scholarly journals The Prevalence of Extended Spectrum Beta-Lactamase (ESBL) Producing Gut Bacterial Flora Among Patients in Dr. Soetomo Hospital and Primary Health Centre in Surabaya

2018 ◽  
Vol 54 (4) ◽  
pp. 256
Author(s):  
Dian Neni Naelasari ◽  
Eko Budi Koendhori ◽  
Linda Dewanti ◽  
Rosantia Sarassari ◽  
K Kuntaman

The extended-spectrum b-lactamase (ESBL) producer bacteria until now were mostly identified in hospital environment. The aim of this study was to analyze the prevalence of ESBL-producing gut flora and distribution of ESBL encoding genes between hospitalized patient in Tropical Wards of Dr. Soetomo Hospital and patient from a primary health centre (PHC) as community environment in Surabaya. Thiry rectal swab samples from hospital of Dr. Soetomo patients and from PHC (60 samples in total) were collected for this study. Samples were screened in MacConkey agar supplemented with 2 mg/L of cefotaxim, incubated at 37ºC for 24 hours. Then the growing colony were confirmed with Disk Diffusion Synergy test (DDST) for diagnosis of ESBL producer. The identified ESBL producers were then identified the bacteria species by biochemical method. ESBL gene were detected by PCR with specific primers. The results showed that there was not difference of positif nuber of ESBL-producing bacteria gut floral between patients of Dr.Soetomo Hospital, 25/30 (83.3%) and PHC, 11/30 (36.7%) (p=1). The pattern of ESBL gene distributions among samples from hospital showed that SHV was 12%, TEM was 36%, and CTX-M was 80%, and from PHC were SHV 18.2%, TEM 27,3% and CTX-M 81,8%. Statistical analysis showed that the pattern was not significantly different among hospitals and PHC samples as shown by SHV gene (p=0,631), TEM (p= 0.715), and CTX -M (p=1). From each ESBL gene, the dominant genes that found producing ESBL were the CTX-M genes followed by TEM and SHV genes. The prevalence of ESBL producersin intestinal flora of both the hospital (83,3%) and the PHC (36,7%) was very high. There was not significant difference between the prevalence of ESBL producer in gut flora of hospitalized patients compared to PHC. There was found other patterns of ESBL gene combinations in the hospital of SHV+CTX-M genes, TEM+CTX-M, SHV+TEM+CTX-M genes and PHC, the combination pattern of SHV+CTX-M, TEM+CTX-M.

2020 ◽  
Vol 151 ◽  
pp. 01018
Author(s):  
Raden A.K. Lestari ◽  
Novyan Lusiyana ◽  
Fitria S. Nurochmah

Kokap is one of the endemic areas of malaria in Yogyakarta. The transmission of malaria was influenced by multiple factors including season. The impact of the rainfall rate in the rainy season was increasing the breeding place of vector-borne diseases, especially malaria. This study aims to describe the case of malaria, the relationship with season and mapping of endemic areas of malaria in Kokap I Primary Health Centre at Kulon Progo in 2012-2017. This is a descriptive epidemiological study with an ecological study approach. The study population was malaria cases in Kokap I Primary Health Centre. Data were analyzed descriptively and then mapped based on the endemic areas. The result showed 245 cases of malaria consisting of 136 men (55.5%) and 109 women (44.5%), on which 166 of them were in 15-45 years age group (67.7%). There were 59.2% of patients were positive of P. vivax infection and 40% of patients were positive of P. falciparum. Malaria cases in 2012-2017 at rainy season, moist season, dry season were 109, 59 and 77 respectively. Malaria cases were increased from October to April which is the rainy season, but there is no significant difference between rainy season and dry season with the incidence of malaria (p=0.316). Kalirejo village was found as a medium-high endemic village, while Hargomulyo and Hargorejo Villages were lowmedium endemic villages. During 2012-2017, we found that malaria in Kokap was dominated with P. vivax infection and the malaria incidence was increased in the rainy season.


Study was carried out in the Kalppara tribal colony under the panchayat of Chelakkara, Thrissur District in order to analyse the health status and to study effect of environment on health. The total population of the tribal colony is 108, 22 families. Survey was conducted in the colony focussing on their health issues. Our study revealed that rather than few common diseases like fever and cold no other major diseases were found in the population which can be attributed to the geographical factors. Primary health centre facility is utilised well by the people and health status is satisfactory in this colony.


2015 ◽  
Vol 3 (1) ◽  
pp. 11-14
Author(s):  
Doddihal Chandrika ◽  
◽  
Metgud Chandra ◽  
Katti Sangappa ◽  
Mallapur Maheshwar ◽  
...  

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