scholarly journals The influence of multiphytoadaptogen on leukocyte infiltration in mice hepatocarcinomas

2019 ◽  
Vol 18 (2) ◽  
pp. 60-65
Author(s):  
R. V. Karpova ◽  
E. V. Bocharov ◽  
O. A. Bocharova ◽  
I. V. Kazeev ◽  
V. G. Kucheryanu ◽  
...  
Keyword(s):  
Leukocyte Infiltration ◽  
Male Mice ◽  
Liquid Form ◽  
Liver Tumours ◽  
Tissue Samples ◽  
Experimental Animals ◽  
Tumour Formation ◽  
Hematoxylin And Eosin ◽  
Mice Liver ◽  
Therapeutic Administration

The aim of this study was the morphological investigation of the CBA mice liver tissue at different stages of ontogenesis as well as during liquid form multifitoadaptogen therapeutic administration.Materials and methods . The study objects are the liver samples of CBA male mice (subline CBA / LacY). Experimental animals received a 10 % solution of multifitoadaptogen from 6 months of age until natural death. Tissue samples were fixed in neutral buffered formalin and embedded in paraffin. Sections from paraffin blocks were stained with hematoxylin and eosin. The structure of the liver tumours was determined as well as the quantitаtive degree of leukocyte infiltration in the tumour tissue.Results . The tumours morphologically structured as moderately differentiated trabecular hepatocarcinomas (at 8 months of age) and low differentiated trabecular-acinar hepatocarcinomas (aged 22 months) were revealed in CBA male mice. Higher lever of leukocyte infiltration in hepatocarcinomas of experimental animals was determined.Conclusion . Leukocyte infiltration may be important for antitumour immune reaction as well as for reduction of the tumour formation incidence in high-cancer mice.

scholarly journals Excretion and binding of tritium-labelled oestradiol in mice (Mus musculus): implications for the Bruce effect

Reproduction ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 255-263 ◽  
Author(s):  
Adam C Guzzo ◽  
Robert G Berger ◽  
Denys deCatanzaro
Keyword(s):  
Cerebral Cortex ◽  
Intranasal Administration ◽  
Male Mice ◽  
Olfactory Bulbs ◽  
Tissue Samples ◽  
Mouse Urine ◽  
Osmotic Pumps ◽  
Bruce Effect ◽  
Blastocyst Implantation ◽  
Pre Treatment

Male mouse urine contains 17β-oestradiol (E2) and other steroids. Given that males actively direct urine at proximate females and intrauterine implantation of blastocysts is vulnerable to minute amounts of exogenous oestrogens, males' capacity to disrupt early pregnancy could be mediated by steroids in their urine. When male mice were implanted with osmotic pumps containing tritium-labelled E2 (3H-E2) or injected i.p. with 3H-E2, radioactivity was reliably detected in their urine. Following intranasal administration of 3H-E2 to inseminated females, radioactivity was detected in diverse tissue samples, with there being significantly more in reproductive tissues than in brain tissues. When urine was taken from males injected with 3H-E2, and then intranasally administered to inseminated females, radioactivity was detected in the uterus, olfactory bulbs, and mesencephalon and diencephalon (MC+DC). When inseminated and ovariectomised females were perfused at the point of killing to remove blood from tissues, more radioactivity was detected in the uterus than in muscle, olfactory bulbs, MC+DC and cerebral cortex. Pre-treatment with unlabelled E2 significantly reduced the uptake of 3H-E2 in the uterus. Taken with evidence that males deliver their urine to the nasal area of females, these results indicate that male urinary E2 arrives in tissues, including the uterus, where it could lead to the disruption of blastocyst implantation.

scholarly journals Immunohistochemical WWOX Expression and Association with Angiogenesis, p53 Expression, Cell Proliferation and Clinicopathological Parameters in Cervical Cancer

