scholarly journals The spectrophotometric determination of A-vitamin activity of carotenoid-containing raw material

2021 ◽  
pp. 17-26
Author(s):  
V.V. Velichko ◽  
◽  
D.S. Kruglov ◽  
Keyword(s):  
Spectrophotometric Determination ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Health Improvement ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Wide Range ◽  
Vitamin Activity

Carotenoids are of interest as precursors of vitamin A, which has a wide range of pharmacological effects — antioxidant, immunomodulatory, participating in photoreception, etc. The aim of the research was to develop a technique for the spectrophotometric determination of the A-vitamin activity of carotenoids-rich medicinal plant raw materials (MPRM). The objects of research were the traditional sources of carotenoids — sea buckthorn, carrot, pumpkin and wild rose. A method is proposed for determining the content of individual carotenoids in MPRM by optical density at wavelengths corresponding to the characteristic peaks of α-carotene, β-carotene and lycopene. It has been shown that it is preferable to use isopropyl alcohol as the most effective solvent. Taking into account the content of specific carotenoids makes it possible to determine the A-vitamin activity of the sum of carotenoids extracted from plant materials, and, accordingly, to increase the efficiency of the use of herbal preparations and biologically active additives for health improvement.

STUDY OF MORPHOMETRIC CHARACTERISTICS OF ALDER LEAVES OF PHARMACOPOEIAL SPECIES

2021 ◽  
pp. 129-134
Author(s):  
Nesterov G.V. ◽  
Litvinova T.M. ◽  
Vasalatiy L.A.
Keyword(s):  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Food Plants ◽  
Wild Plants ◽  
Material Base ◽  
Black Alder ◽  
Plant Raw Materials ◽  
Wide Range ◽  
Promising Area

To expand the raw material base of medicinal plant raw materials, a promising area of research is the study of additional types of raw materials that can be harvested from already used medicinal and food plants. Given the complexity of harvesting alder seeds, researchers are interested in the leaves of this plant, a wide range of biologically active substances that cause anti-inflammatory, astringent and antioxidant effects. The first stage of standardization of raw materials is the definition of morphologically significant features, based on the generalization of which the section "External features" is developed. The analysis of morphological features of freshly harvested, dried and frozen leaves of gray alder (Alnus incana Moench) and black alder (A. glutinosa Gaerth) harvested from wild plants in mixed forests of the Moscow region revealed diagnostically significant features and found that the method of conservation does not affect the occurrence of the identified features. Since it is planned to use both types of alder for the preparation of a new type of raw material "Alder leaves", a generalized formulation is proposed for raw materials that are a mixture of species.

scholarly journals Methods for the determination of flavonoids in plant raw materials and products of their processing

2020 ◽  
Author(s):  
N.V. Alpatova ◽  
◽  
I.A. Dubrovskaya ◽  
M.V. Slobodyanik ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Comparative Analysis ◽  
Literature Review ◽  
Raw Materials ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Plant Materials ◽  
Qualitative And Quantitative ◽  
Plant Raw Materials

Flavonoids are an important component of plant materials; they have medicinal and biologically active properties. To use them as medicines, it is necessary to determine their qualitative and quantitative composition. The aim of this work is a comparative analysis of the identification and metrological characteristics of methods for the determination of flavonoids in plant raw materials and products of their processing. The paper presents a brief literature review of the main methods for the study of flavonoids. Certified and advanced techniques, such as NMR and mass spectrometry, have been discussed.

scholarly journals PLANT-BASED MILK ANALOGUES IN THE NUTRITION OF PEOPLE WITH NUTRITION-DEPENDENT NON-INFECTIOUS DISEASES

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
N. Pritulska ◽  
I. Motuzka ◽  
A. Koshelnyk ◽  
M. Jarossová ◽  
A. Lacková
Keyword(s):  
Infectious Diseases ◽  
Raw Materials ◽  
Food Products ◽  
Domestic Market ◽  
Biologically Active ◽  
Raw Material ◽  
Fenugreek Seeds ◽  
Product Group ◽  
Wide Range ◽  
Treatment Procedures

