Multivariate Analysis using D2 and Principal Component Analysis in Mung bean [Vigna radiata (L.) Wilczek] for Study of Genetic Diversity

2021 ◽  
Author(s):  
R.A. Jadhav ◽  
S.P. Mehtre ◽  
D.K. Patil ◽  
V.K. Gite
Keyword(s):  
Genetic Diversity ◽  
Mung Bean ◽  
Agricultural Research ◽  
Principal Component ◽  
Research Station ◽  
Mean Values ◽  
Breeding Lines ◽  
Cluster Distance ◽  
Improvement Programme ◽  
Bean Yield

Background: Seventy breeding lines of mung bean were evaluated for 20 different characters and mean values were worked for genetic diversity by Mahalanobis D2 statistic. Methods: The experiments included 70 mung bean breeding lines which were collected from Plant Breeding Unit, Agricultural Research Station, Badnapur. They were grown during Kharif 2016 at experimental research farm Badnapur of Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani. The data were recorded for 20 different characters on 10 randomly selected plants. The statistical analysis were done by Mahalanobis D2 statistics.Result: The results of D2 analysis indicated the presence of considerable genetic divergence among these breeding lines. In the present study, inter-cluster distances were worked out considering 20 characters and these distances ranged from 240.96 (between cluster V and cluster VII) to 1080.72 (between cluster V and cluster VIII). The inter-specific derivatives were grouped into eight clusters. The maximum inter-cluster distance was between cluster V and cluster VIII. The maximum inter-cluster distance was between cluster V and cluster VIII (1080.72), followed by cluster II and cluster III (932.25), cluster IV and cluster VIII (910.11), cluster VII and cluster VIII (738.30), cluster I and cluster VIII (732.61), cluster VI and cluster V (660.49) and cluster II and cluster VI (494.93). This suggested that there is wide genetic diversity between these clusters. Based on these studies, crosses can be made between breeding lines of these clusters to obtain desirable results either in transgressive breeding or in heterosis breeding. Cluster VIII and cluster IV showed high mean values for most of the yield contributing traits like100-seed weight, shelling %, harvest index, pod length, primary branches per plant, days to 50% flowering, days to maturity, leaf width and days to shattering. So the lines from cluster IV and cluster VIII can be used for mung bean yield improvement programme.

scholarly journals Genetic divergence analysis in papaya (Carica papaya L.) Genotypes

2016 ◽  
Vol 41 (4) ◽  
pp. 647-656
Author(s):  
N Ara ◽  
M Moniruzzaman ◽  
Ferdousi Begum ◽  
M Moniruzzaman ◽  
R Khatoon
Keyword(s):  
Genetic Diversity ◽  
Plant Height ◽  
Genetic Divergence ◽  
Carica Papaya ◽  
Agricultural Research ◽  
Research Station ◽  
Plant Weight ◽  
Mean Values ◽  
Fruit Length ◽  
Cluster Distance

The experiment on papaya (Carica papaya L.) consisting of fourteen genotypes from diversd gene pool was conducted at the Regional Agricultural Research Station, Ishurdi, Pabna during April 2013 to May 2014 to study the nature and magnitude of genetic divergence and eventually identification of suitable genotypes for use in breeding program. Multivariate analysis was subjected to assess the genetic diversity and Mahalanobis’ generalized distance (D2) was used to assess the divergence present among the genotypes. The fourteen genotypes were grouped into four clusters. The cluster IV had the maximum genotypes (5) followed by cluster I having 4 genotypes and cluster II having 3 genotypes. Cluster III had the minimum genotypes (2). The inter-cluster distances were greater than intra-cluster distances in all cases, suggesting wider genetic diversity among the genotypes of different groups. The highest intracluster distance was observed in cluster III and the lowest in cluster II. The maximum inter-cluster distance was estimated between clusters I and IV (11.3212), moderate distance between clusters II and IV (9.961) and clusters III and IV (7.568), and that of the lowest between clusters I and III. Cluster III recorded the highest mean values for fruit length, plant height at last harvest, number of fruits/plant, weight of fruits/plant and fruit yield, while cluster IV exhibited the maximum mean values for pulp thickness, plant height at 1st harvest and the second highest mean values for fruit length, fruit breadth and TSS. Therefore, more emphasis should be given on cluster III for selecting genotypes as parents for crossing with the genotypes of cluster IV which may produce new recombinants with desired traits.Bangladesh J. Agril. Res. 41(4): 647-656, December 2016

