scholarly journals Calculation Of Coliform Number Using Most Probable Number (MPN) Methods On Soy Milk Sold in Pogot Area Of Surabaya

2020 ◽  
Vol 4 (1) ◽  
pp. 26
Author(s):  
Racma Nisaa
Keyword(s):  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Descriptive Study ◽  
National Standard ◽  
Most Probable Number ◽  
Soy Milk ◽  
Street Vendors ◽  
Probable Number ◽  
Maximum Limit

The quality of drinks is one of the problems that exist in society, especially because of bacterial contamination of drinks that often occurs in developing countries. One of them is soy milk drinks that are contaminated by bacteria including those sold by street vendors in the Pogot area of Surabaya. Based on the background description in this study can be formulated as follows: Is the number of Most Probable Number (MPN) Coliform in soy milk sold by street vendors in the Pogot area of Surabaya fulfill the Indonesian National Standard (SNI) 7388: 2009? The purpose of this study was to determine the amount of Coliform in soy milk sold by street vendors in the Pogot area of Surabaya compared to the Indonesian National Standard (SNI) 7388: 2009 regarding the maximum limit of microbial contamination in food. This study used a descriptive study design that soy milk sold by street vendors in the Pogot area of Surabaya was the population. Samples were used as many as 16 samples from 4 street vendors, each of which was taken 4 then laboratory-tested and analyzed using percentages. Based on the requirements of the Indonesian National Standard (SNI) 7388: 2009, the limit of contamination of the Most Probable Number (MPN) Coliform in soy milk is 20 / ml. Based on the results of the research of 4 soy milk traders there were 2 traders whose the results fulfilled the requirements of the Indonesian National Standard (SNI) and 2 traders whose the results didn't  fulfill the requirements of the Indonesian National Standard (SNI). So 50% were eligible (MS) and 50% were not eligible (TMS).

scholarly journals Uji Angka Lempeng Total dan Kontaminan Koliform pada Susu Kedelai Bermerek yang Beredar di Kota Denpasar

2021 ◽  
Vol 9 (1) ◽  
pp. 141
Author(s):  
Michael Jordi Theofanny ◽  
Ida Bagus Wayan Gunam ◽  
Ni Putu Suwariani
Keyword(s):  
National Standard ◽  
Plate Count ◽  
Most Probable Number ◽  
Soy Milk ◽  
Probable Number ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Sample Testing ◽  
Good For ◽  
Legal Product

This research aimed to testing total plate count and coliform contaminant on legal product soy milk sold in Denpasar city is qualified to Indonesian National Standard (SNI) and safe to consumption. Soy milk has high nutrition and good for growth microorganisms. Microorganisms in soy milk is good for health but be worried have a dangerous microorganisms. Samples of soy milk based on purposive method, tested with total plate count agar with pour plate method and contaminant coliform with most probable number method. The result of testing total plate count, all samples is under maximum of SNI  5 × 104CFU/g. After that soy milk sample testing of coliform contaminant, the result is all samples has negative coliform contaminant. Conclusion of the research is all of the legal product soy milk sold in Denpasar qualified to SNI No. 01-3830-1995 and safe to consumption. Keyword: coliform, Denpasar city, Indonesian national standard, soy milk, total plate count.

scholarly journals Identifikasi Bakteri Escherichia coli Susu Kedelai Murni di Pasar Jodoh Kota Batam

2021 ◽  
Vol 7 (1) ◽  
pp. 25-30
Author(s):  
Sri Hainil ◽  
Trie Yuni Elfasyari ◽  
Rofina Intan Sulistya
Keyword(s):  
Escherichia Coli ◽  
The Body ◽  
National Standard ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Soy Milk ◽  
Normal Flora ◽  
Healthy Human ◽  
Probable Number ◽  
Escherichia Coli Bacteria

