scholarly journals Biological evaluation of the influence of protein hydrolysate on the organism of the monks

2019 ◽  
Vol 1 (1) ◽  
pp. 52-54
Author(s):  
N. A. Slesarenko ◽  
P. N. Abramov
Keyword(s):  
Amino Acid ◽  
Test Group ◽  
Biological Evaluation ◽  
Hair Follicles ◽  
Control Group ◽  
Skin Thickness ◽  
Local Effects ◽  
Experimental Animals ◽  
Protein Hydrolyzate ◽  
Basic Diet

In this study, performed on the basis of JSC "Plemzverosovkhoz Saltykovsky" on 150 standard minks which were selected according to the principle of analogs, the systemic and local effects of protein hydrolyzate in the composition of the basic diet were evaluated. A complex methodical approach was used, including clinical, hematological, morphological and zootechnical research methods. The digital material was subject o statistical processing. When analyzing the basic diet of minks, it was established that it is balanced in almost all nutritional substances. Nevertheless, based on a comparative study of the amino acid composition of the feed, a deficiency of the essential amino acid of threonine was detected. An evaluation of the dynamics of body weight in experimental animals showed an advantage in the growth of animals receiving hydrolyzate. By the end of the experiment, the animals of the experimental groups outstripped (by 10.1%) the control analogues for weight gain. The results of the analysis of biochemical parameters of blood serum testify to a statistically significant increase in the concentration of the total protein (by 48.6% in test group minks relative to the control group) with a simultaneous decrease (by 27%) of the albumin fraction. The level of ALT in the control group was lower (by 29%) than in the animals of the experimental groups, while increasing (by 35%) the level of AST. Morphometric parameters of the total skin thickness in experimental animals did not have significant differences. At the same time, their ratio in the general cover varies in the direction of diminishing the thickness of the epidermis and the depth of the hair follicles in animals treated with hydrolyzate. Planimetric indicators of skins raw materials in experienced animals increased by 5.5% compared to representatives of the control group. Based on the evaluation of systemic and local effects of protein hydrolyzate, the effectiveness of its use in cellular fur farming has been established.

ZINC IN THE RATIONS OF CALVES

2019 ◽  
Keyword(s):  
Body Mass ◽  
Test Group ◽  
Hair Follicles ◽  
Control Group ◽  
Albumin Concentration ◽  
Zinc Compounds ◽  
Entire Duration ◽  
Zinc Salts ◽  
Group A ◽  
Hair Cortex

According to the Tambovagrochemcenter data, zinc deficiency in animal rations exceeds 33%. To de-termine the efficiency of ration additives 3 groups of calves were formed, one of which was control and received regular feed, first test group received additional Zinc Sulphate, and second test group received additional Zinc Bioplex. To assess the results, we take into account the following values: chemical com-position and nutritional values of the feed, body mass value, amount of immunoglobulins in blood, bio-chemical and hematological values of blood, morphological values of fur and skin. Noticeable results were achieved in 4 months, with first and second test groups receiving accordingly 3,15% and 6,13% more body mass increase over the control group. A tendency towards higher albumin concentration in both test groups was noted, and calves of the second test group had higher amounts of hemoglobin and higher concentrations of it per erythrocyte. In samples of epidermis and hairs of the second test group the papillary layer was well developed, with the amount of hair and hair follicles exceeding 7 in the field of view, with the thickness of hairs being at least 70 μm. Inner papilla of hair contained 6 to 8 layers of hair, thickness of awn hairs reaching 80-90 μm, down hairs reaching 40 μm, with the hair cortex making up more than 80% of the hair thickness. Thus, introducing zinc salts and chelated zinc compounds to the milk-fed calves’ ration, has resulted in monetary profit increases over the control group by 255,0 and 612,0 roubles accordingly. By all researched values, the highest results were reached from adding zinc bioplex with approximately 360 mg added per animal for the entire duration of the growth process.

