scholarly journals PENGARUH PEMBERIAN VAKSIN BCG SECARA ORAL DAN SUBKUTAN TERHADAP KOMPONEN SELULER DAN HUMORAL PADA Rattus norvegicus GALUR WISTAR

2011 ◽  
Vol 35 (2) ◽  
pp. 117
Author(s):  
Netti Suharti ◽  
Andani Eka Putra
Keyword(s):  
Rattus Norvegicus ◽  
Control Group ◽  
Control Group Design ◽  
Bcg Vaccination ◽  
Post Test ◽  
Oral Group ◽  
The Difference ◽  
Cellular And Humoral Immunity ◽  
Per Oral ◽  
And Control

AbstrakVaksinasi BCG merupakan metoda pencegahan infeksi M. tuberculosis dengan rentang efektivitas antara 0 – 80%. Vaksin ini bekerja dengan meng-induksi sistem imunitas seluler, terutama sel limfosit T. Beberapa faktor yang mempengaruhi efektivitas vaksin, seperti bahan dasar, rute pemberian, penyimpanan dan lain sebagainya.penelitian ini bertujuan untuk menilai pengaruh rute vaksinasi BCG, per oral dan subkutan terhadap imunitas seluler dan humoral, yang meliputi pembentukan antibodi, kosentrasi sel limfosit B, T dan penilaian kemampuan makrofag.Penelitian eksperimental yang dilaksanakan dengan mengunakan desain post test only control group design. Jumlah sampel 10 ekor Rattus norvegicus galur Wistar. Antibodi diperiksa dengan menggunakan ELISA, serta dilakukan uji statistik mengunakan t-test.Didapatkan kadar antibodi kelompok subkutan pada hari ke-10 adalah OD 1.714 ± 0.124, sedangkan kelompok oral adalah 1.256 ± 0.243 dan pada kontrol kadar antibodi adalah 0.975 ± 0.253 (p < 0.05). Jumlah limfosit B pada kelompok subkutan adalah 4.5 x 103 sel/ml, kelompok oral 2.6 x 103 sel/ml dan kontrol 2.2. x 103 sel/ml (p < 0.05). Jumlah limfosit T kelompok subkutan adalah 3.8 x 104 sel/ml sedangkan pada kelompok oral dan kontrol, masing-masing adalah 1.1 x 104 sel/ml dan 0.8 x 104 sel/ml (p < 0.05). Kemampuan fagositosis makrofag subkutan adalah 62.5 ± 6.8 lebih tinggi dibanding kelompok oral 48.7 ± 5.8 dan kontrol 35.6 ± 4.4 (p < 0.05). Fenomena yang sama ditemukan pada hari ke-20. Namun demikian tidak ditemukan perbedaan konsentrasi seluruh variabel seluler dan humoral antara hari ke-10 dan 20 (p > 0.05).Vaksinasi BCG akan meningkatkan respon imunitas seluler dan humoral dan rute vaksinasi subkutan lebih baik dibanding per oral ditinjau dari aspek respon imun.Kata kunci : Vaksinasi BCG, seluler, humoral, makrofagAbstractBCG vaccine is a method to prevent the infection of M tuberculosis with effectiveness range between 0 – 80%. This vaccine works with inducing cellular immunity system, mainly in cell of Lymphocyte T. There are many factors thatARTIKEL PENELITIAN118influence the vaccines affectivity, such as basic substance, giving route,and storage.This experiment is conducted in to Rattus norvegicus Wistar Furrow in order to examine the effect of the route of BCG vaccination’s in oral and sub cutaneous to cellular and humoral immunity, which covers the formation of antibody, lymphocyte B cell concentration, and the evaluation of macrophage capability.This experiment’s result shows that the antibody of sub cutaneous groups on day 10th is OD 1.714 ± 0.124, and 1.256 ± 0.243 in oral, while in antibody control is 0.975 ± 0.253 (p < 0.05 ). The amount of Lymphocyte B in sub cutaneous group is 4.5 x 103 cell/ml, 2.6 x 103 cell/ml in oral and 2.2 x 103 cell/ml (p < 0.05) in control. The amount of Lymphocyte T in sub cutaneous is 3.8 x 104 cell/ml, while in oral and control is 1.1 x 104 cell/ml and 0.8 x 104 cell/ml (p < 0.05). The phagocytosis ability of sub cutaneous’s macrophage is 62.5 ± 6.8 which is higher than oral group 48.7 ± 5.8 and control 35.6 ± 4.4 (p < 0.05). The same phenomenon also found in day 20th. However, the difference concentrations of the entire cellular and humeral variable between day 10th and day 20th ( p < 0.05 ) has not been found.Based on the result of this experiment’s it can be concluded that BCG vaccination will increase the cellular and humeral immunity response and sub cutaneous’s vaccination route is better that oral, from immunes response aspect side.Key word : BCG Vaccination, cellular, humeral, macrophage.

