Serum Biochemical (Total Protein, Cholesterol) Status Before and After Treatment in Crossbred Cattle

Background: The aim of this study was to estimate the serum biochemical (glucose, cholesterol, total protein, calcium, phosphorus and magnesium) and hormonal constituents (estrogen and progesterone) in female Manipuri pony mares during the different days of oestrous cycle. Methods: A total of 50 numbers of blood samples were collected from 10 numbers of apparently healthy, non-pregnant on the different days of oestrous cycle i.e. day 0, 4, 8, 14 and 18 by using commercial diagnostic kits. Result: The mean serum levels of glucose, cholesterol and phosphorus were found to be non-significant on the different days of oestrous cycle whereas the mean serum level of total protein and calcium was found significantly different (P less than 0.01). The serum magnesium level differed significantly (P less than 0.05) amongst the different days of oestrous cycle. Both estrogen and progesterone were also found to be significant (P less than 0.01) between the different days of oestrous cycle. The results showed significant influence on serum biochemical and hormonal assay during different days of oestrous cycle in female Manipuri pony.

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Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old

Biotechnology in Animal Husbandry ◽

10.2298/bah2003329g ◽

2020 ◽

Vol 36 (3) ◽

pp. 329-339

Author(s):

Nguyen Giang ◽

Nguyen Hien ◽

Huynh Loan ◽

Phan Phuc ◽

Nguyen Dai ◽

...

Keyword(s):

Total Protein ◽

Biochemical Parameters ◽

Animal Health ◽

Correlation Coefficients ◽

Biochemical Properties ◽

Blood Samples ◽

Male And Female ◽

Native Breed ◽

Serum Biochemical ◽

The Relationship

Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.

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Age – Related changes in serum biochemical parameters in the domestic rabbit (Oryctolagus cuniculus)

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i5.211 ◽

2020 ◽

Vol 45 (5) ◽

pp. 67-71

Author(s):

K. A. Adebisi

Keyword(s):

Total Protein ◽

Biochemical Parameters ◽

Serum Biochemistry ◽

Serum Biochemical Parameters ◽

Serum Total Protein ◽

Domestic Rabbit ◽

Age Related ◽

Protein 4.2 ◽

Pubertal Stages ◽

Serum Biochemical

The influence of age on serum biochemical components in the domestic rabbit was investigated. The serum total protein (g/dL), albumin (g/dL), globulin (g/dL), creatinine (mg/dL), urea mg/dL, cholesterol (mg/dL), Alkaline phosphatase (ALP; iu/l), Aspartate aminotranferase (AST; iu/l) and Alanine amimotransferase (ALT; iu/l) were assessed in 24 male rabbits at 7, 14 and 21 weeks of age to represent weaners, growers and pubertal stages. Data were analysed using descriptive statistics and analysis of variance at á . Results of 0.05 serum biochemistry revealed a steady significant increase in total protein (4.2±0.3; 5.3±0.5; 6.9±0.2), albumin (2.2±0.7; 2.8±0.4; 4.0±0.2), globulin (1.9±0.3; 2.5±0.4, 2.9±0.2), cholesterol (49.5±1.1; 56.4±4.2; 62.4±5.4) and creatinine (0.8±0.1; 1.2±0.1; 1.9±0.3) at 7, 14 and 21 weeks respectively. Serum urea levels were similar across the ages. The values recorded for ALP and AST at 7 weeks (39.6±7.5; 101.1±6.7) and 14 weeks (38.7±6.4; 104.5±8.7) were significantly higher than the 21 weeks (33.5±2.7; 95.8±5.9) while ALT at 7 weeks (27.33±5.6) was significantly lower than 14 (38.2±3.9) and 21 weeks (37.4±3.0). The age of the rabbits had a significant effect on their serum biochemical parameters. They should therefore be grouped according to age when setting serum reference values for them.

