prednisolone (Prediderm®; 2 mg/kg once a day until further instructions). On the follow-up visit, 15 days later, the clinical picture had improved, and the lesions had decreased. Continuity of the treatment was prescribed, along with a gradual decrease in the corticoid dose. The dose of prednisolone was initially reduced to 1 mg/kg once a day, and later to 0.5 mg/kg until improvement of the clinical status of the patient. Remission of the lesions was observed 13 weeks later.Discussion: The diagnosis of BP was established after identification of the clinical cutaneous lesions and observation of microscopic findings on punch biopsy material obtained from the ocular and lip regions. BP does not exhibit breed or sex predisposition, and affects adult dogs. The clinical signs of BP are characteristic of autoimmune diseases that affect the dermoepidermal junction, and consist of erythematous, ulcerated, crusty, and painful lesions on the nose, dorsal area of the muzzle, and periorbital region. However, these lesions must be differentiated, by histological analysis, from several other conditions with a similar clinical presentation. Diseases that must be considered in the differential diagnosis comprise other variants of the pemphigus complex, lupus erythematosus, drug eruption, erythema multiforme, toxic epidermal necrolysis, epitheliotropic lymphoma, inherited bullous epidermolysis, mucous membrane pemphigoid, and lymphoreticular neoplasia. The clinicopathological findings indicated that the lesions were compatible with BP. The occurrence of necrotic and erythematous lesions is due to production of antibodies accompanied by a strong response of neutrophils, which results in loss of cell adhesion and epidermal necrosis. The presence of detachment of the epidermis from the dermis, inflammation in the superficial portion of the dermis, and infiltrates containing lymphocytes, histiocytes and plasma cells observed at the histopathological examination indicated the occurrence of BP. The skin histopathological examination warranted establishment of a diagnosis and therapeutic success. The lack of recurrence of clinical manifestations 43 weeks after the end of the glucocorticoid treatment demonstrated that the therapeutic approach and the cooperation of the owner are essential for success of the treatment.

Low sensitivity of histopathological examination of peri-implant tissue samples in diagnosing postoperative spinal implant infection

2020 ◽  
Vol 102-B (7) ◽  
pp. 899-903
Author(s):  
Justus Bürger ◽  
Yannick Palmowski ◽  
Patrick Strube ◽  
Carsten Perka ◽  
Michael Putzier ◽  
...  
Keyword(s):  
Spinal Surgery ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
Implant Infection ◽  
Tissue Samples ◽  
Spinal Implant ◽  
Cutibacterium Acnes ◽  
Microbiological Examination ◽  
Study Population ◽  
Low Sensitivity

Aims To evaluate the histopathological examination of peri-implant tissue samples as a technique in the diagnosis of postoperative spinal implant infection (PSII). Methods This was a retrospective analysis. Patients who underwent revision spinal surgery at our institution were recruited for this study. PSII was diagnosed by clinical signs, histopathology, and microbiological examination of intraoperatively collected samples. Histopathology was defined as the gold standard. The sensitivity for histopathology was calculated. A total of 47 patients with PSII and at least one microbiological and histopathological sample were included in the study. Results PSII occurred in approximately 28% of the study population. Histopathology showed a sensitivity of 51.1% in the diagnosis of PSII. The most commonly found pathogens were Cutibacterium acnes and gram-positive staphylococci. Conclusion Histopathology has low sensitivity for detecting PSII. In particular, infections caused by low-virulence microorganisms are insufficiently detected by histopathology. Cite this article: Bone Joint J 2020;102-B(7):899–903.

scholarly journals Calves Infected with Virulent and Attenuated Mycoplasma bovis Strains Have Upregulated Th17 Inflammatory and Th1 Protective Responses, Respectively

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 656 ◽  
Author(s):  
Chao ◽  
Han ◽  
Liu ◽  
Li ◽  
Peng ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Post Infection ◽  
Mononuclear Cells ◽  
Clinical Signs ◽  
Mycoplasma Bovis ◽  
Pathological Changes ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Induced Immunity

Mycoplasma bovis is a critical bovine pathogen, but its pathogenesis remains poorly understood. Here, the virulent HB0801 (P1) and attenuated HB0801-P150 (P150) strains of M. bovis were used to explore the potential pathogenesis and effect of induced immunity from calves’ differential transcriptomes post infection. Nine one-month-old male calves were infected with P1, P150, or mock-infected with medium and euthanized at 60 days post-infection. Calves in P1 group exhibited other clinical signs and pathological changes compared to the other two groups. Transcriptome profiles of peripheral blood mononuclear cells revealed seven and 10 hub differentially expressed genes (DEGs) in P1 and P150 groups compared with mock-infected group, respectively. Then, P1-induced pathogenesis was predicted to be associated with enhanced Th17, and P150-induced immunity with Th1 response and expression of ubiquitination-associated enzymes. Association analysis showed that 14 and 11 DEGs were positively and negatively correlated with pathological changes, respectively. Furthermore, up-regulated expression in molecules critical to differentiation of pathogenic Th17 cells in lung and peripheral blood mononuclear cells in P1 group was validated at RNA and protein levels. The results confirmed virulent and attenuated strains might be associated with biased differentiation of pro-inflammatory pathogenic Th17 and Th1 subsets respectively.

