scholarly journals Effect of prenatal Mozart composition on Brain Derived Neurotrophic Factor expression in cerebrum and cerebellum of Rattus norvegicus offspring from Food Restriction 50 model

2018 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Yulia Putri Permatasari ◽  
Widjiati Widjiati ◽  
Hermanto Tri Joewono

Objectives: To analyze the difference of BDNF expression on Rattus norvegicus offspring FR50 model in cerebrum and cerebellum between Mozart composition stimulation group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animal subjects Rattus norvegicus FR50 model. This study was conducted at animal laboratory, Faculty of Veterinary Medicine, Airlangga University. Animal subjects were divided into Mozart music stimulation group and control. The BDNF expression was analyzed using comparison test, with significancy p<0,05.Results: There was no difference of BDNF expression on Rattus norvegicus offspring FR50 model between Mozart music stimu-lation group and control in cerebrum with p=0.495 (mean Mozart group 6.89+2.52 vs control 6.22+1.99), and cerebellum with p=0.146 (mean Mozart group 7.41+2.67 vs control 5.73+2.45).Conclusions: There was no difference of BDNF expression between Mozart composition stimulation group and control in the cerebrum and cerebellum of Rattus norvegicus offspring FR50 model.

2018 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Yulia Putri Permatasari ◽  
Widjiati Widjiati ◽  
Hermanto Tri Joewono

Objectives: To analyze the difference of BDNF expression on Rattus norvegicus offspring FR50 model in cerebrum and cerebellum between Mozart composition stimulation group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animal subjects Rattus norvegicus FR50 model. This study was conducted at animal laboratory, Faculty of Veterinary Medicine, Airlangga University. Animal subjects were divided into Mozart music stimulation group and control. The BDNF expression was analyzed using comparison test, with significancy p<0,05.Results: There was no difference of BDNF expression on Rattus norvegicus offspring FR50 model between Mozart music stimu-lation group and control in cerebrum with p=0.495 (mean Mozart group 6.89+2.52 vs control 6.22+1.99), and cerebellum with p=0.146 (mean Mozart group 7.41+2.67 vs control 5.73+2.45).Conclusions: There was no difference of BDNF expression between Mozart composition stimulation group and control in the cerebrum and cerebellum of Rattus norvegicus offspring FR50 model.


2018 ◽  
Vol 26 (2) ◽  
pp. 48
Author(s):  
Andita Hapsari ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati

Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.


Author(s):  
Syania M O ◽  
Hermanto T J ◽  
Mudjiani B

Aim:To analyze the difference of the number of neurons in the cerebrum of Rattus norvegicus offsprings exposed to Mozart, Jazz, Blues, and Rock compilations during pregnancy. Method:Experimental study with single-blind randomized post-test only control group design using Rattus norvegicusas animal subjects that were divided into four groups: Mozart, Jazz, Blues, and Rock groups. The exposures started from the 10 day of gestation for 1 hour in a dark atmosphere with an intensity of 60 dB, in a distance of 25 cm from the exposure box, during March-May 2019. After cesarean delivery at day 19, the offsprings brain were prepared and stained by Hematoxylin-Eosin, then analyzed in 5 fields in each hemisphere with 1000x magnifying microscope. The study was conducted after ethical clearance and used a comparison statistical test chosen accordingly. Result:There were significant differences of the number of neurons between Mozart (71,96 ± 14,44) with Jazz (41,06 ± 7,65), Blues(40,92 ± 6,36), and Rock group (31,31 ± 8,19) with p=0,000. Conclusion:The number of neurons in the cerebrum of Rattus norvegicus offsprings exposed to Mozart compilation during pregnancy proved to be higher than Jazz,Blues, and Rock compilations.


Author(s):  
Budijanto Budijanto

Doing exercises regularly is useful to help improve strength, frmness, and endurance and also to avoid early aging process The best exercises to make body healthy are swimming, quick hiking, jogging, and cycling. The goal of the research was to verify the difference of influence toward aerobic and anaerobic exercises towards the change of cholesterol, cholesterol HDL and LDL as well as ratio of cholesterol HDL and LDL. The research was experimental using post test and control group. The sample was classifed into three groups, each of which consisting 10 mice and then randomly aerobic and anaerobic exercises and control group were done. The aerobic exercise by swimming used 3% burden of body weight for 13 minutes and the anaerobic exercise 9% for 0ne minute by taking rest for 3 minutes intermittently as many as 4 sets, while control group did it without exercise. Based on the data analysis, it was found that aerobic exercises was effective to reduce cholesterol, improve cholesterol of HDL and LDL, and ratio of cholesterol of HDL and LDL. In addition the aerobic exercise was better than anaerobic exercise


2019 ◽  
Vol 26 (3) ◽  
pp. 112
Author(s):  
Anwar Fauzi ◽  
Widjiati Widjiati ◽  
Hermanto T Joewono