2018 ◽  
Vol 40 (02) ◽  
pp. 079-085 ◽  
Author(s):  
Mariana Seabra ◽  
Eduardo Cândido ◽  
Paula Vidigal ◽  
Rivia Lamaita ◽  
Angélica Rodrigues ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cervical Squamous Cell Carcinoma ◽  
Pelvic Lymphadenectomy ◽  
Histological Evaluation ◽  
Clinicopathological Parameters ◽  
Control Group ◽  
Ki 67 ◽  
Tissue Samples ◽  
P53 Expression ◽  
Hematoxylin And Eosin

Objective The current study evaluated the expression of WW domain-containing oxidoreductase (WWOX), its association with clinicopathological features and with p53, Ki-67 (cell proliferation) and CD31 (angiogenesis) expression in patients with invasive cervical squamous cell carcinoma (ICSCC). To the best of our knowledge, no other study has evaluated this association. Methods Women with IB stage-ICSCC (n = 20) and women with uterine leiomyoma (n = 20) were prospectively evaluated. Patients with ICSCC were submitted to type B-C1 radical hysterectomy and pelvic lymphadenectomy. Patients in the control group underwent vaginal hysterectomy. Tissue samples were stained with hematoxylin and eosin for histological evaluation and protein expression was detected by immunohistochemistry studies. Results The WWOX expression was significantly lower in the tumor compared with the expression in the benign cervix (p = 0.019). The WWOX expression was inversely associated with the CD31 expression in the tumor samples (p = 0.018). There was no association between the WWOX expression with the p53 expression (p = 0.464) or the Ki-67 expression (p = 0.360) in the samples of invasive carcinoma of the cervix. There was no association between the WWOX expression and tumor size (p = 0.156), grade of differentiation (p = 0.914), presence of lymphatic vascular invasion (p = 0.155), parametrium involvement (p = 0.421) or pelvic lymph node metastasis (p = 0.310) in ICSCC tissue samples. Conclusion The results suggested that WWOX may be involved in ICSCC carcinogenesis, and this marker was associated with tumor angiogenesis.

scholarly journals Impact of sex, androgens, and prostate size on C57BL/6J mouse urinary physiology: urethral histology

2020 ◽  
Vol 318 (3) ◽  
pp. F617-F627
Author(s):  
Hannah Ruetten ◽  
Kyle A. Wegner ◽  
Conner L. Kennedy ◽  
Anne Turco ◽  
Helen L. Zhang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Count ◽  
National Institutes Of Health ◽  
Male Mice ◽  
Male And Female ◽  
Future Studies ◽  
Biological Variable ◽  
Prostate Size ◽  
Hematoxylin And Eosin ◽  
The Impact

The National Institutes of Health leveled new focus on sex as a biological variable with the goal of understanding sex-specific differences in health and physiology. We previously published a functional assessment of the impact of sex, androgens, and prostate size on C57BL/6J mouse urinary physiology (Ruetten H, Wegner KA, Zhang HL, Wang P, Sandhu J, Sandhu S, Mueller B, Wang Z, Macoska J, Peterson RE, Bjorling DE, Ricke WA, Marker PC, Vezina CM. Am J Physiol Renal Physiol 317: F996–F1009, 2019). Here, we measured and compared five characteristics of urethral histology (urethral lumen diameter and area, epithelial cell count, epithelial and rhabdosphincter thickness, epithelial cell area, and total urethral area) in male and female 9-wk-old C57BL/6J mice using hematoxylin and eosin staining. We also compared male mice with castrated male mice, male and female mice treated with the steroid 5α-reductase inhibitor finasteride or testosterone, or male mice harboring alleles ( Pbsn4cre/+; R26RDta/+) that reduce prostate lobe mass. The three methods used to reduce prostate mass (castration, finasteride, and Pbsn4cre/+; R26RDta/+) changed urethral histology, but none feminized male urethral histology (increased urethral epithelial area). Exogenous testosterone caused increased epithelial cell count in intact females but did not masculinize female urethral histology (decrease epithelial area). Our results lay a critical foundation for future studies as we begin to parse out the influence of hormones and cellular morphology on male and female urinary function.

