scholarly journals Immune histochemical study of KI-67 level and ribonucleic acid in the process of healing of burn wounds after treatment with drugs containing dexpanthenol and ceramide

2019 ◽  
Vol 9 (6) ◽  
pp. 4586-4590 ◽  
Keyword(s):  
Ribonucleic Acid ◽  
Healing Time ◽  
Ki 67 ◽  
Thermal Burn ◽  
Burn Wounds ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Regenerative Activity ◽  
Reference Preparation ◽  
Wound Healing Time

Ceramides are structural components of the stratum corneum that regulate cell proliferation and differentiation. The study of the effect of the cream containing dexpanthenol and ceramide on the proliferation processes and regeneration of burn wounds in rats. Experimental rats with III-A degree thermal burn were treated separately with the following preparations: cream with ceramides, cream with dexpanthenol and ceramides and cream "Bepanten". The proliferative-regenerative activity of the drugs was investigated by studying the content of the Ki-67 producing cells and the content of ribonucleic acid (RNA) in the cytoplasm of the granulation tissue fibroblasts. Ceramides contributed to wounds healing, increasing the content of Ki-67 antigenpositive cells, accelerating the formation of scar in the wound. The therapeutic effect of creams with ceramides exceeds the efficacy of the reference preparation “Bepanten” cream. The addition of ceramides to dexpanthenol increases the quality and reduces the wound healing time.

scholarly journals Constructing a Sr2+-Substituted Surface Hydroxyapatite Hexagon-Like Microarray on 3D-Plotted Hydroxyapatite Scaffold to Regulate Osteogenic Differentiation

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1672
Author(s):  
Yingqi Wei ◽  
Huichang Gao ◽  
Lijing Hao ◽  
Xuetao Shi ◽  
Yingjun Wang
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Hydrothermal Reaction ◽  
Porous Scaffold ◽  
Healing Time ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Hydroxyapatite Scaffold ◽  
Biological Performance ◽  
Strontium Ion

Surface topography and chemical characteristics can regulate stem cell proliferation and differentiation, and decrease the bone-healing time. However, the synergetic function of the surface structure and chemical cues in bone-regeneration repair was rarely studied. Herein, a strontium ion (Sr2+)-substituted surface hydroxyapatite (HA) hexagon-like microarray was successfully constructed on 3D-plotted HA porous scaffold through hydrothermal reaction to generate topography and chemical dual cues. The crystal phase of the Sr2+-substituted surface microarray was HA, while the lattice constant of the Sr2+-substituted microarray increased with increasing Sr2+-substituted amount. Sr2+-substituted microarray could achieve the sustainable release of Sr2+, which could effectively promote osteogenic differentiation of human adipose-derived stem cells (ADSCs) even without osteogenic-induced media. Osteogenic characteristics were optimally enhanced using the higher Sr2+-substituted surface microarray (8Sr-HA). Sr2+-substituted microarray on the scaffold surface could future improve the osteogenic performance of HA porous scaffold. These results indicated that the Sr2+-substituted HA surface hexagon-like microarray on 3D-plotted HA scaffolds had promising biological performance for bone-regeneration repair scaffold.

scholarly journals Healing the third degree thermal burn in mice by Dielectric Barrier Discharge Plasma (DBD plasma): Comparison and evaluation of area, burn wound healing time, burn skin temperature

10.29007/fmkc ◽  
2020 ◽  
Author(s):  
Thi Minh Hien Ngo ◽  
Tuyet Nhi Do ◽  
Quoc Duy Nam Nguyen ◽  
Duy Phuong Nguyen ◽  
Nguyen Ngan Ha Lam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Time ◽  
Diagnostic Methods ◽  
Invasive Treatment ◽  
Burn Wound ◽  
Dbd Plasma ◽  
Thermal Burn ◽  
Non Invasive ◽  
Burn Wound Healing ◽  
Wound Healing Time

Burns are one of the most devastating conditions encountered in medicine. This injury is in skin or other tissues, caused by heat-cold, electricity or chemicals [1,2]. There are lots of methods to treat burns and each method has its own advantages, such as medicine, dressing, low-level laser, plasma, skin graft surgery... [3].This study experimented with the 3rd degree burn model in mice by heat, treating by DBD plasma, is a non-invasive treatment and using clinical diagnostic methods by (1) normal image, (2) thermal image, (3) HE staining. Aim of this research is evaluation and comparison the area, temperature and wound healing time of non-invasive treatment with DBD plasma and nontreatment. After 3-week experiment, using diagnostic methods and analysis tools have demonstrated that the 3rd thermal burn wound healing of plasma treatment recovers faster than non-treatment about: (1) Burn wound surface shrinkage rate is higher: ~ 5%; (2) Healing time is faster: 2-3 days; (3) The average temperature of the burn wound is lower: 1-2oC. Therefore, DBD plasma is a potential treatment in burns wound and wound healing in the future. Keywords: DBD plasma, burn wound, healing wound

Effects and Safety of Different Silver Preparation in Burns Treatment: A Bayesian Network Meta-analysis

