scholarly journals FEATURES OF IMMUNOLOGICAL REACTIVITY OF PATIENTS WITH LICHEN PLANUS OF THE ORAL MUCOSA

2021 ◽  
Vol 17 (3) ◽  
pp. 24-28
Author(s):  
Vladimir Bikmurzin ◽  
Ludmila Balyabina ◽  
Irina Gimraniva ◽  
Guzel' Akmalova ◽  
Sergey Chuykin ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Dental Materials ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Immune Reactivity ◽  
Immunological Reactivity ◽  
Clinical Forms

Lichen planus is considered as a multifactorial disease. Various factors play a role in the development of lichen planus of the oral mucosa: genetic, dental materials that are used for restoration, prosthetics of teeth, Helicobacter pylori, viruses, psychological stress, somatic pathology and others. The pathogenesis of oral lichen planus is based on an autoimmune process. The aim of the study was to determine the immunological reactivity in patients with various clinical forms of lichen planus of the oral mucosa. The study carried out clinical, laboratory examination of 75 female patients aged 29 to 75 years old with red lichen planus of the oral mucosa. All patients were divided into three groups depending on the clinical form of the disease. The control group consisted of 30 women aged 39 to 70 years old without red lichen planus of the oral mucosa. The phagocytic activity of neutrophils, the level of immunoglobulins of classes A, M, G and circulating immune complexes in the blood serum were evaluated. The humoral link of immunity in patients with exudative-hyperemic form of OLP was characterized by hyperimmunoglobulinemia of classes A, M, G, an increase in circulating immune complexes, in patients with erosive-ulcerative form of hyperimmunoglobulinemia of class M against the background of hypoimmunoglobulinemia A, G and an increase in circulating immune complexes. In patients with a typical form of OLP, only an increase in the level of circulating immune complexes was noted. When assessing the immune reactivity in patients with OLP, the relationship between the manifestations of the "dysphagocytosis" syndrome, disorders in the humoral immunity system with various clinical forms of the disease was established. This fact allows us to conclude about the pathogenetic role of an imbalance in the system of mechanisms that ensure the elimination of pathogens, including infectious nature in the occurrence of a disease with the formation of an autoimmune component.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

scholarly journals Method for the determination of immune complexes by reaction with polyethylene glycol

1982 ◽  
Vol 63 (2) ◽  
pp. 10-13
Author(s):  
B. A. Molotilov ◽  
A. N. Mayansky ◽  
N. D. Pozdnyak ◽  
L. Ch. Samerkhanova
Keyword(s):  
Polyethylene Glycol ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Circulating Immune Complexes ◽  
Healthy Donors ◽  
Highly Sensitive ◽  
Photoelectric Colorimeter ◽  
Health And Disease

A study of circulating immune complexes was carried out using a reaction with polyethylene glycol. The method turned out to be simple, highly sensitive and affordable for any clinical laboratory with a photoelectric colorimeter. Analysis of the survey data of 115 healthy donors, 63 patients with rheumatoid arthritis and 16 patients with systemic lupus erythematosus made it possible to establish the level of circulating immune complexes in health and disease. The circulating immune complexes were studied in patients with rheumatism and chronic tonsillitis. To assess the results of the reaction, human aggregated gamma globulin (manufactured by Kazan NIIEM) was used.

scholarly journals The effect of interferons-α and –γ and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions

2019 ◽  
pp. 53-57
Author(s):  
A. G. Shakhov ◽  
I. T. Shaposhnikov ◽  
Yu. N. Brigadirov ◽  
V. N. Kotsarev ◽  
V. N. Skorikov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Functional State ◽  
Reproductive System ◽  
Immune Complexes ◽  
Immune Status ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Ecological Conditions ◽  
Positive Effect

