scholarly journals FORMULATION OF CREAM BODY SCRUB FROM ETHANOL EXTRACT OF CASSAVA LEAVES (Manihot esculenta) AS ANTIOXIDANT

2020 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Fadhliyah Malik ◽  
Suryani Suryani ◽  
Sunandar Ihsan ◽  
Elvianti Meilany ◽  
Rini Hamsidi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Manihot Esculenta ◽  
Test Preparation ◽  
Ethanol Extract ◽  
Cassava Leaves ◽  
Ethanol Determination ◽  
Activity Method ◽  
Free Radical Activity

Background : Plant cassava leaves (Manihot esculenta) are known flavonoid is efficacious as antioxidant. Antioxidant can prevent damage to the skin by reducing free radical activity. Purpose : The purpose of this study to determine the concentration of ethanol extract of leaves of cassava can be formulated into dosage cream body scrub which have antioxidant activity. Method : Cassava leaf extract obtained by maceration using 97% ethanol. Determination of antioxidant activity by the method of reduction of free radical DPPH (1,1-diphenyl-2-picryl-hydrazyl). Result : The ethanol extract of cassava leaves are formulated into dosage cream body scrub with concentration variations 0,0085 mg/mL  (formula 1), 0,017 mg/mL  (formula 2), and 0,0255 mg/mL  (formula 3). Stability test preparation cream body scrub using cycling tests performed for 6 cycles. Preparation cream body scrub to formula I has a moderate antioxidant activity with IC50 158,16 µg/mL, formula 2 has an active antioxidant activity with IC50 66,59 µg/mL, and formula 3 has a stronger antioxidant activity with IC50 38,80 µg/mL. Conclusion : cream body scrub and cassava ethanol extract does not irritate so it is safe to use. Preparations cream body scrub well have the most activity at a concentration of 0,0255 mg/mL with IC50 38,80 µg/mL.

scholarly journals EVALUASI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL SPONS Aplysina sp. DARI PERAIRAN SELAT LEMBEH KOTA BITUNG

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 127
Author(s):  
Aprilia Manumpil ◽  
Defny S Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Coral Reefs ◽  
Ethanol Extract ◽  
Land Plants ◽  
Antioxidant Compounds ◽  
Marine Biota ◽  
Lembeh Strait ◽  
Concentration Test ◽  
Free Radical Activity

ABSTRACTAplysina sp. Sponge is one of the marine biota making up coral reefs that contain active compounds whose percentage of activity is greater than the compounds produced by land plants. This study aims to determine the activity of antioxidant compounds from the ethanol extract of Aplysina sp. from Lembeh Strait Waters, Bitung City. Sponge Aplysina sp., was extracted by maceration with ethanol as a solvent. As a parameter, testing of antioxidant activity was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, which was measured using UV-Vis Spectrophotometry at a wavelength of 517 nm. The result showed the ethanol extract of Aplysina sp., sponge proven to have antioxidant activity in each concentration test. The highest concentration has an antidote to free radical activity by reaching a percentage of 46,13%. Keywords: Aplysina sp. Sponge, Antioxidant, Extraction, DPPH ABSTRAKSpons Aplysina sp. merupakan salah satu biota laut penyusun terumbu karang yang mengandung senyawa aktif yang presentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol Spons Aplysina sp. dari Perairan Selat Lembeh, Kota Bitung. Spons Aplysina sp. diekstraksi menggunakan metode maserasi dengan etanol sebagai pelarut. Sebagai parameter, pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil) yang diukur menggunakan Spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil penelitian menunjukkan ekstrak etanol spons Aplysina sp. terbukti memiliki aktivitas antioksidan disetiap konsentrasi pengujian.Konsentrasi tertinggi memiliki aktivitas penangkal radikal bebas dengan mencapai presentase 46,13%. Kata Kunci : Spons Aplysina sp, Antioksidan, Ekstraksi, DPPH