scholarly journals Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto
Keyword(s):  
Rattus Norvegicus ◽  
Exercise Program ◽  
The Body ◽  
Control Group ◽  
Regular Exercise ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

scholarly journals Mozart Compilation During Pregnancy Gave Higher Number of Neurons of Rattus Norvegicus Offsprings in Cerebrum Compared to Jazz, Blues, and Rock Compilation

2019 ◽  
pp. 1-8
Author(s):  
Syania M O ◽  
Hermanto T J ◽  
Mudjiani B
Keyword(s):  
Rattus Norvegicus ◽  
Statistical Test ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
The Difference ◽  
Single Blind ◽  
Hematoxylin Eosin ◽  
Ethical Clearance

Aim:To analyze the difference of the number of neurons in the cerebrum of Rattus norvegicus offsprings exposed to Mozart, Jazz, Blues, and Rock compilations during pregnancy. Method:Experimental study with single-blind randomized post-test only control group design using Rattus norvegicusas animal subjects that were divided into four groups: Mozart, Jazz, Blues, and Rock groups. The exposures started from the 10 day of gestation for 1 hour in a dark atmosphere with an intensity of 60 dB, in a distance of 25 cm from the exposure box, during March-May 2019. After cesarean delivery at day 19, the offsprings brain were prepared and stained by Hematoxylin-Eosin, then analyzed in 5 fields in each hemisphere with 1000x magnifying microscope. The study was conducted after ethical clearance and used a comparison statistical test chosen accordingly. Result:There were significant differences of the number of neurons between Mozart (71,96 ± 14,44) with Jazz (41,06 ± 7,65), Blues(40,92 ± 6,36), and Rock group (31,31 ± 8,19) with p=0,000. Conclusion:The number of neurons in the cerebrum of Rattus norvegicus offsprings exposed to Mozart compilation during pregnancy proved to be higher than Jazz,Blues, and Rock compilations.

scholarly journals Pengaruh Latihan Aerobik Dan Anaerobik Terhadap Profl Lipid Pada Tikus Rattus Norvegicus Wistar

2018 ◽  
Vol 24 (2) ◽  
pp. 41-48
Author(s):  
Budijanto Budijanto
Keyword(s):  
Aerobic Exercise ◽  
Rattus Norvegicus ◽  
Aging Process ◽  
Anaerobic Exercise ◽  
Control Group ◽  
Post Test ◽  
The Difference ◽  
And Control ◽  
Aerobic And Anaerobic ◽  
Better Than

