scholarly journals Investigation of rs531564 Polymorphism in the Primary MicroRNA-124 Gene in Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis: Association with Disease Susceptibility and Clinical Characteristics

2021 ◽  
Author(s):  
Mehdi Hassani ◽  
Mohammad Dehani ◽  
Maryam Zare Rafie ◽  
Emran Esmaeilzadeh ◽  
Saeideh Davar ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Clinical Characteristics ◽  
Control Group ◽  
Systemic Lupus ◽  
Reactive Protein ◽  
Genotype Frequencies ◽  
Dsdna Antibody ◽  
Microrna 124

MicroRNA-124 (miR-124) is known as an important regulator of the immune system and inflammatory response. Studies have reported that this miRNA is dysregulated in autoimmune disorders such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). A functional analysis demonstrated that rs531564 (C>G) affects the biogenesis of primary microRNA transcript-124 (pri-miR-124) and changes the expression of mature miR-124. In the present study, for the first time, we intended to evaluate the possible association between rs531564 polymorphism with SLE and RA risk. In this case-control study, 110 patients with SLE, 115 patients with RA, and 120 healthy subjects were enrolled to evaluate rs531564 genotypes with real-time polymerase chain reaction (PCR) high resolution melting method. Our findings demonstrated that frequency of GC genotype and G allele were considerably higher in the control group than RA patients, demonstrating that that GC genotype and G allele have a protective effect for healthy individuals (GC vs CC; OR: 0.29; 95%CI [0.12,0.67] and G vs C; OR: 0.42; 95%CI [0.23,0.78]). However, no significant correlation was confirmed between allele and genotype frequencies of rs531564 with SLE risk (p>0.05). However, the G allele in rs531564 polymorphism was associated with serum level of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), anti-dsDNA antibody, C3, C4, and creatinine, and frequency of renal involvements in SLE patients (p<0.05). Moreover, in RA patients, the G was correlated with lower concentration ESR and CRP (p<0.001). Our findings propose a considerable association between rs531564 polymorphism in the pri-miR124 gene with susceptibility and clinical characteristics of RA and SLE in the Iranian population.

scholarly journals Onset of systemic lupus erythematosus after conversion of infliximab to adalimumab treatment in rheumatoid arthritis with a pre-existing anti-dsDNA antibody level

Rheumatology ◽  
2006 ◽  
Vol 45 (10) ◽  
pp. 1317-1319 ◽  
Author(s):  
A. W. A. M. van Rijthoven ◽  
J. W. J. Bijlsma ◽  
M. Canninga-van Dijk ◽  
R. H. W. M. Derksen ◽  
J. A. G. van Roon
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Antibody Level ◽  
Lupus Erythematosus ◽  
Systemic Lupus ◽  
Dsdna Antibody ◽  
Adalimumab Treatment

scholarly journals Spondyloarthropathies in Autoimmune Diseases and Vice Versa

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Oscar M. Pérez-Fernández ◽  
Rubén D. Mantilla ◽  
Paola Cruz-Tapias ◽  
Alberto Rodriguez-Rodriguez ◽  
Adriana Rojas-Villarraga ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Autoimmune Diseases ◽  
Lupus Erythematosus ◽  
Clinical Characteristics ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Systemic Lupus ◽  
Two Phase ◽  
Cross Sectional

Polyautoimmunity is one of the major clinical characteristics of autoimmune diseases (ADs). The aim of this study was to investigate the prevalence of ADs in spondyloarthropathies (SpAs) and vice versa. This was a two-phase cross-sectional study. First, we examined the presence of ADs in a cohort of patients with SpAs (N=148). Second, we searched for the presence of SpAs in a well-defined group of patients with ADs (N=1077) including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and Sjögren’s syndrome (SS). Among patients with SpAs, ankylosing spondylitis was observed in the majority of them (55.6%). There were two patients presenting with SS in the SpA group (1.4%) and 5 patients with autoimmune thyroiditis (3.5%). The global prevalence of ADs in SpAs was 4.86%. In the ADs group, there were 5 patients with SpAs (0.46%). Our results suggest a lack of association between SpAs and ADs. Accordingly, SpAs might correspond more to autoinflammatory diseases rather than to ADs.

scholarly journals Combined Detection of Mean Platelet Volume and Immunoglobins as a Strategy for the Diagnosis of Systemic Lupus Erythematosus

2019 ◽  
Vol 2 (4) ◽  
pp. 19
Author(s):  
Changzhi Xu ◽  
Zhizhi Xie ◽  
Yanhua Yi ◽  
Donglin Zhu ◽  
Yun Xi
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Renal Impairment ◽  
Lupus Erythematosus ◽  
Mean Platelet Volume ◽  
Test Method ◽  
Control Group ◽  
Systemic Lupus ◽  
Platelet Volume ◽  
Reactive Protein ◽  
Complex Process

