scholarly journals PROFIL DAN ANALISIS AKTIVITAS ANTIOKSIDAN DALAM EKSTRAK AIR MENIRAN YANG DIKERINGKAN DENGAN METODE YANG BERBEDA Profile and Analysis of Antioxidant Activity in Dried Meniran Water Extracts using Different Methods

2021 ◽  
Vol 16 (2) ◽  
pp. 150
Author(s):  
Ai Sri Kosnayani ◽  
Liah Badriah ◽  
Asep Kurnia Hidayat ◽  
Muhammad Eka Asri Rizal
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Room Temperature ◽  
Soxhlet Extraction ◽  
Sun Exposure ◽  
Extraction Process ◽  
Sun Drying ◽  
Water Infusion ◽  
Obesity Status ◽  
Color Visualization

Meniran (Phyllanthus niruri Linn.) is a medicinal plant that can reduce obesity status, hypoglycemic, hypotensive, and have antioxidant activity. Meniran has been long used as a medicinal plant, but its utilization in a form of water infusion is still rare. This research is a continuation study which aims to identify the biomolecules that have antioxidant activity in water infusion of meniran. The making of water infusion of meniran requires drying process, which can be done by room temperature drying and sun drying. Phenol and fl avonoid compounds in meniran are assumed to have antioxidant activity. Both compounds are easily oxidized and isomerized due to sun exposure. It is assumed that the drying method will aff ect the presence of phenol and fl avonoid compounds and its antioxidant activity. The study began with the process of sun drying and room temperature without direct sun exposure. Then the extraction process used water soxhlet by soxhlet extraction method. The extract was then tested qualitatively using the DPPH IC50 method. The results of the qualitative analysis with meniran color visualization are positive containing fl avonoids and phenols. The results of quantitative analysis of meniran which are dried by sun drying; fl avonoids 0.90% w/w and 1.65% w/w phenols, in samples stored at room temperature: 2.00% w/w fl avonoids and phenol 56.16% w/w. The antioxidant activity of IC50-DPHH in extract concentrations (2, 4, 6, 8, 10, 15, 20 ppm) of dried meniran at room temperature 18.48 ppm, sun drying cannot be determined.

scholarly journals Effective Utilization of Pineapple Waste, Evaluation of Ferulic Acid, Micro Nutrients and Antioxidant Property

2020 ◽  
Vol 2 (1) ◽  
pp. 30
Keyword(s):  
Antioxidant Activity ◽  
Ferulic Acid ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Antioxidant Property ◽  
Extraction Process ◽  
Fruit Peel ◽  
Manganese Zinc ◽  
Pineapple Peel ◽  
Phosphorus Iron

India is the second-largest producer of fruit and vegetable producers. Usually, after fruit intake, fruit peel is left as waste. The main bioactive components of pineapple are phenolic compounds, β-carotene, ascorbic acid, and flavonoids. Ferulic acid is a phenolic acid widely used in the nutritional and cosmetic fields. In this study, pineapple peel was dried, powdered, and vitamin content (A, B, B1, B2, B6, B12 & C), calcium, potassium, phosphorus, iron, manganese, zinc and food fiber were analyzed. Moreover, in the present study, traditional and non-conventional processes such as Soxhlet extraction, supreme fluid extraction, and normal solvent extraction was used for the extraction of ferulic acid, which is a precursor for vanillin synthesis. The quantification of ferulic acid was done by High performance fluid chromatographic (HPL C) method. After the above-mentioned extraction process overall phenolic and antioxidant activity were also evaluated and compared. The highest concentrations of ferulic acid (0.7696g/100 g), phenolic compound (2.365mg / GAE), antioxidant activity (45 percent), and yield (90.5 %) were obtained for Soxhlet extraction using methanol.

scholarly journals Ionic Liquid–Ultrasound-Based Extraction of Biflavonoids from Selaginella helvetica and Investigation of Their Antioxidant Activity

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3284 ◽  
Author(s):  
Yongmei Jiang ◽  
Dan Li ◽  
Xiankui Ma ◽  
Fengqin Jiang ◽  
Qun He ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Extraction Process ◽  
Ferrous Ions ◽  
Ultrasound Assisted Extraction ◽  
Green Extraction ◽  
Reducing Power Assay ◽  
Free Radical Scavenging Assay

