scholarly journals THE STRUCTURE OF THE VARIABILITY OF SOME AROMATIC PLANTS ON THE CONTENT OF TOTAL ANTIOXIDANTS IN THE ECOLOGICALGEOGRAPHICAL EXPERIMENT

2019 ◽  
pp. 87-90
Author(s):  
F. I. Islamova ◽  
A. M. Musaev ◽  
G. K. Radzhabov

The work presents the results of the study of the antioxidant activity of fruits (clapper seeds) of aromatic plants, (Apiaceae, Lindl.): dill (Anethum graveolens L.) and coriander (Coriandrum sativum L.). The plant materials for determining the total content of antioxidants were collected during an ecological-geographical experiment at the Gunib and Tsudakhar experimental bases of the Mountain Botanical Garden of the Dagestan Scientific Center, Russian Academy of Sciences, at altitudes of 1100 and 1730 meters above sea level, simulating the conditions of mountain-valley and upper mountain climate zones, respectively. Determination of the total content of antioxidants in aqueous solutions was performed by the amperometric method on “Tsvet Yauza 01 -AA” instrument. Studies have shown the variability of accumulation of antioxidants, depending on the type, variety, height above sea level of the place of growth of the sample. In terms of the total content of antioxidants, the effect of the cultivation area substantially exceeded the inter-variety differences. In the studied species, intervarietal differences appeared more clearly in A. graveolens, which correlated with the latitude of the range and the ecological amplitude of growth of these species in natural habitats. The obtained data on the study of antioxidant activity in some aromatic medicinal plants allow to evaluate their biological value, to recommend more distinguished samples for cultivation. Plant materials with a high accumulation of antioxidants that can neutralize the harmful effects of oxidative stress can be used as a source of natural polyphenols, natural antioxidants in food. This methodical approach, demonstrated in the work, can serve as a methodological basis for studying the “genotype x environment” interaction and express assessment of varieties, clones, populations, and other units of accounting for the stability and sustainability of the parameters studied, including the content of antioxidants.

2019 ◽  
pp. 127-137 ◽  
Author(s):  
Любовь (Lyubov') Николаевна (Nikolaevna) Скрыпник (Skrypnik) ◽  
Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova)

The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic acid) radical and FRAP (ferric reducing antioxidant power) assays. It was established that the fruits of plants of the genus Sambucus L. were characterized by the maximum level of all studied antioxidants. High content of anthocyanins and phenolic compounds was determined in the leaves of elderberry and dwarf elder. The flowers of these elderberry species were distinguished by a high total content of water-soluble antioxidants. The highest antioxidant activity was observed in fruits extracts in comparison with other parts of the plant. Higher antioxidant activity was identified in the extract of the fruits of elderberry and dwarf elder than of the red elderberry fruits. The most optimal method for evaluating the antioxidant activity of elder extracts was the FRAP assay, which showed the highest correlation between AOA and individual antioxidant components, compared to DPPH and ABTS assays. Comparative analysis of antioxidant content and antioxidant activity of various plant parts of three elderberry species showed that the most promising sources of biologically active substances with antioxidant properties are fruits and flowers of elderberry and dwarf elder.


10.5219/1715 ◽  
2022 ◽  
Vol 16 ◽  
pp. 1-14
Author(s):  
Olena Vergun ◽  
Liudmyla Svidenko ◽  
Olga Grygorieva ◽  
Vladimíra Horčinová Sedláčková ◽  
Katarína Fatrcová Šramková ◽  
...  

