scholarly journals In vitro evaluation of anti-adherence activity of the Ocimum basilicum l. (basil) extract and of the Coriandrum sativum l. (coriander) in acrylic surface of removable orthodontic appliances

2015 ◽  
Vol 3 (2) ◽  
pp. 636
Author(s):  
Fabíola Iahn Ferreira ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Eloisa Marcantonio Boeck
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Orthodontic Appliances ◽  
Bacterial Films ◽  
In Series ◽  
Glucan Synthesis ◽  
Removable Orthodontic Appliances

AIM: The aim of this study is evaluate the antimicrobial activity of the Ocimum basilicum L. (basil) extract  and of the Coriandrum sativum L. (coriander) extract in biofilm of Streptococcus mutans colonized in specimens confectioned in acrylic used to confection removable orthodontic appliances. MATERIAL AND METHODS: To perform this work, specimens were confectioned (spherical discs in sterile acrylic) which were immersed in extracts pure and in series dilutions (1:2 a 1:128) by 24, 48 and 72 hours. For each time of exposition, the disintegration of bacterial films was performed by sonication in saline solution. RESULTS: The results obtained were satisfactory for the extracts studied on the inhibition of biofilm formation. CONCLUSION: We conclude that polyphenols present in the extracts interfered on the glucan synthesis engine, possibly inhibiting the enzymes (glucosyl- and fructosyl transferase) which synthesize the extracellular polymers, also acting as antioxidant, and therewith they presented antimicrobial activity.

scholarly journals Evaluation in vitro of antimicrobial activity of basil extract (Ocimum basilicum L.) on an acrylic surface of removable orthodontic appliances

2014 ◽  
Vol 2 (3) ◽  
pp. 227
Author(s):  
Jéssica Fernanda Sedenho ◽  
Vanessa Ferreira Moraes ◽  
Adilson César de Abreu Bernardi ◽  
Nadia Lunardi ◽  
Eloisa Marcantonio Boeck
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Quantitative Analysis ◽  
Ocimum Basilicum ◽  
Orthodontic Appliances ◽  
Inhibition Control ◽  
Bacterial Film ◽  
Removable Orthodontic Appliances ◽  
Chlorhexidine Solution

AIM: The aim of this study was to evaluate in vitro the antimicrobial activity of Ocimum basilicum L. (basil) extract in S. Muttans biofilm colonized in specimens confectioned in the same acrylic used for removable orthodontics appliances. MATERIAL AND METHODS: To perform this work, 42 specimens were confectioned (sterile spherical acrylic disks) and immersed in pure extract and in serial dilution of extract (1:2 until 1:10) during 24, 48 and 72 hours. For each time of exposition, a disintegration of bacterial film by sonication in saline and posterior seed in agar, for colony count, were carried out. ATCC strains of S. muttans were selected, and 2% chlorhexidine solution was used as inhibition control. RESULTS: The results showed, by quantitative analysis, that basil extract has antibacterial activity in S. muttans biofilm, when used in pure state or in dilution until 1:4. CONCLUSION: Besides, it was possible observe the more increase the incubation time, independent the dilution, the higher degradation of extract.

scholarly journals In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

2018 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Mariana Lourenço ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Patricia Sigilló Mazzoni Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites

2020 ◽  
Vol 120 ◽  
pp. 104943
Author(s):  
Elizabeth Madla-Cruz ◽  
Myriam De la Garza-Ramos ◽  
César I. Romo-Sáenz ◽  
Patricia Tamez-Guerra ◽  
Marco A. Garza-Navarro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Carboxymethyl Cellulose ◽  
Biofilm Formation ◽  
Cellulose Composites ◽  
Human Dentine

scholarly journals In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 352
Author(s):  
Jan-Luca Schmid ◽  
Martin Kirchberg ◽  
Sandra Sarembe ◽  
Andreas Kiesow ◽  
Anton Sculean ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Gingival Crevicular Fluid ◽  
Oral Bacteria ◽  
Periodontal Therapy ◽  
Lipid Complex ◽  
Colony Forming Units ◽  
Crevicular Fluid