TLR2 affects CD86 expression and inflammatory response in burn injury mice through regulation of p38

2017 ◽  
Vol 95 (5) ◽  
pp. 549-555 ◽  
Author(s):  
Li Li ◽  
Gang Xu ◽  
Chenwang Duan
Keyword(s):  
Inflammatory Response ◽  
Burn Injury ◽  
Vital Role ◽  
Mutant Type ◽  
Tissue Samples ◽  
Tlr2 Agonist ◽  
P38 Inhibitor ◽  
Tnf Α ◽  
Hematoxylin And Eosin

The aim of this study was to assess the effects of TLR2–p38–CD86 signaling pathways on the inflammatory response in a mouse model of burn injury. Wild-type (TLR2+/+) and mutant-type (TLR2−/−) mice were obtained, and a mouse burn injury model was constructed. Tissue samples were examined with hematoxylin and eosin staining and the transferase mediated nick end labeling (TUNEL) method. Macrophages were treated with TLR2 agonist and p38 inhibitor. The expression levels of TLR2, p38, CD86, IL-1β, and TNF-α were quantified by RT-qPCR, Western blot, and ELISA. When compared with the sham group, the burn group had a significantly higher rate of apoptosis as well as higher expressions of TLR2, p38, CD86, IL-1β, and TNF-α. Inhibiting TLR2 was shown to significantly reduce the expressions of p-p38, CD86, IL-1β, and TNF-α. In the results of in-vitro experiments, TLR2 agonist increased the expression of p-p38, CD86, IL-1β, and TNF-α, whereas a p38 inhibitor was shown to reduce the expression of CD86, IL-1β, and TNF-α. Our results suggest that the TLR2–p38–CD86 signaling pathway plays a vital role in inflammation associated with burn injury.

scholarly journals Hepatoprotective and Antioxidative Activities ofCornus officinalisagainst Acetaminophen-Induced Hepatotoxicity in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nam-Hun Lee ◽  
Chang-Seob Seo ◽  
Ho-young Lee ◽  
Da-Young Jung ◽  
Jun-Kyung Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Serum Marker ◽  
Marker Enzymes ◽  
Asian Countries ◽  
Cornus Officinalis ◽  
Collagen Accumulation ◽  
Hematoxylin And Eosin ◽  
Mice Liver ◽  
Hepatic Injuries

The fruit ofCornus officinalisSieb. et Zucc. is commonly prescribed in Asian countries as a tonic formula. In this study, the hepatoprotective effect of ethanolic extracts of the fruit ofC. officinalis(ECO) was investigated in a mouse model of acetaminophen- (APAP-) induced liver injury. Pretreatment of mice with ECO (100, 250, and 500 mg/kg for 7 days) significantly prevented the APAP (200 mg/kg) induced hepatic damage as indicated by the serum marker enzymes (AST, ALT, and LDH). Parallel to these changes, ECO treatment also prevented APAP-induced oxidative stress in the mice liver by inhibiting lipid peroxidation (MDA) and restoring the levels of antioxidant enzymes (SOD, CAT, and HO-1) and glutathione. Liver injury and collagen accumulation were assessed using histological studies by hematoxylin and eosin staining. Our results indicate that ECO can prevent hepatic injuries associated with APAP-induced hepatotoxicity by preventing or alleviating oxidative stress.

scholarly journals Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 35-40
Author(s):  
Yuandani ◽  
Silvia Mardaliza ◽  
Marianne
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepatoprotective Effect ◽  
Hepatic Tissue ◽  
High Dose ◽  
Male Mice ◽  
Mice Liver

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice  received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw .   Keywords: C. mangga, rhizomes, biochemistry parameters, histopathology

scholarly journals Comparative Anatomical Studies on Ductus Venosus in Fetuses of Domestic Ruminants

