scholarly journals Phenotypic and Molecular Characterization of the Capsular Serotypes of Pasteurella multocida Isolated from Pneumonic Cases of Cattle in Ethiopia

2021 ◽  
Author(s):  
Y.A. Mirtneh ◽  
B.M. Vemulapati ◽  
A. Takele ◽  
Y. Martha ◽  
D. Teferi ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Sampling Method ◽  
Opportunistic Pathogen ◽  
Cross Sectional Study ◽  
Effective Control ◽  
Limited Information ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Adult Cattle

Background: Pasteurella multocida is a heterogeneous species and opportunistic pathogen associated with pneumonia in cattle. Losses due to pneumonia and associated expenses are estimated to be higher in Ethiopia with limited information about the distribution of capsular serotypes. Hence, this study was designed to determine the biotypes and capsular serotypes of P. multocida from pneumonic cases of cattle.Methods: A cross-sectional study with a purposive sampling method was employed in 400 cattle from April 2018 to January 2019. Nasopharyngeal swabs and lung tissue samples were collected from clinically suspected pneumonic cases of calves (n = 170) and adult cattle (n = 230). Samples were analyzed using the bacteriological and molecular assay.Result: Bacteriological analysis revealed isolation of 61 (15.25%) P. multocida subspecies multocida. Molecular assay targeting KMT1 gene (~460 bp) confirmed P. multocida species. Capsular typing revealed the presence of serogroup A (hyaD-hyaC) gene (~1044 bp) and serogroup D (dcbF) gene (~657 bp) in 56 (91.80%) and 5 (8.20%) isolates, respectively. Isolation of P. multocida A:3 highlights its prevalence in the study areas. Therefore, the current finding suggests further comprehensive studies on P. multocida capsular types and microbial diversity of respiratory infection in cattle to design an effective control strategy.

scholarly journals Phenotypic and Molecular Characterization of the Capsular Serotypes of Pasteurella multocida Isolates from Pneumonic Cases of Cattle in Ethiopia

2021 ◽  
Author(s):  
Mirtneh Akalu Yilma ◽  
Murthy Bhadra Vemulapati ◽  
Takele Abayneh Tefera ◽  
Martha Yami ◽  
Teferi Degefa Negi ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Opportunistic Pathogen ◽  
Cross Sectional Study ◽  
Effective Control ◽  
Limited Information ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Adult Cattle ◽  
New Evidence

Abstract Background: Pasteurella multocida is a heterogeneous species and opportunistic pathogen associated with pneumonia in cattle. Losses due to pneumonia and associated expenses are estimated to be higher in Ethiopia with limited information about the distribution of capsular serotypes. Hence, this study was designed to determine the phenotypic and capsular serotypes of P. multocida from pneumonic cases of cattle. Methods: A cross sectional study with purposive sampling method was employed in 400 cattle from April 2018 to January 2019. Nasopharyngeal swabs and lung tissue samples were collected from clinically suspected pneumonic cases of calves (n = 170) and adult cattle (n = 230). Samples were analyzed using bacteriological and molecular assay.Results: Bacteriological analysis revealed isolation of 61 (15.25%) P. multocida subspecies multocida. Incidence was higher in calves 35 (57.38%) compared to adult cattle 26 (42.62%) at P < 0.5. PCR assay targeting KMT1 gene (~460 bp) confirmed P. multocida species. Capsular typing revealed the presence of serogroup A (hyaD-hyaC) gene (~1044 bp) and serogroup D (dcbF) gene (~657 bp) from 56 (91.80%) and 5 (8.20%) isolates, respectively. Isolation of P. multocida serotype A:3 highlights a new evidence in the study areas. Therefore, the current finding suggests further comprehensive studies on microbial diversity of respiratory infection in cattle to design effective control strategy.

scholarly journals Phenotypic and Molecular Characterization of the Capsular Serotypes of Pasteurella Multocida Isolates From Bovine Respiratory Disease Cases in Ethiopia

