scholarly journals The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

2019 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Special Treatment ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals Tempe Flour Increase The Amount of Erythrocytes of White Male Rat (Rattus norvegicus) Wistar Strain Induced by Cigarette Smoke

2019 ◽  
Vol 5 (1) ◽  
pp. 56
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2 ◽  
Hydroxide Radicals ◽  
Group 1

Cigarette smoke was one of the free radical sources for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe because it contains antioxidants substances such as carotene, Vitamin E, isoflavone, and factor II antioxidants. The aim of this study is knowing the influence of giving tempe flour toward the amount of erythrocytes of white male rat (Rattus norvegicus) Wistar strain induced by cigarette smoke. The experiment used a post-test only-control group design method. The samples were white male rats Wistar strain aged of 3 to 4 months as much 30 rats with weight around 180-220 gram. The samples divided into three groups, that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given any treatment, it only got a standard feed, treatment group 1 got treatment and it was induced by cigarette smoke. Furthermore, treatment group 2 got standard feed, it was induced by cigarette smoke and was given tempe. Analysis of data used one-way anova showed the significance p-value = 0,036 that was <α (0,05). The result showed giving tempe flour increase the amounts of erythrocytes of white male rat (Rattus norvegicus) Wistar strain which was induced by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

2017 ◽  
Vol 3 (6) ◽  
pp. 702 ◽  
Author(s):  
Dwi Gayatri Nurcahyawati ◽  
Hani Plumeriastuti ◽  
Lilik Maslachah
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Oral Therapy ◽  
White Male ◽  
Male Rats ◽  
Male Rat ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Per Oral ◽  
Tuber Extract

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.  Key words: Eleutherine palmifolia, alloxan, kidney, histhopathology

scholarly journals Pengaruh Pemberian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Berat, Diameter, Tebal Epitel Epididimis, Motilitas dan Viabilitas Spermatozoa Tikus Putih Jantan (Rattus norvegicus)

2019 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Stephanie Lexy Louis ◽  
Salni Salni ◽  
Sri Nita
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Spermatozoa Motility ◽  
Epithelium Thickness ◽  
Group A ◽  
Ocimum Americanum

<p>Infertility is one of the reproductive problems that may affect both male and female. In a male, the occurrence of reproductive problems may be due to abnormal spermatogenesis processes and anatomic disorder. Kemangi leaves are an aphrodisiac it contains some secondary metabolites like flavonoid, steroid, tannin, and saponin. The objective of the present research was to determine the effect of feeding fractions of kemangi leaves (Ocimum americanum L.) on the epididymis weight, diameter, and epithelial thickness and the spermatozoa motility and viability of white male rat (Rattus norvegicus). The research was an experimental one, with posttest only group design. The research design used a Completely Random Design. The research sample consisted of 24 furrow white male mice Sprague Dawley, 60-74 days old, divided to control group, a fraction of n-hexane, a fraction of ethyl acetate, and a fraction of water-ethanol. The dosage used for the treatment group was 100 mg/kgBB/day, and the control group was given CMC 1%. The result of data analysis by using a One-Way Anova test showed that there were increases in the weight and diameter of the epididymis in the treatment group as compared to that of the control group (p=0,000). Likewise, both epididymis epithelium thickness and spermatozoa viability in the treatment group increased as compared to that of the control group (p=0.001), while spermatozoa motility was p=0,002. It could be concluded that the fractions of kemangi leaves increased the weight, diameter, and epithelium thickness of epididymis and spermatozoa motility and viability in-furrow white male rat Sprague Dawley.<strong></strong></p>

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals UJI EFEK ANALGETIK EKSTRAK ETANOL RIMPANG JAHE PUTIH (Zingiber officinale Rosc.var. Amarum) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 927
Author(s):  
Beatriks Lahamendu ◽  
Widdhi Bodhi ◽  
Jainer P. Siampa
Keyword(s):  
Treatment Group ◽  
White Male ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Analgesic Effects ◽  
Wistar Strain ◽  
Ginger Rhizome ◽  
Ethanol Extracts

