scholarly journals Study of the anti-infl ammatory activity of the Potentilla anserina L on burn wounds in mice

2021 ◽  
pp. 113-116
Author(s):  
E.E. Savelieva ◽  
◽  
O.F. Veselova ◽  
I.V. Gackih ◽  
V.A. Horgevski ◽  
...  
Keyword(s):  
White Male ◽  
Hair Follicles ◽  
Thermal Burn ◽  
Experimental Animals ◽  
Burn Wounds ◽  
Percolation Method ◽  
Potentilla Anserina ◽  
Entire Experiment ◽  
Hematoxylin And Eosin ◽  
Active Processes

The aim of the research. To investigate the anti-infl ammatory eff ect of Cinquefoil on a model of a thermal wound. Material and methods. A liquid extract in 70 % ethyl alcohol was obtained from dry crushed goose cinquefoil by the percolation method. Th e resulting extracts were evaporated under vacuum at a temperature of 50 °C. Th e dry residue was dissolved in water immediately before treatment at a dose of 250 mg / kg. Th e evaluation of anti-infl ammatory activity was carried out in an experiment on 20 white male mice. Th ermal skin burns were simulated in all animals. Th e burns were healed in an open way. During the entire experiment, the general condition of the experimental animals was assessed, body weight and temperature were determined, the appearance of wounds, appetite, and activity were assessed. For histological examination, a section of the skin was taken, including the burn zones and intact tissues. Paraffi n sections were stained with hematoxylin and eosin. Results. An analysis of the results of the experimental study showed that all experimental animals, under the infl uence of high temperatures, developed a third degree thermal burn of the skin, characterized by damage to the entire thickness of the skin with complete death of hair follicles, sweat and sebaceous glands. At the site of the burn, the skin was dense, motionless and insensitive to painful stimuli. Th e appearance of the wounds did not diff er between groups of animals. Comparative microscopy of the control and experimental groups showed that the depth of damage to the epidermis and dermis was more pronounced in the experimental group. In addition, in the same group, lymphocytic infi ltration of the dermis, hypodermis, the presence of a pronounced vascular reaction and edema of the dermis are more pronounced, which indicates a more pronounced infl ammatory. Conclusion. Th e analysis of the results obtained shows that in the treatment of burn wounds, the local application of the preparation Potentilla goose on the 4th day in the experiment does not lead to a pronounced anti-infl ammatory eff ect. However, the revealed lymphocytic infi ltration of the dermis and other histological parameters indicate more active processes in the tissues, which may subsequently lead to more accelerated wound cleansing and faster regeneration

Dermal Nanoemulsion Treatment Reduces Burn Wound Conversion and Improves Skin Healing in a Porcine Model of Thermal Burn Injury

2021 ◽  
Author(s):  
Vladislav A Dolgachev ◽  
Susan Ciotti ◽  
Emma Liechty ◽  
Benjamin Levi ◽  
Stewart C Wang ◽  
...  
Keyword(s):  
Burn Injury ◽  
Hair Follicles ◽  
Silver Sulfadiazine ◽  
Histological Evaluation ◽  
Full Thickness ◽  
Burn Wound ◽  
Thermal Burn ◽  
Burn Wounds ◽  
Partial Thickness ◽  
Mediators Of Inflammation

