scholarly journals THE MORPHOLOGY OF THE ACCIDENTAL INVOLUTION OF THE THYMUS AT THE EXPERIMENTAL CANCEROGENESIS

2019 ◽  
Vol 27 (1) ◽  
pp. 30-35
Author(s):  
A. A. Kotyolkina ◽  
L. M. Merkulova ◽  
O. Yu. Kostrova ◽  
G. Yu. Struchko
Keyword(s):  
Immune System ◽  
Mast Cells ◽  
Biogenic Amines ◽  
The Body ◽  
Lymphoid Cells ◽  
Combined Effect ◽  
Structural Reorganization ◽  
Experimental Carcinogenesis ◽  
Two Factors ◽  
S 100

The development of tumors in the body is a powerful factor in the dysfunction of the immune system. The features of involution processes in the thymus in experimental carcinogenesis are due to impaired metabolism in the body as a whole and the production of lymphoid cells with altered functions. The purpose of the study is to summarize data from modern scientific literature and own research on the accidental transformation of the thymus and the reaction of its cellular structures against the background of experimental carcinogenesis with the development of a mammary gland tumor, stress and the combined effect of these factors. Based on the analysis, it was found that dendritic and mast cells of the thymus respond most actively to tumor growth and stress. Morphometric parameters and changes in organ's cytoarchitecture correspond to the phases of the development of accidental involution, which is most pronounced with the combined effect of two factors. A significant role in these processes belongs to biogenic amines and hormones. The introduction of carcinogens leads to a structural reorganization of the gland: the lobules become more elongated, the interlobular spaces increase, the thickness of the cortical and the area of the medulla increase. There is a decrease in the number of S-100+-cells, an increase in the number of mast cells with a predominance of degranulated and completely devastated forms. The level of histamine in the cells of the pre-medullar area decreases in 1,3 times, in the thymocytes of the cortex and medulla in 1,7 times. Along with the change in the level of biogenic amines, there is a decrease in the ratio of their ratio, which indicates an increase in the functional activity of cells. Against the background of stress, as an additional factor that depresses the immune system, there is a pronounced disorganization of the thymus lobes: a decrease in the thickness of the cortical and the area of the medulla, expansion and filling of the interlobular connective tissue septa with adipose tissue. The number of dendritic cells also decreases and the content of degranulated and completely devastated forms of mast cells increases, the level of histamine significantly decreases in all structures. Thus, data from the literature and our own research indicate that the combined effect of carcinogenesis and stress leads to more pronounced changes in the structure of the thymus in the process of accidental involution and the structural specificity of its manifestations.

scholarly journals FEATURES OF RAT THYMUS MORPHOLOGY AT THE INDUCTION OF THE BREAST CANCER BY N-METHYL-N-NITROSOUREA

2020 ◽  
Vol 28 (2) ◽  
pp. 55-63
Author(s):  
Anastasiya A. Kotyolkina ◽  
Larisa M. Merkulova ◽  
Ol'ga Yu. Kostrova ◽  
Gleb Yu. Struchko ◽  
Evgeny V. Moskvichev ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dendritic Cells ◽  
Mast Cells ◽  
Biogenic Amines ◽  
Functional Activity ◽  
Breast Tumor ◽  
Subsequent Development ◽  
Female Rats ◽  
Quantitative Composition ◽  
Experimental Carcinogenesis

The immune system as a whole plays a leading role in the formation of an adequate immune response during the onset and development of tumors. The aim of the study was to reveal the features of the change in the morphology and level of biogenic amines of the thymus of rats during experimental carcinogenesis of breast cancer by the injection of N-methyl-N-nitrosourea. In the work used luminescence-histochemical, general histological and immunohistochemical methods, which made it possible to obtain important information about the functional activity of the cellular elements of the thymus, the quantitative composition of macrophages, dendritic cells, lymphocytes and the qualitative characteristics of mast cells. The experiment was carried out on 85 white nonlinear female rats, which were injected with the carcinogen N-methyl-N-nitrosourea, which induces a breast tumor. Animals were divided into two groups: intact (n=30) and rats injected with a carcinogen (n=45). The thymus structure was examined 1, 2, and 3 months after the end of the injections. During the study, changes in the morphometric parameters of the organ are noted. A month after the injection of the drug, the area of the medulla of the thymus and the thickness of the cortex substance of the organ increases, which decrease with an increase in the study period. Replacement of the thymus parenchyma with adipose and connective tissue begins after 2 months and progresses with an increase in the study period. A tendency to wavelike changes in biogenic amines was revealed, after 1 month there is a significant increase in the level of histamine and a decrease in serotonin and catecholamines, after 2 months an increase in all biogenic amines, especially histamine (1.5 times or more) in premedullary and mast cells, thymocytes of the cortex. After 3 months, on the contrary, there is a decrease in histamine and an increase in the level of monoamines. The ratio (serotonin+histamine)/catecholamines after 1 month indicate a decrease in the functional activity of the thymus, and after 3 months - about its increase. At all stages of the study, degranulated forms of mast cells predominate. In the dynamics of the experiment, an increase in the number of dendritic cells, lymphocytes, and twice the number of macrophages and Bcl-2+cells was revealed. The data obtained in the course of the study suggested that the introduction of N-methyl-N-nitrosourea and the subsequent development of a breast tumor is reflected in the change in the morphofunctional state of the thymus. In this case, the greatest changes were observed 2 months after the end of the administration of the carcinogen, even before the formation of a breast tumor. The data obtained can have a certain value in determining the immune status in models of experimental carcinogenesis.

