scholarly journals Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.

2020 ◽  
Vol 9 (4) ◽  
pp. 107-114
Author(s):  
A. E. Sukhanov ◽  
A. N. Stavrianidi ◽  
E. D. Kubasova ◽  
A. S. Panasyuk ◽  
O. V. Buyuklinskaya
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Plant Extracts ◽  
Raw Materials ◽  
Biologically Active ◽  
Fenugreek Seeds ◽  
Plant Raw Materials ◽  
Object Of Study ◽  
Trigonella Foenum Graecum L

Introduction. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts.Aim. Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds.Materials and methods. The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017.Results and discussion. Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed.Conclusion. It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.

scholarly journals Isolation and determination of some physical and chemical properties of polysaccharide from black elderberry flowers

2020 ◽  
pp. 95-101
Author(s):  
Игорь Анатольевич Сычев ◽  
Татьяна Викторовна Алимкина
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Plant Raw Materials ◽  
Physical And Chemical ◽  
And Chemical Properties

Предложенный метод количественного определения восстанавливающих моносахаридов в полисахариде позволяет проводить стандартизацию лекарственного растительного сырья цветков бузины черной. На способ стандартизации лекарственного растительного сырья цветков бузины черной по содержанию восстанавливающих моносахаридов в полисахариде оформлено рационализаторское предложение № 1373 от 29.02.2016 г. РязГМУ. The proposed method for the quantitative determination of reducing monosaccharides in a polysaccharide makes it possible to standardize medicinal plant raw materials of black elderberry flowers. On the method of standardization of medicinal plant raw materials of black elderberry flowers in terms of the content of reducing monosaccharides in the polysaccharide, a rationalization proposal No. 1373 dated February 29, 2016 RyazSMU was issued.

scholarly journals METHODS FOR QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN QUERCUS ROBUR L. BUDS

2021 ◽  
Vol 9 (5) ◽  
pp. 356-366
Author(s):  
N. A. Ryabov ◽  
V. M. Ryzhov ◽  
V. A. Kurkin
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Ethyl Alcohol ◽  
Quercus Robur ◽  
Raw Materials ◽  
Uv Spectrophotometry ◽  
Total Flavonoids ◽  
Plant Raw Materials ◽  
Wide Range

Currently, the actual task of modern pharmacy is to study the chemical composition and pharmacological properties of plant objects. Within the framework of this concept, it seems interesting to study Quercus robur L. buds. One of the promising groups of biologically active compounds of Quercus robur L. buds are flavonoids. This group of substances has a wide range of a pharmacological activity, which is significant in the creation of new medicines based on medicinal plant raw materials.The aim of the article was to work out methods for quantitative determination of total flavonoids in Quercus robur l. buds.Materials and methods. The research materials were aqueous-alcoholic extracts from Quercus robur L. buds with 70% ethyl alcohol which were analyzed by differential UV spectrophotometry on spectrophotometer “SF 2000” (Russia).Results. The methods for quantitative determination of total flavonoids in Quercus robur L. buds by differential UV spectrophotometry, has been developed using a standard sample of cynaroside at the analytical wavelength of 400 nm. The optimum parameters for the extraction of total flavonoids from Quercus robur L. buds have been determined. They are: the optimum extractant is 70% ethyl alcohol; the “raw material-extractant” ratio is 1:50; the extraction time is 120 min, the degree of atomization is 2 mm.The content of total flavonoids for Quercus robur L. buds has been determined; it varies from 0.27%±0.01 to 0.44%±0.02. These results make possible to recommend the content of total flavonoids for this type of raw materials not less than 0.25% as a lower limit.Conclusion. The data obtained in the course of the experiment, makes it possible to conclude that a further study of Quercus robur L. buds is promising, and it also contributes to the implementation of medicinal plant raw materials “Quercus robur L. buds” in the State Pharmacopoeia (Russia).

scholarly journals Development of approaches to standardization of black walnut bark