As a new and green solvent, ionic liquids (ILs) have received more attention during the green extraction and separation process for natural medicines. In this paper, IL-ultrasound-assisted extraction (IL-UAE) of total biflavonoids (TBFs) from Selaginella helvetica was firstly developed, and different ILs were employed and compared. Based on single-factor experiment, solid–liquid ratio (1:10–1:14 g/mL), IL concentration (0.6–1.0 mmol/mL), and extract temperature (40–60 °C) were further explored, according to response surface methodology (RSM), with TBF yields as the index. Moreover, antioxidant activity of TBF extract was analyzed by four methods, i.e., 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzth-iazoline-6-sulphonate (ABTS) free radical scavenging assay, ferric ion reducing power assay, and chelation of ferrous ions assay. The results indicated that [C6mim]PF6 had a high selectivity and efficiency. Moreover, important parameters for the extraction process were investigated and optimized. Through parameter optimization (0.8 mmol/L, 250 W, 40 min, 1:12.7 g/mL, and 47 °C), a yield of 18.69 mg/g biflavonoids was obtained from the extract of S. helvetica. Compared with ethanol-UAE, heat-reflux extraction, Soxhlet extraction, and percolation extraction, IL-UAE could not only obtain higher yield in a shorter time, but also reduce the solvent consumption. In addition, TBF extract showed potential antioxidant activity based on the above four antioxidant methods. In short, IL-UAE was first employed to develop a novel and green extraction method for TBF content, and this experiment provides valuable references for further utilization of S. helvetica.

Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

2011 ◽  
pp. 151-157 ◽  
Author(s):  
Marijana B. Saka ◽  
Julianna F. Gyura ◽  
Aleksandra Mišan ◽  
Zita I. Šereš ◽  
Biljana S. Pajin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Shelf Life ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
Room Temperature ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

scholarly journals Optimization of banana crop by-products solvent extraction for the production of bioactive compounds

2021 ◽  
Author(s):  
Sara Díaz ◽  
Antonio N. Benítez ◽  
Sara Ramírez-Bolaños ◽  
Lidia Robaina ◽  
Zaida Ortega
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Ethanol ◽  
Ethanol Concentration ◽  
Extraction Process ◽  
Total Phenol Content ◽  
Optimal Conditions ◽  
Satisfactory Description ◽  
Optimized Conditions ◽  
By Products ◽  
Banana Crops

AbstractThe aim of this work is the optimization of phenolic compound extraction from three by-products of banana crops (rachis, discarded banana, and banana’s pseudostem pulp), as a way to valorize them through a green extraction process. The influence of the temperature and aqueous ethanol concentration (Et-OH) on extract properties (total phenol content (TPC) and antioxidant activity) was firstly analyzed. 78 ℃ and ethanol concentrations close to 50% yielded the best results for the three materials. The equations obtained by the response surface methodology gave a satisfactory description of the experimental data, allowing optimizing the extraction conditions. Under optimized conditions, time influence was then assessed, although this parameter seemed not influence results. Among the three by-products, rachis extract (60% Et-OH, 78 ℃, and 30 min) presented the highest TPC (796 mg gallic acid/100 g of dried material) and antioxidant activity (6.51 mg Trolox equivalents/g of dried material), followed by discarded banana, and pseudostem pulp. Under the optimal conditions, experiments were performed at a larger scale, allowing to determine the extraction yields (EY) and to characterize the extracts. The highest EY was obtained for the rachis (26%), but the extract with the highest activity was obtained for discarded banana (50% Et-OH, 78 ℃, and 60 min), which presented a TPC of 27.26 mg/g extract corresponding to 54.59 mg Trolox equivalents/g extract. This study contributes to the valorization of banana crops residues as a source of polyphenolic compounds with bioactive functions that can be extracted under economic extraction conditions. Graphical abstract

scholarly journals Longevity of Raw and Lyophilized Crude Urease Extracts

2021 ◽  
Vol 2 (2) ◽  
pp. 325-334
Author(s):  
Neda Javadi ◽  
Hamed Khodadadi Tirkolaei ◽  
Nasser Hamdan ◽  
Edward Kavazanjian
Keyword(s):  
Crude Extract ◽  
Room Temperature ◽  
Low Cost ◽  
Extraction Process ◽  
Crude Extracts ◽  
Simple Extraction ◽  
Commercial Applications ◽  
Stable Source ◽  
The Stability ◽  
The Cost

