Ameliorative effect of thiourea priming on germination characteristics of mungbean (Vigna radiata L.) under water and salinity stress

Author(s):  
Shalini Jhanji ◽  
Madhu Dhingra

The germination behaviour of unsoaked, hydroprimed and thiourea primed seeds (TU,750, 1000 and 1250 ppm) in water , polyethylene glycol (PEG, -0.2 and -0.4 MPa ) and NaCl (30 and 50 mM) was investigated. The percent germination and seedling growth of TU primed seeds was best compared to other treatments under stressed conditions. The root/shoot ratio increased with stress in all seedlings and vice versa for seedling vigour index. TU primed seeds exhibited the highest tolerance index (85) under - 0.2 MPa PEG treatment and 40 tolerance index under 30mM salinity stress. Priming with thiourea @1000 ppm was the most effective in ameliorating water and salinity stress.

2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.


2021 ◽  
Vol 17 (2) ◽  
pp. 509-514
Author(s):  
A. Krishna ◽  
Jitendra Kumar S. Hilli

Soapnut (Sapinduas emarginatus) is one of the most primitive precious useful plants since ancient times. This plant was domesticated due to its multifarious usefulness. The study was conducted in College of Forestry, Sirsi during 2019-20 to know the influence of post scarification storage treatments on seed germination and quality in Sapinduas emarginatus. The seeds were collected from in and around Sirsi area. In soap nut, the basic problem is poor seed germination due to hard seed coat. The seeds were treated with concentrated H2So4 for 12min and stored under laboratory condition for six months. At monthly interval, seed samples were drawn and evaluated for post scarification germination. The germination behaviour remarkably influenced due to scarification and without scarification during experimentation. The untreated seeds recorded the maximum germination (68%) compared to scarified seeds (22.85%).Speed of germination was highest in seed without scarification treatment (2.7) compared to scarified seeds. Mean daily germination was highest in without scarified seeds (1.04) and lowest in the scarified seeds (0.32). Peak value was maximum in without scarified seeds (0.50) compared to scarified seeds (0.22). Seedling vigour index was significantly highest in without scarified seeds (1290) compared to scarified seeds (455). In general, scarified seeds with conc. H2SO4 for 12 min affected seed germination due high conc. H2SO4 because of chemical residues remaining in seed that affects the embryo. As the advancement in storage period the seed coat may lose the integrity so, germination was improved in without scarified seeds.


Agropedology ◽  
2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Ranbeer Singh Chauhan ◽  
◽  
J.S. Chauhan ◽  
A.S. Rawat ◽  
Dinesh Singh Rawat ◽  
...  

Germination ability of seeds varies significantly amongst different varieties of same crop when exposed to the stress. Paddy (Oryza sativa L.) is known to be highly sensitive to salinity during germination and young seedling stages. Present study was aimed to observe the effects of six salinity concentrations (0%, 0.1%, 0.5%, 1%, 1.5% and 2%) on the germination behaviour of two paddy landraces (Chenaphool and Gyasu), frequently grown in Chakrata area of district Dehradun, Uttarakhand. Salinity (>0.1%) was inversely related to final germination percentage, germination energy, plumule dry weight, plumule length, radical dry weight, radical length and speed of germination. Chenaphool landrace showed higher germination and growth (plumule and radical) at 0% salinity (control), while Gyasu landrace at 0.1% salinity level.Dry weight percentage reduction increased with increasing salinity level, while negative correlation was observed between salinity and salt tolerance index and seed vigour index for both the varieties. The study concluded that the long grained, irrigated landrace Chenaphool was more sensitive to salinity in comparison to short grained, non-irrigated landrace Gyassu. Lower salinity conditions have no adverse effects on the germination behaviour of Gyasu landrace.