scholarly journals Genetic divergence of indigenous pummelo genotypes

2016 ◽  
Vol 3 (1) ◽  
pp. 37-43
Author(s):  
Md Sarowar Alam ◽  
Md Sultan Mia ◽  
Md Salim ◽  
Jubair Al Rashid ◽  
Md Saidur Rahman
Keyword(s):  
Genetic Divergence ◽  
Agricultural Research ◽  
Principal Component ◽  
Fruit Weight ◽  
Research Station ◽  
Mean Values ◽  
Cluster Distance ◽  
Minimum Number ◽  
Total Divergence ◽  
And Performance

The genetic divergence was studied in33pummelogenotypes using D2 statistics and principal component analysis at Regional Agricultural Research Station, BARI, Akbarpur, Moulvibazar during 2012 to 2014. The genotypes were grouped into 5 clusters and the maximum number of genotypes was included in cluster IV and V and the minimum number in cluster I. The inter cluster distance in all of the cases were higher than the intra cluster distance indicating wider genetic diversity among the accessions of different groups. The highest inter-cluster distance was observed between cluster I and II followed by cluster II and V and the lowest between III and IV. The highest intra- cluster distance was observed for the cluster II and the lowest for the cluster III. For cluster II, the highest mean values for plant height (6.13m), individual fruit weight (1141.67g), fruit length (13.03 cm) and breadth (13.15 cm), number of segments per fruit (14.41), number and weight of seeds per fruit (123.67 and 50.41g), yield per plant (50.94 kg) were observed. The first axis largely accounted for the variation among the pummelo accessions (26.16%) followed by second axis (18.75%). The first 8 axes accounted 90.56 % of the total variation. The characters individual fruit weight (g) and weight of seeds per fruit (g) showing positive value in both the vectors contributed maximum towards divergence. Considering magnitude of genetic distance, contribution of different traits toward the total divergence, magnitude of cluster means for different traits and performance the genotypes of cluster I, II, and IV may be considered as parents for future hybridization program.Res. Agric., Livest. Fish.3(1): 37-43, April 2016

↵Genetic Diversity and Principal Component Analysis in Mungbean [Vigna radiata (L.) Wilczek] under Rainfed Condition

2021 ◽  
Author(s):  
Deepak Gupta ◽  
Suresh Muralia ◽  
N.K. Gupta ◽  
Sunita Gupta ◽  
M.L. Jakhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Seed Yield ◽  
Agricultural Research ◽  
Principal Component ◽  
Component Analysis ◽  
Grain Legume ◽  
Research Station ◽  
Rainfed Condition

Background: Mungbean is a short duration grain legume widely grown in south and Southeast Asia. The extent of variability through Principal Component Analysis (PCA) and cluster analysis in promising mungbean genotypes should be known for possible yield improvement. A study was undertaken to work out the extent of variability among twenty four mungbean genotypes through cluster analysis and Principal Component Analysis (PCA). Methods: The experiment was laid out in a randomized block design with three replications during kharif 2018 and 2019 at the experimental field of Agricultural Research Station, Navgaon (Alwar) under rainfed condition. Result: Principal component analysis revealed that the first three main PCAs amounted 78.80% of the total variation among genotypes for different traits. Out of total principal components, PC1 accounts for maximum variability in the data with respect to succeeding components. Number of branches per plant (28.62%), number of clusters per plant (23.55%) and seed yield (15.58%) showed maximum per cent contribution towards total genetic divergence on pooled basis. Cluster analysis showed that genotypes fall into seven different clusters and their inter and intra cluster distance showed genetic diversity between different genotypes. The maximum number of genotypes i.e., 8 was found in cluster II followed by cluster III comprising of 6 genotypes. Genotypes RMG-1138 and IPM-02-03 representing the mono genotypic cluster signifies that it can be the most diverse variety and it would be the appropriate genotype for hybridization with ones present in other clusters to tailor the agriculturally important traits and ultimately to boost the seed yield in mungbean under rainfed conditions.