Abstract Objective Burn wound progression is an inflammation driven process where an initial partial-thickness thermal burn wound can evolve over time to a full-thickness injury. We have developed an oil-in-water nanoemulsion formulation (NB-201) containing benzalkonium chloride for use in burn wounds that is antimicrobial and potentially inhibits burn wound progression. We used a porcine burn injury model to evaluate the effect of topical nanoemulsion treatment on burn wound conversion and healing. Methods Anesthetized swine received thermal burn wounds using a 25cm 2 surface area copper bar heated to 80 oC. Three different concentrations of NB-201 (10%, 20%, or 40% nanoemulsion), silver sulfadiazine cream or saline were applied to burned skin immediately after injury and on days 1, 2, 4, 7, 10, 14, and 18 post-injury. Digital images and skin biopsies were taken at each dressing change. Skin biopsy samples were stained for histological evaluation and graded. Skin tissue samples were also assayed for mediators of inflammation. Results Dermal treatment with NB-201 diminished thermal burn wound conversion to a full-thickness injury as determined by both histological and visual evaluation. Comparison of epithelial restoration on day 21 showed that 77.8% of the nanoemulsion treated wounds had an epidermal injury score of 0 compared to 16.7% of the silver sulfadiazine treated burns (p=0.01). Silver sulfadiazine cream and saline treated wounds (controls) converted to full-thickness burns by day 4. Histological evaluation revealed reduced inflammation and evidence of skin injury in NB-201 treated sites compared to control wounds. The nanoemulsion treated wounds often healed with complete regrowth of epithelium and no loss of hair follicles (NB-201: 4.8±2.1, saline: 0±0, silver sulfadiazine: 0±0 hair follicles per 4mm biopsy section, p<0.05). Production of inflammatory mediators and sequestration of neutrophils were also inhibited by NB-201. Conclusions Topically applied NB-201 prevented the progression of a partial-thickness burn wound to full-thickness injury and was associated with a concurrent decrease in dermal inflammation.

scholarly journals Electron microscopic changes of the testis germinal epithelium after an experimental thermal trauma in the application of cryo-lyophilized xenograft skin substrate

2018 ◽  
Vol 24 (4) ◽  
pp. 53-57
Author(s):  
K.S. Volkov ◽  
S.Yu. Muha
Keyword(s):  
Burn Injury ◽  
White Male ◽  
Male Rats ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Large Area ◽  
Burn Wound ◽  
Thermal Burn ◽  
Experimental Animals ◽  
Thermal Trauma

Deep, large area of thermal trauma of the skin leads to significant morphofunctional changes in organs in the composition of the burned organism. However, condition of the central organ of the male reproductive system - testicle after burns and, especially, in the treatment of thermally damaged areas of the skin remain poorly understood. The purpose of this study was to establish an ultrastructural re-organization of testis germinal epithelium cells in the stages of toxemia and septicotoxemia following an experimental thermal trauma when using xenograft skin substrate. The studies were carried out on 35 sexually mature white male rats, which were divided into three groups: 1 - intact animals (5 rats), 2 - animals with burn injury (15 rats), 3 - animals with burn injury, for which were used xenograft skin substrate (15 rats). The burn was applied under ketamine anesthesia with copper plates, heated in boiling water, on 1820% shaved body surface of animals. Early necrectomy of the damaged areas of skin was carried out 1 day after the thermal burn. The formed wounds were covered with cryo-lyophilized xenograft skin substrate. Experimental animals were decapitated at 7, 14 and 21 days of the experiment (early, late toxemia and septicotoxemia). The material sampling for electron microscopy was carried out in accordance with a generally accepted methodology. It is established that in the stage of early toxemia (7 days of experiment) there are adaptive-compensatory changes and signs of destruction of spermatogenic cells of testis. In the stages of late toxemia and septicotoxemia (14 and 21 days of the trial), significant destructive changes occur in all components of the seminiferous tubules of the organ. Electron microscopically found that the closure of the burn wound after the early necrectomy of cryo-lyophilized xenograft skin substrate in severe experimental burn injury in the early stages of the experiment significantly reduces the damage of testis germinal epithelium of experimental animals and activates regenerative processes. This contributes to a significant improvement in the ultrastructure of the components of the convoluted tubules of the organ in the late stages of the experiment.

scholarly journals Initial Tests on Selected Serological Reactions in Rabbits Burnt with Napalm and Subjected to Hyperbaric Oxygenation

2015 ◽  
Vol 50 (1) ◽  
pp. 41-45
Author(s):  
Kazimierz Ulewicz ◽  
Przemysław Michniewski ◽  
Brunon Kierznikowicz ◽  
Bogdan Łokucijewski ◽  
Jan Stencel
Keyword(s):  
Hyperbaric Oxygenation ◽  
Healing Process ◽  
Control Group ◽  
Complement Activity ◽  
Experimental Animals ◽  
Burn Wounds ◽  
Immunological Reactions

