Advances in abiotic stress-resistant varieties of ornamentals

Author(s):  
Qiansheng Li ◽  
◽  
Mengmeng Gu ◽  
2016 ◽  
Vol 14 (1) ◽  
pp. 87-95
Author(s):  
Phạm Xuân Hội ◽  
Trần Thị Thúy Nga ◽  
Nguyễn Duy Phương

Plant improvement based on plant transformation technology has become a popular trend all over the world. The isolation and characterization of a complete set of genes/promoters associated with abiotic stress and the cross-talk among salinity, drought or temperature stresses are required for success in generating abiotic stress-resistant varieties. Recently, several studies on model plants demonstrated that the stress-responsive genes under the control of rice promoter Oshox24 were expressed specifically under stress conditions, while not affecting plant growth under normal conditions. In this study, we isolated DNA segments carrying the Oshox24 promoter from an Indica rice variety by PCR using specific primers. The Oshox24 promoter was cloned into the pGEM-T vector and fully sequenced. The nucleotide sequence of the isolated promoter Oshox24 was 1612 bp in size and had similarity of 90% in comparison to the Japonica rice promoter Oshox24 (published in GenBank as ID AP004868.3). Results of sequence analysis showed that the Indica Oshox24 promoter contains 15 stress-responsive cis-acting elements including 5 important groups ABRE, MYBRS/MYCRS, DRE, NACRS and ZFHDRS, and a TATA box located at position 1482 to 1488, suggesting that Oshox24 promoter plays an important role on regulation of abiotic stress inducible genes. The Oshox24 promoter was inserted into the expression vector pCAMBIA1300 for generating abiotic stress resistant plants in the future.


2018 ◽  
Vol 34 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Hemant B Kardile ◽  
◽  
Vikrant ◽  
Nirmal Kant Sharma ◽  
Ankita Sharma ◽  
...  

EDIS ◽  
2017 ◽  
Vol 2017 (6) ◽  
Author(s):  
Rebecca L. Wente ◽  
Samuel F. Hutton ◽  
Scott Adkins ◽  
William Turechek ◽  
Joseph E. Funderburk

Authors describe performance of Tospovirus-resistant tomato varieties under commercial production settings in Dade County, FL. Virus resistance has become important for this area since the emergence of two new tomato-infecting tospoviruses. Knowledge of availability and performance of resistant varieties will help growers who are looking for alternative varieties.


2020 ◽  
Vol 21 (1) ◽  
pp. 6-9
Author(s):  
Wuye Ria Andayanie

Soybean superior varieties with high yields and are resistant to abiotic stress have been largely released, although some varieties grown in the field are not resistant to SMV. In addition, the opportunity to obtain lines of hope as prospective varieties with high yield and resistance to SMV is very small. The method for evaluating soybean germplasm is based on serological observations of 98 accessions of leaf samples from SMV inoculation with T isolate. The evaluation results of 98 accessions based on visual observations showed 31 genotypes reacting very resistant or healthy to mild resistant category to SMV T isolate  with a percentage of symptom severity of 0 −30 %. Among 31 genotypes there are 2 genotypes (PI 200485; M8Grb 44; Mlg 3288) with the category of visually very resistant and resistant, respectively and  Mlg 3288  with the category of mild resistant.  They have a good agronomic appearance with a weight of 100 seeds (˃10 g) and react negatively with polyclonal antibodies to SMV, except Mlg 3288 reaction is not consistent, despite the weight of 100 seeds (˃ 10 g). Leaf samples from 98 accessions revealed various symptoms of SMV infection in the field. This diversity of symptoms is caused by susceptibility to accession, when infection occurs, and environmental factors. Keywords—: soybean; genotipe; Soybean mosaic virus (SMV); disease severity; polyclonal  antibody


2018 ◽  
Vol 1 (94) ◽  
pp. 45-50
Author(s):  
M.S. Korniychuk ◽  
N.V. Tkachenko

The expediency of fusarium resistant lupines usage in organic farming for improving soil fertility and producing high protein feed for livestock is justified in this article. Organic production excludes the use of mineral fertilizers and pesticides. In these circumstances, increases the value of cultures that positively affect the fertility of soils and their phytosanitary status. These crops include annual fodder lupine (yellow, white and angustifolia), especially varieties that are resistant to disease. Having the highest nitrogen-fixing ability among annual legume crops, lupins, depending on the type and conditions of cultivation, can accumulate from 80 to 300 kg/ha of environmentally pure symbiotic nitrogen in biomass. Plowing the green mass (30-45 t/ha) is equivalent to the corresponding amount of manure or introduction of 5-7 t/ha of ammonium nitrate. 30 kg/ha of phosphorus and 50 kg/ha of potassium are entering the soil with plant remains. Therefore, lupine provides itself with nutrients and still leaves up to 150 kg/ha of nitrogen in the soil after harvesting for subsequent crops in crop rotation. In organic production, fusariosis resistant varieties of lupines are also interesting as a source of high protein feeds for livestock. They are building up to 60 t/ha of biomass, which contains more than one ton of digestible protein. In grain of the fodder varieties contains 40-52% of crude protein, 5.5-6.0% of fat, 10-12% of sugar. The characteristic of fusarium resistant varieties of lupins (yellow, white and angustifolia), created in the NSC "Institute of Agriculture NAAS" and included in the Register of Plant Varieties of Ukraine is presented in this article. These varieties are practically not affected by fusariosis under the production conditions and do not require the use of fungicides during the growing season. They can be grown in crop rotation with a return period of 2-3 years, whereas for unstable it took 6-7 years. Because of the inability to use in organic farming fungicide to prevent crop losses from anthracnose, it is necessary to use predominantly angustifolia lupine varieties, which are now more tolerant to this disease, prevent sowing of infected seeds and adhere to crop rotation. Fusarium-resistant varieties of lupins are suitable for cultivation in poucous and sown crops, and in mixtures with other crops.


2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Ik-Young Choi ◽  
Prakash Basnet ◽  
Hana Yoo ◽  
Neha Samir Roy ◽  
Rahul Vasudeo Ramekar ◽  
...  

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.


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