scholarly journals Impact of post scarification storage period on seed germination and quality in soapnut (Sapinduas emarginatus)

2021 ◽  
Vol 17 (2) ◽  
pp. 509-514
Author(s):  
A. Krishna ◽  
Jitendra Kumar S. Hilli
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Storage Period ◽  
Monthly Interval ◽  
Seedling Vigour ◽  
Hard Seed ◽  
Chemical Residues ◽  
Poor Seed ◽  
Vigour Index ◽  
Germination Behaviour

Soapnut (Sapinduas emarginatus) is one of the most primitive precious useful plants since ancient times. This plant was domesticated due to its multifarious usefulness. The study was conducted in College of Forestry, Sirsi during 2019-20 to know the influence of post scarification storage treatments on seed germination and quality in Sapinduas emarginatus. The seeds were collected from in and around Sirsi area. In soap nut, the basic problem is poor seed germination due to hard seed coat. The seeds were treated with concentrated H2So4 for 12min and stored under laboratory condition for six months. At monthly interval, seed samples were drawn and evaluated for post scarification germination. The germination behaviour remarkably influenced due to scarification and without scarification during experimentation. The untreated seeds recorded the maximum germination (68%) compared to scarified seeds (22.85%).Speed of germination was highest in seed without scarification treatment (2.7) compared to scarified seeds. Mean daily germination was highest in without scarified seeds (1.04) and lowest in the scarified seeds (0.32). Peak value was maximum in without scarified seeds (0.50) compared to scarified seeds (0.22). Seedling vigour index was significantly highest in without scarified seeds (1290) compared to scarified seeds (455). In general, scarified seeds with conc. H2SO4 for 12 min affected seed germination due high conc. H2SO4 because of chemical residues remaining in seed that affects the embryo. As the advancement in storage period the seed coat may lose the integrity so, germination was improved in without scarified seeds.

In vitro Plant Regeneration of Rauvolfia tetraphylla L.: A Threatened Medicinal Plant

2020 ◽  
Vol 30 (1) ◽  
pp. 33-45
Author(s):  
Rabbi Hoque ◽  
Rita Sarah Borna ◽  
M Imdadul Hoque ◽  
RH Sarker
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Seed Coat ◽  
Root Induction ◽  
Ex Vitro ◽  
Hard Seed ◽  
Poor Seed ◽  
Seed Propagation ◽  
Hard Seed Coat

Poor seed germination is the main obstacle for seed propagation of Rauwolfia tetraphylla L. in nature. The high viability (83.1%) of seeds in nature and the presence of viable embryo indicate that viability is not the only reason behind poor seed germination of this valuable medicinal plant. However, to overcome the problem of poor seed propagation, an efficient protocol has been developed for R. tetraphylla. Among the various treatments the maximum rate of ex vitro seed germination (13.33%) was found when the seeds were treated with 100 ppm of GA3. Response of in vitro germination was found to vary under different conditions. Not only that the rate was much higher compared to ex vitro germination. The rate of seed germination was found to be 78% in MS without PGR (plant growth regulator) supplements, while it was 70 and 78% in cotton bed under light and dark, respectively. It was 80% in incubator at 37ºC. In R. tetraphylla the hard seed coat is regarded as one of the barriers for germination and it can be easily eliminated by removing the hard seed coat. In vitro raised plantlets were reared in nature following proper acclimatization where they produced flowers as well as seeds. Apart from the said investigation, a method for in vitro shoot formation was also developed. Best response (90.7%) towards in vitro shoot regeneration was obtained from nodal segment when they were cultured on MS supplemented with 2.2 mg/l BA and 0.1 mg/l NAA. It took about 10-12 days to initiate shoots. About 9.9 ± 0.87 shoots were obtained per explants and their length was recorded as 2.28 ± 0.21 cm after six weeks. Various concentrations of IBA and NAA were used for in vitro root induction, but the in vitro raised shoots did not produce roots. Plant Tissue Cult. & Biotech. 30(1): 33-45, 2020 (June)

Effect of pre-sowing treatments on seed germination and seedling vigour index of Abies spectabilis (D.Don) spach

2019 ◽  
Vol 26 (1) ◽  
pp. 23-25
Author(s):  
Pitambar Singh Negi ◽  
◽  
Sandeep Sharma ◽  
Keyword(s):  
Seed Germination ◽  
Seedling Vigour ◽  
The Poor ◽  
Western Himalayan Region ◽  
Vigour Index ◽  
Germination Behaviour ◽  
Abies Spectabilis ◽  
High Level ◽  
And Control ◽  
Empty Seeds