2020 ◽  
Vol 20 (1-2) ◽  
pp. 131-136
Author(s):  
Natalya I. Zimenkina ◽  
Vladimir A. Kurkin
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Juglans Regia ◽  
Black Walnut ◽  
Juglans Nigra ◽  
Raw Material ◽  
Plant Raw Materials ◽  
Plant Raw Material

Significance. Black Walnut (Juglans nigra L.) is a species of trees of the Walnut family (Juglandaceae). This plant of the genus Walnut (Juglans L.) has not been sufficiently studied unlike other species e.g. Juglans regia L. This medicinal plant raw material is quite perspective, its preparations have antimicrobial, general tonic effect. We can use its leaves, unripe fruit, and the bark. However they are not widely used in medicine. In order to introduce the plants of the Walnut genus to the State Pharmacopoeia of the Russian Federation (RF State Pharmacopoeia), it is necessary to conduct of pharmacognostic studies, to develop product specification file to confirm the identification and quality of medicinal plant raw materials. The aim of this study is to develop a method of quantitative determination of flavonoids in the bark of the black walnut (Juglans nigra L.). Materials and methods. Material of the study was black walnut bark, stocked during the sap flow period (April) in 2018. The bark was skived up to 15 cm long and 23 cm wide. The bark was air-dried with the protection from direct sun light. The end of the drying was checked by the brittleness of the bark. Results. The methods of the quantitative determination of flavonoids in walnut bark has been developed. We used the differential spectrophotometry taking into consideration state standard sample of rutin at the analytical wavelength of 416 nm. The error of a single determination with a confidence level of 95% is 1.20%. Conclusion. We used the developed technique and analyzed a number of samples of black walnut bark. The content of total flavonoids in the plant raw material is 5.13 0.02% (as calculated on rutin). The flavonoid content should be at least 4.0%.

scholarly journals DEvELopMENT of TEOHNiQuEs FoR THE DETERMiNATioN oF MoisTuRE (Loss oN DRYING) oF MEDiOiNAL PLANT RAW MATERiALS oF MoRPHoLoGiOAL group “FRUiT” by infrared thermogravimetric method

2019 ◽  
Vol 19 (1-2) ◽  
pp. 13-18
Author(s):  
D. A Zhdanov ◽  
V. B Braslavsky
Keyword(s):  
Medicinal Plant ◽  
Russian Federation ◽  
Raw Materials ◽  
The State ◽  
Moisture Loss ◽  
Anethum Graveolens ◽  
Thermogravimetric Method ◽  
Plant Raw Materials ◽  
The Russian Federation

The article is devoted to the development of new and perspective for the State Pharmacopoeia of the Russian Federation (SP RF) techniques for determining of the moisture (loss on drying) of medicinal plant raw materials of the morphological group “Fruit” and medicinal plant preparations by the use of infrared thermogravimetric (IRTGM) method. The techniques for determination of moisture (loss on drying) IRTGM-method as exemplified by the fruit of the following medicinal plants: Silybum marianum (L.) Gaertn., Rosa cinnamomea L. and Anethum graveolens L. were developed. The comparable results for determining of the moisture (loss on drying) of the medicinal plant raw materials of investigated plants were obtained by means of two methods: IRTGM and pharmacopoeial method, which allow us to recommend the developed techniques for inclusion into the relevant monographs of the State Pharmacopoeia of the Russian Federation.

scholarly journals Members of the family Lamiaceae Lindl. as sources of medicinal plant raw materials to obtain neurotropic drugs

2020 ◽  
Vol 8 (1) ◽  
pp. 4-28
Author(s):  
Е. V. Zvezdina ◽  
J. V. Dayronas ◽  
I. I. Bochkareva ◽  
I. N. Zilfikarov ◽  
E. Yu. Babaeva ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Scientific Literature ◽  
Raw Materials ◽  
Neurotropic Activity ◽  
Biologically Active ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Pharmaceutical Substances ◽  
The Family ◽  
Active Substances