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Pamela Bejdić ◽  
Amel Ćutuk ◽  
Amer Alić ◽  
Benjamin Čengić ◽  
Rizah Avdić ◽  
...  
Keyword(s):  
Histological Examination ◽  
Umbilical Vein ◽  
Circulatory System ◽  
Fetal Tissue ◽  
Ductus Venosus ◽  
Elastic Fibers ◽  
Silver Stain ◽  
Tissue Samples ◽  
Domestic Ruminants ◽  
Hematoxylin And Eosin

AbstractThe study has aimed to investigate and determine the anatomical position, shape, size, and histological features of the ductus venosus, and its role as a shunt in the fetal circulatory system in domestic ruminants. The research was conducted on 19 bovine, 11 sheep and 5 goat fetuses, aborted at the late stage of pregnancy or deceased just after delivery. The general anatomy of the ductus venosus was investigated by in-situ dissection of the corrosive cast obtained by injection of 25% solution of Vinylite mass through the umbilical vein. For histological examination, the fetal tissue samples were stained with Hematoxylin and Eosin, Masson’s trichrome, Verhoeff-Van Gieson and Gomori's silver stain. The results showed that ruminant fetal ductus venosus is a curved, trumpet-shaped vessel, situated in the central part of the liver, above the porta hepatis. Its ventral part is constricted in the form of an isthmus, having a prominent lip-like thickening at the junction with the portal sinus. Histological examination showed the dominant presence of collagen and elastic fibers in its tunica media, with thin bands of smooth muscle fibers oriented in a longitudinal and circular direction indicating ability for vasoconstriction and vasodilatation.

scholarly journals Study of the anti-infl ammatory activity of the Potentilla anserina L on burn wounds in mice

2021 ◽  
pp. 113-116
Author(s):  
E.E. Savelieva ◽  
◽  
O.F. Veselova ◽  
I.V. Gackih ◽  
V.A. Horgevski ◽  
...  
Keyword(s):  
White Male ◽  
Hair Follicles ◽  
Thermal Burn ◽  
Experimental Animals ◽  
Burn Wounds ◽  
Percolation Method ◽  
Potentilla Anserina ◽  
Entire Experiment ◽  
Hematoxylin And Eosin ◽  
Active Processes

The aim of the research. To investigate the anti-infl ammatory eff ect of Cinquefoil on a model of a thermal wound. Material and methods. A liquid extract in 70 % ethyl alcohol was obtained from dry crushed goose cinquefoil by the percolation method. Th e resulting extracts were evaporated under vacuum at a temperature of 50 °C. Th e dry residue was dissolved in water immediately before treatment at a dose of 250 mg / kg. Th e evaluation of anti-infl ammatory activity was carried out in an experiment on 20 white male mice. Th ermal skin burns were simulated in all animals. Th e burns were healed in an open way. During the entire experiment, the general condition of the experimental animals was assessed, body weight and temperature were determined, the appearance of wounds, appetite, and activity were assessed. For histological examination, a section of the skin was taken, including the burn zones and intact tissues. Paraffi n sections were stained with hematoxylin and eosin. Results. An analysis of the results of the experimental study showed that all experimental animals, under the infl uence of high temperatures, developed a third degree thermal burn of the skin, characterized by damage to the entire thickness of the skin with complete death of hair follicles, sweat and sebaceous glands. At the site of the burn, the skin was dense, motionless and insensitive to painful stimuli. Th e appearance of the wounds did not diff er between groups of animals. Comparative microscopy of the control and experimental groups showed that the depth of damage to the epidermis and dermis was more pronounced in the experimental group. In addition, in the same group, lymphocytic infi ltration of the dermis, hypodermis, the presence of a pronounced vascular reaction and edema of the dermis are more pronounced, which indicates a more pronounced infl ammatory. Conclusion. Th e analysis of the results obtained shows that in the treatment of burn wounds, the local application of the preparation Potentilla goose on the 4th day in the experiment does not lead to a pronounced anti-infl ammatory eff ect. However, the revealed lymphocytic infi ltration of the dermis and other histological parameters indicate more active processes in the tissues, which may subsequently lead to more accelerated wound cleansing and faster regeneration

scholarly journals The effects of kumiss supplementation on body weight, food intake, nesfatin-1 and irisin levels in BALB/C mice