Doing exercises regularly is useful to help improve strength, frmness, and endurance and also to avoid early aging process The best exercises to make body healthy are swimming, quick hiking, jogging, and cycling. The goal of the research was to verify the difference of influence toward aerobic and anaerobic exercises towards the change of cholesterol, cholesterol HDL and LDL as well as ratio of cholesterol HDL and LDL. The research was experimental using post test and control group. The sample was classifed into three groups, each of which consisting 10 mice and then randomly aerobic and anaerobic exercises and control group were done. The aerobic exercise by swimming used 3% burden of body weight for 13 minutes and the anaerobic exercise 9% for 0ne minute by taking rest for 3 minutes intermittently as many as 4 sets, while control group did it without exercise. Based on the data analysis, it was found that aerobic exercises was effective to reduce cholesterol, improve cholesterol of HDL and LDL, and ratio of cholesterol of HDL and LDL. In addition the aerobic exercise was better than anaerobic exercise

scholarly journals Default sequence of Mozart’s compositions during pregnancy gave higher dendritic density in the cerebrum and cerebellum of Rattus norvegicus offsprings compared with reversed sequence and control

2019 ◽  
Vol 27 (1) ◽  
pp. 5
Author(s):  
Harry Mangasi Binsar Panjaitan ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Experimental Study ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Exposure Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Objectives: This study aim to determine sequence of Mozart songs in the analyzing differences in dendritic density of cerebellum and cerebellum of new born Rattus novergicusMaterials and Methods: Experimental study randomized post test only control group design using Rattus norvegicus. Animal subjects were divided into three groups which were control group, and the treatment group that were given exposure to default sequence and reversed sequence of Mozart's music from gestation day 10. We used a comparison test in the analysis expression of BDNF.Results: In the cerebrum there was a significant difference in the Mozart group in reverse rather than with Mozart standard sequence and in standard Mozart group with no exposure group, with p=0.003 and p=0.000. In the cerebellum there was a significant difference in the group in reverse rather than with standard Mozart and in the standard Mozart group with the control group, with p=0.000 and p=0.000. However, there was no significant difference between control group and Mozart group upside down in cerebrum and cerebellum with p=0.109 and p=0.077Conclusion: The density of dendrites in the cerebrum and cerebellum of Rattus norvegicus newborn exposed to Mozart's music during pregnancy with standard Mozart sequence was higher than that in those receiving reverse order and without exposure. There were no significant differences between the density of the cerebrum and cerebellum dendrites between groups exposed to reversed sequence of Mozart composition and those without exposure.

scholarly journals PENGARUH EKSTRAK MASERASI TEH HIJAU TERHADAP APOPTOSIS SEL GRANULOSA FOLIKEL OVARIUM PADA RATTUS NORVEGICUS YANG DIPAJAN MSG

2019 ◽  
Vol 4 (1) ◽  
pp. 348
Author(s):  
Endah Wijayanti ◽  
Tatit Nurseta ◽  
Eviana Norahmawati
Keyword(s):  
Green Tea ◽  
Rattus Norvegicus ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Tea Extract ◽  
Per Oral

