scholarly journals Priming seed mitigates the effects of saline stress in soybean seedlings

2019 ◽  
Author(s):  
Z. Miladinov ◽  
I. Maksimovic ◽  
V. Djordjevic ◽  
S. Baleseviæ-Tubic ◽  
P. Canak ◽  
...  
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seed Quality ◽  
Germination Rate ◽  
Pearson Correlation ◽  
Soybean Seed ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Saline Stress ◽  
Soybean Seedlings

Seed germination and seedling growth are the most sensitive plant stages towards salinity. Various techniques can improve emergence and stand formation under salt conditions. Seeds priming is one of the most frequently used technique. Seed priming could develop different defence mechanisms of seeds against salinity stress. This experiment aims to examine the seed priming into potassium nitrate - KNO3 (1%), ascorbic acid – ASA (100mgl-1), and potassium chloride - KCl (1%) solutions, that may reduce harmful effects of salinity stress. Salinity was imposed by treatment of seeds with different concentrations of NaCl (0 (control), 50, 100, 200m MNaCl). All data were analysed statistically by three-way ANOVA. Individual testing of probability was carried out using Tukey’s method p £ 0.05 and p £ 0.01. Correlation dependence was determined using the Pearson correlation coefficient. Test results demonstrated positive effect of seed priming. This was showed by improved soybean seed quality and increased seed germination rate. The intensity of lipid peroxidation, free proline concentration and Na+ was decreased, while K+ in seedlings increased due to priming treatment. Priming of seeds into ASA solution resulted in increased vitamin C concentration, while priming into KNO3 and ÊCl solutions reduced the content of vitamin Ñ in soybean seedlings.

scholarly journals The Use of Electrostatic Field to Improve Soybean Seed Germination in Organic Production

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1473
Author(s):  
Zlatica Mamlic ◽  
Ivana Maksimovic ◽  
Petar Canak ◽  
Goran Mamlic ◽  
Vojin Djukic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Electrostatic Field ◽  
Seed Quality ◽  
18Th Century ◽  
Soybean Seed ◽  
Organic Production ◽  
Initial Growth ◽  
Physical Treatment ◽  
Soybean Seedlings ◽  
Dark Green

Soybean production in the system of organic agriculture is not very demanding, and this has been well documented both through experimental results and commercial production. However, one of the biggest problems in organic production is the lack of adequate pre-sowing treatments. Therefore, the aim of this study was to examine the effect of the electrostatic field. This is a physical treatment that was first used for seed treatment in the 18th century but has mostly been neglected since then. Seeds of five soybean genotypes with differently colored seed coats (yellow, green, dark green, brown, and black) were included in this study. The seeds were exposed to different values of direct current (DC) with the following voltages: 0 V (control), 3 V, 6 V, and 9 V, to which the seeds were exposed for 0 min (control), 1 min, and 3 min. After exposing the seeds to the electric field, the physiological properties of seeds and seedlings at the first stage of growth were evaluated. The results show that the effect of the electrostatic field on seed quality depends on the genotype, voltage, and exposure time. The application of DC can be a suitable method for improving seed germination and the initial growth of soybean seedlings. In addition, the results indicate that it is necessary to adjust the DC treatment (voltage and duration of exposure of seeds) to particular genotypes since inadequate treatments may reduce the quality of seeds.

scholarly journals Effect of low frequency magnetic field (LFMF) on seed quality of radish (Raphanus sativus L.) seeds

2020 ◽  
Vol 48 (3) ◽  
pp. 1458-1464
Author(s):  
Xian-Zong XIA ◽  
Gregorio PADULA ◽  
Leszek KUBISZ ◽  
Roman HOŁUBOWICZ
Keyword(s):  
Magnetic Field ◽  
Seed Germination ◽  
Raphanus Sativus ◽  
Seed Quality ◽  
Germination Rate ◽  
Low Frequency ◽  
Seed Priming ◽  
Raphanus Sativus L ◽  
Frequency Magnetic Field

