scholarly journals Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

2017 ◽  
Vol 3 (6) ◽  
pp. 702 ◽  
Author(s):  
Dwi Gayatri Nurcahyawati ◽  
Hani Plumeriastuti ◽  
Lilik Maslachah
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Oral Therapy ◽  
White Male ◽  
Male Rats ◽  
Male Rat ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Per Oral ◽  
Tuber Extract

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.  Key words: Eleutherine palmifolia, alloxan, kidney, histhopathology

scholarly journals The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

2019 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Special Treatment ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals Pengaruh Pemberian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Berat, Diameter, Tebal Epitel Epididimis, Motilitas dan Viabilitas Spermatozoa Tikus Putih Jantan (Rattus norvegicus)

2019 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Stephanie Lexy Louis ◽  
Salni Salni ◽  
Sri Nita
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Spermatozoa Motility ◽  
Epithelium Thickness ◽  
Group A ◽  
Ocimum Americanum

<p>Infertility is one of the reproductive problems that may affect both male and female. In a male, the occurrence of reproductive problems may be due to abnormal spermatogenesis processes and anatomic disorder. Kemangi leaves are an aphrodisiac it contains some secondary metabolites like flavonoid, steroid, tannin, and saponin. The objective of the present research was to determine the effect of feeding fractions of kemangi leaves (Ocimum americanum L.) on the epididymis weight, diameter, and epithelial thickness and the spermatozoa motility and viability of white male rat (Rattus norvegicus). The research was an experimental one, with posttest only group design. The research design used a Completely Random Design. The research sample consisted of 24 furrow white male mice Sprague Dawley, 60-74 days old, divided to control group, a fraction of n-hexane, a fraction of ethyl acetate, and a fraction of water-ethanol. The dosage used for the treatment group was 100 mg/kgBB/day, and the control group was given CMC 1%. The result of data analysis by using a One-Way Anova test showed that there were increases in the weight and diameter of the epididymis in the treatment group as compared to that of the control group (p=0,000). Likewise, both epididymis epithelium thickness and spermatozoa viability in the treatment group increased as compared to that of the control group (p=0.001), while spermatozoa motility was p=0,002. It could be concluded that the fractions of kemangi leaves increased the weight, diameter, and epithelium thickness of epididymis and spermatozoa motility and viability in-furrow white male rat Sprague Dawley.<strong></strong></p>

scholarly journals Tempe Flour Increase The Amount of Erythrocytes of White Male Rat (Rattus norvegicus) Wistar Strain Induced by Cigarette Smoke

2019 ◽  
Vol 5 (1) ◽  
pp. 56
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2 ◽  
Hydroxide Radicals ◽  
Group 1

Cigarette smoke was one of the free radical sources for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe because it contains antioxidants substances such as carotene, Vitamin E, isoflavone, and factor II antioxidants. The aim of this study is knowing the influence of giving tempe flour toward the amount of erythrocytes of white male rat (Rattus norvegicus) Wistar strain induced by cigarette smoke. The experiment used a post-test only-control group design method. The samples were white male rats Wistar strain aged of 3 to 4 months as much 30 rats with weight around 180-220 gram. The samples divided into three groups, that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given any treatment, it only got a standard feed, treatment group 1 got treatment and it was induced by cigarette smoke. Furthermore, treatment group 2 got standard feed, it was induced by cigarette smoke and was given tempe. Analysis of data used one-way anova showed the significance p-value = 0,036 that was <α (0,05). The result showed giving tempe flour increase the amounts of erythrocytes of white male rat (Rattus norvegicus) Wistar strain which was induced by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals THE EFFECT OF ADMINISTERING MANGOSTEEN RIND EXTRACT (Garnicia mangostana l) COMPARED WITH GLIMEPIRIDE TO THE BLOOD SUGAR LEVELS OF WHITE MALE RAT (Rattus norwegicus l) INDUCED BY STREPTOZOTOCIN

2017 ◽  
Vol 52 (4) ◽  
pp. 241
Author(s):  
Hasyim As’ari ◽  
Dewi Mutmainnah Mahartini
Keyword(s):  
Treatment Group ◽  
Blood Sugar ◽  
White Male ◽  
Data Distribution ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Sugar Levels