scholarly journals PHYSIOLOGICAL STATUS AND PRODUCTIVITY OF TURKEY-BROILERS WHEN USE FEED SUPPLEMENTS IN THEIR RATIONS

2017 ◽  
pp. 5-9
Author(s):  
Айметов ◽  
Ruslan Aymetov
Keyword(s):  
Test Group ◽  
Mineral Elements ◽  
The Body ◽  
Control Group ◽  
Physiological Status ◽  
Maximum Output ◽  
Mineral Supplement ◽  
Basic Diet ◽  
The Republic ◽  
Experimental Group

The mineral elements interact not only among themselves, but also with the organic components during complex metabolism process. Identifying the features of the feed nutrient substances relationship allows you to direct the body’s metabolism in the direction of their effectiveness and to obtain the maximum output from the animals. The experimental part of the work was carried out on poultry complex “Agrofirma Zalesnyy” of the Republic of Tatarstan from January to May 2016. The objects of research were female turkey-broilers of “Hybrid Converter” cross, mineral supplement “Tseostimul”, probiotic “Provagen” and symbiotic preparation of a new generation. With this aim, four groups of turkey were formed of 40 heads. The first group was a control one and received a basic diet, adopted by the poultry farm. The second group received the basic diet, added with mineral supplements with a dosage of 30 gramm per 1 kilogramm of feed. Turkeys of III-experimental group was added to the diet of probiotic preparation “Provagen” at a dose of 3 gramm per 1 kilogramm of feed. The turkeys of IV-test group received in addition to the basic diet a symbiotic drug rate of 20 gramm per 1 kilogramm of feed. The main diet was consisted of complete feed. Duration of the experiment was 112 days. By the end of the experiment the body weight of experimental groups exceeded the similar index: in the control group to 1082 gramm (р ≥0,001), in the first test group to 781 gramm (р ≥0,001), in the second test group to 1339 gramm (р ≥0,001), in the third group with addition of symbiotic drugs or to 12.3%, 8.9% and 15.3% respectively. The numbers of leukocytes was decreased from 34.2 109/L to 33.3 109/L in I experimental group, to 33.2 109/L in II and to 34.4 109/L in IV experimental group. The mass of internal organs was significantly higher in females of experimental groups. Thus, the weight of the liver of experimental groups was greater to 12.3%, 8.9% and 15.3%, compared with the control group.

scholarly journals Reversal of Alopecia Areata, Osteoporosis follow treatment with Activation of Tgr5 in mice

2021 ◽  
Author(s):  
Xiaohui Zhou ◽  
Zhiqiang Guan ◽  
Xiao Jin ◽  
Jianbin Zhao ◽  
Guisheng Chen ◽  
...  
Keyword(s):  
Alopecia Areata ◽  
Lumbar Vertebrae ◽  
Inflammatory Cells ◽  
Cell Number ◽  
Hair Follicles ◽  
Control Group ◽  
Skin Thickness ◽  
Wild Type ◽  
Control Group Design ◽  
Stat3 Signaling Pathway

Alopecia areata is an autoimmune hair loss disease with infiltration of pro-inflammatory cells into hair follicles. The role of Tgr5 in dermatitis has attracted considerable attention. This study aimed to investigate the effect of Tgr5 in the development of Alopecia areata. The study utilized a comparison control group design with four groups of wild type group, wild type+INT777 group, Tgr5-/- group, Tgr5-/-+INT777 group. The mice were treated with INT777(30mg/kg/day) or the carrier solution (DMSO) intraperitoneally for 7 weeks and the back skin was collected and analyzed by histology and immunohistochemistry staining. The lumbar vertebrae 4 has also been analyzed by DXA and Micro-CT. Tgr5-/- mice displayed the decreasingly significant in hair area and length, skin thickness, and the ratio of anagen and telogen, collagen, and mast cell number and loss the bone mass than WT group. After treating with INT777, the appearance of alopecia areata and bone microstructure has improved. Immunohistochemistry and qPCR analysis showed that activation of Tgr5 can down-regulate the express of JAK1, STAT3, IL-6, TNF-α, and VEGF.These findings indicate that activation of Tgr5 mediated amelioration of alopecia areata and osteoporosis by down-regulated JAK1-STAT3 signaling pathway.