scholarly journals THE DIAGNOSIS OF DAUCUS CAROTA AS ALERGOGEN ON THE IMMUNE SYSTEM IN WHITE LABORATORY RAT

2019 ◽  
Vol 31 (4) ◽  
pp. 969-974
Author(s):  
Mire Spasov ◽  
Icko Gjorgoski
Keyword(s):  
Immune System ◽  
Mast Cells ◽  
Allergic Reaction ◽  
External Environment ◽  
Blood Parameters ◽  
The Body ◽  
Type I ◽  
Ige Antibodies ◽  
White Rats ◽  
Type I Hypersensitivity

The allergic reaction or type I hypersensitivity is a hypersensitive disorder to the immune system, which occurs by ingress of non-pathogenic agents from the external environment in the body. Antigens, in this case allergens, are substances from the environment that are harmless to most people. In allergies there is an inherent tendency to inherit the genes that make these people susceptible to allergies. Rapid sensitization may occur as a local reaction, which is just unpleasant (seasonal rhinitis or hay fever), severe exhaustion (asthma), or culminating in a fatal systemic disorder (anaphylaxis). Allergens in the body are inserted by inhalation, ingestion or injection, and move to mucous membranes, where they are accepted by T-lymphocytes. TN2 lymphocytes produce IL-4, which stimulate B-lymphocytes to differentiate into plasma cells. These cells excrete IgE, which recognize allergens. Excreted IgE antibodies sensitize mast cells that originate from the bone marrow. When sensitized individuals again expose themselves to an allergen from the external environment, they bind to specific IgE-antibodies to the mast cells (memory cells), whereby various mediators are excreted, causing inflammatory response, mucus secretion, vasoconstriction of blood vessels, and spasm of the airways. The aim of the study was to investigate the allergenic effect of Daucus carotte on the change in the number of leukocytes, lymphocytes, monocytes, granulocytes, basophils, and immunoglobulins as important components of the immune system. From the pollen of this plant, recombinant allergen is extracted, in the form of injections with a volume of 150μI. In the experiments, as experimental models were used Wistar white rats at the age of 6 to 9 weeks. We injected the allergen into the first, second, third and fourth week in a group of 6 rats in an amount of 5 μl and a second group of 6 rats in an amount of 2.5 μl allergen absorbed in 100 μl AI (OH) 3 (Serva, Heidelburg, Germany, 2 μg / mI) in a total volume of 150μI sterile PbS. The third group of 6 rats was a control group. The results showed that the Dacus carota causes an allergic reaction in Wistar white rats and its intensity depends directly on the volume of the allergen and the individuals that come into contact with it. Once we compared the values of blood parameters, leukocytes, lymphocytes, monocytes, granulocytes, and basophils, as well as the IgG, IgG1, IgG2a, and IgE IgG, IgG1, IgG2a, and IgE concentrations, we concluded that the higher concentration of Daucus carota causes a higher elevation in blood parameters and concentrations of immunoglobulins, compared to the smaller concentration of the same allergen. From the studies conducted over a period of one month, it was found that Dacus carota causes an allergic reaction, which is classified in Type I hypersensitivity in white laboratory rats of the Wistar strain.

scholarly journals Effect of Bone Marrow Allotransplantation on Neurotransmitter Structures of Lymph Nodes