2021 ◽  
pp. 153473462110040
Author(s):  
Dilixiati Kuerban ◽  
Maisiwuti Maimaiti ◽  
Zhao Chen
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Statistical Difference ◽  
Meta Analysis ◽  
Healing Time ◽  
Cochrane Library ◽  
Burn Wounds ◽  
Randomized Controlled ◽  
The Cochrane Library ◽  
Wound Healing Time

Silver formulation has been used for external use of burn wounds for several decades, mainly including silver sulfadiazine (SSD), nanosilver dressing (NSD), and silver ion dressing (SID). At present, there is no simultaneous comparison of the effects of silver formulation on burn wounds. The databases were retrieved in an orderly manner from the dates of their establishment to May 2020, including PubMed, the Cochrane Library, Web of Science, and Clinical Trials. Then a network meta-analysis was conducted using R and RevMan 5.1 software. A total of 13 randomized controlled trials (RCTs) involving 945 patients with burns were included. A pairwise meta-analysis of the results was presented: the wound healing time in the SID or NSD treatment group was less than that in the SSD group; and in relieving the pain there was a statistical difference between the SSD, SID, or NSD groups. Network meta-analysis of the results was presented: the wound healing time and relieving the pain in the SID or NSD treatment group were less than that in the SSD group, but there was no statistical difference between the SID and NSD groups. The possibility of NSD in the wound healing time being the best treatment was 75.2%, followed by SID (36.6%), and finally SSD (1.1%); and the possibility of NSD being the best relieving the pain was 83.5%; followed by SID (60.0%), and finally SSD (16.3%). According to the evidence, treatment for burns with NSD can improve the wound healing time and relieve the pain of wounds.

scholarly journals The results of experimental-clinical study of healing of burn wounds and prevention of their complications under the influence of nano-containing biodegradable polymer technologies

2020 ◽  
Vol 19 (1) ◽  
pp. 32-36
Author(s):  
O. Ya. Popadyuk ◽  
E. O. Kindrativ ◽  
K. O. Kirshak ◽  
A. M. Yavorsky
Keyword(s):  
Wound Healing ◽  
Polymer Film ◽  
Biodegradable Polymer ◽  
Initial Treatment ◽  
Secondary Infection ◽  
Animal Experiments ◽  
Healing Time ◽  
Burn Wounds ◽  
Group I ◽  
Wound Healing Time

Burns cause high rates of morbidity and mortality worldwide. Improper initial treatment or delayed initial treatment can have adverse consequences for further wound healing. Goal. To study the effect of biodegradable polymer films «biodep-nano» on the healing of burns of the skin and prevention of complications. The aim of the study was to investigate the effect of our developed biodegradable polymer film «biodep-nano», which is additionally saturated with hydrated fullerene C60 on the healing of severe suppurative burns in animal experiments and the effectiveness of this film in the prevention of secondary infection in patients with mild burns. Material and methods. Simulation of skin burns was performed on 50 adult white Wistar rats weighing an average of 300.0 g, St.Aureus was injected under the scab, after 3 days the scab was removed surgically and the wound was cleaned with a biodep-nano film, the material was replaced every 2 days. with planimetric, microbiological and pathomorphological examination on the 3rd, 7th, 14th and 21st day. In the conditions of the burn department of Ivano-Frankivsk OKL, the biodegradable polymer film “biodep nano” introduced into production was used to prevent secondary infection of burn wounds. Results. When using the film developed by us, the area decreased by 21.6 % on the 3rd day, by 44.5 % on the 7th day, by 67.8 % on the 14th day. By 21 days there was a complete closure of the wound defect. The results of the microbiological study indicate that the level of contamination of burn wounds after application of the studied film of zinc nanooxide decreased during treatment and on the 3rd day to (4.3±3.3)*105 CFU/ml, which is the level of bacteria that is not pathogenic. After the 14th day, the pathogen was not sown and the wounds healed well. The obtained data showed that in group I where the film was used, the secondary infection was 11 % lower, which in turn showed a shorter wound healing time, which was 2 days less than in group II. Conclusions. The biodegradable material developed by us reliably protects burn wounds from accidental infection, is safetyless, elastic, and in experimental research demonstrated high medical properties in relation to the purulent burn wounds of IIIb century.

Role of melatonin in regulating neurogenesis: Implications for the neurodegenerative pathology and analogous therapeutics for Alzheimer’s disease

2020 ◽  
Vol 3 (2) ◽  
pp. 216-242 ◽  
Author(s):  
Mayuri Shukla ◽  
Areechun Sotthibundhu ◽  
Piyarat Govitrapong
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Adult Neurogenesis ◽  
Adult Brain ◽  
Therapeutic Approaches ◽  
Therapeutic Implications ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Progenitor Cell Proliferation

The revelation of adult brain exhibiting neurogenesis has established that the brain possesses great plasticity and that neurons could be spawned in the neurogenic zones where hippocampal adult neurogenesis attributes to learning and memory processes. With strong implications in brain functional homeostasis, aging and cognition, various aspects of adult neurogenesis reveal exuberant mechanistic associations thereby further aiding in facilitating the therapeutic approaches regarding the development of neurodegenerative processes in Alzheimer’s Disease (AD). Impaired neurogenesis has been significantly evident in AD with compromised hippocampal function and cognitive deficits. Melatonin the pineal indolamine augments neurogenesis and has been linked to AD development as its levels are compromised with disease progression. Here, in this review, we discuss and appraise the mechanisms via which melatonin regulates neurogenesis in pathophysiological conditions which would unravel the molecular basis in such conditions and its role in endogenous brain repair. Also, its components as key regulators of neural stem and progenitor cell proliferation and differentiation in the embryonic and adult brain would aid in accentuating the therapeutic implications of this indoleamine in line of prevention and treatment of AD.   

