DYNAMICS OF CLINICAL AND IMMUNOLOGICAL INDICATORS IN COMPLEX TREATMENT OF CANDIDA-ASSOCIATED PERIODONTITIS

Subject. The problem of complex treatment of candida-associated periodontitis is considered. The authors propose a two-component probiotic, which, due to antagonistic activity regarding periodontal pathogenic infection and fungi of the genus Candida, leads to the relief of the inflammatory process, positive dynamics and stabilization of immunological parameters. The aim ― to evaluate the dynamics of clinical and immunological parameters in the complex treatment of candida-associated periodontitis with an average degree of quantitative seeding of periodontal pockets using a two-component probiotic. Methodology. A clinical and laboratory examination of three groups (I, II, III) of patients with Candida-associated periodontitis with an average degree of quantitative contamination of periodontal pockets (> 3 <6 CFU / ml) was carried out. Patients I groups were prescribed the well-known complex treatment including an antimycotic drug, patients II groups included a two-component probiotic locally in the treatment complex, and patients III groups locally and per os. The state of the oral mucosa was assessed, PMA, PI, OHI - S were determined, and indicators of cellular and humoral immunity were examined. Results. The study made it possible to establish that the inclusion of a two-component probiotic in the complex of treatment of candida-associated periodontitis with an average degree of quantitative contamination of periodontal pockets with fungi of the genus Candida contributes to the relief of the inflammatory process, an increase in phagocytosis, the metabolic activity of neutrophils, the concentration of immunoglobulins A, lysozyme levels, and a decrease in the concentration of immunoglobulins. G, positive dynamics of other indicators of immunity and their stabilization. Conclusions. The inclusion of a two-component probiotic from two strains in the complex treatment of chronic hepatitis C associated with fungi of the genus Candida contributes to the rapid relief of the inflammatory process in periodontal tissues, positive dynamics and stabilization of cellular and humoral immunity parameters.

Download Full-text

Assessment of cellular immunity in oil production operators with an imbalance of lipid metabolism

Russian Journal of Occupational Health and Industrial Ecology ◽

10.31089/1026-9428-2020-60-11-717-719 ◽

2020 ◽

Vol 60 (11) ◽

pp. 717-719

Author(s):

Inga N. Alikina ◽

Olga A. Kazakova

Keyword(s):

Lipid Metabolism ◽

Humoral Immunity ◽

Cellular Immunity ◽

Comparison Group ◽

Oil Production ◽

Observation Group ◽

Immunological Parameters ◽

Production Factors ◽

Cellular And Humoral Immunity ◽

Atherosclerotic Process

Introduction. Studies indicate the high pathogenetic significance of the immune component in the development of atherosclerosis. The aim of the study is a comparative assessment of immunological parameters in workers of petrochemical production with varying degrees of imbalance in lipid metabolism and the development of the atherosclerotic process. Materials and methods. Men working at an oil production enterprise in the Perm Region were examined. The observation group consisted of oil production operators with a diagnosis of atherosclerosis, the comparison group - with dyslipidemia syndrome. To determine the parameters of lipid metabolism, the results of a biochemical blood test were used. CD-immunogram parameters were identified by flow cytometry. Specific antibodies to benzene were determined by the allergosorbent method. Results. The results of a comparative study of fat metabolism confirmed violations of the physiological ratio of lipids in the blood of oil production workers, which were expressed in a significant imbalance in the levels of lipidogram. There was an increased level of specific IgG antibodies to benzene in the observation group in relation to the comparison group. An imbalance of cellular immunity was found, which was characterized by signs of indicators activation of cellular differentiation clusters. Conclusions. Studies of immune system compartments demonstrate excessive activation of cellular and humoral immunity in oil production workers under the influence of a combination of harmful production factors. The simultaneously formed imbalance of lipid metabolism is associated with various degrees of clinical manifestation of atherosclerotic disorders, with the influence of harmful production factors, aggressiveness of cellular and humoral immunity, and smoking.