An experiment was conducted to study the effect of pre-sowing treatments on germination of Abies spectabilis commonly called as “Talispatra” - an important high level conifer of Western Himalayan region. The seeds of Abies spectabilis collected from Kalabagh near Churdhar area of Shimla district (H.P.) were given different pre-sowing treatments of cold moist stratification in sand for 1 to 4 weeks and control (untreated seeds) to study their effect on germination behaviour and seedling vigour index. Significant differences were observed in germination per cent and seedling vigour index of seeds subjected to different pre-sowing treatments. The maximum germination per cent (42.00%) was recorded in seeds treated with cold moist stratification treatment in sand for 1 week followed by 36.00 per cent germination recorded for control (untreated seeds). The minimum germination per cent (19.50%) was recorded in seeds treated with cold moist stratification treatment in sand for 4 weeks. The findings of the present investigation revealed that Abies spectabilis seeds do not possess any dormancy and the poor germination is primarily due to presence of large number of empty seeds.

scholarly journals Effect of Various Treatments on Seed Germination and Seedling Vigour of Aonla cv. Chakaiya

2020 ◽  
Author(s):  
Satnam Singh ◽  
Amarjeet Kaur
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Time Interval ◽  
Seedling Vigour ◽  
Planting Material ◽  
Poor Seed ◽  
Vigour Index ◽  
Germination And Growth ◽  
Day By Day ◽  
Growth Of Seedlings

Background: Aonla is considered asone of the important indigenous minor fruit crop. Being rich in nutrients the demand of its planting material is increasing day by day which is not fulfilled due to poor seed germination and seedling growth. Therefore there is a dire necessity to standardize the techniques for improving germination and growth of seedlings. The aim of the current study is to overcome the dormancy and to enhance the germination in aonla seeds. Methods: The present investigation was carried out during 2019-2020 under field conditions with the pre soaking application of the aonla seeds with thiourea, GA3, cow urine, sulphuric acid, water and seed stratification for time interval of 12 and 24 hours. In the laboratory the selected samples were analysed morphologically based on shoot, root and biomass traits.Result: Out of the various treatments the application of GA3@ 500 ppm for 24 h have ensured early germination with an increase in seed germination. This treatment also improved the seedling growth with an increase in root formationand vigour index. This study will be useful in boosting the cultivation of aonla which will be beneficial for the growers on commercial basis.

scholarly journals The Influence Of Zinc On Seed Germination And Growth In The First Ontogenetic Stages In The Species Cucumis Melo L.

2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Growth Process ◽  
Lateral Roots ◽  
Tolerance Index ◽  
High Concentration ◽  
Seedling Vigour ◽  
Ontogenetic Stages ◽  
Vigour Index ◽  
Germination And Growth

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.

scholarly journals Effect of Osmotic and Matric Potentials on the Availability of Water for Seed Germination

1971 ◽  
Vol 24 (3) ◽  
pp. 423 ◽  
Author(s):  
JR Mcwilliam ◽  
PJ Phlllips
Keyword(s):  
Soil Moisture ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Soil Water ◽  
Seed Coat ◽  
Moisture Diffusivity ◽  
Matric Potential ◽  
Water Potentials ◽  
Germination Behaviour ◽  
Dry Soil

Under special conditions where soil-moisture diffusivity and seed-soil contact are non-limiting, the osmotic and matric potentials of the substrate were found to be equivalent in their effect on the germination of seeds of ryegrass and dehulled phalaris over a range of water potentials from 0 to -15 bars. However, with intact phalaris seeds it appears that the seed coat constitutes a large resistance to the absorption of soil water, and under these conditions the equivalence between osmotic and matric potential no longer holds, and results of germination under osmotic stress must be used with caution in predicting the germination behaviour of seeds in dry soil.

scholarly journals Fungicidal seed treatment on germination and seedling vigour of lentil var. BINA Masur-3

2017 ◽  
Vol 3 (1) ◽  
pp. 140-144
Author(s):  
A Rokib ◽  
MS Monjil
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Seed Treatment ◽  
Shoot Length ◽  
Seedling Vigour ◽  
Green House ◽  
Seed Pathology ◽  
Vigour Index ◽  
Positive Effect ◽  
Improve Seed Germination