Objective:To explore the possibility of diagnosing and monitoring patients with systemic lupus erythematosus (SLE) using the combination of mean platelet volume (MPV) and routine immunoglobulin test. Method:116 patients with SLE were divided into 3 groups according to their clinical characteristics, including 29 patients with renal impairment, 44 cases of active stage and 43 cases of inactive patients. 40 healthy subjects were randomly selected as controls. Subjects were tested for routine blood test and plasma Immunoglobins, such as IgG, IgA, IgM, C3, C4, CH50, CRP. The results were analyzed and the characteristics of each group of subjects were determined, the correlation between test results and diagnosis were studied. Result: In comparison to the control group, the serum level of MPV, C3 and C4 were decreased (P<0.05), and C reactive protein level was elevated (P<0.001) in the three groups of SLE patients. The IgG level in active and inactive SLE patients was increased (P<0.0001), CH50 level was decreased in patients with inactive SLE (P<0.05), IgA level of active SLE subjects was found to be elevated (P<0.05), IgM in patients with renal impairment was decreased (P<0.05). Other than that, no other significant characteristic were found. Conclusion: The pathogenesis of SLE is a complex process involving multiple factors. The changes of MPV, IgG, IgA, IgM, C3, C4, CH50 and CRP in SLE patients are characteristic parameters. The combination of the above indicators can help to determine the diagnosis and staging of SLE. The timely diagnosis and treatment of SLE patients has important clinical significance in protecting the organ function of SLE patients and improving the prognosis.

scholarly journals Comparative analysis of the systemic inflammatory response in rheumatic diseases

2012 ◽  
Vol 93 (1) ◽  
pp. 12-17
Author(s):  
D V Ivanov ◽  
L A Sokolova ◽  
E Yu Gusev ◽  
L N Kamkina ◽  
N O Plekhanova
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Ankylosing Spondylitis ◽  
Inflammatory Response ◽  
Rheumatic Diseases ◽  
Lupus Erythematosus ◽  
C Reactive Protein ◽  
Systemic Lupus ◽  
Reactive Protein ◽  
Reactivity Coefficient

Aim. To compare the course of chronic systemic inflammation during various rheumatic diseases. Methods. Examined were three groups of patients: with ankylosing spondylitis - 25 people (20 males and 5 females), with rheumatoid arthritis - 26 people (11 males and 15 females) and with systemic lupus erythematosus - 49 people (3 males and 46 females). The control group included 50 practically healthy individuals (26 males and 24 females). Analyzed were the following parameters: the content of interleukin-6, -8, -10, C-reactive protein. The integral index of the reactivity coefficient was calculated. Results. The level of the studied cytokines was significantly higher in systemic lupus erythematosus, than in ankylosing spondylitis and rheumatoid arthritis, while the content of C-reactive protein was significantly higher in ankylosing spondylitis and rheumatoid arthritis. The values of the reactivity coefficient were also significantly higher in systemic lupus erythematosus. Conclusion. The presence of systemic inflammation was determined in most patients with systemic lupus erythematosus, while ankylosing spondylitis and rheumatoid arthritis were characterized only by mild manifestations of systemic inflammatory response.

scholarly journals MODULASI SEL PUNCA MESENKIMAL DALAM MENURUNKAN KADAR HIGH SENSITIVITY C-REACTIVE PROTEIN SEBAGAI TERAPI NEFRITIS LUPUS

Biomedika ◽  
2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Indah Putri Maharani ◽  
Zainal Arifin Adnan ◽  
Arief Nurudhin
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
High Sensitivity ◽  
Control Group ◽  
C Reactive Protein ◽  
Systemic Lupus ◽  
Control Group Design ◽  
Group Design ◽  
Reactive Protein ◽  
Post Test