Download Full-text

Clinical and immunological aspects of the spa treatment of patients with rheumatoid arthritis

Kazan medical journal ◽

10.17816/kazmj60722 ◽

1982 ◽

Vol 63 (2) ◽

pp. 33-35

Author(s):

R. I. Trofimova

Keyword(s):

Rheumatoid Arthritis ◽

T Lymphocytes ◽

Humoral Immunity ◽

Average Degree ◽

Spa Treatment ◽

Cellular And Humoral Immunity

The parameters of cellular and humoral immunity were studied in 160 patients with rheumatoid arthritis. Immunodeficiency of T-lymphocytes was found in 41% of patients with minimal and in 67% of patients with an average degree of activity of rheumatoid arthritis, on the basis of which various complexes of spa treatment of this disease were developed.

Download Full-text

Patients with Diamond Blackfan Anaemia Have Abnormalities of Cellular and Humoral Immunity

Blood ◽

10.1182/blood.v120.21.3484.3484 ◽

2012 ◽

Vol 120 (21) ◽

pp. 3484-3484 ◽

Cited By ~ 1

Author(s):

Deena Iskander ◽

Yvonne Harrington ◽

Irene Roberts ◽

Anastasios Karadimitris ◽

Josu de la Fuente

Keyword(s):

T Lymphocytes ◽

Humoral Immunity ◽

Lymphocyte Subsets ◽

Conflicts Of Interest ◽

Bone Marrow Failure ◽

Immunological Parameters ◽

Number Of Patients ◽

Increased Risk ◽

Cellular And Humoral Immunity ◽

Diamond Blackfan Anaemia

Abstract Abstract 3484 Diamond Blackfan anaemia (DBA) is an inherited bone marrow failure syndrome characterised by anaemia, physical anomalies and an increased risk of malignancy. Although the hallmark of DBA is anaemia secondary to pure red cell aplasia, some patients exhibit additional cytopaenias, such as neutropaenia, suggestive of a more widespread defect in haemopoietic development. Aberrant immunity causing infections has been reported in a proportion of patients, but the scope and precise nature of these immunological defects are yet to be elucidated. Fifty-nine patients with clinical and laboratory features consistent with Diamond Blackfan anaemia attend the DBA clinic at St. Mary's Hospital. The median age is 8.8 years (1.1 – 40.9). Two patients presented in utero (3.4%), 36 patients (61%) in the first twelve weeks of life, 12 patients (20.3%) from 3 to 12 months, 6 patients (10.2%) 1 to 5 years, 2 patients (3.4%) 5 to 10 years and 1 patient (1.7%) later than 18 years of age. Thirty-five patients (59.3%) had systemic abnormalities [including cardiac involvement in 17 patients (28.8%)], 7 patients (11.9%) had short stature only and 17 patients (28.8%) no systemic abnormalities. Infection was implicated in the death of 2 out of 3 patients in this cohort. Immunological parameters were available for 37 of the patients (age 18 months to 40 years). A history of infections was reported in 16/37 (43.2%) patients. Three patients had at least 1 serious infective episode requiring hospital admission (Salmonella gastroenteritis, Clostridum difficile gastroenteritis and neonatal pneumonia) and 13/37 patients experienced recurrent infections (7/37 respiratory tract, 1/7 urinary tract, 2/37 otitis media and 3/37 infections affecting different systems). In 2/16 patients infections occurred during treatment with corticosteroids. Consistent lymphopenia was found in 7/37 (18.9%) patients. Specific deficiencies in lymphocyte subsets were identified by immunophenotyping as summarised below. Abnormalities in one or more subsets were identified in the 7 patients with low total lymphocyte counts and a further 7 patients with normal total lymphocytes counts. A low B lymphocyte fraction was the most commonly detected abnormality, present in 12/37 (32.4% patients). Three patients (8.1%) had at least 3 abnormalities in lymphocytes subsets. Lymphocyte subsets Number patients (%) with deficiency in absolute count Median count in patients with deficiency (cells/μL) Range of counts in patients with deficiency (cells/μL) CD45+ total lymphocytes 7 (18.9) 838 387–1447 CD3+ T lymphocytes 4 (10.8) 419 266–661 CD4+ T lymphocytes 2 (5.4) 182 133–231 CD8+ T lymphocytes 4 (10.8) 141.5 83–165 CD56+ Natural Killer cells 6 (16.2) 72 49–86 CD19+ B lymphocytes 12 (32.4) 122.5 31–191 Low levels of one or more immunoglobulin isotypes were detected in 4/34 (11.8%) patients. An additional 5/32 (15.6%) patients showed a selective deficiency in one of the four IgG subclasses. Importantly, these abnormalities were masked by normal total IgG levels. Of 30 patients who had undergone immunisation against measles, mumps and rubella, immunity to all 3 pathogens was confirmed in only 13 (43.3%) patients (as determined by positive IgG serology). We observed equivocal or negative specific IgG antibodies to measles in 13/30 (43.3%), mumps in 7/30 (23.3%) and rubella in 6/30 (20%). Haemophilus B antibody titres in 30 patients following immunisation were optimum (>1mg/L) in 14/30 (46.7%), minimally protective (>0.15 mg/L) in 11/30 (36.7%) and inadequate (<0.15 mg/L) in 5/30 (16.7%). In summary, DBA patients have defects in both cellular and humoral immunity independent of treatment with steroids, with combined abnormalities of both arms of immunity in 5/37 (13.5%) patients and defects in B cells being overall the most common abnormality. In addition, a large number of patients had subnormal responses to vaccination. These findings are likely to have a role in the increased number of infections found in DBA. Disclosures: No relevant conflicts of interest to declare.