2021 ◽  
Vol 10 (1) ◽  
pp. 54-62
Author(s):  
V. O. Romanov ◽  
L. A. Lyubovtseva ◽  
O. V. Vorob'eva ◽  
L. P. Romanova
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Lymph Nodes ◽  
Biogenic Amines ◽  
Sharp Decrease ◽  
Lymphoid Cells ◽  
Male Rats ◽  
Special Role ◽  
Ki 67 ◽  
Cervical Lymph Nodes

Despite the many factors affecting the state of the lymph nodes, a special role is played by biogenic amines synthesized by the cells of the APUD system and specialized macrophages, which in the context of this study are designated as granular luminescent cells (GLC), as well as mast cells (MC). The presence of receptors for biogenic amines on lymphoid cells proves the subordination of the immune system to neurohumoral effects.The aim of the study was to identify neuroamine-containing cells in the lymph nodes and to determine the content of catecholamines (CA) and serotonin (ST) in them early after bone marrow allotransplantation (ALT).Material and methods. The experiment was carried out on outbred male rats. The 1st group consisted of intact animals (n=10), the 2nd – control individuals, which were injected with 3.0 ml of 0.85% sodium chloride solution (n=10); 3rd – animals (n=30), which underwent bone marrow ALT. To identify neuroamine-containing cells, a luminescence-histochemical study was carried out according to Falk–Hillarp method in Krokhina modification (1969), the proliferative activity of cells was assessed using an immunohistochemical reaction for Ki-67. Statistical analysis was performed using the Statistica-6 program.Research results. After 40 min in the GLC of the corona of the lymph nodes, an increase in the content of CA and ST by a factor of 1.2 was observed; after 2 hours, the CA content increased 1.7 times, and ST – 1.4 times, and after 4 hours there was a sharp decrease in the CA content by 3.6 times. In the GLC of the reactive center and in the reticuloendothelial GLC, the content of both CA and ST decreased. In the GLC of the paracortical zone, the content of CA and ST increased, and after 4 hours their decay occurred. In MC, the content of CA and ST decreased. MC degranulation was observed against the background of a sharp decrease in their number. Due to the degranulation of MC, starting from the 40th min after ALT, neuroamines accumulated in the intercellular space. After ALT, the Ki-67 index changed at different periods of the experiment, in the marginal sinuses it decreased by 13%, in the intermediate sinuses – by 61%, in the lymph nodules – by 47%, and in the paracortical zone – by 40%.Conclusion. Thus, bone marrow allotransplantation leads to a change in the number of granular luminescent and mast cells, as well as the content of biogenic amines in the cervical lymph nodes.

scholarly journals THE DIAGNOSIS OF PHLEUM PRATENSE AS ALERGOGEN ON THE IMMUNE SYSTEM IN WHITE LABORATORY RAT

2019 ◽  
Vol 31 (4) ◽  
pp. 975-980
Author(s):  
Hristijan Spasov ◽  
Mire Spasov ◽  
Icko Gjorgoski ◽  
Majlinda Ademi
Keyword(s):  
Immune System ◽  
Mast Cells ◽  
Allergic Reaction ◽  
Reference Value ◽  
Phleum Pratense ◽  
The Body ◽  
Recombinant Allergen ◽  
Type I ◽  
Allergic Reactions ◽  
Laboratory Rat

Allergic reactions in the body are initiated by non-pathogenic allergens that cause hypersensitivity to the immune system of the type I hypersensitivity group, characterized by excessive activation of the cells of the white blood cell, mast cells and basophils by IgE, resulting in an inflammatory response. The particles of the allergen in the body mainly penetrate the mucous membranes of the body openings causing a series of reactions by activating T cells and B cells. The main symptoms of allergic reactions are secretion from the mucous membrane, irritation, swelling with itching and redness. The aim of the research came from the assume that in the white laboratory rat, when treated with an allergen isolated from the pollen of the Phleum pratense plant, there may be an allergic reaction, and hence a change in some parameters of the immune system.As a result of these assumptions the following specific goals were set. To determine the change in the concentration of immunoglobulins in serum from the blood taken from the rats every week for a month, to determine the variation in the total number of leukocytes, determine the number of basophils that compared to the reference value will indicate whether there are certain changes. The basophils were the target of the study because it is known that their number is the same with the number of mast cells that also increase during the allergic reaction. Our goal was also to determine the changes in the above parameters in relation to the different concentration of the allergen.As an experimental model we used white laboratory rats from the Wistar kind.All of them were female at the age of 6 to 9 weeks. The chosen age is due to their genetic predisposition to develop Th2-type cells that are involved in the immune response to the model of the allergy that has been studied. The rats were divided into three groups of six animals, the first group of rats being treated with an allergen in a quantity of 5 μL, adsorbed in 100 μl Al (OH) 3 (Serva, Heidelburg, Germany, 2 mg / mL) in a total volume of 150 μl sterile PbS., treated with a recombinant allergen from Phleum pratense at room temperature, a second group treated with an allergen in an amount of 2.5 μl, and a third group of control rats that are not treated and serve to compare the results. Our studies have shown that in the treatment with an allergen isolated from the pollen of the plant Phleum pratense at concentrations of 5 μl and 2.5 μl,the white laboratory rat develops an allergic reaction with a change in the concentration of immunoglobulins, changes in the total number of leucocytes, the percentage of lymphocytes , monocytes and basophils. The results obtained by the ELISA test of the two volumes show that the concentrations of immunoglobulins during the experimental period of day by day, are continuously increasing, and are proportional to the volume of the allergen.