Abstract Hyperbaric oxygenation therapy has found its application in the treatment of various types of burns. The presented work describes the use of this method in treating napalm burns in experimental animals. The researchers took particular interest in the immunological reactions occurring in animals, as well as the effect of the said therapy on the healing process. Two groups of rabbits, previously anaesthetised with Evipan and immunised with S. typhi phage F7 microorganisms, were burnt with the use of napalm. The tested group was subjected to a series of treatment sessions with 100% oxygen at the pressure of 2 atm, whereas the control group did not undergo such treatment. Six repeated tests on complement activity with 50% haemolysis method and heamagglutination reaction quantification with antigens O and H S.typhi phage F7 were carried out on all of the researched animals. Moreover, for the purpose of control of the healing process, a number of histopathological exams on the burn wounds were conducted in both groups of rabbits. The research showed an increase in complement activity in all tested animals; however, in the tested group it occurred later. Histopathological tests confirmed a more advanced healing process in the group subjected to hyperbaric oxygenation.

scholarly journals Immune histochemical study of KI-67 level and ribonucleic acid in the process of healing of burn wounds after treatment with drugs containing dexpanthenol and ceramide

2019 ◽  
Vol 9 (6) ◽  
pp. 4586-4590 ◽  
Keyword(s):  
Ribonucleic Acid ◽  
Healing Time ◽  
Ki 67 ◽  
Thermal Burn ◽  
Burn Wounds ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Regenerative Activity ◽  
Reference Preparation ◽  
Wound Healing Time

Ceramides are structural components of the stratum corneum that regulate cell proliferation and differentiation. The study of the effect of the cream containing dexpanthenol and ceramide on the proliferation processes and regeneration of burn wounds in rats. Experimental rats with III-A degree thermal burn were treated separately with the following preparations: cream with ceramides, cream with dexpanthenol and ceramides and cream "Bepanten". The proliferative-regenerative activity of the drugs was investigated by studying the content of the Ki-67 producing cells and the content of ribonucleic acid (RNA) in the cytoplasm of the granulation tissue fibroblasts. Ceramides contributed to wounds healing, increasing the content of Ki-67 antigenpositive cells, accelerating the formation of scar in the wound. The therapeutic effect of creams with ceramides exceeds the efficacy of the reference preparation “Bepanten” cream. The addition of ceramides to dexpanthenol increases the quality and reduces the wound healing time.

scholarly journals A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1153
Author(s):  
Verena Schneider ◽  
Daniel Kruse ◽  
Ives Bernardelli de Mattos ◽  
Saskia Zöphel ◽  
Kendra-Kathrin Tiltmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Healing Process ◽  
Three Dimensional ◽  
Source Model ◽  
Burn Wound ◽  
Thermal Burn ◽  
Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

scholarly journals Systematic Review of Stem-Cell-Based Therapy of Burn Wounds: Lessons Learned from Animal and Clinical Studies

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2545
Author(s):  
Josefine Lin Henriksen ◽  
Nana Brandborg Sørensen ◽  
Trine Fink ◽  
Vladimir Zachar ◽  
Simone Riis Porsborg
Keyword(s):  
Wound Healing ◽  
Systematic Reviews ◽  
Clinical Studies ◽  
Meta Analysis ◽  
Lessons Learned ◽  
Burn Patients ◽  
Thermal Burn ◽  
Burn Wounds ◽  
Cell Based Therapy ◽  
Healing Phase