In recent years, the application of magnetism in agriculture has been paid more and more attention to, especially in the field of its treatment on the seed germination and physiological indexes of seedlings grown out of them. In this experiment, the radish (Raphanus sativus L.) seeds of two cultivars ‘Carmen’ and ‘Szkarłatna z Białym Końcem’ were treated by 20 mT low frequency magnetic field (LFMF) for 10, 30 and 60 minutes, respectively. The MF was generated from a Viofor JPS Delux - a patented device adopted from the routine medical magnetic therapy. By measuring their seed germination rate (energy), seedling length and fresh weight, it was proved that LFMF improved the seed quality of both radish cultivars and the best results were received for the longest exposing time. The received that way results were similar as reported for priming of radish seeds. The developed treatment has a great potential in replacing traditional seed priming methods. However, for its commercial use, for selected crops and cultivars, further research is still needed.

scholarly journals Potensi Ekstrak Kulit Buah Naga untuk Mitigasi Cekaman Salinitas pada Perkecambahan Benih Kedelai

2020 ◽  
Vol 4 (2) ◽  
pp. 106
Author(s):  
Maman Suryaman ◽  
Ida Hodiyah ◽  
Neng Inten
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Germination Rate ◽  
Block Design ◽  
Soybean Seed ◽  
Dry Weight ◽  
Germination Percentage ◽  
Randomized Block Design ◽  
Dragon Fruit ◽  
Peel Extract

<p>Salinity stress has negative effect on seed germination; therefore, it is necessary to find technology to mitigate it. The research was conducted to study the potency of peel extract of dragon fruit to mitigate salinity stress on soybean seed germination. This research used a randomized block design with factorial patterns and three replications. The first factor was the level of salinity of NaCl (C), consisted of 3 levels (c<sub>0</sub> = 0%, c<sub>1</sub>=0.5%, c<sub>2</sub>=1%), The second factor was peel extract of dragon fruit (I), consisted of 3 levels (i<sub>0</sub> = 0% (control), i<sub>1 </sub>= 1%, and i<sub>2</sub>=2%). The parameters analyzed were germination percentage, germination rate, root length, epicotyl length, electric conductivity, and dry weight of sprout. The data were analyzed with ANOVA and Duncan’s multiple range test at α = 5 %. The result showed no interaction between salinity stress and peel extract of dragon fruit on soybean seed germination. Salinity stress significantly decreased sprout vigor with the highest decrease was shown at 1% salinity; on contrarily peel extract of dragon fruit could maintain sprout vigor. Peel extract of dragon fruit 2% was the potential to mitigate salinity stress.</p>

scholarly journals Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh
Keyword(s):  
Seed Germination ◽  
Climate Variability ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Seedling Development ◽  
Hot Water ◽  
Crop Failure ◽  
Simple Method ◽  
Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

scholarly journals Muskmelon seed germination and seedling development in response to seed priming

2003 ◽  
Vol 60 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Germination Rate ◽  
Fungal Growth ◽  
Seed Priming ◽  
Seedling Development ◽  
Factors Affecting ◽  
Total Seed ◽  
Cucumis Melo L ◽  
Osmotic Potentials

Important factors affecting seed priming have not been extensively reported in muskmelon (Cucumis melo L.) studies. The optimization of the seed priming technique becomes very important at the commercial scale. Little information has been reported on seedling development of muskmelon subsequent to seed priming. Seeds of muskmelon were primed in darkness at 25°C in different solutions and three osmotic potentials. Seeds were also primed with and without aeration during different periods. In relation to osmotic solutions, an osmotic potential around -1.30 MPa is most adequate for muskmelon priming. Salt solutions gave better germination rate but were deleterious for seed germination, especially at higher osmotic potentials. Aeration of the soaking salt solution gave faster germination at 17°C, and because of the early germination, these treatments probably presented a better seedling development. Deleterious effect on total seed germination was observed for long soaking periods with aeration. Fungal growth increased on seeds primed in aerated solutions. Seeds from priming treatments had a better germination rate and seedling development under 17 and 25°C.

scholarly journals High gamma irradiation doses and long storage times reduce soybean seed quality

2016 ◽  
Vol 37 (3) ◽  
pp. 1219
Author(s):  
Danúbia Aparecida Costa Nobre ◽  
Carlos Sigueyuki Sediyama ◽  
Valter Arthur ◽  
Newton Deniz Piovesan ◽  
Alisson Santos Lopes da Silva
Keyword(s):  
Gamma Irradiation ◽  
Seed Quality ◽  
Germination Rate ◽  
Soybean Seed ◽  
Response Surfaces ◽  
Seed Vigor ◽  
Production Cycle ◽  
High Gamma ◽  
Storage Times ◽  
Long Storage