MECHANISMS AND FEATURES OF IMMUNE STATUS AND "OXIDATIVE STRESS - ANTIOXIDANT PROTECTION" SYSTEM IN PATIENTS WITH TUBERCULOSIS

2020 ◽  
pp. 16-20
Author(s):  
I. I. Hrek ◽  
Maryna Mykolayivna Kochuieva
Keyword(s):  
Oxidative Stress ◽  
Immune System ◽  
Immune Status ◽  
The Body ◽  
Lymphoid Cells ◽  
Protection System ◽  
Natural Resistance ◽  
Antioxidant Protection ◽  
Humoral Factors ◽  
Antioxidant Protection System

The study of the human immune system state in infection with M. tuberculosis is relevant because the course and outcome of this disease are largely determined by the immune status of the patient. However, 98 % of patients with pulmonary tuberculosis have an immune imbalance. It is known that in the protection against tuberculosis an important role belongs to the body's natural resistance, which is provided by a variety of cellular and humoral factors, physicochemical characteristics of tissues, lymphoid cells, leukocyte and macrophage responses and genetic resistance. When mycobacteria enter the body, polymorphonuclear leukocytes, monocytes and macrophages are the main phagocytic cells. Optimally high level of resistance to the pathogen develops only in the coordinated interaction of T−lymphocytes with macrophages. Studies of cellular immunity and genetic markers have shown that the course of tuberculosis infection is associated with suppression of their functional activity. Immune response deregulation is closely related to oxidative stress, which results from an imbalance between free reactive oxygen species and antioxidant mechanisms, with a higher risk of developing it rather in lungs than other organs. Many studies have presented the results of studying the state of the immune system and the "oxidative stress − antioxidant protection" system in tuberculosis. This is an important component, because the clinical course and outcome of treatment is largely determined by the status of these systems. A number of experts point out that the study of immunological and oxidative parameters in tuberculosis is of a great importance for deciding on the tactics of treatment and the choice of direction of influence on the course of the disease. Key words: M. Tuberculosis, immunity in tuberculosis, oxidative stress, antioxidant protection.

Studies on the Characterization of Factor VIII and a Co-factor VIII

1974 ◽  
Vol 31 (02) ◽  
pp. 328-338
Author(s):  
M. M. P Paulssen ◽  
H. L. M. A Vandenbussche-Scheffers ◽  
P. B Spaan ◽  
T de Jong ◽  
M. C Planje
Keyword(s):  
Molecular Weight ◽  
Factor Viii ◽  
The Body ◽  
Haemophilia A ◽  
Von Willebrand's Disease ◽  
Von Willebrand’S Disease ◽  
Polysaccharide Content ◽  
Plasma Factor ◽  
Two Factors

SummaryFactor VIII occurs in the body in two different forms. In lymph factor VIII is bound to chylomicra. In plasma, factor VIII is bound to a protein.After delipidation of chylomicra we obtained a glycoprotein with a high polysaccharide content and a molecular weight of approx. 160,000.In plasma, factor VIII is attached to a protein which is present in normal concentrations in plasma of patients with haemophilia A and in serum (co-factor VIII).This factor is deficient in both the plasma and the serum of patients with von Willebrand’s disease.The binding between factor VIII and co-factor VIII is reversible.Some properties of these two factors are described.