Pure soy milk is a highly nutritious drink; in general, pure soy milk contains excellent content for the body in the form of protein, iron, phosphorus, carbohydrates, fat, provitamin A and B vitamins. Causing pure soy milk to contain or be contaminated with microorganisms. one of the microorganisms found in drinks or water is the Coliform bacteria. An example of Coliform bacteria is Escherichia coli. Escherichia coli is a bacterium that lives in the human intestine; these bacteria live as normal flora or can be called a collection of microorganisms, naturally present in normal and healthy human bodies. The research objective was to determine the presence or absence of Escherichia coli in pure soy milk sold in Jodoh Market, Batam City. This study uses the Most Probable Number (MPN) method. The results of the study on 10 samples of pure soy milk A, B, C, D, E, F, G, H, I, J and 2 samples of soy milk branded K, and L contained coliform bacteria, and there were 6 Escherichia coli bacteria in soy milk samples. Pure A, B, C, D, G, and on samples of soy milk branded K. The results of the examination of 12 samples of soy milk were found not to meet the requirements of the Indonesian National Standard (INS 01 – 3830 – 1995).

scholarly journals Assessment of microbial quality of household water output from desalination systems by the heterotrophic plate count method

2018 ◽  
Vol 16 (6) ◽  
pp. 930-937 ◽  
Author(s):  
Ahmad Reza Yari ◽  
Mohammad Javad Mohammadi ◽  
Sahar Geravandi ◽  
Zohreh Doosti ◽  
Soudabeh Alizadeh Matboo ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
National Standard ◽  
Plate Count ◽  
Most Probable Number ◽  
Microbial Quality ◽  
Heterotrophic Plate Count ◽  
Probable Number ◽  
Household Water ◽  
Standard Values

Abstract Point-of-use household water desalination systems (HWDSs) are becoming popular in Iran because of the deterioration of drinking water. This study aimed to determine the microbial quality of output water from HWDSs in Qom, Iran by using the heterotrophic plate count (HPC) method. Samples of input and output water from 30 HWDSs were collected over a six-month period. Heterotrophic bacteria were tested using the pour plate technique. At the first sampling stage, the HPC level in 23% of samples exceeded the 500 CFU/ml threshold level. On average, for 50% of samples, the HPC level of input samples was 0–10 CFU/ml, for 42% it was 10–100 CFU/ml and for 8% it was 100–500 CFU/ml. For output samples, for 25%, the level of HPC was 0–10 CFU/ml, for 43% it was 10–100 CFU/ml, for 24% it was 100–500 CFU/ml and for 8% it exceeded 500 CFU/ml. For total coliforms the most probable number test was positive for the first and third stages of sampling (3% input samples). The comparison of the averages with national standard values shows that in some cases, the contamination of output water from HWDSs in the city of Qom has been above the standard values.

scholarly journals Microbiological counting in lamb carcasses from an abattoir in São Paulo, Brazil

2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Sao Paulo ◽  
Most Probable Number ◽  
São Paulo ◽  
Probable Number ◽  
Lamb Meat ◽  
Thermotolerant Coliforms ◽  
Sanitary Conditions

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.

scholarly journals Quality of water sources in Southwestern Uganda using the compartment bag test (CBT): a cross-sectional descriptive study

2019 ◽  
Vol 9 (4) ◽  
pp. 683-693
Author(s):  
Richard Onyuthi Apecu ◽  
Lucas Ampaire ◽  
Edgar Mugema Mulogo ◽  
Fred Norman Bagenda ◽  
Afsatou Traore ◽  
...  
Keyword(s):  
Water Samples ◽  
Water Sources ◽  
World Health ◽  
Most Probable Number ◽  
Physical Parameters ◽  
Probable Number ◽  
Gravitational Flow ◽  
Quality Of Water ◽  
Compartment Bag Test