The aim of this work is to review and analyze the data published in the modern scientific literature obtained in pharmacological, pharmacognostic and pharmacotechnological studies of various types of raw materials obtained from members of the family Lamiaceae L., which were sources of biologically active substances, pharmaceutical substances, total extracts and the drugs – with a neurotropic activity.Materials and methods. For the review, we used the information of scientific literature from open and accessible sources of the last twenty years, located in the scientific and technical libraries of institutions, as well as in electronic databases: Elibrary, PubMed, Scopus, Cyberleninka, GoogleAcademy, J-Stage. The search inquiries were: the species of the family Lamiaceae (Russian and Latin), the samples of medicinal plant materials based on them as well as the names of the drugs and biologically active substances obtained from these raw materials.Results. When working with the sources of scientific information, the main attention was paid to pharmacologic tests performed during the studies on laboratory animals and proving the presence of neurotropic activity in the studied objects – essential oils and extracts from plant raw materials: aqueous, aqueous alcoholic, and methanol ones. It has been established that the potential of the therapeutic and preventive application of pharmaceutical substances and drugs based on the medicinal plant materials obtained from 30 genera members of the Lamiaceae family, remains unrealized despite the close attention of various researchers.Conclusion. This review comprised 71 species from 30 genera. Despite the significant level of the previous study presented in the analysis of this publication, an enormous potential of this family’s species remains unexplored. In the future, they can be of both – pharmacognostic and practical interest, in particular, in creation of new medicinal preparations of the neurotropic action based on them.

scholarly journals Flavonoid’s Content Determination in Irradiated Samples via Spectrophotometric Analysis

2019 ◽  
Vol 5 (10) ◽  
pp. 32-39 ◽  
Author(s):  
A. Sarukhanov ◽  
A. Morozova ◽  
N. Vasilyeva ◽  
D. Krylenkin
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
General Information ◽  
Spectrophotometric Analysis ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Qualitative And Quantitative ◽  
Content Determination ◽  
Active Substances

General information about biologically active substances flavonoids is concisely presented. The need for the quantitative determination of its content in products and the influence of product processing on flavonoid’s content is justified. Methods of qualitative and quantitative determination of flavonoids content in various products, medications and raw materials were studied. Based on this study the method of quantitative determination of flavonoid’s in samples of irradiated coriander is compiled and tested. Further ways to improve and adjust the compiled method for flavonoid’s content determination in irradiated samples via spectrophotometric analysis is considered.

scholarly journals THE DEVELOPMENT OF METHOD OF QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN THE FLOWERS OF CRATAEGUS SUBMOLLIS SARG.

2019 ◽  
pp. 137-144 ◽  
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Yevgen'yevna Pravdivtseva ◽  
Tat'yana Vladimirovna Morozova ◽  
Anna Vladimirovna Kurkina ◽  
Il'nur Khyasyainovich Shaikhutdinov ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Specific Weight ◽  
Raw Material ◽  
High Yield ◽  
Total Flavonoids ◽  
Plant Raw Materials ◽  
The Russian Federation ◽  
Promising Type

The flowers and fruits of various species of the hawthorn genus (Crataegus L.) are widely used in domestic and foreign medical practice as cardiotonic preparations. In our opinion, Crataegus submolls Sarg. is a perspective species for collection of the raw materials. Crataegus submolls Sarg. is successfully cultivated in the Russian Federation and is distinguished by its rapid growth and high yield compared with wild-growing species. Moreover, the fresh fruits of Crataegus submolls Sarg. are used in nutrition. The flowers of Crataegus submollis Sarg. contain flavonoids, among of which, like in the case of Hawthorn blood-red the hyperoside is present. It was determined that the maximum of the absorption curve of the solution of Crataegus submollis Sarg. flowers with a solution of aluminum chloride is 412 nm. The method of quantitative determination by the differential spectrophotometry of the total flavonoids calculated on hyperoside in the Crataegus submollis Sarg. flowers has been developed. It was established that the appropriate extragent for this raw material is 70% ethanol. The level of total flavonoid in Crataegus submollis Sarg. flowers has been studied. It was established that separate parts of Crataegus submollis Sarg. flowers differ markedly in the content of total flavonoids and their specific weight in raw materials. It was established that the content of total flavonoids calculated on hyperoside is varied from 2.05±0.08% tо 3.00±0.12%. It was determined that the content of total flavonoids calculated on hyperoside in different parts of hawthorn flowers ranges from 0.98% (receptacle and calyx) to 4.53% (petals). In our opinion, Crataegus submollis Sarg. flowers are the promising type of medicinal plant raw materials.