A recent tendency in Ukraine is the changes in the structure of food consumption, which are caused by the reduced consumption of some product groups and by their decreasing quality. A persisting problem is that affordable and easy-to-consume food products made with due consideration of the needs of patients with certain categories of diseases can hardly be found on the domestic market. The products present on the domestic market are all imported, available in a limited assortment, very expensive, and not customised to suit all the specific needs of human metabolism. Approaches to the nutrition of patients with certain types of nutrition-dependent and non-infectious diseases have been analysed. It has been established that аn effective component in the diet of patients with non-infectious diseases can be plant-based milk analogues. It has been determined how important it is to use domestic raw materials: it will contribute to expanding domestic production, will help the gross domestic product growth, and will reduce the product’s costs, thus reducing the costs of diagnostic and treatment procedures. It has been established that the assortment of plant-based milk analogues is constantly expanding. The vegetable raw material conventionally used to make this product group has been analysed. It has been shown that fenugreek seeds can be most effectively used to make special food products (in particular, plant-based milk analogues) for the nutritional support of patients with non-infectious diseases. This is feasible due to the availability of the raw material, simple cultivating conditions, the chemical composition of the product (e. g. a wide range of biologically active substances), low costs of the product when it is made from domestic raw materials, and the simple production technology. It is expected that manufacture of milk analogues from fenugreek seeds will widen the assortment of this product group, and satisfy the target consumers’ needs of safe and high quality products that offer an alternative to imports.

scholarly journals Fast screening of some flavonoids content in raw plant materials: opportunities of 1H NMR spectroscopy

2020 ◽  
Vol 169 ◽  
pp. 02006
Author(s):  
Gennady Kalabin ◽  
Vasilii Vasil’ev ◽  
Vasilii Ivlev ◽  
Vasilii Babkin
Keyword(s):  
Nmr Spectroscopy ◽  
Plant Biomass ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Standard Samples ◽  
Plant Materials ◽  
H Nmr ◽  
Fast Screening ◽  
Raw Plant Materials

Environmental monitoring and assessment of the prospects for extracting biologically active substances (BAS) from various types of plant biomass requires the development of simple and fast methods for measuring their content in raw materials. A new approach for measuring the content of various flavonoids groups in plant raw material using 1H NMR spectroscopy has been developed, which allows to characterize its resource capabilities and study the effects on their composition different environmental factors without complex sample preparation and standard samples.

scholarly journals Experimental Determination of Biologically Active Compounds in Pomace of Siberian Beet and Carrot

2021 ◽  
Vol 51 (1) ◽  
pp. 179-187
Author(s):  
Anastasia Kozhemayko ◽  
Irina Sergeeva ◽  
Irina Dolgolyuk
Keyword(s):  
Functional Food ◽  
Nutritional Value ◽  
Raw Materials ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Positive Trend ◽  
Bioactive Substances ◽  
Industrial Processing

Introduction. With the development of the food and processing industry, the matter of environmental pollution is becoming more and more acute. Environmental protection is based on the principle of rational use of natural resources and sustainable technology. Vegetable pomace is a secondary raw material; its amount depends on the production technology and equipment. The observed positive trend in the gross harvest of vegetables in open ground can increase the number of vegetable processing enterprises and the capacity of existing enterprises. Eventually, waste will start accumulating at processing sites, and it will have to be used as raw materials. The present paper features the content of biologically active substances in pomace of carrots and beets grown on the territory of the Siberian region and introduces options for their further use in functional foods. Study objects and methods. The research featured carrot pomace of the varieties Losinoostrovskaya, Nantskaya, and Queen of Autumn, as well as beet pomace of varieties Cylinder and Bordeaux. All the samples were harvested in the Kemerovo region in 2019. Determination of physical and chemical parameters was carried out using standard methods. Carotenoids, flavonoids, β-cyanine were studied using spectrometry and photocolorimetric method. Results and discussion. The experiment featured the content of bioactive substances in pomace of carrots and beets obtained during industrial processing. The content of carotenoids in carrots (mg of β-carotene per 100 g of dry weight): for Losinoostrovskaya variety – 23.56 ± 0.23; Nantskaya – 25.32 ± 0.18; Queen of Autumn – 20.78 ± 0.25. Flavonoid content (mg of catechol equivalent per 100 g of dry weight): Losinoostrovskaya – 12.02 ± 0.37; Nantskaya – 13.45 ± 0.56; Queen of Autumn – 11.50 ± 0.48. The content of β-cyanine in beets (mg per 100 g of dry weight): Cylinder – 100.0 ± 8.5; Bordeaux – 35.0 ± 1.8. The nutritional value of carrot and beet pomace with a mass fraction of moisture was 10%. The nutritional value of vegetable pomace is due to the high content of dietary fiber; therefore, the raw materials can be considered for functional food production. The content of biologically active substances in vegetable pomace (flavonoids, carotenoids, β-cyanines) can enhance the functional orientation of this secondary raw material when used in food technologies for the production of food of high nutritional value. Conclusion. The results obtained will make it possible to use the biochemical potential of plant raw materials in many aspects, as well as to obtain new functional food products, thereby expanding the range of healthy foods.

