scholarly journals Influence of Salt Stress on Seed Germination and Agro-morphological Traits in Chickpea (Cicer arietinum L.)

Author(s):  
Munqez J.Y. Shtaya ◽  
Heba Al-Fares ◽  
Tawfiq Qubbaj ◽  
Hassan Abu-Qaoud ◽  
Faisal Shraim

Background: The experiment was conducted at the Faculty of Agriculture and Veterinary Medicine, An-Najah National University, Palestine under growth chamber and greenhouse conditions. Methods: To study the effect of NaCl on germination, fifty seeds in each treatment for each cultivar were germinated on a filter paper in petri dishes moistened with the different NaCl concentration levels (0.0, 50 and 100 mM ). To study the effect of NaCl on plant growth and productivity, 2 seeds per variety were grown in 10 liter pots filled with peat moss-sand. Pots were irrigated with the different NaCl levels. Result: The tested Chickpea cultivars showed different tolerance levels in response to NaCl stress levels. NaCl reduced final germination percentage (FGP) and germination index (GI). The highest plant height was obtained by Einalbeda (38.63 cm) in the control group, while the lowest one was found in Arij (28.25 cm) under 100 mM NaCl. NaCl did not affect root/shoot ratio in all genotypes. Reduction in fresh weight ranged between 25.6 and 74.2% under 50 and 100 mM NaCl.

2014 ◽  
Vol 67 (1) ◽  
pp. 115-123 ◽  
Author(s):  
H. Saeb ◽  
M. Khayyat ◽  
A. Zarezadeh ◽  
F. Moradinezhad ◽  
A. Samadzadeh ◽  
...  

Abstract The present research was conducted to evaluate the effects of salinized water with NaCl on seed germination of Periwinkle and Corn Poppy. Treatments were: A) H2O- distilled water (control); B) sodium chloride solutions (5, 10, 20, 40 and 80 mM). Application of the highest NaCl concentration (80mM) significantly reduced germination percentage and mean germination time of both species, although increased the day of 50% germination. Increment of salinity concentration was led to the reduction of radicle length in Periwinkle. The seedling fresh weight and water content and radicle length of Corn Poppy were decreased in both levels of 20 and 80 mM, and seedling dry weight was unaffected by treatments. It is concluded that both species are tolerant to NaCl salinity up to 80 mM during germination stage.


2019 ◽  
Vol 47 (4) ◽  
pp. 1285-1292
Author(s):  
Yuneisy Milagro AGÜERO-FERNÁNDEZ ◽  
Luis Guillermo HERNÁNDEZ-MONTIEL ◽  
Bernardo MURILLO-AMADOR ◽  
José Manuel MAZÓN-SUÁSTEGUI ◽  
Carlos Michel OJEDA-SILVERA ◽  
...  

Salinity stress is one of the main problems limiting growth and development of cultivated species. The objective of this study was to assess NaCl-stress basil plants (Ocimum basilicum L.) cv. ‘Nufar’ and to determine whether the mitigating effect of an arbuscular mycorrhizal fungus strain (AMF). A completely randomized factorial design was used considering three NaCl concentration (0, 50 and 100 mM) as factor 1 and presence or absence of AMF (0 and 10 g of inoculum) as factor 2, with four replicates per treatment and four plants per repetition. The assessed response variables were, fresh and dry of aerial part and root, root length, leaf area, relative water content, water potential, plant height, number of spores and mycorrhizal colonization percentage after 20 and 50 days (T20, T50) of the experiment. The results showed greater values in all variables in the control group (0 mM NaCl) than in plants inoculated with R. fasciculatum with T20 and T50; although values decreased as NaCl concentration increased; the tendency to increase was maintained even in at 50 and 100 mM of NaCl with AMF with respect to 50 and 100 mM NaCl without AMF. The AMF colonization percentage decreased as NaCl concentration increased. Nonetheless, the development and growth response for all variables in the inoculated plants with AMF was greater vs non-inoculated, which suggests that basil plant inoculation with AMF has a positive effect in mitigating NaCl stress.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. A. Gaber ◽  
A. F. Abou-Hadid ◽  
Y. A. El- Gabry ◽  
M. H. M. Ebid

