scholarly journals Pengaruh Pemberian Bakteri Bacillus pumilus pada kotoran Sapi Sebagai Pupuk terhadap Jumlah Kandungan Klorofil Dunaliella salina [The Effect of Bacteria Bacillus pumilus In Cow Dung As Fertilizer to Total Chlorophyll Dunaliella salina]

2019 ◽  
Vol 3 (1) ◽  
pp. 53
Author(s):  
Endang Dewi Masithah, Nuansa Adharia Ningrum, Setiawati Sigit
Keyword(s):  
Dunaliella Salina ◽  
Culture Medium ◽  
Bacillus Pumilus ◽  
Natural Food ◽  
Cow Dung ◽  
Chlorophyll B ◽  
Glass Bottle ◽  
Randomized Design ◽  
Fermentation Control ◽  
Completely Randomized Design

AbstractDunaliella salina is one type of natural food used in the hatchery business. Production stability D. salina with an abundance of nutrients can be supported. The purpose of this study was to determine the effect and how many doses of the bacteria Bacillus pumilus best fermented in cow dung as fertilizer in increasing the population of D. salina. Research conducted at the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University Surabaya. The research design used was Completely Randomized Design (CRD) is followed by Duncan test. D. salina was cultured in 250 mL glass bottle with 5 treatments (2 treatments as a control) and four replications. Culture medium used contained 10 ppm cow dung. The concentration of cow manure are given in the study, namely A (12.5% Bacillus pumilus), B (10% Bacillus pumilus), C (7.5% Bacillus pumilus), control 1 (without fermentation), control 2 (fertilizer Walne). The results showed that the addition of Bacillus pumilus on cow dung that were cultured in culture medium D. salina can increase the amount of chlorophyll content of D. salina. Addition of Bacillus pumilus by 10% in the culture medium to produce the amount of chlorophyll-a D. salina high of 0.014055 µg/mL and chlorophyll-b of 0.009657142 µg/mL on the first day. 

Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Denovis Sambode ◽  
Henneke Pangkey ◽  
Sartje Lantu
Keyword(s):  
Population Growth ◽  
Culture Medium ◽  
Quality Parameters ◽  
Organic Fertilizers ◽  
Cow Dung ◽  
Randomized Design ◽  
Period Data ◽  
Growth Development ◽  
Completely Randomized Design ◽  
Medium Culture

The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.

scholarly journals PENGARUH PENCAMPURAN KOTORAN TERNAK SEBAGAI MEDIA KULTUR TERHADAP PERTAMBAHAN POPULASI CACING TANAH (Lumbricus rubellus)

2019 ◽  
Vol 34 (1) ◽  
pp. 75-80
Author(s):  
Satria Edi Putra ◽  
Iskandar Johan ◽  
Muhammad Hasby
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fish Farming ◽  
Lumbricus Rubellus ◽  
Cattle Dung ◽  
Cow Dung ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Buffalo Dung ◽  
Growing Population

This research aims to determine the effect of mixing cattle dung as a culture medium to the growing population of earthworms. The research was conducted for 42 days starting from 18 October to 28 November 2017 at the fish farming school hall of Agriculture Faculty of Islamic University Riau Pekanbaru. The worm tree used in this study was as many as 960 tail or 80 tail for each container. Worms are maintained for 42 days with different mixtures of medium, i,e, 100% P1 dung treatment, P2 cow-dung treatment 50% + 50% buffalo dung, 50% cow dung + 50% goat dung, 50% cow dung + 50% chicken droppings. The research design was Completely Randomized Design with 4 treatments and 3 replications. The result of this research showed that the increase of population of earthworm with the mixing of cattle dung as culture media which conducted for 42 days is the best treatment of P2 as much as 4266 head/container, then the second one used was in treatment 3 as much as 1855 head/container, and the lowest increase of the population was at treatment P1 as much as 403 head/container, and followed by treatment P4 as much as 575 head/ container.