The stability (longevity of activity) of three crude urease extracts was evaluated in a laboratory study as part of an effort to reduce the cost of urease for applications that do not require high purity enzyme. A low-cost, stable source of urease will greatly facilitate engineering applications of urease such as biocementation of soil. Inexpensive crude extracts of urease have been shown to be effective at hydrolyzing urea for carbonate precipitation. However, some studies have suggested that the activity of a crude extract may decrease with time, limiting the potential for its mass production for commercial applications. The stability of crude urease extracts shown to be effective for biocementation was studied. The crude extracts were obtained from jack beans via a simple extraction process, stored at room temperature and at 4 ℃, and periodically tested to evaluate their stability. To facilitate storage and transportation of the extracted enzyme, the longevity of the enzyme following freeze drying (lyophilization) to reduce the crude extract to a powder and subsequent re-hydration into an aqueous solution was evaluated. In an attempt to improve the shelf life of the lyophilized extract, dextran and sucrose were added during lyophilization. The stability of purified commercial urease following rehydration was also investigated. Results of the laboratory tests showed that the lyophilized crude extract maintained its activity during storage more effectively than either the crude extract solution or the rehydrated commercial urease. While incorporating 2% dextran (w/v) prior to lyophilization of the crude extract increased the overall enzymatic activity, it did not enhance the stability of the urease during storage.

scholarly journals Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2494
Author(s):  
Wei Wang ◽  
Yu-Ting Gao ◽  
Ji-Wen Wei ◽  
Yin-Feng Chen ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Ph Value ◽  
Soxhlet Extraction ◽  
Passion Fruit ◽  
Total Phenol Content ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Dpph Scavenging

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

Optimization of the Extraction Technology and Assessment of Antioxidant Activity of Chlorogenic Acid-Rich Extracts From Eucommia ulmoides Leaves

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110461
Author(s):  
Hua Jiang ◽  
Jun Li ◽  
Ning Zhang ◽  
Hai-Yang He ◽  
Jia-Min An ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Ph Value ◽  
Extraction Process ◽  
Eucommia Ulmoides ◽  
Extraction Technology ◽  
Dpph Method ◽  
Contour Plots ◽  
Technology And Assessment ◽  
Design Optimum

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic acid has been extended to the fields of food, medicine, health care and daily-use chemical industry. The aim of this research was to optimize the extraction conditions for chlorogenic acid from Eucommia ulmoides (Eucommiaceae) leaves. The significant variables were screened and optimized by a combination of Plackett-Burman test and Box-Behnken design. Optimum extraction parameters with ethanol concentration of 50%, solvent pH value of 3, and particle size of 60 mesh were determined according to variance analysis and contour plots. Under these conditions, the yield of chlorogenic acid was up to 4.36 mg/g, which was basically consistent with the theoretical prediction value of 4.50 mg/g. This study also proved the potential antioxidant activity of E. ulmoides leaves. The optimal extract of E. ulmoides leaves rich in chlorogenic acid showed the highest antioxidant activity in the FRAP method, which was 219.8 μM Trolox equivalents (TE) per g extract weight (EW) (μM TE/g EW). The DPPH method gave a similar value (168 μM TE/g EW) to the ABTS method (152 μM TE/g EW). The established extraction process was efficient in the recovery of chlorogenic acid from E. ulmoides leaves, encouraging its valorization as a cheap and sustainable alternative for the isolation of chlorogenic acid.

Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarp

2018 ◽  
Vol 7 (1) ◽  
pp. 51-55
Author(s):  
Suman Joshi DSD ◽  
◽  
Chander Amgoth ◽  
Surya Narayana S ◽  
Padmavathi CH ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Chronic Myeloid Leukaemia ◽  
Mtt Assay ◽  
Preparative Hplc ◽  
Spectroscopic Techniques ◽  
Anticancer Activities ◽  
Frap Assay ◽  
Aporphine Alkaloid ◽  
Dose Dependent

Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to isolate and purify the alkaloid. Based on NMR, FTIR and MS/MS spectroscopic techniques the compound was identified as Crebanine. This is the first report on the presence of Crebanine from the leaves of Alphonsea sclerocarpa. Antioxidant activity was evaluated using DPPH and FRAP assay. The antioxidant activity was dose-dependent and activity increased with the increase in the concentration. The cytotoxicity assay was carried out using MTT assay. The results showed remarkable cytotoxic activity against K562 (CML-chronic myeloid Leukaemia blood cancer) cells with a % inhibition of 37.545 at 500µm concentration after an incubation of 24h

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