In Egyptian sugarcane breeding program, a pot experiment was carried out during 2019 season at Agricultural Research Station, Giza Governorate (latitude 26o 33? N and longitude 31o 12? E), Egypt, to evaluate twenty sugarcane clones, compared with the cultivated variety GT.54-9, under three irrigation water levels IWL (100, 80 and 60% of IWL). The traits FW of the shoot and root, root: shoot ratio, LAI, LAR, Chla, Chlb, Chla: Chlb ratio, carotenoids and proline were assessed. From this study clones 17 had height shoot fresh weight under water stress condition, as same as, clones 1, 18 and 19 had great behavior under water stress. In addition to most of sugarcane tested clones were not affected by increase the degree of water stress from 100 to 80% of IWL. The LAI, Chl.a and Chl.b traits showed the high correlation with shoot fresh weight, whereas, proline had strong relationships with root fresh weight under sugarcane drought stress.


Author(s):  
Ferit Özen ◽  
Gülsüm Yaldız ◽  
Mahmut Çamlıca ◽  
Halit Aşkın ◽  
Abdurrahman Başol

This research was carried out in the climate chamber in order to determine the effect of hydrosols obtained from sage grown with selenium application on germination of fenugreek and coriander seeds. Different concentrations of sage hydrosol (2.5, 5, 7.5 and 10 ml) and purified water used as control were applied to the fenugreek and coriander seeds. Germination rate, fresh weight, dry weight, root length, shoot length and root / shoot ratio of fenugreek and coriander seeds were applied. Variance analysis was performed according to the split plot design and the mean values were compared with LSD test. According to the results of the research; germination rate of fenugreek seeds 38.35-51.00%, fresh weight 0.04-0.12 g, dry weight 0.001-0.035 g, root length 0.71-2.02 cm, shoot length 1.27-3.20 cm, root/shoot ratio varied between 0.34-0.82, germination rate of coriander seeds 18.61-39.21%, fresh weight 0.0011-0.034 g, dry weight 0.004-0.030 g, root length 1.5-4.58 cm, shoot length 1.08-3.69 cm, root/shoot ratio 0.47-2.51 were found. The highest germination rate was obtained in 2.5 ml dose of sage hydrosol (51.00%) in fenugreek seeds and the highest germination rate was found in 7.5 ml dose of sage hydrosol (39.21%) in coriander seeds.


ISRN Botany ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Michael P. Fuller ◽  
Jalal H. Hamza ◽  
Hail Z. Rihan ◽  
Mohammad Al-Issawi

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at  MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.


Author(s):  
Nurgül Ergin ◽  
Mehmet Demir Kaya

This study was aimed to determine the responses of some sunflower hybrids to drought and low temperature stresses during germination and seedling development period. Sunflower hybrids of Sanbro MR, 63LE113, 64LL62 and Meteor were used in the study. Germination percentage, mean germination time, root and shoot length, root / shoot ratio, seedling fresh and dry weight of the sunflower hybrids were investigated at two temperatures (low 15°C and optimum 25°C) and drought stresses (distilled water, -1.5, -3.0, -4.5 and -6.0 bar) induced by polyethylene glycol 6000. The results of the research showed that there were significant differences among the sunflower hybrids in terms of the investigated characteristics. Increased drought stresses led to decreasing germination percentage, root and shoot length, seedling fresh weight, but caused a prolonged germination time and increased seedling dry weight. No significant change was observed in the germination and seedling development of the sunflower up to drought of -3,0 bar. The root / shoot ratio of sunflower hybrids increased at 15°C. The Sanbro MR had the fastest and highest germination along with the highest seedling fresh and dry weight at low temperature and higher drought stresses. It was concluded that sunflower hybrid Sanbro MR gave better performance under drought and low temperature conditions than the other hybrids.