scholarly journals Pemanfaatan Isi Rumen Sapi yang Difermentasikan dengan Bakteri Bacillus pumilus terhadap Kandungan Klorofil pada Kultur Dunaliella salina [Use of Content Cow Rumen Fermented With Bacillus pumilus Bacterial Chlorophyll Content On The Culture Dunaliella salina]

2019 ◽  
Vol 3 (1) ◽  
pp. 97
Author(s):  
Endang Dewi Masithah, Nur Choiriyah, Prayogo
Keyword(s):  
Chlorophyll Content ◽  
Dunaliella Salina ◽  
Bacillus Pumilus ◽  
Sea Water ◽  
Rumen Fluid ◽  
Rumen Bacterium ◽  
Natural Food ◽  
Randomized Design ◽  
Treatment Research ◽  
Bacterium Bacillus

Abstract Dunaliella salina is one type of natural food that can be used in an attempt pembeniha rumen contents of cows is one of the waste obtained from the abattoir which is rich in nutrients. Synthesis of chlorophyll a and b require N and P. The purpose of this study was to determine the effect and the best dose utilization of rumen contents of cows and Bacillus pumilus in the culture of Dunaliella salina in chlorophyll content. The research design used was Completely Randomized Design (CRD) followed by Duncan multiple range test. Treatment research Dunaliella salina was cultured in 500 mL glass bottle with 5 treatments and 4 replications. Culture medium used was sea water that has salinity 30 ppt as much as 0.5 liters of rumen fluid is added 0.5 ml of solution that has been fermented with Bacillus pumilus in accordance with the treatment dose. The results showed that the use of fermenter bacteria Bacillus pumilus in cow rumen contents effect on chlorophyll content and dose fermenter rumen bacterium Bacillus pumilus in cattle that give the best chlorophyll content in Dunaliella salina culture fermentation is the dose intreatment C (7.5 % dose fermentation) reached 0.715653211aìmolchl/cell on the first day. 

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Study on Preparation of Pomegranate Jam Fortified with Extracts of Some Horticultural Plants

2021 ◽  
Vol 10 (9) ◽  
pp. 114-120
Author(s):  
Manideepa Roy Pooja Upasana Bhanj ◽  
Sipra Mohapatra Ankan Das
Keyword(s):  
Statistical Analysis ◽  
Green Tea ◽  
Sodium Benzoate ◽  
Present Experiment ◽  
Glass Bottle ◽  
Randomized Design ◽  
Horticultural Plants ◽  
Completely Randomized Design ◽  
Tea Extract ◽  
Coriander Seed

Pomegranate fruits are very beneficial to us and if some processed thing can be developed from it, further enhanced with extracts of important horticultural plants than it can be additionally more useful to us. Therefore, keeping this thing in mind, the work was undertaken. In the present experiment fortified pomegranate jam was prepared and analyzed. The preparation of jam, by adding extract of beneficial horticultural plants along with specified concentration of pectin and sodium benzoate was done with continuous stirring while heating. The extracts used for fortification of the jam were taken from mint, coriander, green tea, coffee and basil. Overall there were six treatments including the control. After preparation of the fortified pomegranate jam, they were stored in glass bottle in refrigerated condition. All the treatments were replicated three times and Completely Randomized Design was used for statistical analysis. Attributes were studied in the laboratory at different days of storage interval. From the total experiment it was obtained that the pomegranate jam fortified with coriander seed and basil extract were the best which was followed by green tea extract as fortifying agent, as they showed good results.

scholarly journals EFEKTIVITAS PEMBERIAN HARA MIKRO MELALUI MEDIA DAN DAUN PADA TANAMAN KANGKUNG (Ipomea reptans Poir)

Agrin ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 39
Author(s):  
Bhaskara Anggarda Gathot Subrata ◽  
Stefany Darsan
Keyword(s):  
Leaf Area ◽  
Rate Ratio ◽  
Growth Analysis ◽  
Dry Weight ◽  
Growth And Yield ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design