2020 ◽  
Author(s):  
Mirtneh Akalu Yilma ◽  
Murthy Bhadra Vemulapati ◽  
Takele Abayneh Tefera ◽  
Martha Yami ◽  
Teferi Degefa Negi ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Pasteurella Multocida ◽  
Bovine Respiratory Disease ◽  
High Sensitivity ◽  
Penicillin G ◽  
Effective Control ◽  
Nasopharyngeal Swab ◽  
Limited Information ◽  
Tissue Samples ◽  
Adult Cattle

Abstract Background: Pasteurella multocida is a heterogeneous species and opportunistic pathogen that causes bovine respiratory disease. This disease is one of an economically important disease in Ethiopia. Losses due to mortality and associated expenses are estimated to be higher in the country. Studies revealed that limited information is available regarding the capsular types, genotypes, and antimicrobial sensitivity of P. multocida isolates circulating in the country. This suggests, further molecular advances to understand the etiological diversity of the pathogens representing severe threats to the cattle population. Results: Bacteriological analysis of nasopharyngeal swab and pneumonic lung tissue samples collected from a total of 400 samples revealed isolation of 61 (15.25%) P. multocida subspecies multocida. 35 (20.59%) were isolated from calves and 26 (11.30%) from adult cattle. Molecular analysis using PCR assay targeting KMT1 gene (~460 bp) amplification was shown in all presumptive isolates. Capsular typing also confirmed the presence of serogroup A (hyaD-hyaC) gene (~1044 bp) and serogroup D (dcbF) gene (~657 bp) from 56 (91.80%) and 5 (8.20%) isolates, respectively. The biovar typing identified 48 (78.69%) isolates of biovar 3. Phylogenetic analysis based on the hyaD-hyaC gene nucleotide sequences showed that P. multocida serogroup A are genetically closely related to the different P. multocida isolates retrieved from the GenBank. Antibiogram of isolates revealed high sensitivity towards chloramphenicol (100%), amoxicillin (95.08%), and amikacin (90.16%). Resistance was observed in cloxacillin (88.52%) and Penicillin-G (77.05%). Conclusions: In the present study, P. multocida capsular type A biovar 3 was predominantly isolated from pneumonic cases in cattle in the study areas. The finding provides useful information for further research and choice of antimicrobials for treatment. Hence, further comprehensive molecular epidemiological investigation is proposed covering wider areas of the country to identify pathogens associated with BRD for an effective control strategy.

scholarly journals Isolation and Molecular Identification of Foot and Mouth Disease Virus Circulating Around Central Ethiopia

2021 ◽  
Author(s):  
Bisratgebriel Tesfaye Muchie ◽  
Ashenafi Kiros Wubshet ◽  
Aklilu Feleke Haile ◽  
Hayelom Michael Deyo ◽  
Kazimierz Tarasiuk ◽  
...  
Keyword(s):  
Addis Ababa ◽  
Foot And Mouth Disease ◽  
Cross Sectional Study ◽  
Effective Control ◽  
Limited Information ◽  
National Veterinary Institute ◽  
Cross Sectional ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Serotype Identification

Abstract Background: Foot and mount disease (FMD) is a highly contagious, economically and politically significant transboundary animal disease which specifically affects all cloven-hoofed animals; cattle, pig, goat, sheep and many wild artiodactyls. Five of the seven (O, A, C, SAT1, SAT2, SAT3 and Asia 1) serotypes of FMD virus (O, A, C, SAT1, SAT2) are endemic in Ethiopia; however, limited information on the current FMDV status and the circulating serotype is available in the country. Therefore, this study was conduct to isolate and molecularly identify the FMD viruses using a panel of virological detection assays. Methods: An outbreak-based cross-sectional study was conducted in Addis Ababa and Bishoftu during 2013 and 2014 to isolate and to molecularly identify the circulating serotype of FMDV. A total of 20 samples were collected from clinically infected cattle and pigs during the outbreak and virus isolation and molecular serotype identification was carried out at the National Veterinary Institute (NVI), Bishoftu Ethiopia. Cells were monitored for cytopathic effects (CPE) daily and frozen when CPE were developed. Serotyping of FMD viruses were made by applying classical PCR from cell cultures showing CPE.Results: All tested samples showed cytopathic effect (CPE) on BHK-21 cell culture and serotype O was identified using PCR. The DNA bands were visualized to the expected sizes.Conclusions: Based on these result, a continuous understanding of the molecular epidemiology of the disease along with the proper vaccination matching for the circulating serotype; O is critical for implementation effective control and prevention programs eventually for the eradication of the disease.