Abstract The aim of this study was to assess the bacteriological quality of water sources in the two rural areas of Uganda using the compartment bag test (CBT). In total, 200 water samples were collected from 69 different water sources and processed within 6 h of collection. Positive and negative controls were processed each day together with water samples. Physical parameters were measured in situ. Descriptive statistics were used to generate mean, minimum, maximum, standard deviations and percentages. The results indicated that 29% of the water sources met the National Standards and World Health Organization (WHO) Guidelines for drinking water. Sixty percent of the borehole, 44% of gravitational flow taps and 14% of roof rain water met the required standards. Of the open water sources, 75% of the rivers, 50% of open channels and 43% of unprotected dug wells plus 25% of protected springs and 9% of gravitational flow schemes had most probable number counts &gt;100 Escherichia coli/100 mL of water. Most of the water sources in the study areas were not fit for human consumption without prior treatment. The CBT was found to be robust and easy to use in all field situations. The mean physical parameters of water sources were within the acceptable limits.

scholarly journals Physiochemical and Bacteriological Analysis of Selected Sachet Water in Jere and Maiduguri, Borno State Nigeria

2015 ◽  
Vol 4 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Mohammed Modu Aji ◽  
Shettima Abba Kyar ◽  
Mustapha Hussaini
Keyword(s):  
Total Alkalinity ◽  
World Health ◽  
Most Probable Number ◽  
Expiry Date ◽  
Probable Number ◽  
Bacteriological Analysis ◽  
Batch Number ◽  
Registration Number ◽  
Sachet Water

 The study involved the determination of some physiochemical and bacteriological properties in sachet-water samples taken within selected geopolitical wards in Jere and Maiduguri Metropolis. The purpose was to ascertain the quality of sachet water sold for consumption within the area. Ten samples were drawn, five from each study area. The samples were analysed for temperature, colour, turbidity, pH, conductivity, iron, total alkalinity and total dissolved solid. Bacteriological analysis was also carried out using multiple tube (most probable number) technique for enumeration of both total coli form count and differential Escherichia coli count. Other physical examination like volume, National Agency for Food and Drugs Administration and Control (NAFDAC) registration number, batch number, production date and expiry date were also examined. The results obtained were compared with World Health Organization (WHO), NAFDAC and Nigeria Standard of Drinking Water Quality (NSDWQ). Variations were observed among the entire samples in comparison with the standard. Some of the parameters conform to the standard like the Tempreture, pH, colour, odour, iron content and NAFDAC registration number while others like TDS, total alkalinity and volumetric quantity fell below the standard. The bacteriological analysis also showed that 80% of the samples studied revealed the presence of coliforms. Hence, there is need for regulatory agencies like NAFDAC and Standard Organisation of Nigeria (SON) to intensify effort in the routine monitoring of quality of sachet water marketed for consumption.DOI: http://dx.doi.org/10.3126/ije.v4i1.12177 International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, page: 48-57  

scholarly journals Managing pollutant inputs from pastoral dairy farming to maintain water quality of a lake in a high-rainfall catchment

2013 ◽  
Vol 64 (5) ◽  
pp. 447 ◽  
Author(s):  
Robert J. Wilcock ◽  
Ross M. Monaghan ◽  
Richard W. McDowell ◽  
Piet Verburg ◽  
Jonny Horrox ◽  
...  
Keyword(s):  
Stream Water ◽  
Most Probable Number ◽  
Deep Drainage ◽  
Probable Number ◽  
E Coli ◽  
High Rainfall ◽  
Flow Monitoring ◽  
Storm Flow ◽  
Outlet Flow

A study (2004–11) of a dairy catchment stream entering an oligotrophic lake in an area of very high rainfall (~5 m year–1) yielded median concentrations of total nitrogen (TN), total phosphorus (TP), suspended sediment (SS) and Escherichia coli (E. coli) of 0.584, 0.074 and 3.7 g m–3, and 405/100 mL (most probable number method), respectively. Trend analysis indicated significant (P < 0.01) decreases for TN (–0.08 ± 0.02 g m–3 year–1), TP (–0.01 ± 0.005 g m–3 year–1) and SS (–0.45 ± 0.14 g m–3 year–1) and were partly attributable to improved exclusion of cattle from the stream. Water balance calculations indicated that approximately one-half the rainfall left as deep drainage that by-passed catchment outlet flow recorders. Estimates of catchment yields for TN were improved by taking into account groundwater hydrology and concentrations from well samples. Storm-flow monitoring inflows exceeding the 97.5th percentile contributed ~40% of total loads leaving the catchment so that specific yields for SS, TN and TP augmented by groundwater inputs and storm flows were ~960, 45 and 7 kg ha–1 year–1, respectively. These compared well with modelled results for losses from dairy farms in the catchment of 40–60 kg N ha–1 year–1 and 5–6 kg P ha–1 year–1 and indicated that attenuation losses were relatively small.