Treatment of severe burn wounds presents a daunting medical challenge, and novel approaches promoting healing and reducing scarring are highly desirable. The application of mesenchymal stem/stromal cells (MSCs) has been suggested as a novel treatment. In this paper, we present systematic reviews of pre-clinical and clinical studies of MSC therapy for second- or third-degree thermal burn wounds. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, the PubMed and Embase databases were searched, and interventional studies of MSC therapy using rodent models (21 studies) or human burn patients (three studies) were included in the pre-clinical and clinical reviews, respectively, where both overall outcome and wound-healing-phase-specific methodologies and effects were assessed. The pre-clinical studies demonstrated a promising effect of the application of MSCs on several wound healing phases. The clinical studies also suggested that the MSC treatment was beneficial, particularly in the remodeling phase. However, the limited number of studies, their lack of homogeneity in study design, relatively high risk of bias, lack of reporting on mode of action (MOA), and discontinuity of evidence restrict the strength of these findings. This comprehensive review presents an overview of available methodologies to assess the MOA of MSC treatment for distinct wound healing phases. Furthermore, it includes a set of recommendations for the design of high-quality clinical studies that can determine the efficacy of MSCs as a therapy for burn wounds.

scholarly journals The influence of multiphytoadaptogen on leukocyte infiltration in mice hepatocarcinomas

2019 ◽  
Vol 18 (2) ◽  
pp. 60-65
Author(s):  
R. V. Karpova ◽  
E. V. Bocharov ◽  
O. A. Bocharova ◽  
I. V. Kazeev ◽  
V. G. Kucheryanu ◽  
...  
Keyword(s):  
Leukocyte Infiltration ◽  
Male Mice ◽  
Liquid Form ◽  
Liver Tumours ◽  
Tissue Samples ◽  
Experimental Animals ◽  
Tumour Formation ◽  
Hematoxylin And Eosin ◽  
Mice Liver ◽  
Therapeutic Administration

The aim of this study was the morphological investigation of the CBA mice liver tissue at different stages of ontogenesis as well as during liquid form multifitoadaptogen therapeutic administration.Materials and methods . The study objects are the liver samples of CBA male mice (subline CBA / LacY). Experimental animals received a 10 % solution of multifitoadaptogen from 6 months of age until natural death. Tissue samples were fixed in neutral buffered formalin and embedded in paraffin. Sections from paraffin blocks were stained with hematoxylin and eosin. The structure of the liver tumours was determined as well as the quantitаtive degree of leukocyte infiltration in the tumour tissue.Results . The tumours morphologically structured as moderately differentiated trabecular hepatocarcinomas (at 8 months of age) and low differentiated trabecular-acinar hepatocarcinomas (aged 22 months) were revealed in CBA male mice. Higher lever of leukocyte infiltration in hepatocarcinomas of experimental animals was determined.Conclusion . Leukocyte infiltration may be important for antitumour immune reaction as well as for reduction of the tumour formation incidence in high-cancer mice.

scholarly journals Features of morphological changes in the thyroid gland of white male rats 1 day after thermal trauma of the skin on the background of the introduction of 0.9 % NaCl solution

2019 ◽  
pp. 55-59
Author(s):  
O. I. Tiron
Keyword(s):  
Thyroid Gland ◽  
White Male ◽  
Experimental Studies ◽  
Vena Cava ◽  
Scientific Data ◽  
The Body ◽  
Male Rats ◽  
Nacl Solution ◽  
Experimental Animals ◽  
Thermal Trauma

Despite the fact that the main pathogenetic factor in the development of burn disease is the alteration of the skin, the severity of the condition of patients in the acute period of the disease is due to systemic changes in the body caused by thermal factor. Being a powerful stress factor, severe burns are accompanied by activation of the hypothalamic-pituitary system. Although thyroid hormones are not considered typical "stress hormones", they also play a role in maintaining vasomotor function under stress. The question of thyroid dysfunction on the background of burn injury remains debatable, and scientific data on its morphological and ultramicroscopic changes are quite limited. The aim of the work is to establish microscopic and submicroscopic changes of the thyroid gland of experimental animals in the stage of shock after thermal trauma of the skin. Experimental studies were performed on 12 white adult male rats, which received a thermal burn of the skin of 2-3 degrees with a lesion area of 21-23 % of the skin. An infusion of 0.9 % NaCl solution at a dose of 10 ml/kg was performed into the inferior vena cava. Collection of material for histological and electron microscopic examinations was performed according to generally accepted methods. Histological specimens were examined using a MIKROmed SEO SCAN light microscope and photo-documented using a Vision CCD Camera with an image output system from histological specimens. Ultrathin sections made on an ultramicrotome UMPT-7 were contrasted with uranyl acetate, lead citrate according to the Reynolds method and studied under an electron microscope PEM-125K. Conducted micro- and submicroscopic studies of the structural components of the thyroid gland a day after the reproduction of thermal trauma to the skin on the background of the introduction of 0.9 % NaCl solution revealed reactive adjunctive-compensatory changes and initial manifestations of destruction. Vascular, stromal and parenchymal components of the organ in the stage of shock after burns correspond to the state of "stress" as a reaction to pathological exogenous exposure, and the intensification of metabolic processes in the affected organism of experimental animals.