  This scientific work was aimed to evaluate the antioxidant potential of aromatic plants of Thymus spp. in the East of Ukraine. These plants are known as medicinal and food around the world. All antioxidant parameters were investigated spectrophotometrically: total content of polyphenols (TPC), the total content of phenolic acids (TPAC), the total content of flavonoids (TFC), molybdenum reducing power of extracts (MRP), and antioxidant activity by DPPH method (DPPH). Investigation of ethanolic extracts demonstrated that TPC varied from 57.89 to 123.67 mg/g gallic acid equivalent (GAE) DW for Th. pulegioides, from 61.43 to 168.18 mg GAE/g for Th. serpyllum, and from 47.36 to 115.67 mg GAE/g for Th. vulgaris. TPAC ranged from 27.36 to 50.22 mg/g caffeic acid equivalent (CAE) DW for Th. pulegioides, from 28.58 to 59.62 mg CAE/g for Th. serpyllum, and from 22.95 to 53.82 mg CAE/g for Th. vulgaris. TFC was determined in a range from 29.88 to 61.23 mg/g quercetin equivalent (QE) DW for Th. pulegioides, from 36.0 to 82.43 mg QE/g for Th. serpyllum, and from 24.59 to 55.41 mg QE/g for Th. vulgaris. MRP was detected in the range of 94.65 – 204.76 mg/g Trolox equivalent (TE) DW for Th. pulegioides, 96.06 – 219.0 mg TE/g for Th. serpyllum, and 87.56 – 215.43 mg TE/g for Th. vulgaris. The antioxidant activity of extracts by the DPPH method was 6.34 – 9.23 mg TE/g for Th. pulegioides, 8.11 – 9.21 mg TE/g for Th. serpyllum, and 4.97 – 9.53 mg TE/g for Th. vulgaris. It was established that polyphenol accumulation depended on the growth stage and species. For all species was found a strong correlation between TPC and TFC (r = 0.938, 0.908, and 0.854). Investigated Thymus spp. are a valuable source of antioxidants that can be used in pharmacological studies and the food industry.


Author(s):  
N. V. Makarova ◽  
N. B. Eremeeva

The aim of the work is to develop an optimal technology for extracting a complex of substances with an antioxidant effect from cranberries (Vaccinium oxycoccos L.), sea buckthorn (Hippophaërhamnoides L.), blackberries (Rubus subgen. Rubus), honeysuckle (Lonicera L.) widespread in the Russian Federation. ), viburnum (Viburnum opulus L.), mountain ash (Sorbus aucuparia L.), juniper (Juniperus L.), a comparative study of the effect of ultrasound exposure with traditional maceration and microwave irradiation on the total content of phenols, flavonoids, anthocyanins, β-carotene, antiradical e action, restoring force in the extraction of the studied plant materials. Spectrophotometric methods for determining the total content of phenols, flavonoids, anthocyanins, antiradical activity with 2,2-diphenyl-1-picrylhydrazyl free radical, and restoring forces with the FRAP reagent were chosen as research methods. It is the use of ultrasonic extraction for fruits that allows to obtain a higher content of phenols, flavonoids, anthocyanins, antiradical activity values, regenerating power, antioxidant action in the obtained extracts. Microwave radiation has a similar effect in the level of a number of indicators of fruit extracts. Although the performance of microwave extracts of fruits is lower in magnitude than ultrasonic extracts. It is interesting to note that it is anthocyanins as the most sensitive to external influences class of compounds that are extracted during ultrasonic processing from the berries and remain the most. To obtain fruit extract, acting as a component of many dietary supplements, as well as cosmetics with a high level of antioxidant substances and antioxidant activity, on the basis of the conducted research it is possible to recommend ultrasonic treatment as an intensification method with the same temperature parameters and process time, which will allow to obtain extracts with a higher content of nutraceutical substances.


The article presents the results of studying the qualitative composition and quantitative content of some groups of phenolic compounds in 11 types of medicinal plants growing in the foothills of the North Caucasus, and provides the estimates of the antioxidant activity of extracts from these plants. The qualitative and quantitative content of phenolic compounds was determined using a Kapel-105M capillary electrophoresis system, and the total antioxidant activity of the extracts was measured on a Tsvet Yauza-01-AA device with an amperometric detector. In the studied plant samples, the total content of tannins was determined, eight phenolcarbonic acids were identified and quantified, as well as quercetin and rutin — two of the most important flavonols. The highest total content of phenolcarbonic acids (11,776.2 mg/kg), as well as the highest antioxidant activity were noted in the aqueous extract obtained from Echinacea purpurea (lat. Echinacea angustifolia). The direct relationship between the antioxidant activity of the studied medicinal raw material and the content of phenolic compounds has been experimentally established as follows: the higher is the concentration of phenolic substances, the higher is the antioxidant activity. The results of this study provide new information on the composition and content of phenolic compounds in some types of wild-growing plant raw materials of the North Caucasus and the antioxidant activity of extracts based thereon that will facilitate the use of the studied plants as a potential source of natural antioxidants in the production of functional materials.