scholarly journals Surgical model of osteoarthritis secondary to medial patellar luxation in dogs

2011 ◽  
Vol 56 (No. 3) ◽  
pp. 123-130 ◽  
Author(s):  
M.R. Alam ◽  
H.B. Lee ◽  
M.S. Kim ◽  
N.S. Kim
Keyword(s):  
Articular Cartilage ◽  
Cruciate Ligament ◽  
Clinical Signs ◽  
Experimental Period ◽  
Control Group ◽  
Cranial Cruciate Ligament ◽  
Tissue Samples ◽  
Surgical Model ◽  
One Year ◽  
The Right

This study was performed to make a surgical model of osteoarthritis (OA) in the dog. Experimental medial patellar luxation (MPL) was surgically produced in the left stifle (index) of 24 skeletally mature mixed small breed dogs (age two to six years and weight 2.8 to 9 kg). The animals were randomly allocated in 2 groups; sham group (n = 12), where the right stifle was sham operated and control group (n = 12) with intact right stifle. Physical and radiographic examinations of both stifles were performed at 1.5 months intervals over a one-year experimental period. One dog was euthanatized every three months, and both stifles were explored, gross examination was performed and tissue samples from the articular cartilage, cranial cruciate ligament (CCL) and synovium were collected for histomorphology. The clinical signs of OA were obvious in the experimental dogs by 12 weeks of surgical induction of MPL, which was also evidenced in the histopathology of the joint tissues and electron microscopy of the articular cartilage. The radiographic changes of OA were not obvious until remarkable degenerative changes became abvious six months postoperatively. Surgically induced MPL can be a successful tool for experimental induction of OA in dogs.

scholarly journals Malignant Catarrhal Fever in a Captive American Bison (Bison Bison) in Italy

2008 ◽  
Vol 20 (6) ◽  
pp. 843-846 ◽  
Author(s):  
Marco Campolo ◽  
Maria Stella Lucente ◽  
Viviana Mari ◽  
Gabriella Elia ◽  
Antonella Tinelli ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Systemic Disease ◽  
Clinical Signs ◽  
Antibody Test ◽  
Ovis Aries ◽  
Bison Bison ◽  
Domestic Sheep ◽  
Malignant Catarrhal Fever ◽  
Tissue Samples ◽  
Acute Form

Malignant catarrhal fever (MCF) is a fatal, systemic disease of cattle and other domestic and wild ruminants that, in Europe, is caused by Ovine herpesvirus 2 (OvHV-2). American bison ( Bison bison) are highly susceptible to the disease. An adult American bison, housed in a zoo in southern Italy in close cohabitation with a group of domestic sheep ( Ovis aries aries) displayed clinical signs that resembled the acute form of MCF. By real-time polymerase chain reaction, OvHV-2 DNA was detected intravitam in blood, in nasal and ocular swabs, and postmortem in tissue samples of the bison. By indirect fluorescent antibody test, high MCF antibody titers were found in the bison serum. Ovine herpesvirus 2 DNA and antibodies were also found in blood samples from the domestic sheep, thus suggesting a potential role of these animals as a source of the infection. To the authors' knowledge, this is the first MCF case in captive ruminants in Italy and the second confirmed case in captive bison of European zoos.

scholarly journals Interobserver Agreement for Endometrial Cancer Characteristics Evaluated on Biopsy Material

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
S. Nofech-Mozes ◽  
N. Ismiil ◽  
V. Dubé ◽  
R. S. Saad ◽  
Z. Ghorab ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Paradigm Shift ◽  
Interobserver Agreement ◽  
Tumor Grade ◽  
Nuclear Grade ◽  
Risk Category ◽  
Patterns Of Failure ◽  
Biopsy Material ◽  
Tissue Samples ◽  
Selective Approach

A shift toward a disease-based therapy designed according to patterns of failure and likelihood of nodal involvement predicted by pathologic determinants has recently led to considering a selective approach to lymphadenectomy for endometrial cancer. Therefore, it became critical to examine reproducibility of diagnosing the key determinants of risk, on preoperative endometrial tissue samples as well as the concordance between preoperative and postresection specimens. Six gynaecologic pathologists assessed 105 consecutive endometrial biopsies originally reported as positive for endometrial cancer for cell type (endometrioid versus nonendometrioid), tumor grade (FIGO 3-tiered and 2-tiered), nuclear grade, and risk category (low risk defined as endometrioid histology, grade 1 + 2 and nuclear grade <3). Interrater agreement levels were substantial for identification of nonendometrioid histology (κ= 0.63; SE = 0.025), high tumor grade (κ= 0.64; SE = 0.025), and risk category (κ= 0.66; SE = 0.025). The overall agreement was fair for nuclear grade (κ= 0.21; SE = 0.025). There is agreement amongst pathologists in identifying high-risk pathologic determinants on endometrial cancer biopsies, and these highly correlate with postresection specimens. This is ascertainment prerequisite adaptation of the paradigm shift in surgical staging of patients with endometrial cancer.

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