scholarly journals Biological evaluation of the influence of protein hydrolysate on the organism of the monks

2019 ◽  
Vol 1 (1) ◽  
pp. 52-54
Author(s):  
N. A. Slesarenko ◽  
P. N. Abramov
Keyword(s):  
Amino Acid ◽  
Test Group ◽  
Biological Evaluation ◽  
Hair Follicles ◽  
Control Group ◽  
Skin Thickness ◽  
Local Effects ◽  
Experimental Animals ◽  
Protein Hydrolyzate ◽  
Basic Diet

In this study, performed on the basis of JSC "Plemzverosovkhoz Saltykovsky" on 150 standard minks which were selected according to the principle of analogs, the systemic and local effects of protein hydrolyzate in the composition of the basic diet were evaluated. A complex methodical approach was used, including clinical, hematological, morphological and zootechnical research methods. The digital material was subject o statistical processing. When analyzing the basic diet of minks, it was established that it is balanced in almost all nutritional substances. Nevertheless, based on a comparative study of the amino acid composition of the feed, a deficiency of the essential amino acid of threonine was detected. An evaluation of the dynamics of body weight in experimental animals showed an advantage in the growth of animals receiving hydrolyzate. By the end of the experiment, the animals of the experimental groups outstripped (by 10.1%) the control analogues for weight gain. The results of the analysis of biochemical parameters of blood serum testify to a statistically significant increase in the concentration of the total protein (by 48.6% in test group minks relative to the control group) with a simultaneous decrease (by 27%) of the albumin fraction. The level of ALT in the control group was lower (by 29%) than in the animals of the experimental groups, while increasing (by 35%) the level of AST. Morphometric parameters of the total skin thickness in experimental animals did not have significant differences. At the same time, their ratio in the general cover varies in the direction of diminishing the thickness of the epidermis and the depth of the hair follicles in animals treated with hydrolyzate. Planimetric indicators of skins raw materials in experienced animals increased by 5.5% compared to representatives of the control group. Based on the evaluation of systemic and local effects of protein hydrolyzate, the effectiveness of its use in cellular fur farming has been established.

Nonshivering thermogenesis induced by repetitive cooling of spinal cord in the rat

1976 ◽  
Vol 230 (3) ◽  
pp. 720-723 ◽  
Author(s):  
M Banet ◽  
H Hensel
Keyword(s):  
Spinal Cord ◽  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Rectal Temperature ◽  
Cold Adaptation ◽  
White Male ◽  
Metabolic Effect ◽  
Male Rat ◽  
Nonshivering Thermogenesis ◽  
Experimental Animals

The effect of prolonged and repetitive cooling of the spinal cord on the sensitivity to the metabolic effect of exogenous noradrenaline and on the resistance to cold exposure was studied in the white male rat. The spinal cord of 10 animals was cooled for an average of 90 h-9 h/day 5 days/wk - to a level that induced an increase in oxygen uptake of almost 70%. Oxygen consumption was then measured at 30 degrees C before and 1 h after a subcutaneous injection of noradrenaline (0.4 mg/kg). Following the noradrenaline injection, the experimental animals increased oxygen uptake by 71%, while the control ones increased it by only 33% (P less than 0.01). During exposure to -20 degrees C, the experimental animals, despite their increased capacity for nonshivering thermogenesis, did not maintain rectal temperature longer than the control ones, thus showing that other factors also play a significant role in cold adaptation in the rat.

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