scholarly journals Evaluation of efficacy of the amino acid-peptide complex administered intragastrically to golden hamsters experimentally infected with SARS-CoV-2

2021 ◽  
Author(s):  
DS Laptev ◽  
GA Protasova ◽  
SG Petunov ◽  
AS Radilov ◽  
SV Chepur ◽  
...  
Keyword(s):  
Amino Acid ◽  
Test Group ◽  
Positive Control ◽  
Lung Damage ◽  
Control Group ◽  
Lung Weight ◽  
Syrian Hamsters ◽  
Peptide Complex ◽  
Amino Acid Peptide ◽  
Treatment And Prevention

The development of coronavirus infection outbreak into a pandemic, coupled with the lack of effective COVID-19 therapies, is a challenge for the entire pharmaceutical industry. This study aimed to assess the treatment and preventive efficacy of the amino acid-peptide complex (APC) in male Syrian hamsters infected with SARSCoV-2 (intranasal administration of 26 μl of the virus culture, titer of 4 × 104 TCD50/ml). In a modeled COVID-19 case, APC administered for treatment and preventive purposes reduced lung damage. Compared to the positive control group, test group had the lung weight factor 15.2% smaller (trend), which indicates a less pronounced edema. Microscopic examination revealed no alveolar edema, atypical hypertrophied forms of type II alveolocytes, pulmonary parenchyma fibrinization. The macrophage reaction intensified, which is probably a result of the APC-induced activation of regenerative processes in the lung tissues. Spleens of the animals that received APC for therapeutic and preventive purposes were less engorged and had fewer hemorrhages. The decrease of body weight of the test animals that received APC for treatment and prevention was insignificant (p < 0.05), which indicates a less severe course of COVID-19. Administered following a purely therapeutic protocol, APC proved ineffective against SARS-CoV-2 post-infection. Thus, APC-based drug used as a therapeutic and preventive agent reduces pulmonary edema and makes morphological signs of lung tissue damage less pronounced in male Syrian hamsters infected with SARS-CoV-2.

SORBENTS AND PROBIOTICS IN FEEDING OF WEANLING PIGLETS

2016 ◽  
Vol 1 (3) ◽  
pp. 37-40
Author(s):  
Псхациева ◽  
Zemfira Pskhatsieva
Keyword(s):  
Body Weight ◽  
Average Daily Gain ◽  
Test Group ◽  
Live Weight ◽  
The Body ◽  
Control Group ◽  
Basic Diet ◽  
Weanling Piglets ◽  
Feed Costs ◽  
Experimental Group

The purpose of research is increase in economically useful qualities of piglets weaned with co-feeding of the sorbent Kovelos-Sorb and probiotic Sporotermin. Weanling piglets produced in 2 months of age. Duration of the experiment was 60 days, on reaching the age of 120 days. The first control group received a basic diet, the second experimental group – the basic diet and probiotic Sporotermin in the amount of 0.1% by weight of the feed, the third experimental group – the basic diet and sorbent Kovelos-Sorb in the amount of 0.1% by weight of the feed, the fourth test group – the basic diet, Sporotermin 0.1 by weight of the feed and Kovelos-Sorb 0.1% by weight of the feed. The studies found that the body weight of piglets at the age of 120 days in the second, third and fourth groups were higher by 4.1; 6.6 and 10.9%, respectively, relative to the first live weight of pigs group. It has also been found to increase average daily gain of 7.1; 11.7 and 19.4%, respectively, relative growth of the first group of piglets. Feed costs decreased by 3.7-16.3% in the experimental group, fed by the sorbent and the probiotic and probiotic sorbent together. With increasing body weight, the increase and feed decrease, there was an increase of beef entering the 0.4-1.6% relative to slaughter pigs release of the first group. Based on these data, we recommend the combined using of probiotic Sporoderm and sorbent Cavelos-Sorb, respectively, in the amount of 0.1% by weight of the feed for piglets-weaned at 2 months of age.