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Effect of Pantopaque myelography on cerebrospinal fluid fractions

Journal of Neurosurgery ◽

10.3171/jns.1973.38.2.0167 ◽

1973 ◽

Vol 38 (2) ◽

pp. 167-171 ◽

Cited By ~ 8

Author(s):

Darwin J. Ferry ◽

Ronald Gooding ◽

Jim C. Standefer ◽

G. Michael Wiese

Keyword(s):

Cerebrospinal Fluid ◽

Inflammatory Reaction ◽

Total Protein ◽

Colloidal Gold ◽

Gamma Globulin ◽

Content Type ◽

Electrophoretic Patterns ◽

Before And After ◽

Chronic Inflammatory Reaction ◽

Fine Print

✓ Cerebrospinal fluid (CSF) changes induced by Pantopaque myelography were determined by comparing samples of CSF from 47 patients with disc symptoms, obtained before and after myelography. Cell count, total protein, colloidal gold curve, and CSF protein electrophoretic patterns were compared. An immediate and persistent modest lymphocytosis was found. Total protein and CSF gamma globulin were elevated 3 weeks after myelography and throughout the remainder of the 80-day study period. This CSF profile characterizes a chronic inflammatory reaction induced by Pantopaque.

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Failure of myocardial ischemia to increase pulmonary microvascular permeability in dogs

Journal of Applied Physiology ◽

10.1152/jappl.1984.56.3.691 ◽

1984 ◽

Vol 56 (3) ◽

pp. 691-699 ◽

Cited By ~ 1

Author(s):

J. C. Parker ◽

L. Campbell ◽

S. Gilchrist ◽

G. Longenecker ◽

A. E. Taylor

Keyword(s):

Myocardial Ischemia ◽

Extravascular Lung Water ◽

Total Protein ◽

Microvascular Permeability ◽

Lung Water ◽

Permeability Surface ◽

Before And After ◽

Wet Weight ◽

Best Fit ◽

Lung Lymph

Increased extravascular lung water has been reported following periods of myocardial ischemia. To determine whether increased pulmonary microvascular permeability was produced by ischemia, total protein lymph-to-plasma concentration ratios (CL/CP) were obtained at mechanically increased left atrial pressures (Pla) before and after ligation of the left anterior descending coronary artery in dogs. Pulmonary and systemic vascular pressures and cardiac output were monitored and lymph flow was measured from an afferent tracheobronchial lymphatic. Osmotic reflection coefficients (sigma) for total protein were estimated using CL/CP = 1-sigma at high filtration rates, and permeability-surface area (PSf) products were fit to the data. The postischemic lung lymph data best fit average values of sigma = 0.68 and PSf = 0.073 ml X min-1 X 100 g-1 wet weight. There were no significant differences in lymph protein or water clearances between the pre- and postischemic increased Pla states or for myocardial ischemia compared with control values for the experimental preparation. Levels of 6-ketoprostaglandin F1 alpha, a degradation product of prostacyclin, increased by 10- to 14-fold above preischemic values in pulmonary lymph, and there was a significant increase in pulmonary vascular resistance during ischemia. Extravascular lung water was not increased above that attributed to the increased Pla alone. These data indicate no significant increase in pulmonary microvascular permeability to plasma proteins during myocardial ischemia.

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Influence of posture on free calcium and related variables.

Clinical Chemistry ◽

10.1093/clinchem/25.10.1766 ◽

1979 ◽

Vol 25 (10) ◽

pp. 1766-1769 ◽

Cited By ~ 18

Author(s):

B W Renoe ◽

J M McDonald ◽

J H Ladenson

Keyword(s):