Abstract Monosodium glutamate is one of the additives used a food flavoring and is widely known by society. The consumption of MSG in large quantities leads to the lesions of the hypothalamus nucleus, leading to some changes in the reproductive system. The aim of this study is to find out the influence of green tea extract against Apoptosis cells Granulose ovarian follicle Rattus norvegicus Dipajan MSG. The research method of using a true experimental research approach post test only control group Design. The sample used was a female white rat (Rattus norvegicus) the number of 25 tails divided into 5 groups. Exposure of monosodium glutamate administered at a dose of 0.7 mg/grBB per oral and green tea extract a variety of doses administered 2 hours thereafter during 30 days after the rats were obtained in the Proestrus phase. Apoptosis examination uses In Situ Cell Death Detection Kit, POD catalog number 11684817910 of Roche brand with Tunnel method. Data were analyzed using the One Way ANOVA test. Results showed that the lowest average apoptosis rate was seen in the MSG Administration treatment group and green tea extract dose 1.4 mg/tail/day (18,686 ± 2,247) but increased again on the group's MSG administration treatment and green tea extract dose 2.8 mg/tail/day (23,752 ± 3,206). So the administration of green tea extract of varying doses can decrease apoptosis in the Rattus norvegicus that is Dipajan MSG.   Keywords: green tea extract, monosodium glutamat, Apoptosis     Abstrak Monosodium glutamat, merupakan salah satu zat aditif yang digunakan sebagai penyedap makanan dan dikenal secara luas oleh masyarakat. Konsumsi MSG dalam jumlah besar menyebabkan lesi bagian nucleus arkuata hipotalamus sehingga menimbulkan beberapa perubahan pada sistem reproduksi. Tujuan dalam penelitian ini untuk mengetahui pengaruh ekstrak teh hijau terhadap Apoptosis Sel Granulosa Folikel Ovarium Rattus norvegicus yang dipajan MSG. Metode Penelitian menggunakan penelitian true eksperimental pendekatan post test only control group design. Sampel yang digunakan adalah tikus putih betina (Rattus norvegicus) sejumlah 25 ekor yang terbagi menjadi 5 kelompok. Paparan monosodium glutamat diberikan dengan dosis 0,7 mg/grBB per oral dan ekstrak teh hijau berbagai dosis diberikan 2 jam setelahnya selama 30 hari setelah didapatkan tikus berada pada fase proestrus. Pemeriksaan Apoptosis menggunakan In Situ Cell Death Detection Kit, POD nomor katalog 11684817910 merk Roche dengan metode Tunel. Data dianalisis menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa Nilai rerata apoptosis terendah tampak pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,4 mg/ekor/hari (18.686± 2.247) namun meningkat lagi pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 2,8 mg/ekor/hari (23.752± 3.206). Sehingga pemberian ekstrak teh hijau berbagai dosis dapat menurunkan apoptosis pada Rattus norvegicus yang dipajan MSG   Kata Kunci : ekstrak teh hijau, monosodium glutamat,  dan Apoptosis

scholarly journals Hepatoprotective Effect of Sun Chlorella as an Antioxidant in Rattus norvegicus Induced Carbon Tetrachloride

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Wahyu Siswandari ◽  
Fitranto Arjadi ◽  
Hernayanti Hernayanti ◽  
Vitasari Indriani
Keyword(s):  
Carbon Tetrachloride ◽  
Rattus Norvegicus ◽  
Hepatoprotective Effect ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Chlorella Sp ◽  
Randomized Design ◽  
Post Test ◽  
Per Oral