scholarly journals IMPROVING THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE RAW MATERIAL “ROSEHIP FRUIT”

2020 ◽  
pp. 131-138
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Vladimirovna Sharova ◽  
Polina Valeriyevna Afanaseva
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Water Soluble ◽  
Biologically Active ◽  
Raw Material ◽  
Test Quality ◽  
Total Carotenoids ◽  
Long Time

The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves. Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times. We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.

DEVELOPMENT OF THE COMPOSITION AND STANDARTIZATION OF THE COLLECTION FOR THE PREVENTION OF INFLAMMATORY PERIODONTAL DISEASES

2021 ◽  
pp. 73-79
Author(s):  
Groznov A.A. ◽  
Kachulin V.A. ◽  
Nesterova O.V.
Keyword(s):  
Essential Oil ◽  
Medicinal Plant ◽  
Quantitative Determination ◽  
Periodontal Diseases ◽  
Antimicrobial Effect ◽  
Biologically Active ◽  
Extractive Substances ◽  
Definition Of ◽  
Active Substances

Taking into account the literature data, as well as the research conducted by the authors, the composition of the collection for the prevention of inflammatory periodontal diseases has been developed, which includes medicinal plant raw materials:medicinal pimenta fruits, medicinal sage leaves, yarrow grass, badan rhizomes in a ratio of 1:1:1: 1 by weight. The quantitative ratio of the components is determined in accordance with the results of the evaluation of the antimicrobial effect of the extract from the proposed collection. To confirm the composition of biologically active substances of the collection, pharmacopoeial qualitative reactions to flavonoids and tannins, as well as the TLC method, which allows identifying substances of a polyphenolic nature, are proposed. The quantitative determination of extractive substances, essential oil, tannins, and flavonoids in the studied collection was carried out and the norms of their content were proposed. The definition of commodity characteristics is carried out.

SELECTION OF COMPOSITION AND TECHNOLOGY OF GRANULES WITH GARDEN LEAVE STRAWBERRY EXTRACT DRY

2021 ◽  
pp. 154-161
Author(s):  
Yaborova O.V. ◽  
Zamakhaeva E.A. ◽  
Oleshko O.A.
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Food Product ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Leaf Extracts ◽  
Optimal Method ◽  
Active Substances

Garden strawberries (Fragaria ananassa) are widely cultivated on the territory of Russia on industrial plantations and private garden plots to obtain the fruits (berries) of this plant and their use as a valuable food product. When harvesting fruits, other parts of the garden strawberry are not used, however, its vegetative parts are a source of biologically active substances (BAS) and are of interest for the pharmaceutical and cosmetic industries [1, 4]. The biological activity of garden strawberry leaf extracts is due to the presence of secondary metabolites of a phenolic nature; their content in raw materials reaches more than 3% in some samples [2, 3]. Considering that the main group of biologically active substances of this raw material are flavonoids, the Department of Pharmacognosy with a course of botany conducted research to develop a method for quantitative determination of the amount of flavonoids in terms of rutin. Spectrophotometry was chosen as the optimal method for assessing the content of this group of substances in medicinal plant raw materials - leaves of garden strawberries, which allows determining the amount of flavonoids in terms of the dominant compound and obtaining reliable and reproducible results of quantitative analysis [8, 11]. The technological properties were studied and a therapeutic dose of dry strawberry extract was proposed, optimal compositions were selected and technologies of sachet-granules with dry strawberry extract were developed. The method for the quantitative determination of flavonoids in terms of rutin was validated. The results of the study can be used in the future in the development of draft regulatory documents for sachet-granules with dry strawberry extract of garden leaves and the study of their pharmacological activity.

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