Immunity boosting diet during Covid 19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 832-838
Author(s):  
Roshna Sukheoji Bhutada ◽  
Renu Rathi ◽  
Devyani Dasar
Keyword(s):  
Immune System ◽  
Food Studies ◽  
Egg Yolk ◽  
The Body ◽  
Leafy Vegetables ◽  
Green Leafy Vegetables ◽  
Custard Apple ◽  
The Right ◽  
Daily Physical Activities ◽  
Physiological Reactions

WHO declared Covid 19 /SARS -COV-2 as a global pandemic.Till date, there is no medicine for COVID-19. If the Infection arises in the body then the defence mechanism activated against infection. A recent study suggests that temporarily augmenting the body's immune system in the early stages of COVID-19 can help patient to avoid severe symptoms as it is rightly said prevention is better than cure. Ayurveda approaches to develop physiological reactions to facilitate immunity. Planning of diet is most important to boost immunity.As per many researches to provide supplementary food which contains Zinc, Vitamin C,Vitamin D and immunity boosting foodsuch as citrus natural products, custard apple, apple, papaya is among the Fruits. Vegetables include broccoli, onion, garlic and green leafy vegetables. Nuts, ginger, turmeric, pepper, egg yolk, shellfish, mushroom. The need of the hour is a quick boost to immune system to keep it fit, fighting. One should get the right amount of nutrients from the diet, supplementation regimen to boost immune system.In this review, there are few common supplements and super food studies have been included. It might be a torch bearer as sample menu and their alternatives are given for a normal adult. Needy may change contemplated according to age, sex, body mass index and daily physical activities.

Nutritional Recommendation for COVID-19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 753-757
Author(s):  
Anagha Gulhane ◽  
Shamli Hiware
Keyword(s):  
Immune System ◽  
Global Health ◽  
Infectious Diseases ◽  
Immune Function ◽  
The Body ◽  
Health Issue ◽  
System Quality ◽  
Life Phase ◽  
Global Health Issue ◽  
Nutritional Recommendation

It is the most unreliable truth that anybody can get infected by the COVID-19, and nobody can escape from the danger of getting tainted by the virus. Yet, the line of hope is that anyone and everyone can boost their resistance, thus avoid the risk of getting affected by the illness. The immunity of humans pulls down as they grow older. If their immune system is robust, them falling sick is feeble. If their resistance is weak, them getting ill is sound. Several factors affect the immune system and its ability, including its nourishment. A two-way connection between nutrition, infection and immunity presents. Changes in one part will affect the others part in our body that's the nature's rule. Well defined immune system quality which is present between each life phase may influence the type, generality and the degree of infections. At the same time, low nutrition to the body will decrease the immune function and expose the body to the danger of getting infected by infectious diseases. Different quantity of micronutrients is required for increasing the immunity power of our body. Generally the vitamins A,C,D,E,B2,B6,B12, iron, zinc and selenium.The deficiencies of micronutrients are acknowledged as a global health issue, and also low nutrition makes it prone to establishes the infections in the body.

Role of Rasayana in Prevention of COVID-19 - A Review

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 716-722
Author(s):  
Sneha Dhakite ◽  
Sadhana Misar Wajpeyi
Keyword(s):  
Immune System ◽  
Respiratory System ◽  
Viral Infections ◽  
Cost Effective ◽  
The Body ◽  
Healthy Life ◽  
Vital Functions ◽  
Healthy Life Style ◽  
And Control

The “Coronavirus disease 19 (COVID-19)” is caused by “Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)”, a newly discovered member of the Coronaviridae family of viruses which is a highly communicable. There is no effective medical treatment till date for Coronavirus disease hence prevention is the best way to keep disease away. Rasayana proved to be highly efficacious and cost effective for the Prevention and Control of viral infections when vaccines and standard therapies are lacking. Rasayana Chikitsa is one of the eight branches of Ashtanga Ayurveda which helps to maintain healthy life style. Rasayana improves immunity and performs many vital functions of human body. Vyadhikshamatva that is immune mechanism of the body is involved in Prevention of the occurrence of a new disease and it also decreases the virulence and progression of an existing disease. In COVID-19 the Respiratory system mainly get affected which is evident from its symptoms like cold, cough and breathlessness. Here the drugs help in enhancing immune system and strengthening functions of Respiratory system can be useful. For this purpose, the Rasayana like Chyavanprasha, Agastya Haritaki, Pippali Rasayana, Guduchi, Yashtimadhu, Haridra, Ashwagandha, Tulsi are used. Rasayana working on Respiratory system are best for Prevention of Coronavirus and boosting immune system. Rasayana Chikitsa can be effective in the Prevention as well as reducing symptoms of COVID-19.

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