Effectiveness of six fungicides viz., Provax-200 (Carboxin+Thiram), Bavistin DF (Carbendazim), Dithane M-45 (Mancozeb), Secure (Mancozeb+Fenamidone), Antracol (propineb) and Daconil (Chlorothalonil) were evaluated to improve seed germination and seedling vigour of lentil variety BINA Masur-3. The experiment was conducted in the Green House of Seed Pathology Centre, Bangladesh Agricultural University, Mymensingh. Positive effect of seed treatment with fungicides on seed germination and seedling vigour of lentil was recorded. At 20 DAS, increased seed germination over control was observed in treated seeds with Deconil (10.40%) and Antracol (10.00%) followed by Dithane M-45 (7.20%). Seeds treated with Deconil and Dithane M-45 produced seedlings with higher shoot length, root length and seedling vigour. At 20 DAS, higher percent increased vigour index over control was found in Dithane M-45 (24.64%) and Deconil (22.44%), respectively.Asian J. Med. Biol. Res. March 2017, 3(1): 140-144

scholarly journals Seed Germination of selected Taxa from Kachchh Desert, India

2010 ◽  
Vol 2 (2) ◽  
pp. 41-45 ◽  
Author(s):  
Vinay Madhukar RAOLE ◽  
Aruna Girish JOSHI ◽  
Sandhya Kiran GARGE ◽  
Rinku Jitendrakumar DESAI
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
Seed Coat ◽  
Native Species ◽  
Arid Regions ◽  
Conservation Status ◽  
Storage Period ◽  
The Other ◽  
Viable Seeds ◽  
Semi Arid

The district of Kachchh contains many culturally important plants. However, their conservation status is little known due to direct and indirect human activities. This study was undertaken with the aim of contributing to the conservation of the native species of these semi-arid regions through germination trials under laboratory conditions. Mature fruits of ten selected species were collected randomly from the known habitats to obtain viable seeds. These seeds were pre-treated with growth regulators singly or in combination after acid scarification or without scarification. Seeds were found to be dormant due to presence of thick seed coat or due to low level of endogenous hormonal level. Most of these seeds required different storage period to mature. Only seeds of Capparis cartilaginea germinated without treatment while the other species required treatments. Addition of growth regulators has enhanced seed germination in few taxa singly and in some plant cases in combination.

scholarly journals Effect of seed hydro-priming durations on germination and seedling growth of bitter gourd (Momordica charantia)

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255258
Author(s):  
Binod Adhikari ◽  
Pankaj Raj Dhital ◽  
Sambat Ranabhat ◽  
Hari Poudel
Keyword(s):  
Water Uptake ◽  
Seedling Growth ◽  
Block Design ◽  
Cost Effective ◽  
Momordica Charantia ◽  
Bitter Gourd ◽  
Seedling Vigour ◽  
Hard Seed ◽  
Yield And Quality ◽  
Vigour Index

The yield and quality of an annual crop are highly dependent on uniform and rapid germination of the seeds. In case of bitter gourd (Momordica charantia), the germination and field emergence is always a problem as seeds have thick and hard seed coat. Pre-sowing hydro-priming is one of the most suitable, affordable, easily available, and cost-effective techniques in breaking down seed dormancy to enhance germination. Hence, a field experiment was conducted in Surkhet district of Nepal 2020 to assess the effect of different hydro-priming duration on germination and seedling growth of bitter gourd. The experiment was laid out in single factor Randomized Complete Block Design (RCBD) with four replications and seven treatments including different hydro-priming durations (T0: control, T1: 6 hours, T2: 12 hours, T3: 18 hours, T4: 24 hours, T5: 36 hours and T6: 48 hours) of bitter gourd seeds of Palee variety, the most popular variety among the farmers. The highest water uptake and germination were found in 48 hours of seed hydro-primed seeds whereas the lowest water uptake and germination were observed on non-primed seeds. Similarly, the tallest seedling, most vigorous seedling in terms of seedling vigour index I and II was observed in 48 hours hydro-primed seeds followed by 36 hours of seed hydro-priming and shortest seedling and the least vigorous seedling in control. Thus 48 hours of seed hydro-priming was found to be effective for increasing germination and seedling growth in bitter gourd, which needs to be further investigated under large, open-field conditions with different varieties.

scholarly journals Effect of nano based seed treatment insecticides on seed quality in Pigeonpea