The results of studying the effect of interferons alpha and gamma and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions are presented. The positive effect of the drugs, especially their combination, on erythropoiesis and the immune status of animals, which was manifested by an increase in the content of erythrocytes, hemoglobin, hematocrit, leukocytes, total immunoglobulins, serum bactericidal and lysozyme activity, absorptive function of phagocytes, relative amount of T lymphocytes, and a decrease in the level of circulating immune complexes was stated. An increase in the immune status of animals at the effect of immunocorrecting drugs was accompanied by a decrease in the pathology of calving and postpartum complications and favorably affected the functional state of their reproductive system. The cows which were introduced interferons and their combination with dimethyl dipyrazolyl selenide demonstrated a shorter period from calving to successful insemination, as well as the insemination index and fertility, compared with the animals of the control group, which were not introduced any drugs.

scholarly journals Clinical and immunological features of opisthorchiasis

2021 ◽  
Vol 102 (5) ◽  
pp. 626-635
Author(s):  
A U Sabitov ◽  
D A Soldatov ◽  
Yu B Khamanova
Keyword(s):  
Immune System ◽  
Immune Complexes ◽  
Statistical Significance ◽  
Rank Correlation ◽  
Statistical Processing ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Cytotoxic Lymphocytes ◽  
Spearman’S Rank Correlation ◽  
Diagnostic Center

Aim. To assess the clinical and immunological features in patients with chronic opisthorchiasis, depending on the duration of the infection. Methods. The first group consisted of 19 patients with the duration of the infection up to 1 year, the second group consisted of 21 patients with the duration of the infection between 1 and 5 years, the third group was formed of 23 patients with the duration of the disease more than 5 years, the control group 20 healthy individuals. Immunological research was carried out at the Clinical Diagnostic Center. Statistical processing was performed using Microsoft Excel 2010 and Statistica 6.0 software. The statistical significance of differences was determined by using the MannWhitney test (U-test) at the level of significance of p 0.05. The correlations were assessed by calculating Spearman's rank correlation coefficients.Results Clinical features of chronic opisthorchiasis were revealed in the disease duration groups of up to 1 year, from 1 year to 5 years, more than 5 years: the subclinical course was most common in the group of up to 1 year; cholangiohepatitis prevailed in the group of between 1 to 5 years, allergic skin syndrome, cholangiocholecystitis and pancreatitis dominated in the group of more than 5 years. The immune response in chronic opisthorchiasis was characterized by: up to 1 year lymphocytosis, increased levels of immunoglobulins M (IgM) and circulating immune complexes (CIC), a decrease in the number of T-lymphocytes (CD3+), as well as an increase in bactericidal activity of leukocytes (BAL); between 1 and 5 years monocytosis, increased levels of immunoglobulins M, immunoglobulins G and circulating immune complexes, a decrease in T-cytotoxic lymphocytes (CD8+) and nitro blue tetrazolium (NBT test), as well as an increase in NK cells and phagocytic activity of monocytes, more than 5 years eosinophilia. Conclusion. Common features of rearrangement of the immune system in opisthorchiasis: inflammatory changes in the hemogram, activation of humoral immunity with parallel suppression of the cellular component of the immune system, and increased phagocytosis.

scholarly journals The characteristics of serum circulating immune complexes of patients with atopic asthma with different severity degree

2013 ◽  
Vol 94 (5) ◽  
pp. 744-748 ◽  
Author(s):  
Y V Skibo ◽  
N S Kurmaeva ◽  
V N Tsibulkina ◽  
I G Mustafin ◽  
Z I Abramova
Keyword(s):  
Severe Asthma ◽  
Bronchial Asthma ◽  
Immune Complexes ◽  
Wave Length ◽  
Molecular Complexes ◽  
Electrophoretic Analysis ◽  
Circulating Immune Complexes ◽  
Atopic Asthma ◽  
Control Group ◽  
Serum Samples