A Randomized Clinical Trial with a 0.6% Amino Acid/1.4% Glycerol Peritoneal Dialysis Solution

2004 ◽  
Vol 24 (3) ◽  
pp. 222-230 ◽  
Author(s):  
Wim Van Biesen ◽  
Walter Boer ◽  
Bart De Greve ◽  
Clement Dequidt ◽  
Denise Vijt ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Peritoneal Dialysis ◽  
Amino Acid ◽  
Randomized Clinical Trial ◽  
Glucose Solution ◽  
Test Group ◽  
Test Solution ◽  
Control Group ◽  
Glycerol Solution ◽  
Dialysis Solution

Background Glucose is an accepted osmotic agent for peritoneal dialysis (PD) although it has several drawbacks. Some of these drawbacks have been addressed by the introduction of solutions with low glucose degradation products and physiological pH in dual-chambered bags. Despite this achievement, there is a need for alternative osmotic agents. This randomized clinical trial analyzes 3-month's clinical experience with a mixture of 0.6% amino acids and 1.4% glycerol. Methods The study was performed at the renal units of the University Hospitals Ghent, Belgium, and Utrecht, The Netherlands. Stable PD patients were randomized for either protocol A (test solution, n = 5) or protocol B (control regimen, n = 5). In both protocols, there was a run-in phase of 1 month with a dialysis regimen of 2 × 2 L 2.27% glucose solution (Dianeal; Baxter, Nivelles, Belgium), 1 × 2 L Extraneal (Baxter), and 1 × 2 L glucose solution (Dianeal). After this month-long run-in period, patients in group A received during 3 months 2 × 2 L amino acid/glycerol solution, 1 × 2 L Extraneal, and at least 1 × 2 L of a classic glucose solution. Results Glucose absorption decreased in the test group during the test phase (from 84.2 ± 8.7 to 11.7 ± 11.6 g/24 hours, p = 0.001). Dialysate levels of cancer antigen 125 (CA125) increased in the test group, from 17.5 ± 11.0 to 32.4 ± 4.6 units/L ( p = 0.04), whereas, in the control group, the levels remained stable (15.5 ± 8.7 and 14.9 ± 9.8 units/L respectively, p = 0.4). There were no differences in serum urea, serum bicarbonate, serum osmolarity, serum albumin, or parameters related to skin-fold thickness or serum glycerol levels between control and test solutions. No differences were observed in obtained ultrafiltration after a 4-hour dwell with 2.27% glucose or the test solution, both measured at week 4 of the run-in period and week 12 of the test period. Conclusion This study demonstrated that the use of a new 0.6% amino acid/1.4% glycerol-containing dialysis solution is safe and well tolerated. Glucose load was reduced significantly and dialysate CA125 levels improved significantly. Ultrafiltration was comparable with that of a 2.27% glucose solution. All these factors, in combination with the potential nutritional benefits, can contribute to a beneficial impact on the success of the PD technique. Further long-term studies in larger patient groups are warranted to explore the potential of this promising new solution.

THE EFFICIENCY OF THE IODINE ORGANIC FORM IN BREEDING BOARS NUTRITION

2016 ◽  
Vol 1 (4) ◽  
pp. 74-79
Author(s):  
Надеев ◽  
Vasiliy Nadeev ◽  
Фомичев ◽  
Yuriy Fomichev ◽  
Никанова ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Physiological State ◽  
Iodine Content ◽  
The Body ◽  
Feed Additive ◽  
Control Group ◽  
Organic Form ◽  
Experimental Animals ◽  
Basic Diet