Objectives: To analyze the influence of 50 percent food  restriction during pregnancy to the dendritic density of cerebellum and cerebellum of newborn Rattus norvegicus.Materials and Methods: Laboratory experimental study with single blind randomized post-test only control group design using animal model; pregnant Rattus norvegicus as treatment models. Subjects were divided into two groups: control group and treatment group which was exposed to 50% food restriction (FR 50%). At day 21, both group sacrificed and the cerebrum and cerebelum of the offsprings were prepared and stained with silver impregnation. We used parametric independent t-test in analyzing dendritic density.Results: In the cerebrum there was a significant difference in dendritic density between control (4.98+2.17) and treatment (2.69+0.76) groups with p=0.001 (p<0.05). In the cerebellum there was ALSO a significant difference in dendritic density between control (7.37+2.23) and treatment groups (3.01+0.64) with p=0.000 (p<0.05).Conclusions: The dendritic density of cerebrum and cerebellum of newborn Rattus norvegicusexposed to 50 percent of food restriction during pregnancy  were lower than control. 


2011 ◽  
Vol 35 (2) ◽  
pp. 117
Author(s):  
Netti Suharti ◽  
Andani Eka Putra

AbstrakVaksinasi BCG merupakan metoda pencegahan infeksi M. tuberculosis dengan rentang efektivitas antara 0 – 80%. Vaksin ini bekerja dengan meng-induksi sistem imunitas seluler, terutama sel limfosit T. Beberapa faktor yang mempengaruhi efektivitas vaksin, seperti bahan dasar, rute pemberian, penyimpanan dan lain sebagainya.penelitian ini bertujuan untuk menilai pengaruh rute vaksinasi BCG, per oral dan subkutan terhadap imunitas seluler dan humoral, yang meliputi pembentukan antibodi, kosentrasi sel limfosit B, T dan penilaian kemampuan makrofag.Penelitian eksperimental yang dilaksanakan dengan mengunakan desain post test only control group design. Jumlah sampel 10 ekor Rattus norvegicus galur Wistar. Antibodi diperiksa dengan menggunakan ELISA, serta dilakukan uji statistik mengunakan t-test.Didapatkan kadar antibodi kelompok subkutan pada hari ke-10 adalah OD 1.714 ± 0.124, sedangkan kelompok oral adalah 1.256 ± 0.243 dan pada kontrol kadar antibodi adalah 0.975 ± 0.253 (p < 0.05). Jumlah limfosit B pada kelompok subkutan adalah 4.5 x 103 sel/ml, kelompok oral 2.6 x 103 sel/ml dan kontrol 2.2. x 103 sel/ml (p < 0.05). Jumlah limfosit T kelompok subkutan adalah 3.8 x 104 sel/ml sedangkan pada kelompok oral dan kontrol, masing-masing adalah 1.1 x 104 sel/ml dan 0.8 x 104 sel/ml (p < 0.05). Kemampuan fagositosis makrofag subkutan adalah 62.5 ± 6.8 lebih tinggi dibanding kelompok oral 48.7 ± 5.8 dan kontrol 35.6 ± 4.4 (p < 0.05). Fenomena yang sama ditemukan pada hari ke-20. Namun demikian tidak ditemukan perbedaan konsentrasi seluruh variabel seluler dan humoral antara hari ke-10 dan 20 (p > 0.05).Vaksinasi BCG akan meningkatkan respon imunitas seluler dan humoral dan rute vaksinasi subkutan lebih baik dibanding per oral ditinjau dari aspek respon imun.Kata kunci : Vaksinasi BCG, seluler, humoral, makrofagAbstractBCG vaccine is a method to prevent the infection of M tuberculosis with effectiveness range between 0 – 80%. This vaccine works with inducing cellular immunity system, mainly in cell of Lymphocyte T. There are many factors thatARTIKEL PENELITIAN118influence the vaccines affectivity, such as basic substance, giving route,and storage.This experiment is conducted in to Rattus norvegicus Wistar Furrow in order to examine the effect of the route of BCG vaccination’s in oral and sub cutaneous to cellular and humoral immunity, which covers the formation of antibody, lymphocyte B cell concentration, and the evaluation of macrophage capability.This experiment’s result shows that the antibody of sub cutaneous groups on day 10th is OD 1.714 ± 0.124, and 1.256 ± 0.243 in oral, while in antibody control is 0.975 ± 0.253 (p < 0.05 ). The amount of Lymphocyte B in sub cutaneous group is 4.5 x 103 cell/ml, 2.6 x 103 cell/ml in oral and 2.2 x 103 cell/ml (p < 0.05) in control. The amount of Lymphocyte T in sub cutaneous is 3.8 x 104 cell/ml, while in oral and control is 1.1 x 104 cell/ml and 0.8 x 104 cell/ml (p < 0.05). The phagocytosis ability of sub cutaneous’s macrophage is 62.5 ± 6.8 which is higher than oral group 48.7 ± 5.8 and control 35.6 ± 4.4 (p < 0.05). The same phenomenon also found in day 20th. However, the difference concentrations of the entire cellular and humeral variable between day 10th and day 20th ( p < 0.05 ) has not been found.Based on the result of this experiment’s it can be concluded that BCG vaccination will increase the cellular and humeral immunity response and sub cutaneous’s vaccination route is better that oral, from immunes response aspect side.Key word : BCG Vaccination, cellular, humeral, macrophage.