Periodontal therapy using antimicrobials that are topically applied requires slow or controlled release devices. The in vitro antimicrobial activity of biodegradable polymer formulations that contain a new minocycline lipid complex (P-MLC) was evaluated. The new P-MLC formulations that contained 11.5% minocycline were compared with pure minocycline or an existing commercial formulation, which included determination of minimal inhibitory concentration (MIC) values against two oral bacteria and activity on six-species periodontal biofilm. Moreover, the flow of gingival crevicular fluid (GCF) was modeled up to 42 days and the obtained eluates were tested both for MIC values and inhibiting biofilm formation. In general, MICs of the P-MLC formulations were slightly increased as compared with pure minocycline. Biofilm formation was clearly inhibited by all tested formulations containing minocycline with no clear difference between them. In 3.5 day old biofilms, all formulations with 250 µg/mL minocycline decreased bacterial counts by 3 log10 and metabolic activity with no difference to pure antimicrobials. Eluates of experimental formulations showed superiority in antimicrobial activity. Eluates of one experimental formulation (P503-MLC) still inhibited biofilm formation at 28 days, with a reduction by 1.87 log10 colony forming units (CFU) vs. the untreated control. The new experimental formulations can easily be instilled in periodontal pockets and represent alternatives in local antimicrobials, and thus warrant further testing.

scholarly journals In Vitro Study of Antimicrobial Percutaneous Nephrostomy Catheters for Prevention of Renal Infections

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Nylev Vargas-Cruz ◽  
Ruth A. Reitzel ◽  
Joel Rosenblatt ◽  
Mohamed Jamal ◽  
Ariel D. Szvalb ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Percutaneous Nephrostomy ◽  
Multidrug Resistant ◽  
Gram Positive ◽  
Gram Negative ◽  
Content Type ◽  
Fungal Organisms

ABSTRACT Percutaneous nephrostomy (PCN) catheters are the primary method for draining ureters obstructed by malignancy and preventing a decline of renal function. However, PCN catheter-related infections, such as pyelonephritis and urosepsis, remain a significant concern. Currently, no antimicrobial PCN catheters are available for preventing infection complications. Vascular catheters impregnated with minocycline-rifampin (M/R) and M/R with chlorhexidine coating (M/R plus CHD) have previously demonstrated antimicrobial activity. Therefore, in this study, we examined whether these combinations could be applied to PCN catheters and effectively inhibit biofilm formation by common uropathogens. An in vitro biofilm colonization model was used to assess the antimicrobial efficacy of M/R and M/R-plus-CHD PCN catheters against nine common multidrug-resistant Gram-positive and Gram-negative uropathogens as well as Candida glabrata and Candida albicans. Experimental catheters were also assessed for durability of antimicrobial activity for up 3 weeks. PCN catheters coated with M/R plus CHD completely inhibited biofilm formation for up to 3 weeks for all the organisms tested. The reduction in colonization compared to uncoated PCN catheters was significant for all Gram-positive, Gram-negative, and fungal organisms (P < 0.05). M/R-plus-CHD PCN catheters also produced significant reductions in biofilm colonization relative to M/R PCN catheters for Enterobacter spp., Escherichia coli, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, C. glabrata, and C. albicans (P < 0.05). M/R-plus-CHD PCN catheters proved to be highly efficacious in preventing biofilm colonization when exposed to multidrug-resistant pathogens common in PCN catheter-associated pyelonephritis. M/R-plus-CHD PCN catheters warrant evaluation in a clinical setting to assess their ability to prevent clinically relevant nephrostomy infections.

scholarly journals Packaging properties and control of Listeria monocytogenes in bologna by cellulosic films incorporated with pediocin

2013 ◽  
Vol 16 (3) ◽  
pp. 226-235 ◽  
Author(s):  
Paula Judith Pérez Espitia ◽  
Jhon Jairo Reina Pacheco ◽  
Nathália Ramos de Melo ◽  
Nilda de Fátima Ferreira Soares ◽  
Alba Manuela Durango
Keyword(s):  
Antimicrobial Activity ◽  
Water Vapor ◽  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Testing Machine ◽  
Meat Products ◽  
Gravimetric Method ◽  
Water Vapor Permeability ◽  
Vapor Permeability

