scholarly journals Tempe Flour Increase The Amount of Erythrocytes of White Male Rat (Rattus norvegicus) Wistar Strain Induced by Cigarette Smoke

2019 ◽  
Vol 5 (1) ◽  
pp. 56
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2 ◽  
Hydroxide Radicals ◽  
Group 1

Cigarette smoke was one of the free radical sources for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe because it contains antioxidants substances such as carotene, Vitamin E, isoflavone, and factor II antioxidants. The aim of this study is knowing the influence of giving tempe flour toward the amount of erythrocytes of white male rat (Rattus norvegicus) Wistar strain induced by cigarette smoke. The experiment used a post-test only-control group design method. The samples were white male rats Wistar strain aged of 3 to 4 months as much 30 rats with weight around 180-220 gram. The samples divided into three groups, that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given any treatment, it only got a standard feed, treatment group 1 got treatment and it was induced by cigarette smoke. Furthermore, treatment group 2 got standard feed, it was induced by cigarette smoke and was given tempe. Analysis of data used one-way anova showed the significance p-value = 0,036 that was <α (0,05). The result showed giving tempe flour increase the amounts of erythrocytes of white male rat (Rattus norvegicus) Wistar strain which was induced by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

2019 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Special Treatment ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

scholarly journals Protection of Dayak Onion Tuber Extract (Eleutherine Palmifolia) Against Kidney Histopathological Appearence of Albino Male Rat Strain Wistar which was Induced by Alloxan

2017 ◽  
Vol 3 (6) ◽  
pp. 702 ◽  
Author(s):  
Dwi Gayatri Nurcahyawati ◽  
Hani Plumeriastuti ◽  
Lilik Maslachah
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Oral Therapy ◽  
White Male ◽  
Male Rats ◽  
Male Rat ◽  
Glomerular Sclerosis ◽  
Control Group ◽  
Per Oral ◽  
Tuber Extract

The purpose of this study was to know the effect of Dayak onion tuber extract (Eleutherine palmifolia) given by per oral in lowering levels of histophatology damage kidney of albino male rat (Rattus norvegicus) strain Wistar which was induced by alloxan. Animals which were used in this research were 24 white male rats (Rattus norvegicus) strain Wistar, and then divided into 6 groups. The negative control group K (-) were given with aquabidest and CMC-Na 1% during the therapy period, the positive control group K (+) were given with alloxan 110 mg / kgbw, the group of drug control K (O) were given with alloxan and oral therapy  with metformin 9 mg / 200g bw / day, the treatment group 1 (P1) were given with alloxan and per oral therapy with extract of  Dayak onion tuber 100 mg / kgbw, the treatment group 2 (P2) were given with alloxan and per oral therapy with extract of Dayak onion tuber 200 mg / kgbw and the treatment group 3 (P3) were given with alloxan and per oral therapy with extract of Dayak onion tuber 400 mg / kgbw. The therapy was given for 14 days, then the animals were sacrificed with ketamine and then its kidney was taken for examination of hisphatology in kidney. Observations based on their depiction of renal histopathology tubular degeneration and necrosis, glomerular necrosis, intestitial infiltration and glomerular sclerosis. Data obtained from the scoring of histopathological appearence albino rat kidneys were analyzed by test Kruskal-Wallis and if there is a real difference followed by Mann-Whitney test using SPSS 20.0 for windows. The results showed that the extract of Dayak onion tuber 400 mg/ kgbw  can reduce the degree of kidney damage in albino male rat exposed to alloxan significantly.  Key words: Eleutherine palmifolia, alloxan, kidney, histhopathology

scholarly journals The Effect of Propofol on Onset and Sedation in Diabetic Mellitus Rat (Rattus norvegicus)

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Erwin Erwin ◽  
Rusli Rusli ◽  
Zuraidawati Zuraidawati ◽  
Fadillah Irwansyah
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Diabetic Mellitus ◽  
Wistar Strain ◽  
Female Wistar Rats ◽  
Ad Libitum ◽  
Group 2 ◽  
Group 1

This research aimed to examine the time of onset and sedation on diabetic mellitus (DM) rat (Rattus norvegicus) by propofol. This study used 8 female wistar rats 2-3 months old and 150-200 grams of bodyweight, fed with standard feed and water ad libitum. Samples were divided into two groups. Group 1 (KI) as a control group was injected intraperitoneally with aloxan solvent and group 2 (KII) was injected intraperitoneally with 150 mg/kg bodyweight aloxan. Blood glucose was checked 10 days after aloxan injection, DM was categorized if the blood glucose over 150 mg/dl. All rats were injected intravenously with propofol 1% with the dose of 10 mg/kg bodyweight. Onset and sedation time was counted using stopwatch after propofol injection. The result showed that mean (±SD) of onset on KI and KII were 1.39±0.49 and 8.64±1.23 seconds, consecutively and they were significantly different at P0.01. Whilst mean (±SD) of sedation KI and KII were 12.12±1.47 minutes and 7.62±1.61 minutes, respectively. The onset and sedation time of KI was significantly different from KII P0.01. The conclusion of this research was DM affects time of onset and sedation when wistar strain rats were anesthetized by 1% propofol.Key words: rats, diabetic mellitus, propofol, onset, sedation

scholarly journals Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons

2018 ◽  
Vol 25 (3) ◽  
pp. 97
Author(s):  
Eka Nasrur Maulana ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati ◽  
Windhu Purnomo
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Apoptotic Index ◽  
Assay Method ◽  
Optimal Time ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control group's neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found  the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.