Systemic Lupus Erythematosus merupakan penyakit inflamasi autoimun kronis dengan gambaran klinis luas dan perjalanan penyakit beragam. Pemberian pristan intraperitoneal dapat menginduksi lupus pada mencit. Secretome sel punca mesenkimal bekerja secara parakrin memberikan efek antinflamasi dan imunomodulasi antara lain mensupresi sel T dan sel B autoreaktif. High Sensitivity C-Reactive Protein (hsCRP) terkait dengan patogenesis SLE dan selaras dengan aktifitas penyakit.Tujuan Penelitian adalah untuk mengetahui pengaruh secretome sel punca mesenkimal terhadap kadar hsCRP pada mencit model lupus dengan induksi pristan. Desain penelitian adalah eksperimental dengan randomisasi, post test only control group design, sampel 21 ekor mencit betina Mus Musculus galur Balb/C, dibagi 3 kelompok yaitu kelompok kontrol (injeksi intraperitoneal NaCl 0,9% 0,5 ml), kelompok perlakuan (injeksi pristan intraperitoneal 0,5 ml) dan kelompok terapi (injeksi intraperitoneal pristan 0,5 ml dan secretome 0,45 ml). Penelitian dilakukan selama 3 minggu, secretome diberikan pada akhir penelitian. Sesudah perlakuan dinilai kadar hsCRP secara ELISA. Analisis statistik menggunakan SPSS 22 for windows dengan uji Kruskal-Wallis dilanjutkan Mann-Whitney U test. P bermakna jika p<0,05. Hasil penelitian menunjukkan bahwa rata-rata kadar hsCRP pada ketiga kelompok yaitu kontrol 440.68(110.08-564.29) ng/ mL; perlakuan (pristan) 2964.26(601.13-3926.10) ng/mL; terapi pristan+secretome) 506.93(207.62-1473.46) ng/mL, dengan kemaknaan p=0.008. Terdapat perbedaan bermakna kadar hsCRP antara kelompok pristan vs pristan+secretome (2457.33 ng/mL; p=0.047). Secretome sel punca mesenkimal mampu menurunkan kadar hsCRP pada mencit model lupus dengan induksi pristan.Kata Kunci: High Sensitivity C-Reactive Protein, Nefritis lupus, Secretome

scholarly journals N-acetylcysteine effectively alleviates systemic lupus erythematosus in mice via regulation of oxidative stress

2021 ◽  
Vol 19 (12) ◽  
pp. 2591-2595
Author(s):  
Feng Lu ◽  
Bingxin Liu ◽  
Hui Zhao
Keyword(s):  
Oxidative Stress ◽  
Systemic Lupus Erythematosus ◽  
Superoxide Dismutase ◽  
Lupus Erythematosus ◽  
Serum Levels ◽  
Control Group ◽  
Systemic Lupus ◽  
Urine Protein ◽  
Treatment Groups ◽  
Dsdna Antibody

Purpose: To study the influence of N-acetylcysteine (NAC) on systemic lupus  erythematosus (SLE) mice, and the mechanism(s) involved. Methods: Fourteen MRL/lpr SLE mice aged 5 weeks (mean weight = 20.35 ± 2.12 g) were divided into two 7-mouse groups: SLE (control) and treatment groups. The control group comprised healthy female SPF-grade C57BL/6 mice (n = 7). The treatment group mice received intraperitoneal injection of NAC at a dose of 250 mg/kg daily for 8 weeks. The serum levels of malondialdehyde (MDA) and nitric oxide (NO), and activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), were assayed using standard methods. The level of urine protein and activity of anti-double stranded (ds) DNA antibody were determined using their respective enzyme-linked assay (ELISA) kits. Results: The spleens of mice in SLE mice were significantly enlarged, relative to control mice, but they were reduced significantly by NAC (p < 0.05). N-Acetylcysteine (NAC) also significantly reduced the serum levels of MDA and NO in SLE mice, but significantly  increased the serum activities of superoxide dismutase and GPx. Moreover, urine protein concentration and activity of anti-dsDNA antibody in SLE mice significantly increased, but reduced significantly by NAC treatment (p < 0.05). Conclusion: These results suggest that NAC effectively alleviates SLE in mice via regulation of oxidative stress. Thus, NAC has the potentials for development into a therapy for the management of SLE. Keywords: Anti-dsDNA antibodies, Antioxidant enzymes, N-acetylcysteine, Oxidative stress, Systemic lupus erythematosus

scholarly journals Pregnancy outcomes in patients with rheumatoid arthritis and systemic lupus erythematosus. Part I. Maternal outcomes

2019 ◽  
Vol 57 (2) ◽  
pp. 180-185 ◽  
Author(s):  
N. M. Kosheleva ◽  
E. V. Matyanova ◽  
E. V. Fedorova ◽  
N. I. Klimenchenko
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Pregnancy Outcomes ◽  
Control Group ◽  
Maternal Outcomes ◽  
Third Trimester ◽  
Systemic Lupus ◽  
Arterial Perfusion