Penelitian bertujuan untuk mengetahui efektivitas pemberian hara mikro melalui akar dan daun. Penelitiandilaksanakan dengan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal. Perlakuan berupa kontrol,pupuk mikro lewat media, pupuk mikro lewat daun. Masing-masing perlakuan diulang 6 kali. Media tanam berupapasir yang sudah dimasukan ke dalam bak penanaman, setelah itu tanam benih kangkung dengan jarak 10x10 cmpada setiap bak. Lalu aplikasikan pupuk melalui media atau daun seminggu sekali. Pengamatan yang dilakukanmeliputi tinggi tanaman dan kehijauan daun dengan SPAD 502 untuk tiap minggunya; aktivitas nitrate reduktase,kandungan klorofil a, klorofil b, dan klorofil total, bobot kering batang, bobot kering tajuk, bobot kering daun,bobot kering akar, luas daun (LD), serta analisis pertumbuhan tanaman, meliputi nisbah laju daun (NLD), luasdaun khas (LDK), bobot daun khas (BDK). Berdasarkan penelitian yang telah dilakukan, pemberian pupuk cairmelalui media maupun daun belum mampu meningkatkan pertumbuhan dan hasil tanaman kangkung. Perlakuanpada daun cenderung menunjukan hasil yang lebih tinggi dibandingkan perlakuan pada media pada semuaparameter pengamatan.Kata kunci: efektivitas, hara mikro, akar dan daunABSTRACTThe aim of this research is to know the effectiveness of micro nutrient giving through root and leaf. Theresearch was conducted by using Completely Randomized Design (CRD) of single factor. Treatment of control,micro-fertilizer through the media, micro-fertilizer through the leaves. Each treatment was repeated 6 times.Planting media in the form of sand that has been inserted into the tub of planting, after that plant the seeds ofkangkung with a distance of 10x10 cm in each tub. Then apply the fertilizer through the media or leaves once aweek. Observations included plant height and greenery of leaves with SPAD 502 for each week; nitrate reductaseactivity, chlorophyll a, chlorophyll b, and total chlorophyll, dry weight of stalk, dry weight of canopy, leaf dryweight, root dry weight, leaf area, and plant growth analysis, including leaf rate ratio, typical leaf area, typicalleaf weight. Based on the research that has been done, the application of liquid fertilizer through the media andleaves has not been to increase the growth and yield of kangkung. Treatment on the leaves tend to show higherresults than treatment on media at all observation parameter.Keywords: effectiveness, micro nutrients, roots and leaves

scholarly journals PHYSIOLOGICAL EFFECTS OF PROFENOFOS INSECTICIDE TOWARDS CONSUMPTION RATE 6 AND ASSIMILATION EFFICIENCY OF COW – DUNG BY EARTHWORMS 7 PONTOSCOLE CORETHRURUS FR. MULL

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Erwin Nofyan ◽  
Mustafa Kamal ◽  
Syafrina Lamin ◽  
Syedzar Al-Ghifari
Keyword(s):  
Data Analysis ◽  
Consumption Rate ◽  
Assimilation Efficiency ◽  
Cow Dung ◽  
Physiological Effects ◽  
Pontoscolex Corethrurus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Research Show ◽  
Rate Of Consumption

The purpose of this research is to determined the effect of insecticide profenofos to the rate of consumption and assimilation efficiency of cow- dung by earthworms Pontoscolex corethrurus Fr. Mull. This research used Completely Randomized Design with 6 treatments and 5 repetition. Treatments that was given to sample are the insecticide profenofos with concentration of 0 % (control); 0.1%; 0.2 %; 0.3 %; 0.4 %; 0.5 %. Data analysis was using Varians Analysis. If there was real difference then data analysis continued with The Duncan Test on level of confidence of 95%. The results of this research show us that several concentration of insecticide carbaryl have the real effect to the average of consumption rate and the efficiency of absorption. The lowest average of consumption rate on earthworm Pontoscolex corethrurus Fr.Mull is on concentration of 0.5 % (0.23 ± 0.02 mg/g day) and the highest average of consumption 26 rate on earthworm Pontoscolex corethrurus Fr. Mull is on concentration of 0% (control) (2.53 ± 0.05 mg/g day). The lowest average of approach digestyon earthworm Pontoscolex corethrurus is on concentration of 0 % (control) (40.78 28 ± 2.56 %) and the highest average of digestion earth worm Pontoscolex corethrurus Fr.Mull is on concentration of 0.5 % 29 (70.76 ± 3.67 %).