scholarly journals First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia

2021 ◽  
Vol 14 (4) ◽  
pp. 865-872
Author(s):  
Héctor Contreras ◽  
Adriana Vallejo ◽  
Salim Mattar ◽  
Luis Ruiz ◽  
Camilo Guzmán ◽  
...  
Keyword(s):  
Sampling Method ◽  
Clinical Signs ◽  
Cross Sectional Study ◽  
Fish Farms ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Polymerase Chain ◽  
Virus Emergence ◽  
The Brain ◽  
Sanger Method

Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the presence of TiLV in some fish farms in the department of Córdoba. Materials and Methods: A descriptive cross-sectional study was conducted in seven farms using a non-random sampling method from July 2016 to December 2017. A total of 66 fish, including 33 healthy fish and 33 fish with clinical signs, were caught, from which 178 tissue samples of spleen, liver, and brain were collected. RNA was extracted from each organ using TRIzol®. cDNA was synthesized using a retrotranscriptase and a universal amplification primer. The polymerase chain reaction was performed using primers specific to TiLV, in which the primers were amplified in a 491 bp region in segment 3 of TiLV, and the amplicons were sequenced using the Sanger method. Results: Of the seven farms surveyed, 3 (42.85%) had TiLV in the collected fish. Of the 66 collected fish, 18 (27.27%) were infected with TiLV. The virus was detected in the brain (64.3%, 18/28), spleen (61.9%, 13/21), and liver (35.7%, 10/28). The sequences were recorded in GenBank with the codes MH338228, MH350845, and MH350846 . Nucleotide homology analyses revealed that this study's circulating strains exhibited 97% identity with the Israeli strain (GenBank KU751816.1). Conclusion: This is the first official report of TiLV in the department of Córdoba, Colombia. The circulating strains detected in this study exhibited 97% identity with the Israeli strain.

scholarly journals Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus (PRRSv) Isolated From Slaughtered Pigs in Northern Uganda

2021 ◽  
Author(s):  
Peter Oba ◽  
Michel M. Dione ◽  
Joseph Erume ◽  
Barbara Wieland ◽  
Christine Mutisya ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Respiratory Syndrome Virus ◽  
Northern Uganda ◽  
Lung Pathology ◽  
Cross Sectional ◽  
Tissue Samples ◽  
Slaughtered Pigs

Abstract Background A cross sectional study was conducted to determine genotypes of porcine reproductive and respiratory syndrome virus (PRRSv) isolated from slaughtered pigs in Lira district, northern Uganda. The study was conducted from March to September 2019 in three selected slaughter slabs. Pigs brought for slaughter were randomly sampled. At necropsy, lungs were extracted from the thoracic cavity and examined for pneumonic lesions. Seventy-three (73) pigs with gross lung lesions were sampled, from which one hundred and one (101) tissue samples were taken. A real-time reverse transcriptase PCR (RT-qPCR) was used to characterize PRRSv genotypes. Results A total of 20 samples tested positive for PRRSv. The respective prevalence of PRRSv type 1 and type 2 were 24.65% (n = 18) and 2.73% (n = 2) respectively. Of the pigs sampled (n = 73), only two pigs, 2.73% (n = 2) tested positive to both strains. The likelihood of PRRSv detection decreased with pig age, but increased with gross pneumonic pathology. Conclusions This study demonstrated dual circulation of both genotypes in northern Uganda. The association between PRRSv and lung pathology suggests that it may be an important cause of lung disease in pigs in Uganda and hence loss of production. This calls for further investigations on potential economic impacts of PRRSv on pig productivity. These findings contribute to discussions about the need of surveillance and possible vaccination strategies against PRRSv in Uganda.