Download Full-text

Previous Humoral Immunity to the Endemic Seasonal Alphacoronaviruses NL63 and 229E Is Associated with Worse Clinical Outcome in COVID-19 and Suggests Original Antigenic Sin

Life ◽

10.3390/life11040298 ◽

2021 ◽

Vol 11 (4) ◽

pp. 298

Author(s):

Daniele Focosi ◽

Angelo Genoni ◽

Ersilia Lucenteforte ◽

Silvia Tillati ◽

Antonio Tamborini ◽

...

Keyword(s):

Humoral Immunity ◽

Cross Reactivity ◽

Clinical Severity ◽

World Health ◽

Laboratory Findings ◽

Cellular And Humoral Immunity ◽

Rate Ratios ◽

Original Antigenic Sin

Antibody-dependent enhancement (ADE) of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) infection has been hypothesized. However, to date, there has been no in vitro or in vivo evidence supporting this. Cross-reactivity exists between SARS CoV-2 and other Coronaviridae for both cellular and humoral immunity. We show here that IgG against nucleocapsid protein of alphacoronavirus NL63 and 229E correlate with the World Health Organization’s (WHO) clinical severity score ≥ 5 (incidence rate ratios was 1.87 and 1.80, respectively, and 1.94 for the combination). These laboratory findings suggest possible ADE of SARS CoV-2 infection by previous alphacoronavirus immunity.

Download Full-text

The HSP70-fused foot-and-mouth disease epitope elicits cellular and humoral immunity and drives broad-spectrum protective efficacy

npj Vaccines ◽

10.1038/s41541-021-00304-9 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Hyundong Jo ◽

Bong Yoon Kim ◽

So Hui Park ◽

Hyun Mi Kim ◽

Sung Ho Shin ◽

...

Keyword(s):

Humoral Immunity ◽

Broad Spectrum ◽

Protective Efficacy ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Host Defenses ◽

Vaccination Site ◽

Oil Emulsion ◽

Foot And Mouth ◽

Cellular And Humoral Immunity

AbstractCurrent foot-and-mouth disease (FMD) vaccines have significant limitations, including side effects due to oil emulsions at the vaccination site, a narrow spectrum of protective efficacy, and incomplete host defenses mediated by humoral immunity alone. To overcome these limitations, new FMD vaccines must ensure improved safety with non-oil-based adjuvants, a broad spectrum of host defenses within/between serotypes, and the simultaneous induction of cellular and humoral immunity. We designed a novel, immune-potent, recombinant protein rpHSP70-AD that induces robust cellular immunity and elicits a broad spectrum of host defenses against FMD virus (FMDV) infections. We demonstrated that an oil emulsion-free vaccine containing rpHSP70-AD mediates early, mid-term, and long-term immunity and drives potent host protection against FMDV type O and A, suggesting its potential as an FMD vaccine adjuvant in mice and pigs. These results suggest a key strategy for establishing next-generation FMD vaccines, including novel adjuvants.