scholarly journals Investigated of Desalination of Saline Waters by Using Dunaliella Salina Algae and Its Effect on Water Ions

2019 ◽  
Vol 5 (11) ◽  
pp. 2450-2460 ◽  
Author(s):  
Asma Moayedi ◽  
Bahman Yargholi ◽  
Ebrahim Pazira ◽  
Hossein Babazadeh
Keyword(s):  
Dunaliella Salina ◽  
Saline Water ◽  
Absorption Process ◽  
Human Needs ◽  
Salt Absorption ◽  
Good Ability ◽  
Randomized Design ◽  
Saline Waters ◽  
Completely Randomized Design ◽  
Biological Methods

Nowadays, due to augmentation of population and expansion of cities and the limitation of fresh water sources in the world, existing water resources cannot meet the human needs. Desalination or reduction of salinity of water through biological methods involves the use of plant species, microorganisms, algae or a combination of them, which can be effective in reducing water salinity. The objective of this study was to investigate the desalination of saline waters by using Dunaliella salina algae. For this purpose, the experiments were carried out as factorial based on completely randomized design. The expected tests were conducted in a laboratory controlled condition for humidity, light and temperature (90 days). During this research, the Electrical Conductivity (EC) was measured daily. The results showed that salt absorption in Dunaliella salina algae was significantly different. High salt absorption was observed in Dunaliella salina at a concentration of 130mS.cm-1. Due to the constant humidity, light and temperature, salt removal from saline water was observed in laboratory conditions. The results of this study indicate that significant reduction of chlorine, sodium and bicarbonate levels were observed using Dunaliella salina algae. According to the results of this study, it seems that reducing salt absorption in algae is due to the use of salt in the metabolism and the growth and proliferation of algae. The absorption process in this study showed that the catch of Dunaliella salina has a good ability to remove salt and can be used as an appropriate suggestion for salt removal from saline water.

scholarly journals Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical

2011 ◽  
Vol 21 (4) ◽  
pp. 293 ◽  
Author(s):  
Budi Winarto
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Formation ◽  
Potential Growth ◽  
Randomized Design ◽  
Ornamental Crops ◽  
Completely Randomized Design ◽  
Anthurium Andraeanum ◽  
Positive Effect