Aim. To evaluate the serum level of pathogenic circulating immune complexes in patients with mild and severe atopic bronchial asthma. Methods. Serum samples of patients with atopic asthma of mild persistent (30 patients) and severe persistent (20 patients) forms were analyzed. The control group consisted of 15 healthy volunteers. To detect the giant, large, medium and small-sized serum immune complexes, 3, 3.5, 4 and 7% polyethyleneglycol-6000 solutions were used. For quantitative evaluation of the immune complexes we measured the ultraviolet optical density at 280 nm wave length. To separate the immune complexes from immunoglobulin, Protein-G-Sepharose was used. Determination of the protein composition of circulating immune complexes was performed by electrophoresis in 8% polyacrylamide gel. Results. The concentration of immune complexes was increased in patients with bronchial asthma compared to healthy donors. Small and medium-sized immune complexes were prevailing, their concentrations correlated with the severity of asthma. Large, medium and small-sized immune complexes participated in immunopathological reactions in patients with both mild and severe asthma, with immune complexes pathogenicity coefficient significantly increased depending on the severity of the disease. Electrophoretic analysis of circulating immune complexes has shown the presence of proteins with molecular weight of 60 kDa in the complexes of all sizes. In the severe asthma group, an antigen fraction with a molecular mass of 36 kDa within the small-sized molecular complexes was revealed. Conclusion. The observed increase of small and medium-sized circulating immune complexes serum levels in patients with bronchial asthma may be an indicator of of these patients predisposal to autoimmune reactions development.

scholarly journals Indicators of total homeostasis in cows in different periods of lactations

2021 ◽  
Vol 51 (2) ◽  
pp. 56-64
Author(s):  
E. A. Dementyeva ◽  
S. N. Mager
Keyword(s):  
Immune Complexes ◽  
Relative Number ◽  
The Body ◽  
Mononuclear Phagocytes ◽  
Circulating Immune Complexes ◽  
High Rate ◽  
Control Group ◽  
Dry Period ◽  
Birth Process ◽  
Significant Difference

The immunomorphological parameters of cattle in different periods of lactation were studied. In the first 3 months of lactation, excluding 7 days of the colostrum period, 59.3 units of circulating immune complexes were found in the serum, in the middle of lactation (4–7 months) – 94.2 (p <0.05), at the end (8–10 months) – 94.1 units (p <0.05). The significant difference between the indicator in the first 3 months and in the subsequent periods of lactation is due to the fact that at the beginning of lactation the cows were not yet pregnant. In the colostrum period, a high rate of circulating immune complexes of 116.1 units (p <0.05) was determined as a consequence of a fetal prenatal immune attack on the cow's body, when the system of mononuclear phagocytes had not yet coped with the elimination of neutralization products. During the dry period, the number of circulating immune complexes was 87.6 units (p <0.05). The decrease in the indicator occurred due to an increase in the activity of mononuclear phagocytes and the absence of lactation effect on the body. The content of segmental, functionally mature leukocytes at the beginning of lactation was 39.4%, in the middle of lactation this indicator decreased to 24.8% (p<0.05), at the end it was 26.3% (p<0.05). A significant difference was found in the relative number of segmented neutrophils in the control group and in non-lactating cows in the dry period – 29.9% (p <0.05). During the colostrum period, the level of lymphocytes in the blood of animals was 62.0% (p <0.05) and it significantly differed from the control – 43.6%. In the middle and at the end of lactation, there was also a significant difference between the indicator and the control, up to 58.9–59.4% (p<0.05). A significant difference with the group of down-calving dry cows was established – 53.9% (p <0.05). In the first 1–3 months of lactation, cows are either not yet pregnant, or a close bond (placenta) between the mother and the fetus has not yet been formed, therefore a low activity of specific immunity at this time is caused by the absence of foreign fetal antigens in the blood of cows. The findings suggest that the birth process may be initiated by the immune system.