Download Full-text

Enhancement of cellular and humoral immunity following embryonic exposure to melatonin in turkeys (Meleagris gallopavo)

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2005.03.008 ◽

2005 ◽

Vol 143 (2) ◽

pp. 178-183 ◽

Cited By ~ 9

Author(s):

C.B. Moore ◽

T.D. Siopes

Keyword(s):

Humoral Immunity ◽

Meleagris Gallopavo ◽

Embryonic Exposure ◽

Cellular And Humoral Immunity

Download Full-text

Superior cellular and humoral immunity towards SARS-CoV-2 reference and alpha and beta VOC strains in COVID-19 convalescent as compared to the prime boost BNT162b2 vaccinated dialysis patients

Kidney International ◽

10.1016/j.kint.2021.07.006 ◽

2021 ◽

Author(s):

Arturo Blazquez-Navarro ◽

Lema Safi ◽

Toni L. Meister ◽

Constantin J. Thieme ◽

Sviatlana Kaliszczyk ◽

...

Keyword(s):

Humoral Immunity ◽

Dialysis Patients ◽

Cellular And Humoral Immunity

Download Full-text

IMMU-29. B-CELL-BASED VACCINE PRODUCES GLIOBLASTOMA-REACTIVE ANTIBODIES THAT CONTRIBUTE TO TUMOR CLEARANCE

Neuro-Oncology ◽

10.1093/neuonc/noab196.388 ◽

2021 ◽

Vol 23 (Supplement_6) ◽

pp. vi98-vi98

Author(s):

Brandyn Castro ◽

Mark Dapash ◽

David Hou ◽

Aida Rashidi ◽

Deepak Kanojia ◽

...

Keyword(s):

B Cells ◽

B Cell ◽

Humoral Immunity ◽

Ex Vivo ◽

Murine Models ◽

Effector Functions ◽

Tumor Bearing ◽

Tumor Clearance ◽

Cellular And Humoral Immunity

Abstract Glioblastomas (GBM) are characterized by a strong immunosuppressive environment, contributing to their poor prognosis and limited therapeutic response to immunotherapies. B-cells represent a unique opportunity to promote immunotherapy due to their potential to kill tumors by both cellular and humoral immunity. To generate our B-cell-based vaccine (BVax) platform, we activated 41BBL+ B cells from tumor bearing mice or GBM patient blood with BAFF, CD40, and IFNg. We have previously demonstrated that BVax potentiates radiation therapy, temozolomide and checkpoint blockade in murine models of GBM via enhancement of CD8+ T-cell based immunity. The aim of this current study is to evaluate the humoral effector functions of BVax. We examined the antibody (Ab) repertoire in vivo from serum of tumor-bearing B-cell knockout mice treated with BVax or by ex vivo stimulation of patient-derived BVax. Upon systemic administration, BVax infiltrates the tumor where it differentiates into plasmablasts. Murine BVax- and BNaive-derived serum immunoglobulin generated in vivo showed that the majority of murine BVax-derived Ab were IgG isotype, while BNaive mainly produced IgM isotype. Transfer of IgG from BVax treated mice directly into tumors of recipient animals significantly prolonged their survival, demonstrating anti-tumor cytotoxicity directly through humoral immunity. Patient-derived BVax activated ex vivo showed a plasmablast phenotype and the Ab repertoire supports the previous findings seen in our murine model. Our work suggests BVax-derived IgGs role in antibody-dependent cellular cytotoxicity and improved survival in murine models. This function, in addition to its role in cellular immunity against GBM, renders BVax a potentially effective alternative immunotherapeutic option for GBM patients.

Download Full-text

Pathogenetic features of periodontitis as complications of orthodontic treatment

Medical alphabet ◽

10.33667/2078-5631-2021-24-64-68 ◽

2021 ◽

pp. 64-68

Author(s):

A. К. Sheregov ◽

М. S. Mustafayev ◽

Z. F. Kharaeva

Keyword(s):

Orthodontic Treatment ◽

The State ◽

Complex Treatment ◽

Periodontal Tissues ◽

Different Levels

The article presents the results of clinical and microbiological studies and immunological features of the state of periodontal tissues in patients with moderate periodontitis, which developed as a complication of orthodontic treatment. It was found that at the later stages of orthodontic treatment with the development of periodontitis, a complex of persistent changes in the periodontal tissues at different levels of pathogen-esis was revealed in patients. In this connection, in order to plan the complex treatment of patients with periodontitis, which has developed as a complication of orthodontic treatment, and to predict the course of the disease, it is necessary to influence the established and mutually supportive links and indicators of pathogenesis.

Download Full-text