scholarly journals Genetic Divergence in Pointed Gourd

1970 ◽  
pp. 87-92 ◽  
Author(s):  
MY Kabir ◽  
ASMMR Khan ◽  
MS Hassain
Keyword(s):  
Genetic Diversity ◽  
Genetic Divergence ◽  
Agricultural Research ◽  
Growing Season ◽  
Mean Value ◽  
Research Station ◽  
Cluster Distance ◽  
Late Maturity ◽  
Pointed Gourd ◽  
Clustering Pattern

The experiment was conducted at the Regional Agricultural Research Station, Ishurdi, Pabna during the growing season 2005-2006 to estimate the genetic diversity among 24 genotypes of pointed gourd by using Mahalanobis D² statistics for nine characters. The genotypes were grouped in to five clusters. The cluster I and III consisted of highest number of genotypes and it was six. The cluster IV contained the lowest number of genotypes and it was three. The clustering pattern of the genotypes under this study revealed that the genotypes collected from the same location were grouped into different clusters. The genotypes of Kushtia were distributed in different clusters. The inter cluster distance were larger than the intra cluster distance suggesting wider genetic diversity among the genotypes of different groups. The highest intra cluster distance was computed for cluster IV (35.80) and the minimum intra cluster distance was found in cluster III (18.37). The clusters IV and II were more diverse as indicated by maximum inter cluster distances between them (41.56) and the minimum inter cluster divergence was observed between cluster III and II (6.84). Cluster II had the highest cluster mean value for number of fruits per plant (391), weight of fruit per plant (11.72kg) and yield (35.28t/ha). Genotypes of the cluster V had late maturity. Key words: Genotype; genetic diversity; pointed gourd. DOI: 10.3329/jard.v7i1.4426 J Agric Rural Dev 7(1&2), 87-92, June 2009

scholarly journals Genetic Diversity In Exotic Maize (Zea mays L.) Hybrids

2013 ◽  
Vol 38 (2) ◽  
pp. 335-341 ◽  
Author(s):  
MA Zaman ◽  
MA Islam
Keyword(s):  
Plant Height ◽  
Genetic Divergence ◽  
Agricultural Research ◽  
Research Station ◽  
Early Maturity ◽  
Mean Values ◽  
Maize Hybrids ◽  
Lattice Design ◽  
Cluster Distance ◽  
The Mean

An experiment in alpha lattice design with three replication including 39 exotic maize hybrids was conducted at the Research farm of Regional Agricultural Research Station, BARI, Ishuardi, Pabna during Rabi season 2010-11 for analysis the genetic divergence in exotic maize hybrids. The genotypes were grouped in to seven clusters. Cluster VI comprised the maximum genotypes (13) indicating overall genetic similarity among them. The minimum genotype (1) was contained in the cluster III and V. The highest inter-cluster distance was observed between cluster V and III followed by cluster I and III and cluster III and VII suggesting wide diversity between them and the genotypes in these cluster could be used in hybridization program for obtaining a wide spectrum of variation among the segregates. The highest intra-cluster distance was observed in cluster VII and the cluster III and V were contained only one genotype and hence, their intra cluster distance was zero. The mean values of cluster IV recorded the highest yield per hectare (11.60 ton/ha) with medium plant height, days to maturity, days to 50% tasseling, silking and shelling percentage. Selection on the basis of plant aspect and ear aspect the genotypes of cluster III ranked first but plant height was high with medium seed size, medium yield, medium shelling percentage and also in late in case of maturity. The mean values of cluster V shown overall medium in case of yield and all yield contributing characters. Qualitative characters contribute maximum towards genetic divergence. Therefore, the genotypes from cluster III, V and VI could be utilized as source materials for getting desirable new recombinants with early maturity and higher yield. Bangladesh J. Agril. Res. 38(2): 335-341, June 2013 DOI: http://dx.doi.org/10.3329/bjar.v38i2.15894

scholarly journals Study on genetic diversity of pointed gourd using morphological characters

1970 ◽  
Vol 33 (4) ◽  
pp. 607-616 ◽  
Author(s):  
ASMR Khan ◽  
MG Rabbani ◽  
MA Siddique ◽  
MI Hossain
Keyword(s):  
Genetic Diversity ◽  
Agricultural Research ◽  
Morphological Characters ◽  
Mean Value ◽  
Fruit Weight ◽  
Research Station ◽  
Cluster Distance ◽  
Total Divergence ◽  
Pointed Gourd ◽  
Five Axes