The purpose of research is improvement of clinical and physiological state of the organism breeding boars and prevention of iodine deficiency by introducing a basic diet of organic form bioiodine – feed additive «Prost». Production experience on breeding boars Landrace breed – research was conducted. By the principle of analogues, two boars group was formed. The animals were kept under identical conditions. All boars producers received the same feed SC-2 with the same content of metabolizable energy. Diet contain in its composition: 45% barley, 30% wheat; 5% corn; 20% of bvmd. Boars-producers of the control group fed a basic diet (RR) without additives, which conforms to the standards VIZH for this age group. The animal feed of the experimental group were injected with 200 mg per head per day of the feed additive «Prost». Blood for morfogematological studies were taken from the jugular vein at the same time, the chime before feeding. The scientific-production experiment studied the effect of the feed additive «simple» biochemical and morphological parameters of blood boars. As a result of studies, it was found that the inclusion in the diet of the treated group fodder additive, organic iodine content of globulins in the serum of experimental animals was by 5.1% higher than the control group, total protein 7.6%, respectively. The number of red blood cells in the animals in the group with the addition of a «simple» for the test period was higher by 22.1%, 22.1% of red blood cells, hematocrit 17.1%, compared to the control. The study of the white blood boars – when fed feed manufacturers «just» supplements in the experimental animals was observed decrease in the total number of leukocytes by 5.8%, which contributed to the increase of protective and adaptive reactions and reduced antigenic, toxicological stress for the body, the iodine content to an increase in whole blood by 43.8 and 246.3% for the bristles, thyroxine T4 6.5% compared with the control group.

scholarly journals Effects of Isoflavone Extracts on the Skin Improvement During the Seaweeds Peeling Massage

2021 ◽  
Vol 27 (4) ◽  
pp. 813-826
Author(s):  
Hyun-Kyoung Kim ◽  
Sang-Mo Kang
Keyword(s):  
Local Anesthetic ◽  
Recovery Period ◽  
Test Group ◽  
Skin Tone ◽  
Hair Follicles ◽  
Control Group ◽  
Measurement Results ◽  
Skin Conditions

This study was aimed to investigate the effects of isoflavone extracts on the skin improvement during the seaweeds peeling massage. Local anesthetic cream was applied to the control group (left face) with 20 persons and the seaweeds peeling massage was performed after 30 minutes for five minutes. After completion of the massage in the control group, 0.5 mL of isoflavone extract was applied to the test group (right face), and the seaweeds peeling massage was performed after five minutes for five minutes. After 28 days of skin recovery period, the degree of skin recovery was compared in both groups. With respect to the measurement results of skin conditions to compare the effects of seaweeds peeling, the levels of hair follicles (<i>p</i><0.01), wrinkles (<i>p</i><0.05), pigmentation (<i>p</i><0.05), sebum (<i>p</i><0.05), and porphyrin (<i>p</i><0.05) in the test group applied with isoflavone extract were lower than those in the control group applied with local anesthetic cream, while pigment under the eyes and skin tone were not different. The results of this study showed that isoflavone extract as a pretreatment was more effective to improve the conditions of hair follicles, wrinkles, pigmentation, sebum, and porphyrin than local anesthetic cream during the seaweeds peeling massage. Therefore, isoflavone extract can be considered to enhance the effects of seaweeds peeling massage.

scholarly journals Changes in clinical parameters following adjunctive local sodium hypochlorite gel in minimally invasive nonsurgical therapy (MINST) of periodontal pockets: a 6-month randomized controlled clinical trial

2021 ◽  
Author(s):  
Vincenzo Iorio-Siciliano ◽  
Luca Ramaglia ◽  
Gaetano Isola ◽  
Andrea Blasi ◽  
Giovanni E. Salvi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Minimally Invasive ◽  
Clinical Outcomes ◽  
Sodium Hypochlorite ◽  
Test Group ◽  
Bacterial Biofilm ◽  
Outcome Variable ◽  
Control Group ◽  
Mechanical Removal ◽  
Nonsurgical Therapy