Genomic, Metagenomic and Transcriptomic Characterization of the Clinical Forms of COVID-19: A Comparative Cross-Sectional Study

2020 ◽  
Author(s):  
Christophe Rodriguez ◽  
Nicolas de Prost ◽  
Slim Fourati ◽  
Claudie Lamoureux ◽  
Guillaume Gricourt ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Clinical Forms

scholarly journals A cross-sectional study reporting concussion exposure, assessment and management in Western Australian general practice

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Elizabeth Thomas ◽  
HuiJun Chih ◽  
Belinda Gabbe ◽  
Melinda Fitzgerald ◽  
Gill Cowen
Keyword(s):  
General Practice ◽  
Cross Sectional Study ◽  
Limited Information ◽  
Sectional Study ◽  
Cross Sectional ◽  
Usual Practice ◽  
Exact Test ◽  
Concussion Management ◽  
Practice Knowledge ◽  
Western Australian

Abstract Background General Practitioners (GPs) may be called upon to assess patients who have sustained a concussion despite limited information being available at this assessment. Information relating to how concussion is actually being assessed and managed in General Practice is scarce. This study aimed to identify characteristics of current Western Australian (WA) GP exposure to patients with concussion, factors associated with GPs’ knowledge of concussion, confidence of GPs in diagnosing and managing patients with concussion, typical referral practices and familiarity of GPs with guidelines. Methods In this cross-sectional study, GPs in WA were recruited via the RACGP WA newsletter and shareGP and the consented GPs completed an electronic survey. Associations were performed using Chi-squared tests or Fisher’s Exact test. Results Sixty-six GPs in WA responded to the survey (response rate = 1.7%). Demographics, usual practice, knowledge, confidence, identification of prolonged recovery as well as guideline and resource awareness of GPs who practised in regional and metropolitan areas were comparable (p > 0.05). Characteristics of GPs were similar between those who identified all symptoms of concussion and distractors correctly and those who did not (p > 0.05). However, 84% of the respondents who had never heard of concussion guidelines were less likely to answer all symptoms and distractors correctly (p = 0.039). Whilst 78% of the GPs who were confident in their diagnoses had heard of guidelines (p = 0.029), confidence in managing concussion was not significantly associated with GPs exposure to guidelines. It should be noted that none of the respondents correctly identified signs of concussion and excluded the distractors. Conclusions Knowledge surrounding concussion guidelines, diagnosis and management varied across GPs in WA. Promotion of available concussion guidelines may assist GPs who lack confidence in making a diagnosis. The lack of association between GPs exposure to guidelines and confidence managing concussion highlights that concussion management may be an area where GPs could benefit from additional education and support.

Evaluation of Supratentorial Brain Neoplasms - A Cross Sectional Study from a Tertiary Care Hospital, Surendranagar, Gujarat

2021 ◽  
Vol 8 (26) ◽  
pp. 2253-2259
Author(s):  
Mohit B. Gor ◽  
Kuldeepsinh D. Mori ◽  
Mehul N. Bavishi ◽  
Nirmala C. Chudasama ◽  
Jaypalsinh D. Barad ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Brain Tumours ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Resonance Imaging ◽  
Cross Sectional ◽  
Multiplanar Imaging ◽  
Higher Sensitivity

BACKGROUND Brain tumours represents 1.7 % of all cancers and contributes 1.8 % of all cancer deaths. Of all the brain tumours, 80 % are supratentorial.1 Magnetic resonance imaging (MRI) is an important modality, having higher sensitivity for detecting intracranial pathology. Multiplanar imaging is possible with MRI which helps in detection, localization and characterization of the lesion. MRI examination has helped in early diagnosis, accurate localization of the tumour, with prompt initiation of appropriate medical or surgical therapy. Recent advances like magnetic resonance (MR) spectroscopy, MR fluoroscopy with stereotactic guided biopsy have revolutionized the role of MRI in study of intracranial tumours. METHODS A cross sectional study of 75 patients was done by Siemens Essenza 1.5T MRI from June 2018 to June 2020 using dedicated head coil. RESULTS In our study, most of the patients were between 31 - 60 years of age (39 % of all patients) with the largest group between 31 - 40 years (20 % of all patients). Out of 75 cases of supratentorial neoplasms, 47 (72 %) were intra-axial lesions, 15 (23 %) were extra-axial lesions and 3 (5 %) were intra ventricular lesions. In this study, 69 % of the lesions were solitary and 31 % were multiple. Most of patients (89 %) showed some enhancement, most common being heterogeneous/inhomogeneous enhancement. Commonest type of supratentorial neoplasms were metastases comprising 29 % of this study. CONCLUSIONS Magnetic resonance imaging is an important modality, having higher sensitivity for detecting intracranial pathology. Multiplanar imaging is possible with MRI which helps in early detection, localization and characterization of the lesion. Metastasis is the most common supratentorial tumour in this study followed by meningioma. Intra-axial tumours are more common than extra-axial tumours in this study. KEYWORDS MRI, Metastasis, Glioma, Meningioma