The purpose of this study is to prove the differential effect of administering the mangosteen Rind extract due to lowering the blood sugar levels of Winstar white male rats induced by streptozotosin (STZ). This study used a randomized design of the ramdomized posttest only control group design. The sample consisted of 4 groups with a sample size of 7 animals each group of rats. All samples were prepared STZ induction of diabetes with a single dose of 50 mg/Kg BW through intraperitoneal (IP). K0 (control group; given 1% CMC 1 cc/day), K1 (treatment group 1; given 0,054 mg glimepiride/200 grBW, K2 (treatment group 2; given mangosteen Rind extract 50 mg/Kg BW), K3 (treatment group 3; given the mangosteen Rind extract 100 mg/Kg BW). Timing of therapy in each group was for 7 days. Shapiro-Wilk normality test (a=0.05) in the BW D variable data, pre-post STZ GDP and D GDP. Testing homogeneity used Levene's test. When data distribution was normal and homogenous, it used an ANOVA deferential test. When data distribution was normal and inhomogeneous, it proceed to apply T-test with 2 free samples. The results: 1) There were significant differences between K0 D GDP with K1 (p=0.015), K0 to K2 (p=0.003) and group K0 to K3 (p=0.002), 2) Whereas no difference was shown in the K1 with K2 (p=0.442), K1 to K3 (p=0,401) and K2 to K3 (p=0.878). Conclusion: The administration of mangosteen Rind extract doses of 50 and 100 mg/kg bw/day did not differ in lowering blood sugar levels compared with glimepiride administration of a dose of 0.054 mg/200 grBW rat/day. Mangosteen Rind extract dose of 100 mg/kg bw/day and 50 mg/kg bw/day did not provide a significant difference in lowering blood sugar levels.

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Idola Perdana Sulistyoning Suharto
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Phaleria Macrocarpa ◽  
Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound 

scholarly journals PENGARUH PEMBERIAN EKSTRAK BIJI PINANG (ARECA CATECHU L.) TERHADAP WAKTU PERDARAHAN PASCA EKSTRAKSI GIGI PADA TIKUS JANTAN WISTAR (RATTUS NORVEGICUS L.)

2015 ◽  
Vol 17 (1) ◽  
pp. 129
Author(s):  
Jane Wuisan ◽  
Bernat Hutagalung ◽  
Wellsy Lino
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Bleeding Time ◽  
Male Rats ◽  
Control Group ◽  
Betel Nut ◽  
Areca Catechu ◽  
Time In Treatment ◽  
Teeth Extraction ◽  
Wistar Male Rats

PENGARUH PEMBERIAN EKSTRAK BIJI PINANG (ARECA CATECHU L.) TERHADAP WAKTU PERDARAHAN PASCA EKSTRAKSI GIGI PADA TIKUS JANTAN WISTAR (RATTUS NORVEGICUS L.) ABSTRAK Biji pinang (Areca catechu L.) merupakan salah satu tanaman obat yang dikenal masyarakat dapat menghentikan perdarahan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak biji pinang terhadap waktu perdarahan pasca ekstraksi gigi pada tikus jantan galur Wistar. Pada penelitian ini metode yang digunakan merupakan eksperimental. Ekstrak biji pinang didapatkan dengan cara maserasi dan penguapan menggunakan Rotary vacuum evaporator. Jumlah sampel sebanyak 24 ekor tikus jantan galur Wistar yang dibagi dalam 3 kelompok. Sampel penelitian kelompok perlakuan terdiri dari 8 ekor tikus jantan galur Wistar. Delapan sampel kelompok perlakuan diberi ekstrak biji pinang dosis 13,834 mg/200 g BB. Perhitungan dosis menggunakan tabel faktor konversi. Proses ekstraksi gigi tikus dilakukan setelah 4 jam pemberian ekstrak. Pemeriksaan waktu perdarahan dimulai saat gigi telah terlepas dari soket dengan melihat darah yang keluar sampai mulai terbentuknya bekuan darah pada soket. Perhitungan waktu perdarahan menggunakan stopwatch. Hasil penelitian menunjukkan rerata waktu perdarahan kelompok perlakuan yaitu 0.6125 (menit.detik). Rerata waktu perdarahan kelompok perlakuan tidak jauh berbeda dengan rerata waktu perdarahan kelompok kontrol positif yang diberi transamin yaitu 0.3475 (menit.detik) dan lebih singkat dari kelompok kontrol negatif etanol 96% dengan rerata waktu perdarahan 1.79 (menit.detik). Penelitian yang telah dilakukan menyimpulkan terdapat pengaruh pemberian ekstrak biji pinang (Areca catechu L.) dosis 13,834 mg/200 g BB terhadap waktu perdarahan pasca ekstraksi gigi pada tikus jantan galur Wistar. Kata kunci: Biji pinang, waktu perdarahan, ekstraksi gigi THE EFFECT OF BETEL NUT (Areca catechu L.) EXTRACT ON BLEEDING TIME AFTER EXTRACTION IN WISTAR MALE RATS (Rattus norvegicus L.) ABSTRACT The betel nut (Areca catechu L.) is a medicinal plant known by society to halt bleeding. This study was aimed to know the effect of betel nut extract administration on bleeding time after teeth extraction in Wistar male rats. This study was an experimental study. Betel nut extracts were obtained by maceration and evaporation using Rotary vacuum evaporator. The number of samples were 24 Wistar male rats divided into three groups. The treatment group consisted of 8 Wistar male rats. Eight samples of treatment group were fed with betel nut extract at dosage of 13,834 mg/200 g body weight. Dosages were calculated using conversion factor table. The rats teeth were extracted 4 hours after betel nut extract administration. Evaluation of bleeding time was started from the loss of extracted teeth from socket by observing the shed blood to the formation of blood clots in the socket. The bleeding time was calculated using stopwatch. Study results indicated that average bleeding time in treatment group namely 0.6125 (minute.second). The average bleeding time in treatment group did not differ significantly from average bleeding time in positive control group fed with transamine namely 0.3475 (minute.second), and shorter from negative control group fed with 96% ethanol with average bleeding time of 1.79 (minute.second). This study concluded that there was an effect of betel nut extract (Areca catechu L.) administration at dosage of 13,834 mg/200 g body weight on bleeding time after teeth extraction in Wistar male rats. Keywords: Betel nut, bleeding time, teeth extraction