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

2021 ◽  
Vol 5 (4) ◽  
pp. 365-368
Author(s):  
Muthmainah Tuldjanah ◽  
Niluh Puspita Dewi ◽  
Dian Rahmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
Morinda Citrifolia ◽  
Absorption Method ◽  
Immersion Method ◽  
Activity Test ◽  
Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

scholarly journals Antioxidant Activity of Indian Propolis - An In Vitro Evaluation

2016 ◽  
Vol 5 ◽  
pp. 79-85 ◽  
Author(s):  
Shubharani Ramnath ◽  
Sivaram Venkataramegowda
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Activity Level ◽  
In Vitro Assays ◽  
Antioxidant Compounds

Antioxidants from the natural products are essential to prevent the progression of free radical mediated diseases. In the present study, ethanol extract of propolis collected from different geographical origin were evaluated for their free radical scavenging potential by employing different in-vitro assays such as DPPH, ABTS, Nitric oxide and Hydrogen peroxide. All the tested samples contained considerable amount of total phenols and vitamin C content. In the entire assay, the percentage of inhibition increased with the increase in concentration. Among the propolis samples collected, the highest activity was found in Tamil Nadu, Kerala, Karnataka and Haryana. The difference in the antioxidant activity level was obtained from the assay may reflect a relative difference in the ability of antioxidant compounds to scavenge different free radicals in the extract. Phenols and vitamin C are the major contributors to antioxidant activity in propolis. The propolis from these locations may be of considerable interest in preventing the ill effects of excessive free radical generation in the human body.

scholarly journals EVALUASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 119-128
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals UJI AKTIVITAS SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 129-134
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract   Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals The bioactive contents and antioxidant activity of honey bee nest extract of Apis dorsata Binghami from the North Sulawesi

Molekul ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 92
Author(s):  
Mokosuli Yermia Semuel ◽  
Eva Sherly Nonke Kaunang ◽  
Jacklin Stella Manopo
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Honey Bee ◽  
Ethanol Extract ◽  
Control Treatment ◽  
Apis Dorsata ◽  
Reduction Activity ◽  
North Sulawesi ◽  
Radical Reduction ◽  
Bioactive Content

Apis dorsata Binghami is a Sulawesi endemic honey bee. Apis dorsata Binghami cannot be bred, so it still lives wildlyin the forests of Sulawesi. However, Apis dorsata Binghami produces more honey, compared to all honey bee species. Furthermore, the diversity of plants as a source of nectar, pollen and plant resin, which is used in the formation of nests and honey is more, than all types of honey bees in the world. Ethnomedically, the Minahasa community has long used honeynest for degenerative diseases such as hyperlipidemia and cancer. Nevertheless, there have been no research reports on bioactive content and bioactivity of Apis dorsataBinghami nest extract.  This research wasaimed to determinethe bioactive content of honey bee nest and to obtain the inhibitory concentration 50 (IC50) antioxidant activity of honey bee nest extractofApis dorsata Binghami. Honey bee nest was obtained directly from the forest of Minahasa peninsula, North Sulawesi, Indonesia. Extraction of fresh honey bee nest was conductedusingmaceration method. Bioactive content analysis was carried out by the Harborne method, followed by analysis using UV Vis spectrophotometer and High performance liquid chromatography. IC50antioxidant activity of honey bee nest extract was obtained using DPPH free radical reduction method. The results showed that Apis dorsata Binghami honey bee nest extract containedalkaloids, flavonoids, saponins, tannins, steroids and triterpenoids. Identified flavonoids displayed the highest phytochemical content. Based on the results of HPLC and UV Vis spectrophotometer analysis, there were 20 flavonoid derivatives found in honey bee nest samples in Minahasa. Ethanol extract and n-hexane extract showed high free radical reduction activity compared to vitamin C as a control treatment. However, ethanol extract produced the highest DPPH free radical reduction activity.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

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