2017 ◽  
Vol 9 (2) ◽  
pp. 1226-1235
Author(s):  
B.N. Raghu ◽  
Basave Gowda ◽  
S. N. Vasudevan ◽  
Sangeeta I. Macha ◽  
Sharan Gouda Hiregoudar ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Seed Quality ◽  
Dry Weight ◽  
High Energy ◽  
Seed Kernel ◽  
Neem Seed ◽  
Seedling Vigour ◽  
Vigour Index ◽  
Sweet Flag

A laboratory experiment was conducted to know the effect seed treatment with nano insecticides on seed quality of pigeonpea (Cajanus cajan (L.) Millsp.) cv. TS3R. This study was conducted to evaluate the effect of macro and nano insecticides on seed germination and vigour of Pigeonpea. Different recommended seed treatment insecticides viz, malathion, fenvalerate, emamectine benzoate, thiodicarb, sweet flag and neem seed kernel powder insecticides were synthesized to nano form using high energy planetary ball mill. The Pigeonpea seed were treated with different nano insecticides i.e., 10-90 per cent reduction in actual dosage. Among the different treatments studied, seed treated with nano malathion 50 per cent lesser than normal dosage, fenvalerate 60 per cent lesser, thiodicarb 10 per cent lesser, emamectine benzoate 30 per cent lesser, sweetflag 70 per cent lesser, neem seed kernel powder 40 per cent lesser than actual recommended dosage gave significantly higher seed germination (98.0, 98.67, 98.67, 97.0, 99.0 and 98.67 percent) ,less number of abnormal seedlings (1.0, 0.33, 1.0, 1.0, 1.0 and 0.33 per cent) , shoot length (10.13, 9.00, 11.47, 9.50, 10.90 and 10.87 cm), root length (12.56, 12.93, 12.83, 12.60 11.50 and 13.00 cm), seedling dry weight (85.73, 87.40, 88.47, 87.70, 88.60 and 88.27 g) and seedling vigour index (2223, 2164, 2397, 2143, 2217 and 2354) as compared to untreated seeds and macro insecticides. Therefore, it is very clear that nano based insecticides has a significant (0.1 %) impact on the seed quality improvement.

scholarly journals Screening of efficient phosphate solubilizing fungi from mine soil and effect of phosphofungi on seed germination and vigour index of ground nut (arachis hypogaea L.) and green gram (vigna radiata L.)

2016 ◽  
Vol 4 (2) ◽  
pp. 288
Author(s):  
Shanubhoganahalli Thimmappa Lokesh ◽  
Kudure Jayanna Naveen Kumar ◽  
Basaiah Thippeswamy
Keyword(s):  
Aspergillus Niger ◽  
Seed Germination ◽  
Aspergillus Flavus ◽  
Tricalcium Phosphate ◽  
Mine Soil ◽  
Seedling Vigour ◽  
Fungal Isolates ◽  
Vigour Index ◽  
Phosphate Solubilizing ◽  
Solubilizing Activity

Background: The use of chemical fertilizers to solve the problem of nutrient deficiency in soil has been associated with a number of environmental problems.Objective: The aim of this study was to perform an isolation and screening of native phosphofungi from mine soil. To evaluate the effect of phosphofungi on seed germination and seedling vigour index of Ground nut and Green gram.Materials and Methods: The phosphofungi were screened using Pikovskaya’s agar medium with tricalcium phosphate (Ca3 (PO4)2) as the phosphorus source. The soluble phosphorus, Phosphate solubilizing activity, titrable acidity, pH and fungal biomass were determined. Mineral phosphate solubilizing (MPS) activities of fungal isolates were tested in tricalcium phosphate medium.Results: Five promising phosphate solubilizing fungal species were screened. Aspergillus niger (13±1.0 mm) and Aspergillus flavus (8±0.6mm) showed the more phosphate solubilising index than compared to other fungal isolates. Analyzing the possible phosphorus released, from 3rd, 6th, 9th, and 12th day of incubation. Antagonistic effect of phosphofungi Aspergillus flavus (65±11 mm) and Aspergillus niger (60±13 mm) showed more effective against Colletotrichum sp. than compared to other fungal isolates. Aspergillus flavus (745±20µg/ml) and Penicillium spinulosum (600±20 µg/ml) showed maximum phosphate solubilizing activity on 3rd and 12th day of incubation. Fusarium redolens showed the more effectiveness on seed germination and seedling vigour than compared to other fungal isolates. Aspergillus flavus showed positive result for the production of Indole 3 acetic acid and remain four fungal strains are negative result.Conclusion: Biofertilizers from native phosphofungi could be used alongside reduced levels of inorganic fertilizers to enhance soil available Phosphorous.

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