scholarly journals QUALITY AND NUTRITIONAL VALUE OF FREEZED SEA BUCKTHORN POWDER

2021 ◽  
pp. 7-14
Author(s):  
O. M. Burmistrova ◽  
E. A. Burmistrov ◽  
N. L. Naumova
Keyword(s):  
Nutritional Value ◽  
Raw Materials ◽  
Food Additives ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Raw Material ◽  
Test Material ◽  
Plant Raw Materials ◽  
Organoleptic Characteristics ◽  
Unique Trace

The use of phytopowders as food additives allows you to create highly nutritious mixtures with a pronounced taste and aroma of fresh fruits and berries. Fruit and berry powders are already used in the production of jelly, fruit drinks, drinks, juices and sauces based on domestic plant raw materials with a high proportion of biologically active substances. The most important phytonutrients of sea buckthorn fruits (Hippophae rhamnoides L.) are carotenoids, flavonoids, anthocyanins, sugars, organic and amino acids, tannins and pectin substances, phospholipids, macro- and microelements. The aim of the research was to study the quality and nutritional value of freezedried sea buckthorn powder. Sublimated sea buckthorn was the object of study (TU 10.39.25-007-0111115841-2018) manufactured by IP A.N. Mazurina (Russia, Kaluga region, Borovsky district, Borovsk). The quality of packaging and labeling, as well as organoleptic characteristics, nutritional value and mineral composition of raw materials have been studied. It was determined that the marking of sublimated sea buckthorn does not comply with the regulated requirements of GOST R 51074-2003 and TR CU 022/2011. Organoleptic characteristics of phytopowder are identified as characteristic of this type of raw material. The actual protein and fat content of the test material does not correspond to the levels stated by the manufacturer. The studied raw materials are distinguished by a variety of chemical composition, namely, the content of protein, carotenoids, vitamins E and A, lipids, dietary fiber, unique trace elements – Al, Co, Cr, Cu, Fe, Li, Mn, Ni, Ti, Zn.

scholarly journals Comparative study of the elemental composition in raw materials of plants of genus Geum L.

2021 ◽  
pp. 70-77
Author(s):  
С. А. Козира ◽  
Т. М. Гонтова ◽  
С. І. Степанова ◽  
В. П. Гапоненко ◽  
В. В. Машталер ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Comparative Study ◽  
Atomic Absorption ◽  
Elemental Composition ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Material Base ◽  
Plant Raw Materials ◽  
Microelement Composition

Among the relevant for the modern pharmacy problems, there is a need for the rational complex use of known medicinal plants, as well as the search for new sources of natural biologically active compounds in order to expand the list of official medicinal plants and raw material base. Species of genus Geum L. contain a number of biological active substances and are promising sources of raw materials for the production of antimicrobial, astringent, anti-inflammatory, and hemostatic medicines. Pharmacological activity of these plants results from their multi-component chemical composition, including tannins, flavonoids, macro- and microelements and other compounds. The aim of thе study was to compare the elemental composition of herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. of Ukrainian flora. The objects of the study were the samples of herb and rhizome with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. (dried raw materials) which were collected in the Kharkiv region, Ruski Tishki village, in June-August 2018–2019. The determination of the qualitative composition and quantitative content of elements was carried out by atomic absorption spectroscopy with atomization in an air-acetylene flame using a spectrometer CAS 120 («Selmi», Sumy, Ukraine). In the studied samples, 19 elements were determined, including 5 macroelements and 14 microelements. The data about accumulation of elements in investigated herb of G. urbanum L., G. aleppicum Jacq., G. rivale L. can be arranged in descending order as follows: K > Ca > Si > Mg > P > Fe > Al > Na > Sr > Zn, and in rhizomes with roots as follows – K > Si > Ca > Mg > Fe > P > Al > Na > Sr > Zn. The content of toxic elements such as cobalt, cadmium, arsenic, and mercury was within the permissible limits for plant raw materials and food. For the first time, a comparative study of the macro- and microelement composition in herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. has been carried out. Using the method of atomic absorption spectral analysis, 19 elements have been identified and their content has been determined, among which К, Ca, Mg, Si, P, Fe, Al, Na, Mn, Zn predominantly accumulate.

scholarly journals Members of the family Lamiaceae Lindl. as sources of medicinal plant raw materials to obtain neurotropic drugs