Listeria monocytogenes is a foodborne pathogen, able to survive and proliferate at refrigeration temperatures. As a result, ready-to-eat meat products have been associated with major outbreaks. Producing meat products involves lethal preservation treatments, e.g. thermal treatments. Listeria contamination, however, may be introduced when products are sliced and packaged at retail businesses or delicatessens. In Brazil, sliced bologna is very popular at retail markets. After slicing, however, bologna has a short shelf-life. The aim of this work was to study the effects of pediocin incorporation on the load at break, water vapor permeability rate and structure, by microscopic analysis, of antimicrobial cellulosic packaging. The potential application of the developed packaging for the preservation of bologna and inhibition of Listeria biofilm formation was also studied. Cellulosic antimicrobial packaging films were produced with cellulose acetate and acetone. Pediocin (commercially available concentrate ALTA TM 2341) was incorporated at 30, 40 and 50 % w/w. The load at break of films was studied using the Universal Testing Machine (Instron) at 10 °C and 25 °C. The water vapor permeability was determined by gravimetric method. A scanning electron microscope was used to study the developed packaging structure. Antimicrobial activity of films against Listeria innoucua and L. monocytogenes was tested both in vitro and in bologna samples. Results showed that values of load at break decreased with increasing concentrations of pediocin at 10 °C and 25 °C. Regarding water vapor permeability, only the control and 50 % pediocin films presented statistical difference, with the 50 % pediocin film being more permeable. In vitro tests showed antimicrobial activity against L. innocua. Cellulosic film with 50 % pediocin reduced L. monocytogenes growth on sliced bologna by 1.2 log cycles after 9 days and prevented biofilm formation on packaging and bologna surfaces. Hence, active cellulosic films made with 50 % pediocin in the form of commercially available concentrated ALTA™ 2341 have the potential of being used in a system of hurdle technologies as a final obstacle for L. monocytogenes control in bologna preservation.

scholarly journals In Vitro Control of Uropathogenic Microorganisms with the Ethanolic Extract from the Leaves of Cochlospermum regium (Schrank) Pilger

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Danny Ellen Meireles Leme ◽  
Allan Belarmino Rodrigues ◽  
Adriana Araújo de Almeida-Apolonio ◽  
Fabiana Gomes da Silva Dantas ◽  
Melyssa Fernanda Norman Negri ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Genitourinary Infections

The roots of Cochlospermum regium, popularly known as “algodãozinho-do-cerrado,” are used for the treatment of genitourinary infections. However, the removal of their subterranean structures results in the death of the plant, and the use of the leaves becomes a viable alternative. Therefore, the antimicrobial activity of Cochlospermum regium leaf’s ethanolic extract and its action on the biofilm formation of microorganisms associated with urinary infection were evaluated. The total phenolic compounds, flavoids, and tannins were quantified using the reagents Folin-Ciocalteu, aluminum chloride, and vanillin, respectively. The antimicrobial activity was evaluated by the broth microdilution method and the effect of the extract in the biofilm treatment was measured by the drop plate method. Cytotoxicity was evaluated by the method based on the reduction of MTS and the mutagenicity by the Ames test. The ethanolic extract of C. regium leaves presented 87.4 mg/EQ of flavonoids, 167.2 mg/EAG of total phenolic compounds, and 21.7 mg/ECA of condensed tannins. It presented reduction of the biofilm formation for E. coli and C. tropicalis and antimicrobial action of 1 mg/mL and 0.5 mg/mL, respectively. The extract showed no cytotoxicity and mutagenicity at the concentrations tested. This study demonstrated that C. regium leaves are a viable option for the treatment of genitourinary infections and for the species preservation.