Abstract Background The mechanical disruption and removal of the subgingival biofilm represent the most important step in the treatment of periodontitis. However, in deep periodontal pockets, mechanical removal of the subgingival biofilm is difficult and frequently incomplete. Preliminary findings indicate that the use of amino acid buffered sodium hypochlorite (NaOCl) gel may chemically destroy the bacterial biofilm and facilitate its mechanical removal. Objectives To clinically evaluate the efficacy of minimally invasive nonsurgical therapy (MINST) of periodontal pockets with or without local application of an amino acid buffered sodium hypochlorite (NaOCl) gel. Materials and methods Forty untreated patients diagnosed with severe/advanced periodontitis (i.e. stage III/IV) with a slow/moderate rate of progression (i.e. grade A/B) were randomly allocated in two treatment groups. In the test group, the periodontal pockets were treated by means of MINST and NaOCl gel application, while in the control group, treatment consisted of MINST alone. Full-mouth plaque scores (FMPS), full-mouth bleeding scores (FMBS), probing depths (PD), clinical attachment levels (CAL) and gingival recessions (GR) were assessed at baseline and at 6 months following therapy. The primary outcome variable was PD reduction at sites with PD ≥ 5 mm at baseline. Results At 6 months, statistically significant differences between the two groups were found (p = 0.001) in terms of PD and CAL change. No statistically significant differences were found in terms of GR (p = 0.81). The number of sites with PD ≥ 5 mm and BOP (+) decreased statistically significantly (p = 0.001), i.e. from 85.3 to 2.2% in the test group and from 81.6 to 7.3% in the control group, respectively. Statistically significant differences between test and control groups were recorded at 6 months (p = 0.001). MINST + NaOCl compared to MINST alone decreased statistically significantly (p = 0.001) the probability of residual PDs ≥ 5 mm with BOP− (14.5% vs 18.3%) and BOP+ (2.2% vs. 7.2%). Conclusions Within their limits, the present results indicate that (a) the use of MINST may represent a clinically valuable approach for nonsurgical therapy and (b) the application of NaOCl gel in conjunction with MINST may additionally improve the clinical outcomes compared to the use of MINST alone. Clinical relevance In patients with untreated periodontitis, treatment of deep pockets by means of MINST in conjunction with a NaOCl gel may represent a valuable approach to additionally improve the clinical outcomes obtained with MINST alone

A Common Thrombomodulin Amino Acid Dimorphism Is Associated with Myocardial Infarction

1997 ◽  
Vol 77 (02) ◽  
pp. 248-251 ◽  
Author(s):  
Lena Norlund ◽  
Johan Holm ◽  
Bengt Zöller ◽  
Ann-Kristin Öhlin
Keyword(s):  
Myocardial Infarction ◽  
Amino Acid ◽  
Plasma Concentration ◽  
Acute Coronary Syndromes ◽  
Protein C ◽  
Integral Membrane Protein ◽  
Control Group ◽  
Healthy Controls ◽  
Coronary Syndromes ◽  
Premature Myocardial Infarction

SummaryEndothelial dysfunction and haemostatic imbalance are believed to be important aetiological factors in the development of acute coronary syndromes. Thrombomodulin (TM) is an integral membrane protein crucial for normal endothelial function and activation of the protein C anticoagulant pathway. We have investigated the importance of a common C/T dimorphism in the TM gene (nucleotide 1418) for development of premature myocardial infarction (MI). The C/T dimorphism predicts an Ala455 to Val replacement in the sixth EGF-like domain of TM. The dimorphism was investigated in 97 MI survivors and 159 healthy controls. The C allele was significantly more frequent among patients than controls (p = 0.035). The allele frequency for the C allele was 0.82 in the patients and 0.72 in the control group. The plasma concentration of TM was investigated among healthy controls but was not related to the C/T dimorphism. In conclusion, the association of the C allele with premature MI, suggests that the TM gene and the C/T dimorphism may be aetiological factors involved in the pathogenesis of MI. Possibly, the Ala455 to Val replacement may affect the function of the TM molecule and the activation of the protein C anticoagulant pathway.

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