scholarly journals Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

2020 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ayu Mayang A. P. ◽  
Liliawanti Liliawanti ◽  
Wachjudi Kurnia
Keyword(s):  
Rattus Norvegicus ◽  
White Male ◽  
Brown Seaweed ◽  
Negative Control ◽  
Laboratory Method ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference

<p>Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (<em>Sargassum duplicatum</em>) which can increase hematocrit levels in the blood of white male wistar strain rat (<em>Rattus norvegicus</em>) suffered from anemia in induction NaNO<sub>2</sub>.</p><p>            This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K<sub>0</sub>) without treatment, the positive control (K<sub>1</sub>) be treated anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract and treatment group (K<sub>2</sub>) treated with anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract at a dose of 2,45gr / kgBB. On the 40<sup>th</sup> day, a blood sample was taken, then measured the hematocrit level.</p><p>            The results showed that the mean number of blood hematocrit levels in K<sub>2</sub> was higher than K<sub>1</sub>, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p &gt; 0,05 showed that there were no statistically significant differences between groups of experimental animals.</p><p>            From the results of this research, the conclusion is the administration of brown seaweed extract (<em>Sargassum duplicatum</em>) descriptively increased, but not statistically significant.</p><p> </p><p><strong>Keywords :</strong> Anemia, hematocrit, <em>Sargassum duplicatum</em>, NaNO<sub>2</sub></p>

scholarly journals Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 44
Author(s):  
Valentina Verrel Purnomo ◽  
Agustinus Sareh Arjono Tjandra ◽  
Risma Risma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
White Male ◽  
Test Method ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Standard Diet ◽  
Malus Sylvestris

<p>Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to control the increase of glicemic index. Apple has antioxidant substance such as quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.</p><p>            This research aims to reveal the effect of Manalagi apple vinegar (<em>Malus sylvestris </em>Mill) towards blood glucose level of alloxan induced Wistar white male rats (<em>Rattus norvegicus</em>).</p><p>            This research is experimental laboratory using <em>Experimental </em>methods. The samples are 30 white rats divided into three groups for 31 days, 1) group which only given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for 14 days. The measurement of blood serum glucose level is done on day twenty four using homogenous colorimetric enzymatic test method for all groups.</p><p>            Blood glucose average in group with only given standard diet as negative control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of <em>One-Way Anova </em>significance<em> </em>is 0.229.</p><p>            The conclusion of this research is that Manalagi apple vinegar treatment doesn’t able to decrease the blood glucose level of white Wistar male rats significantly.</p><p> </p><strong>Keywords </strong>: <em>Malus sylvestris </em>Mill, Blood glucose, Alloxan.

scholarly journals 6. Administration of Centella Leaf Extract (Centella asiatica (L.) Urban) for Decreasing cAMP Responsive Element Modulator (CREM) Expression in Testicular Seminiferous Tubule of Male Rats (Rattus norvegicus)

2016 ◽  
Vol 1 (2) ◽  
pp. 31-37
Author(s):  
Susi Darmayanti ◽  
Sri Wahyuni ◽  
Muslim Akmal ◽  
Tongku Nizwan Siregar ◽  
Sugito Sugito
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Male Rats ◽  
Male Rat ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Responsive Element ◽  
Neutral Formalin

The objective of this study was to determine the effect of centella leaf extract administration on decreased of the molecule cAMP responsive element modulator (CREM) expression in the testicular seminiferous tubules of male rats (Rattus norvegicus). Eight rats, aged 3.5 months with 150-250 grams of body weight (BW) were used in this study. All rats were divided randomly into four groups as if K0 as a control group whereas K1, K2, and K3 were given the centella leaf extract with doses 125, 250, and 500 mg / kg body weight respectivelly that given once daily for 30 days. At the end of the treatment, rats were sacrificed and their testes were collected and subsequently fixed in buffered neutral formalin (BNF) 10% as fixative solution for histological preparation. The CREMs expressions were detected using immunohistochemical methods. The results showed that the number of CREM expression in the seminiferous tubules significantly differ (P <0.05) between K0 and the treatment group (K1, K2, and K3). Conclusion, the administration of centella leaf extract with of the dose 125, 250, and 500 mg/kg BW can decreased CREM expression spermatids of testicular seminiferous tubules in male rat.

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