In Vitro Study of Streptococcus mutans Biofilm Formation on Vanillin-Incorporated Orthodontic PMMA Resin

2019 ◽  
Vol 801 ◽  
pp. 9-14 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antimicrobial Property ◽  
Cell Counting ◽  
Bacterial Suspension ◽  
Orthodontic Appliances ◽  
Increased Risk ◽  
Removable Orthodontic Appliances ◽  
Pmma Resin

Removable orthodontic appliances made of poly methyl methacrylate (PMMA) resin are commonly used after complete orthodontic treatment to keep teeth in the right position and prevent the shift out from the alignment. The use of these appliances can place patients at increased risk of dental caries through the biofilm accumulation ofStreptococcus mutans, caries-associated bacteria, on the surfaces of appliance themselves. Moreover, their rough surfaces or intrinsic porosities of PMMA enhance a condition for plaque aggregation, altering the oral microflora and limiting the washing out action of saliva on teeth and mucosal tissues, subsequently bringing about enamel demineralization and gingival inflammation. Cleaning by brushing or using chemical disinfectants is necessary for hygiene maintenance of the appliances, but requires patient cooperation, which is uncertain. Vanillin is a natural phenolic aldehyde which is used widely as a flavoring agent in various foods. It has been reported by many studies to exhibit antimicrobial activity. The aim of the present study was to evaluate antimicrobial property of vanillin-incorporated orthodontic PMMA resin onS. mutansbiofilm formation. The self-curing orthodontic PMMA resin samples (SCG Chemicals, Thailand) were prepared according to the percentage of vanillin incorporation (0%, 0.1% and 0.5% vanillin). Another group of commercial resin samples without vanillin (Dentsply Caulk, USA) was prepared in the same manner. All samples were coated with sterile unstimulated saliva collected from three healthy adult volunteers at 37 °C for 60 min. TheS. mutansbiofilm formation was done with the 107colony forming unit (CFU)/m of bacterial suspension in 96-well plate and incubated at 37°C in 5% CO2 atmosphere for 24 h. The amount of biofilm was quantified by Cell Counting Kit WST-8 (Dojindo Molecular Technologies, USA) at 450 nm. All tests were performed in triplicate on three separate occasions. One-way ANOVA and Turkey’s test were used for the statistical analysis. A significant inhibitory effect was observed in 0.5% vanillin incorporated samples at 48 h compared with 0% vanillin and commercial samples. The percentage of biofilm reduction was 30%. In conclusion, the incorporation of vanillin to self-curing orthodontic PMMA resin could significantly inhibit biofilm formation ofS. mutans. Using this PMMA resin, removable orthodontic appliances with antimicrobial property can be applied to prevent dental caries or tooth demineralization in orthodontic patients.

scholarly journals Synthesis of a Small Library of Nature-Inspired Xanthones and Study of Their Antimicrobial Activity

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2405 ◽  
Author(s):  
Diana I. S. P. Resende ◽  
Patrícia Pereira-Terra ◽  
Joana Moreira ◽  
Joana Freitas-Silva ◽  
Agostinho Lemos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
X Ray Diffraction ◽  
X Ray ◽  
Xanthone Derivatives ◽  
Potent Inhibitory Effect ◽  
Resistant Strains

A series of thirteen xanthones 3–15 was prepared based on substitutional (appendage) diversity reactions. The series was structurally characterized based on their spectral data and HRMS, and the structures of xanthone derivatives 1, 7, and 8 were determined by single-crystal X-ray diffraction. This series, along with an in-house series of aminated xanthones 16–33, was tested for in-vitro antimicrobial activity against seven bacterial (including two multidrug-resistant) strains and five fungal strains. 1-(Dibromomethyl)-3,4-dimethoxy-9H-xanthen-9-one (7) and 1-(dibromomethyl)-3,4,6-trimethoxy-9H-xanthen-9-one (8) exhibited antibacterial activity against all tested strains. In addition, 3,4-dihydroxy-1-methyl-9H-xanthen-9-one (3) revealed a potent inhibitory effect on the growth of dermatophyte clinical strains (T. rubrum FF5, M. canis FF1 and E. floccosum FF9), with a MIC of 16 µg/mL for all the tested strains. Compounds 3 and 26 showed a potent inhibitory effect on two C. albicans virulence factors: germ tube and biofilm formation.

Sign in / Sign up

Export Citation Format

Share Document