2015 ◽  
Vol 9 (4) ◽  
pp. 10-15
Author(s):  
Hemanth Kumar N K ◽  
Shobha Jagannath

Herbicides are the most flourishing weed control knowledge eternally developed. To appraise the possessions of herbicides on maize, a study was carried out to evaluate the effect of alachlor on different maize cultivars on germination and early growth parameters. The  studies were carried out in hydroponic conditions supplemented with different concentrations (1.0, 2.5 5.0, 7.5 and 10.0 ppm) of alachlor. All the three cultivars of maize were found to be significantly affected with increasing herbicide concentration. Percent germination, vigour index, tolerance index, fresh and dry weight, plumule and radicle length were found to be decreased as the herbicide concentration increased, however the dry weight increased slightly at higher concentration when compared to other concentrations. The percent toxicity increased as the concentration of herbicide increased. The current study clearly implies that higher concentration of alachlor is toxic to maize, resulted in a decline in seed germination and early growth parameters.DOI: http://dx.doi.org/10.3126/ijls.v9i4.12638


2021 ◽  
Vol 49 (3) ◽  
pp. 12063
Author(s):  
Ali SHOKOUHIAN ◽  
Heshmat OMIDI

Seed priming has proved to be an effective method in imparting stress tolerance to plants using natural and/or synthetic compounds to treat the seeds before germination. The present study was designed to investigate the physiological mechanism of seed priming with ZnSO4 (osmopriming) and distilled water (hydropriming) on sugar beet genotypes (‘Shokofa’, ‘Sina’, ‘Paya’, ‘Turbata’, and ‘Aria’) germination indices, seedling growth parameters, and biochemical properties under salinity stress (0, 2, 5, and 12 dS/m NaCl). A significant reduction in germination percentage (33.23%), germination rate (77.2%), chlorophyll a, b, and total contents (43.9, 31.9, and 39.9%, respectively) while, a significant increase in radical, plumule, and seedling length (57.1, 44.4, and 51.2%, respectively), seedling vigour index (48.9%), superoxide dismutase activity (61.3%), proline (54.0%) and sugar (56.3%) contents were achieved at 12 dS/m NaCl in compared to the control treatment. Seed hydropriming and osmopriming caused significant improvements in photosynthetic pigments, antioxidant enzyme activity, and proline content reflected in high germination percentage and rate as well as seedling vigour index and reduced mean germination time under salinity. ‘Paya’ and ‘Aria’ genotypes had a superiority according to the germination percentage and seedling vigour index, respectively. The hydropriming of ‘Paya’ genotype resulted in the highest germination percentage (95%) under high level of salinity (12 dS/m) which 11.84% increase compared to the control treatment. Hydropriming of ‘Sina’ seeds showed the highest chlorophyll a and total, and carotenoids under non-stress conditions (22.89, 31.65, and 2116.6 µg/g FW). Also, hydropriming by increases chlorophyll b content led to the modulation of the negative effects of high salinity stress (12 dS/m). In conclusion, different seed priming treatments in sugar beet seeds improved the salinity tolerance by physiological characteristics nonetheless hydropriming was the most effective treatment to get higher germination indices in ‘Paya’ and ‘Aria’ genotypes.


2014 ◽  
Vol 19 ◽  
pp. 1-5
Author(s):  
Meenu Gehlot ◽  
Pawan K. Kasera

Phyllanthus amarus (Bhui-Aanwalaa: Family: Euphorbiaceae) is considered to be the most important medicinal plant of the Indian Thar desert. The present article deals with its seed morphological parameters, seed output, reproductive capacity (RC), germination behavior, seedling growth, root: shoot ratio (R/S ratio), germination value (GV) and vigour index (VI) in fresh and one-year-old seeds under controlled laboratory conditions. Results revealed that 73.33 and 60% germination was observed in fresh and one-year-old seeds as compared to the control (20% in fresh; while 36.67% in one-year-old seeds) after providing mechanical + conc. H2SO4 scarification for 2 and 1 min, respectively. The maximum VI and GV were obtained in mechanical + conc. H2SO4 scarification for 2 min pretreatments in fresh seeds.DOI: http://dx.doi.org/10.3126/eco.v19i0.9848EcoprintAn International Journal of EcologyVol. 19, 2012Page: 1-5Uploaded date: 2/14/2014


2019 ◽  
Vol 26 (1) ◽  
pp. 23-25
Author(s):  
Pitambar Singh Negi ◽  
◽  
Sandeep Sharma ◽  