2017 ◽  
Vol 28 (1) ◽  
pp. 18-25 ◽  
Author(s):  
R Ahmed ◽  
MHK Howlader ◽  
A Shila ◽  
MA Haque

An incubation experiment was conducted at the Department of Soil Science, Patuakhali Science and Technology University during Rabi season 2015 to investigate the effects of different levels of salinity on germination and early seedling growth of maize. There were ten treatments consisting of different concentrations of salinity viz. 0, 20, 40, 80, 120, 160, 200, 240, 280 and 320 mM NaCl. The germination experiment was conducted in petri dish lined with a layer of cotton consisting ten ml of each test solution. Germination percentage gradually decreased with the increase of concentration of salt. Up to 80 mM concentration was found safe for maize seed germination. Salinity caused delay in germination.  Highest seedling height was found at 40 mM NaCl concentration (21.51 cm) and root length (23.61 cm) was found in 20 mM NaCl concentration. In 320 mM NaCl concentration roots were abnormal, deformed and twisted. The 0 to 80 mM NaCl concentration gave statistically similar shoot fresh weight. Compared to control treatment 20 mM NaCl concentration gave 0.55 % higher fresh weight and at 80 mM NaCl concentration shoot fresh weight reduced by only 6.9 %. The 80 mM NaCl concentration was found acceptable for germination and early seedling growth of maize.Progressive Agriculture 28 (1): 18-25, 2017


2021 ◽  
Author(s):  
Shipeng Yan ◽  
Peifang Chong ◽  
Ming Zhao ◽  
Hongmei Liu

Abstract Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s salinity response have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was negatively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47, 177, 136 differentially expressed proteins (DEPs) were identified in low-salt vs. CK, high-salt vs. control, and high-salt vs. low-salt comparisons, respectively. A total of 72 DEPs were further screened from the groups, of which, 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 point) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated vs. CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis enhancing breeding, under salt stress conditions.


Author(s):  
Adinda Wuriandani ◽  
Sobir Sobir ◽  
Desta Wirnas ◽  
Agung Wahyu Susilo

Drought stress is a major constraint in cocoa production. The use of drought tolerant clone is the most efficient tool to overcome drought problem in cocoa production. North Carolina II (NCII) mating-design was used to identify parental and progeny performance in drought stress. The crossing consisted of three female parent clones namely KW 516, Sulawesi 3, and TSH 858, while male parent clones were ICCRI 09 and Scavina 6 then produced 6 combinations crosses. Genotypes used were 11 genotypes consisted of 5 parent clones and 6 cross genotypes. Drought treatment was applied to cocoa seedlings at 6 weeks after sowing with 25% and 100% available water content. The plants were maintained without water for five days to modulate the drought intensity. Variables observed were stem diameter, root length, root volume, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, and root/shoot ratio characters at 16 weeks after sowing. Based on the estimated general combining ability (GCA) value, TSH 858 was the best female parent, while Scavina 6 was the best male parent. Based on the estimated specific combining ability (SCA) value Sulawesi 3 x ICCRI 09 and TSH 858 x Scavina 6 were the best crosses. Based on the estimation of its genetic components, characters of drought tolerance stress were affected by additive genes. The dominant gene only affected the root fresh weight and root/shoot ratio. Based on SSI values, TSH 858 and Sulawesi 3 clones were drought-resistant clones, ICCRI 09 was moderate clone, and KW 516 and Scavina 6 were susceptible. Some of the findings were in contrast with earlier study.


Author(s):  
A.M. Khorshid ◽  
F.A. Moghadam ◽  
I. Bernousi ◽  
S. Khayamim ◽  
A. Rajabi

This study was carried out in the Agricultural Research Center of West Azerbaijan, Iran in 2016. In this research, variations in different physiological and yield traits measurement of total dry weight, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, relative water content, relative water loss, root length, leaf area, root/shoot ratio, specific leaf weigh, sodium content, potassium content and proline were investigated in normal and saline condition. The results indicated that in saline condition, total dry weight, root fresh weight, shoot dry weight, root/Shoot ratio, specific leaf weight, root length, proline and Na content were increased and the other traits were decreased. Study of correlation of traits showed that most significant difference between the two conditions was observed for the root/shoot ratio, so that, this trait has negative significant relation with total dry weight, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, relative water content, leaf area, root length, specific leaf weight in saline condition, but in normal condition correlation is positive and significant only in the total dry weight, root fresh weight, shoot dry weight and root dray weight and was not significant in the other traits. Step-wise regression analysis for total dry weight as dependent variable revealed that in normal condition, root fresh weight, shoot fresh weight and Na content expound of 93.1% and in saline condition root fresh weight, root length, Na content and proline explicate of 81.3% of total variation exist in total dry weight. Therefore, it is suggested to consider different traits in breeding programs for normal and saline conditions.


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