Carbon tetrachloride (CCl₄) is a chemical that can cause damage to liver cells. One of the natural ingredients developed to reduce liver damage due to chemicals and infections is Chlorella sp. The research aimed to explore the hepatoprotective effect of the antioxidant superoxide dismutase (SOD) by administering Sun Chlorella in Rattus norvegicus CCl₄ induced rats. The study design used a post-test with a control group design with a completely randomized design trial on 30 male rats of Wistar strains, aged 2–3 months, 200–250 grams in weight. The research was conducted in November 2016–January 2017 in Purwokerto. Rats were divided into five groups and treated for four weeks as follows: K1 group was given aquades; K2 was given CCl₄; K3, K4, K5 were given CCl₄ and Sun Chlorella 3.6 mg/200 gBW, 7.2 mg/200 gBW, and 14.4 mg/200 gBW respectively, by the gastric probe. Statistical analysis with correlation test and one way ANOVA multivariate test showed that Sun Chlorella 7.2 and 14.4 mg/200 gBW significantly increased SOD levels in rats induced CCl₄ (p=0.004, p=0.009). SOD rates were significantly strong associated with aspartate aminotransferase/AST (r=−0.685, p=0.000) and alanine aminotransferase/ALT (r=−0.659, p=0.000). The conclusion is Sun Chlorella increases SOD levels in CCl₄-induced rats. Increased SOD levels may decrease AST and ALT levels. EFEK HEPATOPROTEKTIF SUN CHLORELLA SEBAGAI ANTIOKSIDAN PADA RATTUS NORVEGICUS YANG DIINDUKSI KARBON TETRAKLORIDAKarbon tetraklorida (CCl₄) merupakan bahan kimia yang dapat menyebabkan kerusakan sel hati. Berbagai bahan alami telah dikembangkan untuk mengurangi kerusakan hati baik akibat bahan kimia maupun infeksi, salah satunya adalah Chlorella sp. Penelitian ini bertujuan melihat efek hepatoprotektif  dengan pemberian Sun Chlorella pada tikus Rattus norvegicus yang diinduksi CCl₄. Desain penelitian menggunakan post-test with a control group dengan rancangan percobaan rancangan acak lengkap pada 30 tikus jantan galur Wistar, usia 2–3 bulan, dan berat 200–250 gram. Penelitian ini dilakukan di Purwokerto pada periode November 2016–Januari 2017. Tikus dibagi menjadi lima kelompok dan mendapatkan perlakuan per oral dengan sonde lambung selama 4 minggu sebagai berikut: kelompok K1 sebagi kontrol negatif diberikan aquades; kelompok control positif K2 diberikan CCl₄; kelompok perlakuan K3, K4, dan K5 diberikan CCl4 dan Sun Chlorella 3,6 mg/200 gBB; 7,2 mg/200 gBB; 14,4 mg/200 gBB tikus berurutan. Uji statistik dengan menggunakan uji korelasi dan ANOVA satu arah menunjukkan bahwa pemberian Sun Chlorella 7,2 mg dan 14,4 mg meningkatkan kadar SOD pada tikus yang diinduksi CCl₄ secara bermakna (p=0,004; p=0,009). Kadar SOD berhubungan kuat dengan kadar aspartat aminotransferase/AST (r=−0,685; p=0,000) dan alanin aminotransferase/ALT (r=−0,659; p=0,000). Kesimpulan penelitian ini adalah pemberian Sun Chlorella meningkatkan kadar SOD pada tikus yang diinduksi CCl₄. Peningkatan kadar SOD menyebabkan penurunan kadar AST dan ALT.

scholarly journals PENGARUH EKSTRAK MASERASI TEH HIJAU TERHADAP APOPTOSIS SEL GRANULOSA FOLIKEL OVARIUM PADA RATTUS NORVEGICUS YANG DIPAJAN MSG

2019 ◽  
Vol 2 (5) ◽  
pp. 348
Author(s):  
Endah Wijayanti ◽  
Tatit Nurseta ◽  
Eviana Norahmawati
Keyword(s):  
Green Tea ◽  
Rattus Norvegicus ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Tea Extract ◽  
Per Oral

