Estimation of Biological Toxicity by Copper oxychloride on Pisum sativum L. and Vigna radiata L.
Background: This research work describes about toxicity estimation of commonly used fungicide copper oxychloride using Pisum sativum L. and Vigna radiata L. as a bioindicator. Methods: The seeds of P. sativum and V. radiata were treated with different concentrations of fungicide. Biological toxicity measured by seed germination percentage, R/P ratio, total leaf chlorophyll, total leaf proline, total seed protein, mitotic index, abnormality index and mitotic inhibition. Result: Linear regression analysis showed that seed germination percentage, total seed protein, abnormality index and mitotic inhibition show positive correlation with increasing concentration of fungicide copper oxychloride. The R/P ratio of 4th and 8th days, mitotic index, total leaf chlorophyll and leaf proline have negative correlation along increasing concentration of fungicide. However total leaf proline of V. radiata at 2% concentration of fungicide is abruptly higher than control and other concentrations. The application of copper oxychloride at lower concentration can be used as a safe fungicide.