An experiment was conducted to study the effect of pre-sowing treatments on germination of Abies spectabilis commonly called as “Talispatra” - an important high level conifer of Western Himalayan region. The seeds of Abies spectabilis collected from Kalabagh near Churdhar area of Shimla district (H.P.) were given different pre-sowing treatments of cold moist stratification in sand for 1 to 4 weeks and control (untreated seeds) to study their effect on germination behaviour and seedling vigour index. Significant differences were observed in germination per cent and seedling vigour index of seeds subjected to different pre-sowing treatments. The maximum germination per cent (42.00%) was recorded in seeds treated with cold moist stratification treatment in sand for 1 week followed by 36.00 per cent germination recorded for control (untreated seeds). The minimum germination per cent (19.50%) was recorded in seeds treated with cold moist stratification treatment in sand for 4 weeks. The findings of the present investigation revealed that Abies spectabilis seeds do not possess any dormancy and the poor germination is primarily due to presence of large number of empty seeds.


2020 ◽  
pp. 104-107
Author(s):  
Pooja Bohra ◽  
Ajit Arun Waman ◽  
R. Karthika Devi

Blood fruit is a tropical underutilized species, fruits of which have been identified as source of natural colourant. However, forests are the major source of fruits to native people. In order to conserve the natural population of this species and promote cultivation, nursery techniques need to be standardized. In the present investigation, effect of three substrates viz. vermicompost, sand and soil + vermicompost was studied on seed germination characteristics. Results revealed significant differences among the treatments. Use of vermicompost improved seed germination (69%) significantly over the other substrates used. Though most of the studied seedling growth parameters did not differ among the treatments, significantly highest seedling vigour index (5466.9) was recorded when vermicompost was used as substrate. Considering these findings, use of vermicompost is advisable for germination of blood fruit in nursery.


2020 ◽  
Vol 8 (2) ◽  
pp. 104-107
Author(s):  
Pooja Bohra ◽  
Arun Waman ◽  
R. Karthika Devi

Blood fruit is a tropical underutilized species, fruits of which have been identified as source of natural colourant. However, forests are the major source of fruits to native people. In order to conserve the natural population of this species and promote cultivation, nursery techniques need to be standardized. In the present investigation, effect of three substrates viz. vermicompost, sand and soil + vermicompost was studied on seed germination characteristics. Results revealed significant differences among the treatments. Use of vermicompost improved seed germination (69%) significantly over the other substrates used. Though most of the studied seedling growth parameters did not differ among the treatments, significantly highest seedling vigour index (5466.9) was recorded when vermicompost was used as substrate. Considering these findings, use of vermicompost is advisable for germination of blood fruit in nursery.


2007 ◽  
Vol 17 (3) ◽  
pp. 175-181 ◽  
Author(s):  
Neeru Jain ◽  
Johannes Van Staden

AbstractThe stimulatory role of 3-methyl-2H-furo[2,3-c]pyran-2-one, a smoke-derived butenolide, on germination and post-germination events is well documented. Previous studies have involved germinating seeds in the continuous presence of the compound. However, commercial growers cannot exploit the potential of the butenolide for large-scale production of crops due to limited availability and environmental constraints. The present study was undertaken to assess the potential of the butenolide as a priming agent of tomato (Solanum esculentum Mill.) seeds. Flow cytometry data revealed that butenolide-primed seeds had a higher percentage of nuclei at the 4C stage than water-primed seeds. Emergence of the radicle was much faster in the primed seeds. After 36 h of imbibition, a higher percentage of the butenolide-primed seeds (22%) exhibited radicle emergence compared to the water-primed seeds (12%). While butenolide-primed seeds initially germinated more rapidly than either water-primed or unprimed seeds in a 48-h period, water-imbibed seeds reached a similar germination level as the butenolide-primed seeds by 60 h of incubation. The butenolide-primed seeds produced significantly (P ≤ 0.05) more vigorous seedlings than water-primed seeds or seeds in the continuous presence of either water or butenolide. A gradual decrease in the seedling vigour index was recorded for both water and butenolide-primed seeds with increased seed storage at room temperature. Nevertheless, the vigour index was significantly greater in the butenolide-primed seeds than the water-primed seeds. Vigour indices were significantly (P ≤ 0.05) higher for the butenolide-primed seeds under various stress conditions (salinity, osmoticum or temperature) compared to control or water-primed seeds. Results of the present study suggest that the butenolide is a good seed-priming agent. Additionally, primed seeds retained the promotive effect for a considerable time. This was also the case for tomato seeds under various simulated field stress conditions.


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