scholarly journals Comparison of High Hydrostatic Pressure, Ultrasound, and Heat Treatments on the Quality of Strawberry–Apple–Lemon Juice Blend

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 218 ◽  
Author(s):  
Xiaoping Feng ◽  
Zhongyu Zhou ◽  
Xiaoqiong Wang ◽  
Xiufang Bi ◽  
Yuan Ma ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Lemon Juice ◽  
Aerobic Bacteria ◽  
Most Probable Number ◽  
Total Phenols ◽  
Probable Number ◽  
The Us ◽  
Total Anthocyanins

Changes in the microbial, physicochemical, and sensory properties of blended strawberry–apple–lemon juice were investigated to comparatively assess the influence of three processing treatments, namely high hydrostatic pressure (HHP) (500 MPa/15 min/20 °C), ultrasound (US) (376 W/10 min/35 °C), and heat treatment (HT) (86 °C/1 min) over 12 days of storage at 4 °C. The results showed that the total aerobic bacteria (TAB) counts in the HHP-, US-, and HT-treated juice blends were less than 2 log10 CFU/mL, the yeast and mold (Y & M) counts were less than 1.3 log10 CFU/mL, and the coliforms most probable number (MPN/100 mL) was less than 3 after 10 days at 4 °C. Anthocyanins were maintained by HHP, but decreased by 16% and 12% after US and HT, respectively. Total phenols increased by 18% and 7% after HHP and US, respectively, while they were maintained by the HT. Furthermore, better maintenance of total phenols, total anthocyanins, ascorbic acid, antioxidant capacity, color, and sensory values were observed in the HHP-treated juice blend stored for 10 days at 4 °C, compared to both the US- and HT-treated samples. Therefore, HHP was proposed to be a better processing technology for juice blend.

Survival of Escherichia coli on Lettuce under Field Conditions Encountered in the Northeastern United States

2017 ◽  
Vol 80 (7) ◽  
pp. 1214-1221 ◽  
Author(s):  
Daniel L. Weller ◽  
Jasna Kovac ◽  
Sherry Roof ◽  
David J. Kent ◽  
Jeffrey I. Tokman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbial Contamination ◽  
Field Conditions ◽  
Risk Assessments ◽  
Most Probable Number ◽  
Northeastern United States ◽  
Probable Number ◽  
E Coli ◽  
The Relationship ◽  
Sample Time

ABSTRACT Although wildlife intrusion and untreated manure have been associated with microbial contamination of produce, relatively few studies have examined the survival of Escherichia coli on produce under field conditions following contamination (e.g., via splash from wildlife feces). This experimental study was performed to estimate the die-off rate of E. coli on preharvest lettuce following contamination with a fecal slurry. During August 2015, field-grown lettuce was inoculated via pipette with a fecal slurry that was spiked with a three-strain cocktail of rifampin-resistant nonpathogenic E. coli. Ten lettuce heads were harvested at each of 13 time points following inoculation (0, 2.5, 5, and 24 h after inoculation and every 24 h thereafter until day 10). The most probable number (MPN) of E. coli on each lettuce head was determined, and die-off rates were estimated. The relationship between sample time and the log MPN of E. coli per head was modeled using a segmented linear model. This model had a breakpoint at 106 h (95% confidence interval = 69, 142 h) after inoculation, with a daily decrease of 0.70 and 0.19 log MPN for 0 to 106 h and 106 to 240 h following inoculation, respectively. These findings are consistent with die-off rates obtained in similar studies that assessed E. coli survival on produce following irrigation. Overall, these findings provide die-off rates for E. coli on lettuce that can be used in future quantitative risk assessments.

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

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