The genetic diversity among 64 pointed gourd genotypes were assessed through multivariate analysis from an experiment conducted in Regional Agricultural Research Station, lshurdi, Pabna during the growing season 2002-2003. The genotypes were grouped into twelve clusters. The cluster V consisted of highest number of genotypes and it was nine, the cluster VI and cluster VIII contained the lowest number of genotypes and it was two in each. The clustering pattern of the genotypes under this study revealed that the genotypes collected from the same location were grouped into different clusters. The genotypes of Jessore were distributed in different clusters. The highest inter genotype distance as 366.3 observed between the genotypes P0022 and P0007 and the lowest 2.6 as observed between the genotypes P0043 and P0044. Cluster V had the highest cluster mean value for internode length, fruit weight per plant and yield the highest inter-cluster distance was noticed between cluster III and II (45.71) and the lowest between cluster VII and VI (3.33). ‘The highest intra cluster distance was computed for cluster III and that was lowest for the cluster II. The first five axes accounted for 77.65% of the total variation among the 13 characters describing 64 pointed gourd genotypes. Fruit weight, seeds per fruit and fruit weight per plant contributed maximum to the total divergence. The results obtained by D2 analysis were confirmed by canonical analysis. Key Words: Diversity pointed gourd, morphological characters. doi: 10.3329/bjar.v33i4.2304 Bangladesh J. Agril. Res. 33(4) : 607-616, December 2008

scholarly journals Variability and Genetic Diversity of Bottle Gourd [Lagenaria siceraria (mol.) Stand.] in Bangladesh

2020 ◽  
Vol 11 (2) ◽  
pp. 126
Author(s):  
A. K. M. Quamruzzaman ◽  
F. Islam ◽  
M. M. R. Salim ◽  
L. Akhter ◽  
M. M. Rahman ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Agricultural Research ◽  
Principal Component ◽  
Fruit Shape ◽  
Fruit Weight ◽  
Fruit Yield ◽  
Bottle Gourd ◽  
Agricultural Research Institute ◽  
Quantitative Characters ◽  
Cluster Distance

The presented study was conducted at the experimental field of Olericulture Division, Horticulture Research Center, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh during the year 2018-19 to assess the extent of genetic diversity among 25 bottle gourd germplasms. Data on: days to first harvest, fruit number per plant, average fruit weight, fruit yield (kg/plant), fruit length, fruit diameter, fruit yield (t/ha), fruit shape and fruit color were recorded.  Plot means for quantitative characters were used for the statistical analysis, while genetic diversity, principal component analysis (PCA), principal Coordinate analysis (PCO), cluster analysis, cluster means and two-dimensional scattered plotting diagram were studied. The germplasms were constellated into five distinct groups with the range of 3 germplasms in the cluster III to 6 germplasms in the clusters II and IV. The inter-cluster distance in all cases was larger than is the intra-cluster distance. The highest intra-cluster value (2.65) was found in the cluster IV. Maximal inter-cluster distance (12.24) was observed between germplasm of cluster I and V, followed by the clusters I and III (12.05) and minimal was found between germplasms of cluster I and IV (3.46). In case of the cluster means values and yield contributing performance cluster I, cluster IV and cluster III performed well. Considering the group distance and inter-genotypic crosses between the clusters and cluster means, inbreeds belong to clusters: I, IV and III, to that will be given higher priority for crossing in future bottle gourd hybridization program.

scholarly journals Genetic diversity analysis of parental lines for hybrid development in rice (Oryza sativa L.)

2013 ◽  
Vol 37 (4) ◽  
pp. 617-624 ◽  
Author(s):  
MJ Hasan ◽  
Umma Kulsum ◽  
MMH Rahman ◽  
MMH Chowdhury ◽  
AZMKA Chowdhury
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa L ◽  
Principal Component ◽  
Mean Value ◽  
Cluster Distance ◽  
Parental Lines ◽  
Almost All ◽  
Yield Maximization ◽  
Hybrid Development ◽  
Panicle Weight