Kultur anter merupakan salah satu teknologi haploid penting dalam produksi tanaman haploid ganda dan berhasil diaplikasikan pada berbagai jenis tanaman, namun aplikasi pada Anthurium belum pernah dilaporkan. Penelitian dan pengembangan kultur anter Anthurium yang difokuskan untuk mempelajari pengaruh glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias dari bulan Januari sampai dengan September 2008. Tujuan penelitian ialah mengetahui pengaruh kombinasi konsentrasi glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus pada kultur anter Anthurium. Spadik Anthurium andraeanum cv. Tropical, kalus hasil kultur anter serta medium Winarto dan Teixeira digunakan dalam studi ini. Glutamin dan serin pada konsentrasi 0, 250, 500, dan 750 mg/l diuji dalam percobaan ini. Percobaan disusun menggunakan rancangan acak lengkap pola faktorial dengan empat ulangan. Hasil studi menunjukkan bahwa penambahan glutamin dan serin pada medium terseleksi belum memberikan pengaruh yang signifikan terhadap induksi, pertumbuhan, dan regenerasi kalus. Glutamin pada konsentrasi 250 mg/l menginduksi potensi tumbuh anter hingga 48% dengan 21% anter beregenerasi dan 1,3 anter per perlakuan membentuk kalus. Sementara serin pada 500 mg/l merupakan konsentrasi yang paling potensial dalam induksi kalus dengan 55% potensi tumbuh anter, 24% anter beregenerasi, dan 1,4 anter per perlakuan membentuk kalus. Glutamin 250 mg/l merupakan konsentrasi terbaik dibanding konsentrasi yang lain dalam mendukung pertumbuhan dan regenerasi kalus. Perlakuan tersebut tanpa serin mampu menginduksi potensi pertumbuhan kalus hingga 77% dengan volume kalus mencapai 237 mm3 dan empat tunas dihasilkan per eksplan. Sementara perlakuan serin justru mereduksi pertumbuhan dan regenerasi kalus dan menstimulasi senesensi kalus yang berdampak pada pencoklatan dan kematiannya. Dari hasil penelitian ini dapat disarankan penggunaan glutamin dibanding serin dalam meningkatkan keberhasilan kultur anter Anthurium.<br /><br /><br /><br />Anther culture is one of important haploid technologies in producing double haploid lines and successfully applied in many plants, while the application in Anthurium is not reported yet. Research and development in anther culture of Anthurium focusing on studying the effect of glutamine and serine on callus induction, growth, and its regeneration was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute from January untill September 2008. Objective of this study was to know the effect of glutamine and serine on callus induction, growth, and its regeneration in anther culture of Anthurium. Spadix of Anthurium andraeanum cv. Tropical, callus derived from anther and Winarto and Teixeira medium were utilized in the study. Glutamine and serine of 0, 250, 500, and 750 mg/l were tested in the experiments. Factorial experiment was arranged by completely randomized design with four replications. Results of the study indicate that addition of glutamine and serine in selected culture medium gave moderate significant effect on induction, growth, and regeneration of callus. Glutamine in 250 mg/l induced potential growth of anther up to 48% with 21% regenerated anthers and 1.3 anthers per treatment producing calli, while 500 mg/l of serine was better concentration in callus formation with 55% potential growth of callus, 24% regenerated anthers and 1.4 anthers per treatment producing calli. In growth and regeneration of callus, supplementation of serine reduced callus capacity in growth and production of shoots and stimulated callus senescence causing browning and death of it, while 250 mg/l glutamine exhibited positive effect on them. The treatment without serine was able to induce potential growth of callus up to 77% with 237 mm3 per callus and four shoots produced per explants. Results of the study suggest application of glutamine rather than serine in improving anther culture of Anthurium.<br /><br />

scholarly journals Effect of nitrogen fertilizer rates on growth and oil yield of Java lemongrass (Cymbopogon winterianus Jawitt) in Thu Duc, Ho Chi Minh City

2021 ◽  
Vol 20 (1) ◽  
pp. 10-16
Author(s):  
Lan P. H. Nguyen
Keyword(s):  
Nitrogen Fertilizer ◽  
Oil Yield ◽  
Ho Chi Minh City ◽  
Average Weight ◽  
Cow Dung ◽  
Randomized Design ◽  
Leaf Yield ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Fertilizer Rates

Java lemongrass is commonly cultivated in Vietnam for the use in industries and traditional medicine. Currently, the application of fertilizer, especially nitrogen fertilizer, is one of the most traditional farm practices to increase production yield. A single factorial experiment was carried out in a completely randomized design, three replications to determine the suitability of nitrogen fertilizer rates on growth, leaf and oil yields of Java lemongrass. Six treatments were used in this experiment including 90, 120, 150, 180, 210, 240 kg N/ha and applying 90 kg N/ha as control. Fertilizer base for treatments (for 1 ha) included 20 tons of cow dung compost, 60 kg P2O5 and 60 kg K2O. The application of 120 kg N/ha significantly improved the growth of Java lemongrass as plant height (97.0 cm/plant), average number of leaves (197.1 leaves/plant), average weight of leaves per plant (198.7 g/plant), fully leaf yield (9.4 tons/ha/2 harvest times), essential oil content (1.0% FW) and economic oil yield (97.8 kg/ha/2 harvests).

Sign in / Sign up

Export Citation Format

Share Document