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

The role of the oral cavity immune cells in the postoperative complications after face lifting

2018 ◽  
pp. 174-178
Author(s):  
Ш.М. Абрамян ◽  
Е.В. Рожнова ◽  
Е.Н. Волкова ◽  
С.Н. Блохин ◽  
С.Г. Морозов
Keyword(s):  
Oral Cavity ◽  
Immune Cells ◽  
Postoperative Period ◽  
Negative Impact ◽  
Tooth Enamel ◽  
Healing Time ◽  
Negative Contribution ◽  
Dental Diseases ◽  
Induced Apoptosis ◽  
Wound Healing Time

Цель. Изучение клеток иммунной системы полости рта у пациентов до проведения операции лифтинга лица, а также сопоставление этих данных с показателями, выявленными при осложнениях в послеоперационном периоде. Методика. Исследованы клетки иммунной системы полости рта 100 женщин (23-68 лет), которым перед операцией лифтинга лица проведено стоматологическое обследование и необходимое лечение при наличии кариеса дентина, клиновидного дефекта эмали зуба, хронического периодонтита или пульпита. Выделенные путем смывов десенной борозды клетки окрашивали моноклональными антителами и анализировали на проточном цитометре. Определяли уровень спонтанного и индуцированного апоптоза. Оценивали уровень фагоцитоза и генерацию супероксидного аниона нейтрофилами. Результаты. Показано, что хронические воспалительные заболевания зубов оказывают негативное влияние на состояние иммунных клеток ротовой полости, сопровождаются повышением генерации супероксидного аниона нейтрофилами, повышением уровня спонтанного и церамид-индуцированного апоптоза клеток десенной борозды. Заключение. Наличие хронической патологии зубов, даже после санации, оказывает негативное влияние на течение послеоперационного периода при проведении операции лифтинга лица, в частности, способствует увеличению времени заживления операционной раны, инфицированию раны с появлением очагов некроза в области операционного шва. The object. The study of the cells of the immune system of the oral cavity in patients before the operation of face lifting, as well as a comparison of these indicators with complications in the postoperative period. Methods. The immune cells from the oral cavity were studied in 100 women (23-68 years), who underwent a dental examination and necessary treatment if they had the dentin caries, wedge-shaped defect of tooth enamel, chronic periodontitis or pulpitis before the facial lifting operation. The immune cells have been isolated by a lavage of gingival sulcus around the damaged tooth. Results. It has been shown that chronic dental diseases made a negative contribution to the oral cavity immune cells. It has been accompanied by the elevated levels of superoxide originating from neutrophils as well as the increased levels of spontaneous and ceramide-induced apoptosis of immune cells isolated from the gingival sulcus. Conclusion. The presence of chronic pathology of teeth even in the case of the preoperative dental sanation has a negative impact on the postoperative period after face lifting, in particular, contributes to the lengthening of the surgical wound healing time, wound infection as well as the partial necrosis of suture.

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

MicroRNA Determines the Fate of Intestinal Epithelial Cell Differentiation and Regulates Intestinal Diseases

2019 ◽  
Vol 20 (7) ◽  
pp. 666-673 ◽  
Author(s):  
Sujuan Ding ◽  
Gang Liu ◽  
Hongmei Jiang ◽  
Jun Fang
Keyword(s):  
Target Genes ◽  
Gastrointestinal Disease ◽  
Intestinal Barrier ◽  
Vital Role ◽  
Intestinal Barrier Function ◽  
Intestinal Epithelial ◽  
Intestinal Function ◽  
Cell Fates ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

The rapid self-renewal of intestinal epithelial cells enhances intestinal function, promotes the nutritional needs of animals and strengthens intestinal barrier function to resist the invasion of foreign pathogens. MicroRNAs (miRNAs) are a class of short-chain, non-coding RNAs that regulate stem cell proliferation and differentiation by down-regulating hundreds of conserved target genes after transcription via seed pairing to the 3&#039; untranslated regions. Numerous studies have shown that miRNAs can improve intestinal function by participating in the proliferation and differentiation of different cell populations in the intestine. In addition, miRNAs also contribute to disease regulation and therefore not only play a vital role in the gastrointestinal disease management but also act as blood or tissue biomarkers of disease. As changes to the levels of miRNAs can change cell fates, miRNA-mediated gene regulation can be used to update therapeutic strategies and approaches to disease treatment.

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