Genetic divergence of 40 parental lines comprising 30 restorer and 10 maintainer lines were studied through Mohalanobis's D2 and principal component analysis for eleven characters. Genotypes were grouped into five different clusters. Cluster V comprised maximum number of genotypes (thirteen) followed by cluster I and II. The inter-cluster distance was maximum between clusters I and V (13.495) indicating wide genetic diversity between these two clusters followed by the distance between cluster I and 11 (9.489), cluster IV, and cluster V (8.969) and cluster I and cluster III (8.039). The minimum inter-cluster distance was observed between cluster II and cluster III (3.034) followed by cluster 111 and cluster IV (3.834) and cluster II and cluster V (4.945) indicating that the genotypes of these clusters were genetically close. The intra cluster distance in the entire five clusters was more or less low which indicated that the genotypes within the same cluster were closely related. Among the characters panicle weight contributed most for divergence in the studied parental lines. Difference in cluster means existed for almost all the characters studied. Highest mean value for number of effective tillers (7.8), days to 50% flowering (95.5), panicles/m2 (192.6), panicle weight (2.9), spikelet fertility (84.8), number of grains/panicle (177.8), days to maturity (123.6), and grain yield/plot (1065.5) were observed in cluster I indicated the parental lines fallen in this cluster having the genetic potentiality to contribute better for yield maximization of hybrid rice. DOI: http://dx.doi.org/10.3329/bjar.v37i4.14386 Bangladesh J. Agril. Res. 37(4): 617-624, December 2012

scholarly journals Genetic diversity and phylogenetic behavior of some minor legumes

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
James Oludare Agbolade ◽  
Ronke Justina Komolafe
Keyword(s):  
Genetic Diversity ◽  
Principal Component ◽  
Calyx Lobe ◽  
Descriptive Statistics ◽  
International Institute ◽  
Mean Values ◽  
Phylogenetic Relatedness ◽  
Petal Length ◽  
Morphological Differences ◽  
Range Test

Twenty-four accessions of twelve species minor legumes collected from the germplasm unit of the International Institute of Tropical Agriculture Ibadan, Nigeria were evaluated for their genetic diversities and phylogenetic relatedness. The accessions were planted into plots of 5 ridges of 5 meters long, spaced 1 meter apart and replicated three times at the Federal University Oye-Ekiti Teaching and Research Farm. The diversity and the relative phylogeny of the accessions were assessed through their floral morphological differences and the mean values between two accessions were evaluated by descriptive statistics. Principal component analysis was employed to identify the most discriminatory floral morphological traits and the similarities among the 24 accessions were assessed by cluster analysis (CA). Descriptive statistics through Duncan multiple range test adopted revealed genetic diversity and phylogenetic relatedness among the accessions. The first two principal component axes explained 64.66% of the total floral morphological variation. Standard petal length, calyx lobe length and stipule length contributed most of the variations in the legume accession. CA grouped the 24 accessions into six clusters. The study revealed intra-specific similarities and inter-specific floral morphological differences among the studied accessions.

scholarly journals Phenotypic Screening of F4 Breeding Lines against Bacterial Blight Disease in Rice

2021 ◽  
pp. 299-304
Author(s):  
K. Alekya ◽  
B. Laxmi Prasanna ◽  
M. Balram ◽  
N. Balram ◽  
P. Gonya Nayak ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Agricultural Research ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Research Station ◽  
Rice Grain ◽  
Disease Score ◽  
Breeding Lines ◽  
Biotic Stresses ◽  
Resistant Reaction

Biotic stresses are major threat to rice production. Among biotic stresses, bacterial leaf blight is one of the major diseases affecting rice grain production in rice growing areas. Present investigation was conducted to evaluate phenotypic effect of 50 breeding lines from a cross (Pranahitha//ISM/MTU1010) in glass house at Regional Agricultural Research Station, Jagtial by inoculating Bacterial leaf blight culture (DX-020) by leaf clipping method. Out of these 50 F4 lines evaluated, twenty nine breeding lines showed resistant reaction with disease score of 1. Eleven lines showed moderately resistant reaction with disease score 3. Twenty nine breeding lines that were resistant with disease score 1, had excellent grain yield. Hence, these lines can be advanced to further generations. Thus the present study has demonstrated that phenotypic selection is successful in the glass house and these breeding lines with higher yield levels are expected to perform better in the field trials and further in the farmers fields with the good level of bacterial blight resistance.

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