ABSTRACTWhite Ginger Rhizome (Zingiber officinale Rosc.var. Amarum) contains secondary metabolites such as flavonoids, there are also several compounds such as gingerol, shogaol, and zhigeron which provide anti-inflamatory, antioxidant, analgesic, anticarsinogenic and cardiotonic effects. This study aims to determine the analgesic effect of ethanol extracts of white ginger rhizome with different doses, namely the treatment group 1, dose 0.006 g, the Treatment  Group 2, dose 0.012 g and the Treatment Group 3, dose 0.024 g tested on white male wistar strain rats. This study uses a laboratory experimental research design using male white rats as experimental animals. The result of the study using LSD showed a difference between the group of negative control and the group of positive control, and also the treatment group with extracts with significance value (0.014, 0.008, 0.012 and 0.005 ) the value <0.05, which indicates that according to the hypothesis namely rejecting H0 and accepting H1, which means significantly different between the negative control group with  positive control group and the treatment group with extracts, but there is no difference between the positive control group and the treatment group with extract which means there is no difference because they both have analgesic effects. From the results of the study it can be concluded that the ethanol extracts of white ginger rhizome at a dose 0.006 g, 0.012 g, and 0.024 g has analgesic effects in male white wistar strain rats can be seen from the decreasing number of rat responses (licks and jumps).Keywords: Analgesic, anova, White Ginger Rhizome, White male wistar strain ABSTRAKRimpang Jahe putih (Zingiber officinale Rosc.var amarum ) mengandung metabolit sekunder seperti flavonoid juga terdapat beberapa senyawa seperti gingerol, shogaol, dan zingeron yang memberikan efek antiinflamasi, antioksidan, analgetik, antikarsinogenik dan kardiotonik. Penelitian ini bertujuan untuk mengetahui adanya efek analgetik dari ekstrak etanol rimpang jahe putih dengan dosis yang berbeda-beda yaitu Kelompok Perlakuan 1 dosis 0,006 g, kelompok Perlakuan 2 dosis 0,012 g dan Kelompok Perlakuan 3 dosis 0,024 g yang diuji pada tikus putih jantan galur wistar. Penelitian ini menggunakan rancangan penelitian eksperimental laboratorium dengan menggunakan tikus putih jantan sebagai hewan percobaan. Hasil penelitian menggunakan LSD menunjukkan adanya perbedaan antara kelompok kontrol negatif dengan kelompok kontrol positif, dan juga kelompok perlakuan dengan ekstrak dengan nilai signifikansi (0,014, 0,008, 0,012 dan 0,005) nilai tersebut <0,05 yang menandakan bahwa sesuai hipotesis yaitu menolak H0 dan menerima H1 yang berarti berbeda secara signifikan antara kelompok kontrol negatif dengan kontrol positif dan kelompok perlakuan dengan ekstrak,tapi tidak terdapat perbedaan antara kelompok kontrol positif dengan kelompok perlakuan dengan ekstrak yang berarti tidak memiliki perbedaan karena sama-sama memiliki efek analgetik. Dari hasil penelitian dapat disimpulkan bahwa ekstrak etanol rimpang jahe putih dengan dosis 0,006 g, 0,012 g dan 0,024 g memiliki efek analgetik pada tikus putih jantan galur wistar dapat dilihat dari menurunnya jumlah respon tikus (jilatan dan lompatan).Kata kunci : Analgetik, anova, rimpang Jahe putih, tikus Putih jantan galur wistar

scholarly journals The correlation between exposure to cigarette smoke and the degree of mucosal epithelium-based dysplasia in Rattus norvegicus tongues

2019 ◽  
Vol 52 (4) ◽  
pp. 187
Author(s):  
Dorisna Prijaryanti ◽  
Diah Savitri Ernawati ◽  
Desiana Radithia ◽  
Hening Tuti Hendarti ◽  
Rosnah Binti Zain
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Staining Technique ◽  
Epithelial Dysplasia ◽  
Control Group ◽  
Severe Dysplasia ◽  
Research Subjects ◽  
Polycyclic Aromatic ◽  
Carcinogenic Substances