2020 ◽  
Vol 90 (6) ◽  
pp. 627-636
Author(s):  
Canan Gulmez ◽  
◽  
Onur Atakisi
Keyword(s):  
Weight Loss ◽  
Food Intake ◽  
Live Weight ◽  
Feed Consumption ◽  
Control Group ◽  
Male Mice ◽  
Tissue Samples ◽  
Bacterial Colony ◽  
Metabolic Functions ◽  
Significant Difference

The aim of this study was to investigate the plasma and tissue levels of nesfatin-1 and irisin hormones, which were discovered in recent years and are associated with endocrine and metabolic functions, in kumiss-supplemented mice. Sixteen BALB/C male mice were divided into two groups as control and kumiss groups. During the experiment, the kumiss was added to the drinking water of mice at a ratio of 1:1 to obtain a daily 2×108 cfu/mL bacterial colony, and was given once a day orally for 20 weeks. The weights and food intake of the animals were monitored during the experiment. The nesfatin-1 and irisin levels in plasma and tissue samples were determined using ELISA kits. Kumiss supplementation reduced the live weight for 2-12 weeks (P<0.05). However, no significant difference was observed after the 12th week. The feed consumption of the kumiss group was lower at the beginning and the 10th week, and at the end, compared to the control group (P<0.05). The plasma levels of nesfatin-1 and irisin (P<0.001) decreased while the liver levels increased (P<0.05 and P<0.001, respectively). The results indicate that plasma and liver levels of nesfatin-1 and irisin are regulated by diet and are effective in weight loss and food intake.

scholarly journals Immunohistochemical Expression of P16 Protein and TGF β1 in Mice Liver Exposed to Fumonisin B1

2020 ◽  
Vol 17 (2) ◽  
pp. 0401
Author(s):  
Sinai Mohammed et al.
Keyword(s):  
Fusarium Species ◽  
Critical Role ◽  
Fumonisin B1 ◽  
Structural Similarity ◽  
Saline Control ◽  
Control Group ◽  
Male Mice ◽  
P16 Protein ◽  
Mice Liver ◽  
Tgf Β1

Fumonisin B1 (FB1) is a mycotoxin produced in some grains (mainly corn) by Fusarium species. Due to a structural similarity between FB1 and sphinganine, sphingolipids metabolism is inhibited. Such inhibition plays a critical role in cell to cell singling and structure of lipoprotein; therefore FB1 has been suggested to have a relationship with human and animal cancer. This research is planned to study the effect of FB1 on male mice at two doses (20 and 30 µg/ ml) on the expression of TGF-β1 and p16 in liver cells. Three groups of Swiss albino male mice; each group was orally administrated with FB1 toxin as the following: normal saline (control group); 20 and 30 µg/ ml. All groups were sacrificed after two weeks of oral management. Liver samples were collected and prepared for immunohistochemistry technique (IHC) using anti-TGF-β1 and anti-p16 antibodies. The results showed that exposure to FB1 caused significant elevation of TGF-β1 in both doses (76.74 ± 2.387% and 80.62 ± 7.277%, respectively) in comparison with the control group (46.79 ± 2.404%). The level of p16 protein was decreased at 20 µg/ml (76.63 ± 2.349%) and then increased at 30 µg/ml (81.25 ± 6.263%) but the expression was lower than that of control (90.00 ± 0.805%). In conclusion, FB1 has a significant effect on TGF-β1 and p16 protein expression at both doses (20 and 30 µg/ml), and therefore, its role in cancer development is suggested.

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