Abstract Monosodium glutamate is one of the additives used a food flavoring and is widely known by society. The consumption of MSG in large quantities leads to the lesions of the hypothalamus nucleus, leading to some changes in the reproductive system. The aim of this study is to find out the influence of green tea extract against Apoptosis cells Granulose ovarian follicle Rattus norvegicus Dipajan MSG. The research method of using a true experimental research approach post test only control group Design. The sample used was a female white rat (Rattus norvegicus) the number of 25 tails divided into 5 groups. Exposure of monosodium glutamate administered at a dose of 0.7 mg/grBB per oral and green tea extract a variety of doses administered 2 hours thereafter during 30 days after the rats were obtained in the Proestrus phase. Apoptosis examination uses In Situ Cell Death Detection Kit, POD catalog number 11684817910 of Roche brand with Tunnel method. Data were analyzed using the One Way ANOVA test. Results showed that the lowest average apoptosis rate was seen in the MSG Administration treatment group and green tea extract dose 1.4 mg/tail/day (18,686 ± 2,247) but increased again on the group's MSG administration treatment and green tea extract dose 2.8 mg/tail/day (23,752 ± 3,206). So the administration of green tea extract of varying doses can decrease apoptosis in the Rattus norvegicus that is Dipajan MSG.   Keywords: green tea extract, monosodium glutamat, Apoptosis     Abstrak Monosodium glutamat, merupakan salah satu zat aditif yang digunakan sebagai penyedap makanan dan dikenal secara luas oleh masyarakat. Konsumsi MSG dalam jumlah besar menyebabkan lesi bagian nucleus arkuata hipotalamus sehingga menimbulkan beberapa perubahan pada sistem reproduksi. Tujuan dalam penelitian ini untuk mengetahui pengaruh ekstrak teh hijau terhadap Apoptosis Sel Granulosa Folikel Ovarium Rattus norvegicus yang dipajan MSG. Metode Penelitian menggunakan penelitian true eksperimental pendekatan post test only control group design. Sampel yang digunakan adalah tikus putih betina (Rattus norvegicus) sejumlah 25 ekor yang terbagi menjadi 5 kelompok. Paparan monosodium glutamat diberikan dengan dosis 0,7 mg/grBB per oral dan ekstrak teh hijau berbagai dosis diberikan 2 jam setelahnya selama 30 hari setelah didapatkan tikus berada pada fase proestrus. Pemeriksaan Apoptosis menggunakan In Situ Cell Death Detection Kit, POD nomor katalog 11684817910 merk Roche dengan metode Tunel. Data dianalisis menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa Nilai rerata apoptosis terendah tampak pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 1,4 mg/ekor/hari (18.686± 2.247) namun meningkat lagi pada kelompok perlakuan pemberian MSG dan ekstrak teh hijau dosis 2,8 mg/ekor/hari (23.752± 3.206). Sehingga pemberian ekstrak teh hijau berbagai dosis dapat menurunkan apoptosis pada Rattus norvegicus yang dipajan MSG   Kata Kunci : ekstrak teh hijau, monosodium glutamat,  dan Apoptosis

scholarly journals Apoptosis index of cerebrum and cerebellum neuronal cells in Rattus norvegicus neonates born from mothers treated with 50% food restriction during gestation

2018 ◽  
Vol 26 (2) ◽  
pp. 48
Author(s):  
Andita Hapsari ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Rattus Norvegicus ◽  
Food Restriction ◽  
Apoptotic Index ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Post Test ◽  
The Difference ◽  
And Control ◽  
Veterinary Faculty

Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.

scholarly journals Pemberian oral ekstrak kulit buah lemon (Citrus limon) menghambat peningkatan ekspresi MMP-1 (matrix metaloproteinase-1) dan penurunan jumlah kolagen pada tikus putih galur wistar jantan (Rattus norvegicus) yang dipajan sinar UV-B

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Ahmad M. Anshori ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Ultraviolet B ◽  
Control Group ◽  
Control Group Design ◽  
Lemon Peel ◽  
Post Test ◽  
Male Wistar Rats ◽  
Per Oral ◽  
Peel Extract