2021 ◽  
Vol 17 (5) ◽  
pp. 43-52
Author(s):  
A. A. Skhalyakhov ◽  
Kh. R. Siyukhov ◽  
Z. T. Tazova ◽  
L. V. Lunina

In the last decade the attitude of socially active people to their own health has changed significantly. The desire to lead a healthy lifestyle forms consumers’ demand for proper balanced nutrition, products enriched with natural biologically active substances of unconventional plant materials, systematic consumption of which can not only reduce the risk of alimentary diseases, but also protect a person from oxidative stress, which is the predecessor of many serious illnesses. Among the wide range of chemical compounds that make up plant materials, a special place is occupied by compounds of antioxidant action, such as flavonoids, phenol carboxylic acids, vitamins C and E. The aim of the research was to study the qualitative composition and quantitative content of natural antioxidants in some types of medicinal and technical plant materials of the Republic of Adygea to determine the possibility of their use in the production of functional food products. The content of biologically active compounds that determine the antioxidant properties of plant materials was determined using «Kapel 105 «M» capillary electrophoresis system and JASCO 875-UV liquid chromatograph. Antioxidant activity was measured by the amperometric method with Tsvet Yauza-01-AA analyzer. According to the results of the research, it has been found that Echinacea purpurea herb (1,09 g / dm3) and walnut leaves (0,96 g / dm3) exhibit a high antioxidant activity (in terms of gallic acid), which is due to the high concentration of natural antioxidants of the phenolic type. Due to these properties this plant raw materials can be used as enriching ingredients for the production of various groups of food products for functional purposes.


2019 ◽  
Vol 86 ◽  
pp. 103914
Author(s):  
Éva Németh-Zámboriné ◽  
Péter Rajhárt ◽  
Katarzyna Seidler-Łożykowska ◽  
Zsuzsanna Pluhár ◽  
Krisztina Szabó

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.


Facies ◽  
2021 ◽  
Vol 67 (3) ◽  
Author(s):  
Markus Wilmsen ◽  
Udita Bansal

AbstractCenomanian strata of the Elbtal Group (Saxony, eastern Germany) reflect a major global sea-level rise and contain, in certain intervals, a green authigenic clay mineral in abundance. Based on the integrated study of five new core sections, the environmental background and spatio-temporal patterns of these glauconitic strata are reconstructed and some general preconditions allegedly needed for glaucony formation are critically questioned. XRD analyses of green grains extracted from selected samples confirm their glauconitic mineralogy. Based on field observations as well as on the careful evaluation of litho- and microfacies, 12 glauconitc facies types (GFTs), broadly reflecting a proximal–distal gradient, have been identified, containing granular and matrix glaucony of exclusively intrasequential origin. When observed in stratigraphic succession, GFT-1 to GFT-12 commonly occur superimposed in transgressive cycles starting with the glauconitic basal conglomerates, followed up-section by glauconitic sandstones, sandy glauconitites, fine-grained, bioturbated, argillaceous and/or marly glauconitic sandstones; glauconitic argillaceous marls, glauconitic marlstones, and glauconitic calcareous nodules continue the retrogradational fining-upward trend. The vertical facies succession with upwards decreasing glaucony content demonstrates that the center of production and deposition of glaucony in the Cenomanian of Saxony was the nearshore zone. This time-transgressive glaucony depocenter tracks the regional onlap patterns of the Elbtal Group, shifting southeastwards during the Cenomanian 2nd-order sea-level rise. The substantial development of glaucony in the thick (60 m) uppermost Cenomanian Pennrich Formation, reflecting a tidal, shallow-marine, nearshore siliciclastic depositional system and temporally corresponding to only ~ 400 kyr, shows that glaucony formation occurred under wet, warm-temperate conditions, high accumulation rates and on rather short-term time scales. Our new integrated data thus indicate that environmental factors such as great water depth, cool temperatures, long time scales, and sediment starvation had no impact on early Late Cretaceous glaucony formation in Saxony, suggesting that the determining factors of ancient glaucony may be fundamentally different from recent conditions and revealing certain limitations of the uniformitarian approach.


2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.


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