Background: Cigarette smoke contains various carcinogenic substances such as polycyclic aromatic hydrocarbons and nitrosamines. These chemicals not only have the potential to damage DNA, but can also induce genetic mutations and activate genes that function during apoptosis. Thus, if the gene is dysregulated, it will cause cells to survive, proliferate and subsequently lead to the development of cancerous ones. Histologically, the carcinogenic process affecting the oral cavity starts with hyperplasia and dysplasia, followed by severe dysplasia then leading to invasive cancer and metastatic processes in other bodies. Purpose: This study aims to reveal the correlation between exposure to cigarette smoke and the degree of epithelial dysplasia evident in research subjects. Methods: This study used 27 samples of Rattus norvegicus tongue, divided into three groups, namely; a control group, a treatment group subjected to four weeks’ exposure to cigarette smoke, and a treatment group subjected to exposure lasting eight weeks. Each rat was placed in an individual chamber and exposed to smoke from 20 cigarettes introduced by a pump via a pipe for 7.5 minutes. The degree of epithelial dysplasia in each case was subsequently observed microscopically using HE staining technique. Results: Mild epithelial dysplasia increased by 0.82%, during the fourth week of exposure to cigarette smoke and by 2.99% during the eighth week. Similarly, moderate epithelial dysplasia rose by 5.29% during the fourth week of exposure and 5.99% during the eighth week. Severe epithelial dysplasia also increased by 2.2% during the fourth week of exposure and by 2.66% during the eighth week. Conclusion: The longer the exposure to cigarette smoke, the higher the degree of ensuing dysplasia.

scholarly journals The Effect of Propofol on Onset and Sedation in Diabetic Mellitus Rat (Rattus norvegicus)

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Erwin Erwin ◽  
Rusli Rusli ◽  
Zuraidawati Zuraidawati ◽  
Fadillah Irwansyah
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Diabetic Mellitus ◽  
Wistar Strain ◽  
Female Wistar Rats ◽  
Ad Libitum ◽  
Group 2 ◽  
Group 1

This research aimed to examine the time of onset and sedation on diabetic mellitus (DM) rat (Rattus norvegicus) by propofol. This study used 8 female wistar rats 2-3 months old and 150-200 grams of bodyweight, fed with standard feed and water ad libitum. Samples were divided into two groups. Group 1 (KI) as a control group was injected intraperitoneally with aloxan solvent and group 2 (KII) was injected intraperitoneally with 150 mg/kg bodyweight aloxan. Blood glucose was checked 10 days after aloxan injection, DM was categorized if the blood glucose over 150 mg/dl. All rats were injected intravenously with propofol 1% with the dose of 10 mg/kg bodyweight. Onset and sedation time was counted using stopwatch after propofol injection. The result showed that mean (±SD) of onset on KI and KII were 1.39±0.49 and 8.64±1.23 seconds, consecutively and they were significantly different at P0.01. Whilst mean (±SD) of sedation KI and KII were 12.12±1.47 minutes and 7.62±1.61 minutes, respectively. The onset and sedation time of KI was significantly different from KII P0.01. The conclusion of this research was DM affects time of onset and sedation when wistar strain rats were anesthetized by 1% propofol.Key words: rats, diabetic mellitus, propofol, onset, sedation

scholarly journals Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons

2018 ◽  
Vol 25 (3) ◽  
pp. 97
Author(s):  
Eka Nasrur Maulana ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati ◽  
Windhu Purnomo
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Apoptotic Index ◽  
Assay Method ◽  
Optimal Time ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control group's neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found  the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.

Pengaruh Pemberian Ekstrak Biji Petai (Parkia speciosa) Terhadap Gambaran Histopatologi Ginjal Bagian Tubulus Proksimal Pada Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Paracetamol

2018 ◽  
Vol 15 (2) ◽  
pp. 233
Author(s):  
AVINDA DEVIANA
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Positive Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
Standard Diet ◽  
Post Test ◽  
Inflammatory Drugs ◽  
Group 2 ◽  
Group 1