Abstract: Ultraviolet B (UVB) is a source of free radicals that accelerate aging process of the skin such as activating enzymes that degrade collagen and inhibit collagen production by inducing the expression of MMP-1. Lemon peel contains vitamin C, vitamin A, tannins and phenols which possess antioxidant activity and prevent oxidative stress. This study was aimed to prove that oral administration of lemon peel extract could decrease MMP-1 levels and increase the number of collagen in the UVB-induced male Wistar rats (Rattus norvegicus). This was a true experimental study with the post test only control group design. Subjects were 30 male Wistar rats (Rattus norvegicus), aged 2-3 months, body weight 120 g, divided into 3 groups, as follows: the control group (P0) which were not exposed to UVB; the treatment group 1 (P1) given aquabidest and UVB ray exposure; and the treatment group 2 (P2) given lemon peel extract and UVB ray exposure. After 15 days of treatment, all rats were anesthetized and their skin tissues were prepared for histological examination of MMP1 and collagen. The results showed that the average expression of MMP1 in P0 group was 22.02±3.20%; in the P1 group was 29.04±6.36%; and in P2 group was 7.98±2.76% (P <0.01). In addition, the average amount of collagen in P0 group was 70.01±2.99%; in the P1 group was 57.68±4.84%; and in P2 group was 77.45±4.29% (P <0.01). Conclusion: Oral administration of lemon peel extract could decrease the expression of MMP-1 and increase the amount of collagen in the UVB-induced male Wistar rats (Rattus norvegicus).Keywords: lemon peels, collagen, MMP-1, UVB Abstrak: Ultraviolet B (UVB) merupakan sumber radikal bebas yang mempercepat proses penuaan kulit dengan mengaktivasi enzim yang mendegradasi kolagen dan menghambat produksi kkolagen melalui induksi ekspresi MMP-1. Kulit buah lemon mengandung vitamin C, vitamin A, tanin, dan fenol yang memiliki aktivitas antioksidan dan mencegah stres oksidatif. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak kulit buah lemon per oral dapat menurunkan kadar MMP-1 dan meningkatkan jumlah kolagen pada tikus putih jantan galur Wistar (Rattus norvegicus) yang dipajan sinar UVB. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subjek penelitian ialah 30 ekor tikus putih jantan, galur Wistar (Rattus norvegicus), umur 2-3 bulan, dengan berat badan 120 gr yang dibagi menjadi 3 kelompok, masing-masing berjumlah 10 ekor tikus. Kelompok kontrol (P0) tidak dipapar sinar UVB; kelompok perlakuan 1 (P1) diberikan aquabides oral dan paparan sinar UVB; dan kelompok perlakuan 2 (P2) diberikan ekstrak kulit buah lemon oral dan paparan sinar UVB. Setelah 15 hari perlakuan, seluruh tikus dianestesi kemudian diambil jaringan kulitnya untuk dibuat preparat histologik dan dihitung jumlah kolagen dermisnya sebagai data post test. Hasil analisis menunjukkan rerata jumlah ekspresi MMP1 pada kelompok P0 ialah 22,02±3,20%; kelompok P1 ialah 29,04±6,36%; dan kelompok P2 ialah 7,98±2,76% (P <0,01). Rerata jumlah kolagen pada kelompok P0 ialah 70,01±2,99%; kelompok P1 ialah 57,68±4,84%; dan kelompok P2 ialah 77,45±4,29% (P <0,01). Simpulan: Pemberian ekstrak kulit buah lemon per oral dapat menurunkan ekspresi MMP-1 dan meningkatkan jumlah kolagen pada tikus putih jantan galur Wistar (Rattus norvegicus) yang dipajan sinar UVB. Kata kunci: kulit buah lemon, kolagen, MMP-1, UVB

scholarly journals Pengaruh Konseling Qur’ani Untuk Menurunkan Tingkat Depresi Pada Mahasiswa

2019 ◽  
Vol 6 (2) ◽  
pp. 121
Author(s):  
Shanty Komalasari
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Quasi Experimental ◽  
Depression Level ◽  
The Difference ◽  
Wilcoxon Score ◽  
And Control ◽  
Experimental Group

This research aims to know the difference between the experimental group given intervention with Quranic counseling and a control group without intervention. This is quasi-experimental research that used untreated control group design with pretest and posttest. The subjects of this research were 20 students of UIN Antasari Banjarmasin that have passed the screening test. They were divided into an experimental group and control group, each of which consisted of 10 people.  After the data were analyzed using non-parametric Mann-Whitney test, it was found out that  = - 1553; sig = 0.121 (p>0,05), which means there was no significant difference between the level of depression on students in the experimental group after the intervention and the level of depression on students in control group. Afterward, according to the analysis result on Wilcoxon score in the post-test and interview by the experimental group, it was found out that Z score = - 0,609a and p score = 0,542; therefore, p > 0,05. It means Quranic counseling is relatively effective to decrease the level of depression on the students. Despite there was no significant difference between the experimental group and control group, the depression level on the experimental group, however, did not increase even though the Quranic counseling had been given over two weeks before.

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