2020 ◽  
Vol 8 (1) ◽  
pp. 4-28
Author(s):  
Е. V. Zvezdina ◽  
J. V. Dayronas ◽  
I. I. Bochkareva ◽  
I. N. Zilfikarov ◽  
E. Yu. Babaeva ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Scientific Literature ◽  
Raw Materials ◽  
Neurotropic Activity ◽  
Biologically Active ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Pharmaceutical Substances ◽  
The Family ◽  
Active Substances

The aim of this work is to review and analyze the data published in the modern scientific literature obtained in pharmacological, pharmacognostic and pharmacotechnological studies of various types of raw materials obtained from members of the family Lamiaceae L., which were sources of biologically active substances, pharmaceutical substances, total extracts and the drugs – with a neurotropic activity.Materials and methods. For the review, we used the information of scientific literature from open and accessible sources of the last twenty years, located in the scientific and technical libraries of institutions, as well as in electronic databases: Elibrary, PubMed, Scopus, Cyberleninka, GoogleAcademy, J-Stage. The search inquiries were: the species of the family Lamiaceae (Russian and Latin), the samples of medicinal plant materials based on them as well as the names of the drugs and biologically active substances obtained from these raw materials.Results. When working with the sources of scientific information, the main attention was paid to pharmacologic tests performed during the studies on laboratory animals and proving the presence of neurotropic activity in the studied objects – essential oils and extracts from plant raw materials: aqueous, aqueous alcoholic, and methanol ones. It has been established that the potential of the therapeutic and preventive application of pharmaceutical substances and drugs based on the medicinal plant materials obtained from 30 genera members of the Lamiaceae family, remains unrealized despite the close attention of various researchers.Conclusion. This review comprised 71 species from 30 genera. Despite the significant level of the previous study presented in the analysis of this publication, an enormous potential of this family’s species remains unexplored. In the future, they can be of both – pharmacognostic and practical interest, in particular, in creation of new medicinal preparations of the neurotropic action based on them.

scholarly journals Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.

2020 ◽  
Vol 9 (4) ◽  
pp. 107-114
Author(s):  
A. E. Sukhanov ◽  
A. N. Stavrianidi ◽  
E. D. Kubasova ◽  
A. S. Panasyuk ◽  
O. V. Buyuklinskaya
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Plant Extracts ◽  
Raw Materials ◽  
Biologically Active ◽  
Fenugreek Seeds ◽  
Plant Raw Materials ◽  
Object Of Study ◽  
Trigonella Foenum Graecum L

Introduction. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts.Aim. Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds.Materials and methods. The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017.Results and discussion. Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed.Conclusion. It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.

TECHNOLOGY OF PRODUCTION OF BIOLOGICALLY ACTIVE PLANT COMPLEX: DETERMINATION OF REGULATED PARAMETERS, FUNCTIONAL PROPERTIES

2019 ◽  
Keyword(s):  
Functional Properties ◽  
Viral Infections ◽  
Raw Materials ◽  
Extraction Process ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Comprehensive Treatment ◽  
Plant Raw Materials ◽  
Technology Of Production

The technology of production of a biologically active phytocomplex in the form of a biologically active additive has been discussed. The qualitative and quantitative composition of the formulation, which forms the functional properties of a specialized product, has scientifically been determined. The main stages of production include the preparation and input control of the raw material, the preparation of a mixture for encapsulation, including the steps of dispensing, sieving, grinding and mixing at a rate of 100 kg / 1 h, encapsulation and deducting, packaging, packaging and storage. A description of the technology of ex-traction of plant raw materials has been given, which is of great importance in the formation of consumer properties of the product being developed. The extraction process includes: obtaining liquid recovery us-ing demineralized water with dissolved in it sodium bicarbonate in an amount of 8% of the dry feed con-tent. The extractant-raw ratio is 1:13, the extraction time is 4 hours for each time; thickening of the liquid extract. It is carried out by means of vacuum in a vacuum evaporator plant before obtaining an extract with a solids content of not less than 20%; obtaining a dry extract. It is carried out by spray drying at a temperature of 90-95 ° C. The advantage of the developed technology of dietary supplements is the possi-bility of combining several differently directed biologically active substances in one capsule. The gelati-nous membrane reliably protects the contents from various environmental factors. Functional properties of the formulation formula of a specialized product are aimed at the prevention and comprehensive treatment of infectious viral diseases, confirmed by the conclusion of experts from the Russian Federal Service for Consumer Rights and Consumer Protection and the results of clinical trials in a group of pa-tients with acute respiratory viral infections. The developed product has been approved and produced at the enterprises of the company «Art Life».

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