Improved diagnosis and treatment of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) contribute to the remission of the disease and improve the quality of life of patients. In this regard, more and more women with RA and SLE decide to have pregnancy, which makes it actual to study the features of its course and outcomes in these diseases.Objective: to evaluate maternal pregnancy outcomes in patients with RA and SLE.Subjects and methods. 76 cases of pregnancy were traced prospectively in 72 patients: 32 pregnancies in 29 patients with RA and 44 pregnancies in 43 patients with SLE.Results and discussion. 72 of 76 (94.7%) supervised pregnancies ended in childbirth with the birth of a viable baby. There were three cases of pregnancy loss in the second trimester in SLE patients with concomitant antiphospholipid syndrome (AFS) and one case (3.1%) of perinatal infant death (a boy and a girl, monochorionic diamniotic twins with reverse arterial perfusion syndrome) in a patient with seropositive RA. Compared with the all-Russian population, the supervised RA and SLE patients more often had prematurely birth (37.5‰, 18.7% and 22.7%) and caesarean section (CS; in 236.7‰, 50%, and 56.8% respectively). In the SLE group CS was conducted due to the emergency reasons more frequently than in RA, (respectively 47,7% and 25%, relative risk of 1.9 [1; 3.7]; p=0.04). CS at the first birth was performed more often during RA and SLE than before the onset of the disease (p<0.001). Preeclampsia in patients with RA was diagnosed more often than in the population (9.4% and 15.7 per 1000 births, respectively). There was a reverse correlation between the timing of delivery and disease activity according DAS28-CRP in II (r= 0.5; p=0.01) and III (r= 0.6; p=0.0005) trimesters of pregnancy, and in patients with moderate and high activity of RA in the third trimester (n=12) delivery was earlier than in the control group (n=20), remission or low activity of RA (p<0.01). In patients with SLE who had birth prematurely (22.7%), the duration of the disease (p=0.02) and the duration of oral glucocorticoid therapy (p=0.003) were greater compared with SLE patients having term birth (70.5%); the dose of glucocorticoids at the time of conception and delivery did not affect the timing of delivery.Conclusion. Planning of pregnancy in patients with RA and SLE, monitoring during pregnancy and timely correction of therapy contribute to uncomplicated course of gestation and improve maternal outcomes.

ROLE OF INFLAMMATION AND PLATELETS ACTIVATION IN ATHEROSCLERISIS PROGRESSION IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

2018 ◽  
Author(s):  
А.В. Аршинов ◽  
Н.Ю. Левшин ◽  
И.Г. Маслова ◽  
А.Н. Лужинский
Keyword(s):  
Risk Factors ◽  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Intima Media Thickness ◽  
Control Group ◽  
Systemic Lupus ◽  
Reactive Protein ◽  
Traditional Risk Factors ◽  
Atherosclerosis Development ◽  
Aggregation Activity

Цель исследования: выявить взаимосвязь между дислипидемией, активностью воспаления и функцией тромбоцитов в развитии атеросклероза у пациентов с системной красной волчанкой (СКВ), а также оценить сходство и различия механизмов атерогенеза у больных СКВ и ишемической болезнью сердца. Материалы и методы. Обследовано 102 женщины, из них — 50 больных СКВ, 31 — с инфарктом миокарда (ИМ); контрольную группу составили здоровые женщины (n = 21). Определяли показатели липидного спектра, содержание антител к окисленным липопротеинам низкой плотности (АТ-оксЛПНП), высокочувствительного С-реактивного белка (вчСРБ), интерлейкина 6 (ИЛ-6), тромбоцитарного фактора 4 (ТФ4), агрегационную функцию тромбоцитов и толщину комплекса интима-медиа (ТКИМ) общих сонных артерий. Результаты. У больных СКВ и у пациентов с ИМ выявлено значительное увеличение ТКИМ сонной артерии и выраженная активация воспаления: повышение содержания вчСРБ, ИЛ-6 и увеличение СОЭ. За исключением значений ИЛ-6, лабораторные показатели воспаления у больных СКВ и с ИМ достоверно не различались. Также у пациентов с СКВ и ИМ установлена значительная активация тромбоцитов (достоверный рост содержания ТФ4). Несмотря на наличие дислипидемии в обеих группах, у больных СКВ данные изменения были выражены более отчётливо и сопровождались повышением уровня АТ-оксЛПНП. Заключение. Кроме традиционных факторов риска развития сердечно-сосудистых заболеваний, ассоциация между СКВ и атеросклерозом может быть объяснена дополнительными факторами риска — воспалением и аутоиммунными процессами. Aim: to reveal a relationship between dyslipidemia, infl ammatory activity and platelets reactivity in atherosclerosis development in patients with systemic lupus erythematosus (SLE) and also to assess the similarity and diff erences of atherogenesis mechanisms in patients with SLE and ischemic heart disease. Materials and methods. The study included 102 women: 50 patients with SLE, 31 — with myocardial infarction (MI); control group included 21 healthy women. We measured parameters of lipid spectrum, levels of antibodies against oxidized low density lipoproteins (oxLDL), high-sensitive C-reactive protein (hsCRP), interleukin 6 (IL-6), platelets factor 4 (PF4), platelets aggregation activity and complex intima-media thickness (TCIM) of carotid arteries. Results. Patient with SLE and MI had markedly increased TCIM. Increased infl ammation activity was the second sign of two groups of patients, including increased hsCRP, IL-6, erythrocyte sedimentation test. Laboratory signs of infl ammation did not markedly diff er in two groups except IL-6. Our study also revealed considerable platelets activation in patients with SLE and MI (signifi cant growth of PF4 content). Despite dyslipidemia, all indicated changes were more clearly expressed in patients with SLE; they were accompanied by increased level of antibodies against oxLDL. Conclusion. Except traditional risk factors for cardiovascular diseases development the association between SLE and atherosclerosis. can be explained by additional risk factors — infl ammation and autoimmune processes.