Thrombangitis obliterans: Subpopulationen der Leukozyten und zirkulierende Immunkomplexe

VASA ◽  
2001 ◽  
Vol 30 (3) ◽  
pp. 189-194 ◽  
Author(s):  
Knut Kröger ◽  
E. Kreuzfelder ◽  
C. Moser ◽  
F. Santosa ◽  
C. Buss ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Mononuclear Cells ◽  
Binding Capacity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Reactive Protein ◽  
Hla Dr ◽  
Acral Ischemia ◽  
Zirkulierende Immunkomplexe

Background: The etiology of thrombangitis obliterans is still unclear. Although cellular infiltration of the vessel wall is known, no studies on peripheral blood mononuclear cells are reported. Therefore, we assessed leucocyte subpopulations and circulating immune complexes in patients with thrombangitis obliterans and a control group of normal people. Patients and methods: 31 patients (40 ± 2 years, 24 male, 7 female) with thrombangitis obliterans were included, based on the following criteria: age of manifestation, acral ischemia in legs and arms, previous thrombophlebitis or phlebitis saltans. Manifestation of atherosclerosis or other vasculitic manifestations were excluded. Leucocyte subpopulations, levels of C-reactive protein (CRP) and circulating immune complexes (CIC) were investigated. An age-matched control group (n = 25) was recruited from voluntary blood donors. Results: Leucocyte counts in the thrombangitis group (mean ± SD: 10839 ± 782/nl) were significantly different from the control group (6205 ± 414/nl, p < 0.0001). The same was true for absolute counts of granulocytes, monocytes and lymphocytes. The results were independent from CRP, which was elevated only in 6 patients. Relative counts of naive helper T-cells were significantly lower in the patient group. HLA-DR expression on B-cells was lower on the patients’ lymphocytes. The concentrations of IgA, IgG and IgM in CIC were higher in the thrombangitis patients compared to the control group. C1q-binding capacity and phosphatidylserine antibodies showed no differences. Conclusions: Patients suffering from thrombangitis obliterans show alterations of leucocyte counts and their subpopulations as well as alterations of the humoral (IgCIC) immune system.

scholarly journals Role of Humoral Mechanisms in Etiology of Lichen Planus

PRILOZI ◽  
2014 ◽  
Vol 35 (3) ◽  
pp. 185-194
Author(s):  
Mirjana Popovska ◽  
Kristina Mitik ◽  
Ladislava Grchevska ◽  
Aneta Atanasovska-Stojanovska ◽  
Biljana Kapushevska ◽  
...  
Keyword(s):  
Immune Response ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Humoral Immune Response ◽  
Immunoglobulin A ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Humoral Immune

AbstractAim: To examine the role of IgA, CIC and component C3 as indicators of humoral immune response in the etiopathogenesis of oral erosive lichen planus (OELP).Material and method: The study comprised 19 patients with OELP whose samples of blood, saliva and tissue were obtained after carefully taken medical history and clinical examination. Samples of oral mucosa were taken from the site of lesion, i.e. exclusively from buccal mucosa (1 cm in width and length), and from the deep epithelium as well as a segment from the lamina propria. Determination of immunoglobulins in serum and saliva, and determination of component C3, was done using the micro-elisa technique by Rook & Cameron, Engvall and Ulman. Determination of CIC in serum and mixed saliva was done with the PEG (polyethylene glycol) method. Determination of immunoglobulin A and component C3 in biopsy material was done with direct immunofluorescence.Results: Levels of immunoglobulin A in serum in OELP during exacerbation were decreased (1.04 ± 0.49 gr/l) and during remission increased (5.92 ± 0.62) in comparison with the control group (p < 0.001). Levels of CIC during exacerbation and remission were increased (p < 0.001), and component C3 levels were increased in both examined phases in the examined group compared with the control group (p < 0.05). Deposits of IgA were registered in one (5.88%) patient with OELP and component C3 was registered in 3 (17.64%) patients.Conclusion: Changes in IgA values, as well as CIC and component C3, may correlate with changes in oral mucosa emphasizing the role of humoral immune response in the pathogenesis of oral lichen planus.

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