<p>Paracetamol, which is one of the NSAIDs (<em>Non Steroidal Anti Inflammatory Drugs</em>) is widely used by the wider community as a therapeutic and pain-reducing therapy. In addition to its many uses, paracetamol can cause a variety of serious toxicities, including nephrotoxicity. Bitter bean extract (<em>Parkia speciosa</em>) has a flavonoid compound which is an antioxidant. This was a laboratory experimental study with <em>post-test only control</em> <em>group </em>design using 24 rats divided into 3 groups. Positive control group fed with standard diet, aquades and bitter bean extract (<em>Parkia speciosa</em>); each rat in the treatment group 1 was induced with paracetamol 9 mg / day orally; and each rat in the treatment group 2 was given bitter bean extract (<em>Parkia speciosa</em>) 7,2 mg / day induced paracetamol 9 mg / day after 45 min. The kidneys were taken by surgery on the 15th day and microscopically examined. The data were analyzed using IBM SPSS version 23 with Kruskal-Wallis method.</p>

scholarly journals Perbedaan efek pemberian tunggal vitamin E dengan pemberian kombinasi vitamin e dan zink terhadap kualitas spermatozoa tikus wistar (Rattus Norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Iis A.H. Silalahi ◽  
Grace L.A. Turalaki ◽  
Janette M. Rumbajan
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Enzymatic Antioxidant ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Smoking can increase free radicals in sperm, which can damage sperm. Vitamin E as non-enzymatic antioxidant and zinc as enzymatic antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin E and zinc on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. The research used a completely randomized experimental design. Samples were 9 male wistar rats (Rattus norvegicus) which were randomly divided into 3 groups. Each group was given exposure to cigarette smoke from 2 bars of cigarette/day, which treatment group (P1) also was given vitamin E 1,44 mg/day and treatment group (P2) also was given combination of vitamin E 1,44 mg/day and zinc 10 mg/day. The treatment was given for 50 days. The results of this research showed a significant difference in the motility and morphology of spermatozoa between the treatment group (P1) which received exposure the cigarette smoke and vitamin E and treatment group (P2) which received exposure to cigarette smoke and combination of vitamin E and zinc (p<0,05) compared with the group which received exposure only cigarette smoke without vitamin E or zinc. The motility of spermatozoa treatment group (P2) which received exposure the cigarette smoke and combination vitamin E and zinc was found significant difference with the treatment group (P1) which received exposure the cigarette smoke and vitamin E (p <0,05). Conclusion: The conclusion from this research showed that there is difference between spermatozoa quality especially motility and morphology of group treated with vitamin E and combination of vitamin E and zinc after exposure to cigarette smoke, with combination of vitamin E and zinc had an higher average on spermatozoa quality especially motility.Keywords: vitamin E, zinc, cigarette smoke, quality of spermatozoa Abstrak: Merokok dapat menyebabkan peningkatan radikal bebas yang dapat merusak sperma. Vitamin E sebagai antioksidan non enzimatis dan zink sebagai antioksidan enzimatis merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin E dan kombinasi vitamin E dengan zink terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak legkap. Sampel yang digunakan adalah 9 tikus wistar (Rattus norvegicus) jantan yang dibagi secara acak menjadi 3 kelompok. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi vitamin E 1,44 mg/hari, dan kelompok perlakuan (P2) diberi kombinasi vitamin E 1,44 mg/hari dan zink 10 mg/hari. Perlakuan diberikan selama 50 hari. Hasil penelitian menunjukkan adanya perbedaan signifikan pada motilitas dan morfologi spermatozoa kelompok perlakuan (P1) dengan perlakuan paparan asap rokok dan vitamin E dan kelompok perlakuan (P2) dengan perlakuan paparan asap rokok dan kombinasi vitamin E dengan zink dibandingkan dengan kelompok control (P0) yang hanya mendapat paparan asap rokok (p<0,05). Motilitas spermatozoa kelompok perlakuan (P2) didapati hasil yang berbeda signifikan dibandingkan dengan kelompok perlakuan (P1) (p<0,05). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan signifikan kualitas spermatozoa yaitu pada motilitas dan morfologi antara pemberian tunggal vitamin E dan kombinasi vitamin E dengan zink setelah pemaparan asap rokok, dengan kombinasi vitamin E dan zink memiliki rata-rata kualitas spermatozoa lebih tinggi khususnya motilitas.Kata kunci: vitamin E, zink, asap rokok, kualitas spermatozoa

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