Total Protein ◽

Hydrogen Ion ◽

Free Calcium ◽

Total Calcium ◽

Healthy Men ◽

Supine Posture ◽

Before And After ◽

The Subject ◽

Sodium And Potassium ◽

Total Magnesium

Abstract We measured free calcium and related variables before and after the subject changed from the upright to the supine posture, doing 15 separate such experiments on 11 healthy men. After such a change, free calcium (1.7 +/- 0.4%), total calcium (4.6 +/- 0.7%), total protein (11.5 +/- 1.4%), albumin (12.2 +/- 2.0%), total magnesium (3.8 +/- 0.9%), and the activity of hydrogen ion (2.9 +/- 1.0%) decreased significantly (values are means +/- SEM), but promptly reverted when three subjects assumed the alternative posture. Changes in lactate values were not rapidly reversible; sodium and potassium showed no significant change. The mechanism of the changes in free calcium is unclear, but they correlated only with the changes in total calcium and were notably less than the changes in total calcium, indicating that posture will have less effect on the interpretation of free calcium values than on values for total calcium.

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Peritoneal fluid changes in horses subjected to small colon distension

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2011000500001 ◽

2011 ◽

Vol 31 (5) ◽

pp. 367-373 ◽

Cited By ~ 1

Author(s):

Rafael R Faleiros ◽

Delphim G Macoris ◽

Carlos Henrique C Saquetti ◽

Andrea C Aita ◽

Anderson Farias ◽

...

Keyword(s):

Total Protein ◽

Peritoneal Fluid ◽

Clinical Signs ◽

Experimental Period ◽

Blood Chemistry ◽

Control Group ◽

Colon Obstruction ◽

Before And After ◽

Colon Distension ◽

Biochemical Analyses

Intestinal devitalization in cases of small colon obstruction may be difficult to detect based only in clinical signs. The purpose was to serially evaluate blood and peritoneal fluid of horses subjected to small colon distension. Seventeen adult horses were allotted in three groups. In the small colon-distended group (DG, n=7) a surgically-implanted latex balloon was inflated to promote intraluminal small colon distension. In the shamoperated group (SG, n=5), the balloon was implanted but not inflated, and no surgery was done in the control group (CG, n=5). Blood and peritoneal fluid were sampled before and after (6 samples with a 30-minute interval) intestinal obstruction for cytological and biochemical analyses. No significant changes in clinical signs occurred within groups or across time during the experimental period. There were no statistical differences among SG and SG groups in hematologic and blood chemistry variables. Although total protein concentration and lactate dehydrogenase (LDH) activity in peritoneal fluid remained most of the time within reference values during the experimental period in all groups, increases from baseline values were detected in SG and DG groups. Such increases occurred earlier, progressively and with greater magnitude in the DG when compared with the SG (P<0.05). Increases from baselines values were also observed in total nucleated cells and neutrophils counts in the DG (P<0.05). In conclusion, distension of the equine small colon induced progressive subtle increases in total protein and LDH concentrations in the peritoneal fluid during the first hours. Serial evaluation of these variables in peritoneal fluid may be useful for early detection of intestinal devitalization in clinical cases of equine small colon obstruction.

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Nasal Responses to Metabisulfite (MBS) Solution in Non-Atopic Subjects and Patients with Atopic Rhinitis

American Journal of Rhinology ◽

10.2500/105065894782537316 ◽

1994 ◽

Vol 8 (5) ◽

pp. 231-236 ◽

Cited By ~ 3

Author(s):

Mariola Śliwińska-Kowalska ◽

Marek L. Kowalski ◽

Wiestaw Sutkowski ◽

Wiktor Wesotowski ◽

Melvyn R. Danzig

Keyword(s):