2017 ◽  
Vol 5 (13) ◽  
pp. 52 ◽  
Author(s):  
Ahmet Uzun ◽  
Onder Karakoc

This study was conducted to determine the effects of ten weekly plyometric training on anaerobic power in judokas. 30 male judokas participated in the study and the subjects were divided into two groups as an experimental (15 male age = 21,40 ± 1,99) and control (15 male age = 21,53 ± 1,80) groups. Judo training programme was applied in both groups for 3 days / 90 minutes per week. Experimental and control group were made warm up exercises for 20 minutes. Both groups continued with the special preparatory period judo training program. After warm up exercises, experimental group judokas were made plyometric training consisting of 15 different movements for 20 minutes. Then they were allowed to continue the judo training. As the groups showed normal distribution, Paired Samples T-Test was applied for the significance between pre-test and post-test measurements of the groups. Independent Samples T-Test was used to analyze the difference between the test group and control group. The Independent Samples T-Test was used to analyze the difference between the experimental group and the control group. As a result of the plyometric training, when the pre-test and post-test differences of the physical measurement parameters for the experimental and control groups were compared, the mean values of back strength, anaerobic power and body fat percentage were found to be significant(p0.05). As a result, it is seen that the regular plyometric exercises increase the performance of anaerobic power to judokas. It can be said that putting plyometric training besides judo training has a positive effect for performance.


Author(s):  
Dang Thi Thu Huong ◽  

The current study determines the effectiveness of using task-based language instructions in teaching writing at a high school in Thai Nguyen province. The participants consist of 92 grade 10 students from Bing Yen high school, Dinh Hoa district. The study employs a mix method research design (MMR) as the plan for the research. The results of the study reveals that the results of writing performance were reported at a low level (M=3.72). The results also confirmed that the intervention worked well for the experimental group. The mean score of the pre-test (the baseline for both groups) was M=3.72. The intervention focused on TBLT instructions. After first five weeks, the means of post-test1 were M=4.96 and M=3.83 for experimental group and control group respectively. There was a significant difference between the mean of pre-test and post-test1 for the experimental group (M=3.72 compared to M=4.96), while the control group showed a little improvement (M=3.72 compared to M=3.83) the difference was not significant. After another five weeks, the difference in the mean scores of the experimental group were far more than the mean scores of the control group, M=5.56 and M=4.45 respectively. By the end of the fifteenth week of the intervention, the mean of post-test 3 of the experimental group reached M=6.41, the mean for control group was M=5.54. The difference of the mean scores in the pre-test and post-test revealed a significant improvement in both groups. However, the experimental group showed a greater improvement, M=6.41 and M=5.54 respectively. It can infer that the uses of TBLT writing instruction improved writing achievement greatly.


2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.


2018 ◽  
Vol 5 (1) ◽  
pp. 28
Author(s):  
Wiwik Alwiah ◽  
Mansur Akil

This study aimed at investigating the effectiveness of the use of the systemic approach to improve students’ understanding on adjectives materials related to the use, usage, synonym, antonym, spelling, pronunciation, collocation, meaning, and order. This research employed a quasi-experimental design. The populations were the third-semester students of Muhammadiyah University of Makassar in academic year 2017/2018. The sample consisted of 40 students chosen from two classes by using purposive sampling technique then were divided into two groups, namely experimental and control group. The difference between experimental and control class was based on the teaching technique used during the treatment. While the experimental class was taught by using systemic approach, control class was taught by using the conventional method.The instruments of this research were adjective tests. The data was analyzed by using statistical analysis on SPSS for Windows 20.0 program. As per data, experimental group experienced improvement on their understanding on adjectives in terms of use, usage, meaning, spelling, pronunciation, synonym, antonym, collocation, and order indicated by the post-test mean score (77.1) which was significantly higher than that of the control class (59.7) and the gain of experimental group (31.2) which was higher than control group (17.4). Another supporting indicator is the t-test value which was higher than the t-table value (4.022 > 1.75). Furthermore, the researcher found that the element of adjective which experienced most significant increase on its mastery happened on the usage, while the lowest one was the collocation. The result of this research indicated that there was significant improvement between experimental and control class. It means that systemic approach that was applied in experimental group can improve the students’ mastery on adjectives material. Thus it was concluded that the systemic approach was effective to improve students’ on adjectives.


Sign in / Sign up

Export Citation Format

Share Document