Independent and interactive effects of waterlogging and salinity on carbohydrate metabolism and root anatomy in pigeonpea genotypes at different growth stages

2017 ◽  
Vol 51 (03) ◽  
Author(s):  
Savita Duhan ◽  
Sunita Sheokand ◽  
Anita Kumari ◽  
Nidhi Sharma

Four pigeon pea genotypes (ICPH-2431, PARAS, UPAS-120, H09-33) were raised in polythene bags filled with half kg soil + FYM manure mixture. Waterlogging, salinity and waterlogging + salinity treatments were given for 8 days and observations were recorded 1 day after removal from treatment in 20 and 40 day old plants. Significant decline in percent survival was observed under waterlogging and combined treatment in 20 and 40 day old plants. Decline in total sugar content and non reducing sugar content was observed under all three treatments in 20 and 40 day old plant leaves. In roots, an initial increase in total soluble sugar content was observed with waterlogging and salinity treatment. Non reducing sugar content also decreased under all the three treatments. An increase in sucrose synthase and alcohol dehydrogenase activity under all three treatments and formation of aerenchyma in roots under waterlogging and combined treatment was also observed. Waterlogging and salinity in combination was more deleterious compared to alone treatments. Roots were more sensitive to all the stresses as compared to leaves. ICPH performed best among all the genotypes.

2021 ◽  
Vol 9 (8) ◽  
pp. 215-225
Author(s):  
Ulysse Ayihaou Daa-Kpode ◽  
Patrice Hodonou Avogbe ◽  
Gustave Djedatin ◽  
Daniel Datchiglo ◽  
Aristide Bakpe ◽  
...  

Coconut water is highly consumed for refreshment due to its nutritional value. However, its nutritional value varies according to the ecotype and stage of maturity of ecotype. In order to identify best ecotypes with high nutritious value, a physico-chemical composition of ten (10) ecotypes was performed at earlier stage of maturity (3 months). The measured parameters were: coconut height, mass of water, total sugar content, soluble sugar content, protein, titratable acidity and pH.  The obtained results showed that all parameters vary from one ecotype to another. All coconut ecotypes contain water slightly acid with a pH value less than 7.  Furthermore, there is no relationship between physical and biochemical parameters. However, within characterized ecotypes, Ecotype_2, appeared to have the best biochemical composition with a significant amount of water. This study provided important informations related to physico-chemical characteristics of coconuts ecotypes found in the coastal zone.


1970 ◽  
Vol 16 ◽  
pp. 95-99 ◽  
Author(s):  
Md Rezaul Karim ◽  
MMH Khan ◽  
Md Salim Uddin ◽  
NK Sana ◽  
F Nikkon ◽  
...  

Significant differences were found in sugar content and carbohydrate splitting enzyme activities in tubers of ten indigenous potato varieties at harvesting and after keeping at cold storage. The activities of invertase, amylase, β-galactosidase and cellulase in all varieties were found to be increased by 2-12, 1.2-4, 1.9-4.5, and 1.1-3.7 folds, respectively from harvesting to cold stored potatoes. The amount of starch and sucrose were found to be decreased by 1.15-2.8 and 1.02-1.4 folds, respectively from harvesting to cold stored in all varieties. Total soluble sugar and reducing sugar contents in potatoes were increased by 1.02-1.4 and 4-11 folds, respectively from harvesting to cold stored in all varieties of potatoes. The amount of reducing sugar increased in cold stored potatoes due to the increased activities of carbohydrate splitting enzymes. Key words: Potatoes, Indigenous, Carbohydrate splitting enzymes, Sugars. DOI:10.3329/jbs.v16i0.3748 J. bio-sci. 16: 95-99, 2008


2016 ◽  
Vol 2 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Md Moniruzzaman Sohag Howlader ◽  
Sheikh Rashel Ahmed ◽  
Khadizatul Kubra ◽  
Md Khairul Hassan Bhuiyan

The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.Asian J. Med. Biol. Res. March 2016, 2(1): 121-130


1961 ◽  
Vol 1 (3) ◽  
pp. 153 ◽  
Author(s):  
JF Melvin ◽  
MA Sutherland

The effect of shading for 64 hours before cutting on the soluble sugar contents of short rotation ryegrass has been determined at weekly intervals during growth. From grass with five to six leaves emergent to the stage of growth usually accepted for hay-making, the average reduction in the total sugar content of the shaded plants was about 30 per cent, made up of decreases in sucrose (12 per cent), oligosaccharides (5 per cent) and fructosans (13 per cent).


2017 ◽  
Vol 8 (1) ◽  
pp. 99 ◽  
Author(s):  
Cíntia Martins De Oliveira ◽  
Welson Lima Simões ◽  
Paulo Roberto Coelho Lopes ◽  
José Bezerra Da Silva ◽  
Emanoel Jurema Araújo ◽  
...  

The objective of this study was to evaluate the effect of different irrigation levels on flowering, fruit set, carbohydrate and protein content and gas exchange, of apple varietiesin the Brazilian semiarid region. The experimental design was a randomized block with split plots with five replications and four plot irrigation levels (60, 80, 100, and 120% of the reference evapotranspiration (ETo)) and the subplots with two cultivars (Juliet and Princess). As a result, it was found that the number of flowers (NFL), fruits (NFR), shoot percentage (SP) and fruit set (FS) were higher in cv. Juliet. The number of buds (NB) and the fertility index (FI) were higher in cv. Princess. With regard to metabolites, reducing sugar content (RS) had no significant effect among cultivars. The non-reducing sugar content (NRS), total soluble sugar (TSS) and total soluble proteins (TSP) were higher in cv. Princess. As for irrigation depths, TSP, sprouting, flowering and fruit set increased along with the amount of water applied, while the RS, NRS and TSS contents had the opposite behavior.