scholarly journals Cross-Sectional Study Reveals High Prevalence of Clostridium difficile Non-PCR Ribotype 078 Strains in Australian Veal Calves at Slaughter

2013 ◽  
Vol 79 (8) ◽  
pp. 2630-2635 ◽  
Author(s):  
Daniel R. Knight ◽  
Sara Thean ◽  
Papanin Putsathit ◽  
Stan Fenwick ◽  
Thomas V. Riley
Keyword(s):  
Clostridium Difficile ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Adult Cattle ◽  
Content Type ◽  
Retail Meats ◽  
High Prevalence ◽  
Pcr Ribotyping ◽  
Ribotype 078

ABSTRACTRecent reports in North America and Europe ofClostridium difficilebeing isolated from livestock and retail meats of bovine origin have raised concerns about the risk to public health. To assess the situation in Australia, we investigated the prevalence and genetic diversity ofC. difficilein adult cattle and calves at slaughter. Carcass washings, gastrointestinal contents, and feces were collected from abattoirs across five Australian states. Selective culture, toxin profiling, and PCR ribotyping were performed. The prevalence ofC. difficilewas 56% (203/360 samples) in feces from <7-day-old calves, 3.8% (1/26) in 2- to 6-month-old calves, and 1.8% (5/280) in adult cattle. Three PCR ribotypes (RTs), RT127, RT033, and RT126, predominated in <7-day-old calves and comprised 77.8% (158/203 samples) of isolates. RT056, which has not been reported in cattle before, was found in 16 <7-day-old calves (7.7%). Surprisingly, RT078 strains, which dominate production animal carriage studies in the Northern Hemisphere, were not isolated.

scholarly journals Turkish Midwifery Students’ Fear of Delivery Process

2019 ◽  
Vol 8 (3) ◽  
pp. 121-127
Author(s):  
Neslihan Keser Özcan ◽  
Nur Elçin Boyacıoğlu ◽  
Neriman Güdücü ◽  
Seda Göncü Serhatlıoğlu ◽  
Emine Yıldırım ◽  
...  
Keyword(s):  
Sampling Method ◽  
Cross Sectional Study ◽  
Normal Vaginal Delivery ◽  
Decision Making Process ◽  
Test Results ◽  
Cross Sectional ◽  
Wrong Decision ◽  
Mean Values ◽  
Breech Delivery ◽  
Spearman Correlation Test

Introduction: Fear affects a person’s decision-making process and causes a midwife to make a wrong decision about normal vaginal delivery and cesarean delivery, so research was carried out in order to determine the fields related to delivering process in which students experience the most fear and feel sufficient. Methods: A descriptive/cross-sectional study was performed between September and October 2017. The sample consisted of 732 students selected with the convenience maximum variation sampling method from six universities studying in the third and fourth years of a midwifery program. The study measured data collected with a questionnaire entitled “Midwives’ Fear of Delivery Process”. The data were analyzed in with the SPSS for Windows using percentage and mean values and Spearman correlation test. Results: In general, students are more afraid of practices with which they say they have more experience such as vaginal palpation (3.30 (0.884), rs=0.131, P*lt;0.001), and they are less afraid of practices (interventional) with which they have less experience such as breech delivery (1.70 (0.915), rs=0.048, P=0.197) or no experience at all and of cases in which they can get hurt. The students feel most sufficient when performing prenatal and postnatal practices. Conclusion: The results of our study parallel the conclusion in the literature that students are afraid of the fields that they have practiced most. Before clinical practice, instructors can determine students’ fears and deficiencies in relation to procedures and areas of practice and can develop approaches for alleviating their fears and weaknesses.

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