Lack of Association of C-C Chemokine Receptor 5 Δ32 Deletion Status with Rheumatoid Arthritis, Systemic Lupus Erythematosus, Lupus Nephritis, and Disease Severity

2010 ◽  
Vol 37 (11) ◽  
pp. 2226-2231 ◽  
Author(s):  
HENK A. MARTENS ◽  
SACHA GROSS ◽  
GERRIT van der STEEGE ◽  
ELISABETH BROUWER ◽  
JO H.M. BERDEN ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Lupus Nephritis ◽  
Disease Severity ◽  
Chemokine Receptor ◽  
Lupus Erythematosus ◽  
Disease Susceptibility ◽  
Systemic Lupus ◽  
Genotype Frequencies ◽  
Chemokine Receptor 5

Objective.C-C chemokine receptor 5 (CCR5) plays an important role in inflammation. A 32 base-pair (Δ32) deletion in the CCR5 gene leads to a nonfunctional receptor. This deletion has been reported to have a protective effect on the development and progression of several autoimmune diseases. We investigated whether the Δ32 deletion is associated with disease susceptibility in a population of patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and lupus nephritis (LN); and whether it is associated with disease severity.MethodsDNA samples from 405 RA patients, 97 SLE patients, 113 LN patients, and 431 healthy controls were genotyped for the CCR5 Δ32 deletion. Differences in genotype frequencies were tested between patients and controls. Association of genotypes with disease severity was analyzed.ResultsGenotype frequencies of each group were in Hardy-Weinberg equilibrium. The genotype frequencies of patients did not differ significantly from controls (CCR5/Δ32, Δ32/Δ32: RA 18.3% and 1.2%, respectively; SLE 17.5% and 2.1%; LN 13.3% and 1.8%; controls 20.0% and 2.8%). However, there was a trend for lower Δ32 deletion allele frequency in LN patients compared to controls (p = 0.08). There was no significant association between the CCR5 status and disease severity in RA, SLE, or LN.Conclusion.Although an association with LN cannot be excluded, the CCR5 Δ32 deletion does not seem to be a disease susceptibility genotype for RA, SLE, or LN. No significant effect of the Δ32 deletion on disease severity was demonstrated.

Autoantibodies to Tumor Necrosis Factor in Patients with Rheumatoid Arthritis and Systemic Lupus Erythematosus

2009 ◽  
Vol 36 (4) ◽  
pp. 753-756 ◽  
Author(s):  
BARBARA J. ROSENAU ◽  
PETER H. SCHUR
Keyword(s):  
Rheumatoid Arthritis ◽  
Systemic Lupus Erythematosus ◽  
Tumor Necrosis Factor ◽  
Lupus Erythematosus ◽  
Tumor Necrosis ◽  
Clinical Laboratory ◽  
Systemic Lupus ◽  
Reactive Protein ◽  
Markers Of Inflammation ◽  
Necrosis Factor

Objective.To detect autoantibodies to tumor necrosis factor (TNF) in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), and to determine their clinical correlates.Methods.Ninety-two patients with RA and 62 with SLE were studied. Sera were examined for autoantibodies to TNF by enzyme linked immunoassay. Levels of these autoantibodies were analyzed in respect to markers of inflammation such as erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and joint erosions, as well as other clinical, laboratory, and therapeutic aspects of RA and SLE.Results.Anti-TNF levels were higher in those RA patients without erosions, but did not correlate with ESR or CRP.Conclusion.These observations suggest that autoantibody anti-TNF may be part of the innate immune system and may contribute to decreased inflammation in patients with RA.

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