High quality seeds are required for soybean production. This study evaluated the effect of gamma irradiation and storage time on seed quality in soybean lines VX04-6828 and VX04-5692. Seeds were gamma irradiated (60Co) with 0, 50, 150, and 250 Gy. After the first seed production cycle (M1), the harvested seeds were stored in the laboratory for 0, 2, 4, and 6 months. Moisture content, seed quality (germination rate, dead seeds, and normal and abnormal seedlings), and seed vigor (first germination count, germination index, and seedling length) were determined. Data were submitted to analysis of variance for each soybean line using a 4 x 4 factorial design (four storage times x four gamma irradiation doses). Response surfaces were constructed based on the F test significance (p ? 0.05). VX04-5692 seeds were more sensitive to gamma radiation than were VX04-6828 seeds. Soybean seed quality was highest in M2 seeds derived from seeds irradiated with less than 100 Gy and stored for up to two months. High gamma irradiation doses and long storage times reduced soybean seed quality.

scholarly journals The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Principal Component ◽  
Dry Weight ◽  
Seed Priming ◽  
Nacl Stress ◽  
Future Research ◽  
Fresh Weight ◽  
Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

scholarly journals Cryopreservation of primed tobacco seeds

2018 ◽  
Vol 40 (4) ◽  
pp. 415-421
Author(s):  
Camila Aparecida Lopes ◽  
Maria Laene Moreira de Carvalho ◽  
Ana Cristina de Souza ◽  
João Almir Oliveira ◽  
Dayliane Bernardes de Andrade
Keyword(s):  
Seed Germination ◽  
Ambient Temperature ◽  
Silica Gel ◽  
Saline Solution ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Water Bath ◽  
Tobacco Seeds ◽  
Physiological Quality

Abstract: Priming is a commercial technique used to increase the speed and uniformity of seed germination. However, the physiological quality of primed seeds is usually negatively affected during storage. Tobacco seeds of the cultivar BAT 2101 were used to investigate whether primed seeds could be cryopreserved. The most suitable substances, type of drying, and reheating during priming and cryopreservation of tobacco seeds were studied. Seed priming was performed with water, spermidine, and potassium nitrate, and drying was carried out with silica gel and a saturated saline solution. Seeds were cryopreserved in liquid nitrogen for 24 h and reheated in a water bath for two and five minutes and at ambient temperature. Tobacco seeds primed with spermidine and water can be cryopreserved without loss of physiological quality when quickly dried on silica gel and reheated in a water bath for two minutes.

scholarly journals Physiological quality of osmoprimed Eremanthus erythropappus (D.C.) MacLeish seeds

2020 ◽  
Vol 13 (5) ◽  
pp. 18
Author(s):  
A. M. S. Oliveira ◽  
A. A. Silva ◽  
M. C. Vasconcelos ◽  
J. A. A. Granja ◽  
J. M. R. Faria ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Germination Rate ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
The Other ◽  
Other Substances ◽  
Physiological Quality ◽  
Glycol Solution ◽  
Polyethylene Glycol 6000

Physiological conditioning is a technique that consists in controlling the speed of water uptake by seeds, with osmotic solutions, aiming to improve their quality, reducing time and increasing germination rate. Eremanthus erythropappus (D.C.) MacLeish seeds were subjected to priming with polyethylene glycol 6000 (PEG), potassium nitrate (KNO3) and the combination of the two at the concentrations of 0.4; -0.8; -1.0; -1.2 and -1.4 MPa for four, six and eight days. Germination, germination speed index (GSI), emergence, emergence speed index (ESI) and expression of superoxide dismutase (SOD), catalase (CAT) and peroxidase (PO) enzymes were assessed. E. erythropappus seed priming in polyethylene glycol solution with potentials between -0.8 MPa and -1.4 MPa is not satisfactory because it adversely affects germination and reduces the activity of peroxidase and catalase enzymes. The other substances were not significant so there is a need for further research with other substances and / or concentrations

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