2017 ◽  
Vol 17 (1) ◽  
pp. 37 ◽  
Author(s):  
Zvezda Bogevska ◽  
Rukie Agic ◽  
Gordana Popsimonova ◽  
Margarita Davitkovska ◽  
Igor Iljovski

In Macedonia, sweet onion landrace ‘buchinska arshlama’ is traditionally stored during winter period from October until April. The objective of this research was to determine the content of reducing sugar and total sugar in onion bulbs during storage period in a traditional way and in cold rooms. Every month, samples were taken for chemical analysis. The results were statistically processed and tested with t-test. The average reducing sugar content in onion stored in a traditional way was 4.8%, while the average reducing sugar content in onion stored in cold rooms was 4.6%. The average total sugar content in onion stored in a traditional way was 6.0%, while the average total sugar content in onion stored in a cold room was 5.6%. These results showed that onion `buchinska arshlama` can be successfully stored in a traditional way until the beginning of February or longer in cold rooms.


Author(s):  
Muhamad Yusuf Hasan ◽  
Mohd Ali Hassan ◽  
Mohd Noriznan Mokhtar ◽  
Azni Idris ◽  
Yoshihito Shirai ◽  
...  

The main objective of this work was to investigate the effects of the controlled periodic addition of anaerobic sludge during composting to increase amount of microbial DNA, which appears to be correlated to soluble sugar content which may relate to rate of lignocellulosic degradation. In this study, the composting of pressed-shredded oil palm empty fruit bunch with the periodic addition of palm oil mill effluent anaerobic sludge for moisture control in a newly designed in-vessel type composter was carried out. A control experiment was also conducted over the same period but with the periodic addition of water for moisture control instead of the anaerobic sludge. The lignocellulosic composition and the reducing sugar content were determined via fibre analysis and the spectrophotometric method respectively. The bacterial profile throughout the composting process was quantified by using qPCR. The growth of bacteria reached its peak at 48°C and the degradation of lignocellulose was highest during the thermophilic stage. The highest content of reducing sugar coincided with the highest degradation rate of lignocellulose and the highest DNA copy number during the thermophilic stage. Under the controlled experimental condition of increasing the microbial community, the composting was accelerated to 2.07% OM degradation per day compared to the water addition control at 0.60% OM per day.


2021 ◽  
Vol 924 (1) ◽  
pp. 012012
Author(s):  
B Susilo ◽  
S M Sutan ◽  
Y Hendrawan ◽  
R Damayanti

Abstract Red dragon fruit (Hylocereus costaricensis) is a famous fruit because of its high nutritional content. Although the product diversification of red dragon fruit is quite a lot, however, this product has limited information as a syrup product. The evaporation process for making syrup at the traditional level is usually carried out at high temperatures which results in the high destruction of nutritional content and reduces the quality of syrup. The study of the evaporation process at low temperature and low pressure using a double jacket vacuum evaporator for manufacturing red dragon fruit syrup was conducted in this research in order to improve product quality. The aims of this study are to evaluate the effect of temperature process and vacuum pressure on the quality of red dragon fruit (H.costaricensis) syrup using a double jacket vacuum evaporator and to know the optimum temperature process and vacuum pressure to producing red dragon fruit sirup with higher content of vitamin C. The double jacket evaporator operates at a pressure lower than atmospheric pressure so that there were 9 treatment combinations. The result showed the temperature gave a highly significant effect, especially on vitamin C. It also gave on moisture content and reducing sugar. The temperature gave no significant effect on total sugar content. Whereas, the pressure gave a significant on moisture content, but not gave a significant on vitamin C, reducing sugar content and total sugar content. However, the best treatment for optimum vitamin C is 50 0C/-60 cmHg with 8.75 mg/100 ml.


2000 ◽  
Vol 51 (8) ◽  
pp. 1023 ◽  
Author(s):  
Altaf Ahmad ◽  
Ishrat Khan ◽  
M. Z. Abdin

The effect of sulfur (S) fertilisation on oil accumulation, acetyl-CoA concentration, and activity of acetyl-CoA carboxylase (EC 6.4.1.2) was determined in the developing seeds of rapeseed (Brassica campestris L. cv. Pusa Gold) grown in the field with and without S. The period between 14 and 35 days after flowering (DAF) was identified as the active period of oil accumulation in the developing seeds of rapeseed. The accumulation of oil was preceded by a marked rise in acetyl-CoA carboxylase activity and acetyl-CoA concentration, which declined rapidly when oil accumulation reached a plateau. Starch and soluble sugar content decreased, while protein content increased during the period of active oil accumulation in the developing seeds (i.e. 14–35 DAF). Sulfur fertilisation significantly (P < 0.05) enhanced the oil accumulation in the developing seeds at all the growth stages except at 7 DAF. The increase in the oil content was 13.0–52.0% with S fertilisation over the control treatment. Sulfur fertilisation also increased acetyl-CoA concentration, acetyl-CoA carboxylase activity, and soluble protein, sugar, and starch content in the developing seeds. It is suggested that the increase in the oil content with S fertilisation may be associated with increases in acetyl-CoA carboxylase activity through the enhancement of acetyl-CoA concentration. Further, the increased sugar content due to S fertilisation provided enough carbon sources for oil biosynthesis.


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