Allergic Rhinitis ◽

Total Protein ◽

Clinical Symptoms ◽

Nasal Congestion ◽

Absolute Amount ◽

Protein Ratio ◽

Nasal Symptoms ◽

Before And After ◽

Nasal Secretions ◽

Dose Dependent Increase

In order to characterize the effect of metabisulfite (MBS) on human nasal mucosa, in 10 healthy, non-atopic subjects and in six patients with atopic rhinitis, 50 mg/mL and 100 mg/mL solutions of MBS were sprayed into both nostrils. Clinical symptoms and Nasal Peak Flow (NPF) were recorded. Nasal lavages were performed before and after MBS challenges. MBS challenge induced similar burning/itching sensation in both groups, but rhinorrhea was significantly more intense (P < 0.02) in atopic patients. There was no nasal congestion and no change in NPF after the challenge. Only in atopic patients were nasal symptoms accompanied by a dose-dependent increase in the concentration of total protein and glandular protein lysozyme in nasal washes (P < 0.05). No statistically significant changes were seen in the absolute amount of plasma protein albumin and albumin to total protein ratio (Albumin %) in nasal secretions. This study demonstrates that MBS induces nasal symptoms both in healthy, non-atopic subjects and in patients with allergic rhinitis; but secretory glandular responses are detected only in patients with allergic rhinitis.

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The Effect of Electro-Convulsive Therapy on Spinal Fluid Constituents

Journal of Mental Science ◽

10.1192/bjp.106.443.692 ◽

1960 ◽

Vol 106 (443) ◽

pp. 692-698 ◽

Cited By ~ 10

Author(s):

Samuel Eiduson ◽

Norman Q. Brill ◽

Evelyn Crumpton

Keyword(s):

Total Protein ◽

Brain Function ◽

Cerebral Spinal Fluid ◽

Psychiatric Patients ◽

Spinal Fluid ◽

Convulsive Therapy ◽

Before And After ◽

Electro Convulsive Therapy

During the course of an investigation of the effectiveness of various components of electric convulsive therapy in the treatment of hospitalized psychiatric patients (Brill et al., 1957, 1959) observations were made on the spinal fluid concentrations of cations and total protein before and after treatment. The possibility existed that alterations in brain function and structure (which are believed by many to occur during a course of electro-convulsive treatment, and to be responsible for improvement in patients receiving such treatment) might be associated with, or reflected by measurable changes in the cerebral spinal fluid.

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Influence of salicylate administration on weight gain and serum biochemical parameters in broiler chickens

Medycyna Weterynaryjna ◽

10.21521/mw.432 ◽

2010 ◽

Vol 66 (10) ◽

pp. 675-679 ◽

Cited By ~ 2

Author(s):

Poźniak B. ◽

Yuan H. ◽

J. Yi ◽

Guo C. ◽

Deng S. ◽

...

Keyword(s):

Body Weight ◽

Total Protein ◽

Biochemical Parameters ◽

Broiler Chickens ◽

Clinical Manifestations ◽

Weight Ratio ◽

Serum Biochemical Parameters ◽

Serum Total Protein ◽

Weight Gains ◽

Serum Biochemical

The aim of the study was to investigate the effects of different doses of acetylsalicylic acid (ASA) and sodium salicylate (SS) administration on weight gains and serum biochemical parameters in broiler chickens. This study was performed to investigate the safety of ASA and SS in chickens in conditions of moderate overdose. Seventy five broiler chickens (Sanhuangji breed) were divided into control and experimental groups. The chickens were administered orally with ASA or SS in the following daily doses: 50, 100 and 200 mg/kg for 20 days. In the course of the experiment weight gains were recorded. On day 14 and day 20 the chickens were sampled for blood biochemical analysis. Investigated parameters included serum total protein, albumin, uric acid, creatinine, potassium, sodium and calcium contents as well as alanine transaminase activity. After the last samplings the chickens were dissected and liver and kidney to body weight ratios were recorded. It was demonstrated that SS at a dose of 200 mg/kg increased serum total protein level. ASA increased serum proteins at a dose of 100 mg/kg but not at a higher dose. At the dose of 200 mg/kg ASA increased the kidney to body weight ratio. In all salicylate treated groups the liver weights were lower as compared to the control. No clinical manifestations of intolerance were observed. It is assumed that chickens tolerate ASA and SS well, even in doses producing distinct side effects in several mammalian species.

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