Pengaruh Pemberian Ekstrak Biji Petai (Parkia speciosa) Terhadap Gambaran Histopatologi Ginjal Bagian Tubulus Proksimal Pada Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Paracetamol

2018 ◽  
Vol 15 (2) ◽  
pp. 233
Author(s):  
AVINDA DEVIANA
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Positive Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
Standard Diet ◽  
Post Test ◽  
Inflammatory Drugs ◽  
Group 2 ◽  
Group 1

<p>Paracetamol, which is one of the NSAIDs (<em>Non Steroidal Anti Inflammatory Drugs</em>) is widely used by the wider community as a therapeutic and pain-reducing therapy. In addition to its many uses, paracetamol can cause a variety of serious toxicities, including nephrotoxicity. Bitter bean extract (<em>Parkia speciosa</em>) has a flavonoid compound which is an antioxidant. This was a laboratory experimental study with <em>post-test only control</em> <em>group </em>design using 24 rats divided into 3 groups. Positive control group fed with standard diet, aquades and bitter bean extract (<em>Parkia speciosa</em>); each rat in the treatment group 1 was induced with paracetamol 9 mg / day orally; and each rat in the treatment group 2 was given bitter bean extract (<em>Parkia speciosa</em>) 7,2 mg / day induced paracetamol 9 mg / day after 45 min. The kidneys were taken by surgery on the 15th day and microscopically examined. The data were analyzed using IBM SPSS version 23 with Kruskal-Wallis method.</p>

scholarly journals Pengaruh Pemberian Fraksi Daun Kemangi (Ocimum americanum L.) terhadap Berat, Diameter, Tebal Epitel Epididimis, Motilitas dan Viabilitas Spermatozoa Tikus Putih Jantan (Rattus norvegicus)

2019 ◽  
Vol 10 (1) ◽  
pp. 25
Author(s):  
Stephanie Lexy Louis ◽  
Salni Salni ◽  
Sri Nita
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Spermatozoa Motility ◽  
Epithelium Thickness ◽  
Group A ◽  
Ocimum Americanum

<p>Infertility is one of the reproductive problems that may affect both male and female. In a male, the occurrence of reproductive problems may be due to abnormal spermatogenesis processes and anatomic disorder. Kemangi leaves are an aphrodisiac it contains some secondary metabolites like flavonoid, steroid, tannin, and saponin. The objective of the present research was to determine the effect of feeding fractions of kemangi leaves (Ocimum americanum L.) on the epididymis weight, diameter, and epithelial thickness and the spermatozoa motility and viability of white male rat (Rattus norvegicus). The research was an experimental one, with posttest only group design. The research design used a Completely Random Design. The research sample consisted of 24 furrow white male mice Sprague Dawley, 60-74 days old, divided to control group, a fraction of n-hexane, a fraction of ethyl acetate, and a fraction of water-ethanol. The dosage used for the treatment group was 100 mg/kgBB/day, and the control group was given CMC 1%. The result of data analysis by using a One-Way Anova test showed that there were increases in the weight and diameter of the epididymis in the treatment group as compared to that of the control group (p=0,000). Likewise, both epididymis epithelium thickness and spermatozoa viability in the treatment group increased as compared to that of the control group (p=0.001), while spermatozoa motility was p=0,002. It could be concluded that the fractions of kemangi leaves increased the weight, diameter, and epithelium thickness of epididymis and spermatozoa motility and viability in-furrow white male rat Sprague Dawley.<strong></strong></p>

scholarly journals Pengaruh Vitamin D3 Terhadap Kadar Hemoglobin Tikus Wistar yang Dipapar Asap Rokok

2019 ◽  
Vol 1 (1) ◽  
pp. 22-28
Author(s):  
Hetti Rusmini ◽  
Dita Fitriani ◽  
Dessy Hermawan ◽  
Diah Adelia Emilda
Keyword(s):  
Cigarette Smoke ◽  
Erythrocyte Membrane ◽  
Vitamin D3 ◽  
Rattus Norvegicus ◽  
Not Given ◽  
White Rats ◽  
Wistar Strain ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Hemoglobin is a tetrametric erythrocyte protein that carries O2 to the tissues and returns CO2 and protons to the lungs. Cigarette smoke is proven that could reduce hemoglobin levels through oxidative stress which causes the erythrocyte membrane to be easily lysed. The lysis erythrocyte membrane causes hemoglobin levels to be low. Vitamin D is included in natural antioxidants which have a neuroprotective tendency through antioxidative mechanisms. To determine the effect of giving vitamin D3 on hemoglobin levels in white rats (Rattus norvegicus) male Wistar strain after being exposed by cigarette smoke. This type of pure experimental research (tue-experiment) used pre and post with control group design. Samples were white rats (Rattus norvegicus) male Wistar strain aged 10-12 weeks with the weight of around 150-200 grams in a total of 24 individuals. Samples were divided into four groups including K1 which is not exposed by cigarette smoke and not given of vitamin D3, K2 is a group exposed by cigarette smoke but not given of vitamin D3, K3 is a group that is not exposed by cigarette smoke but given of vitamin D3 a dose of 0.2 µgr /head, P1 is a group exposed by cigarette smoke and given a vitamin D3 dose of 0.2 µgr /head. The test of paired t-test showed a significant difference p <0.05 in the K2 group with p = 0.044, the K3 group with p = 0.013, and P1 group with p = 0.037. Whereas in group 1 there was no significant difference p = 0.932. The One-way Anova test obtained results with a value of p = 0.027 (p <0.05) which means that there were significant differences between groups. The Post Hoc LSD test showed a significant difference in group 1 whit group 2 p = 0.025 and in group 2 with group 3 p = 0.012.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals Pengaruh pemberian ekstrak pasak bumi (Eurycoma longifolia Jack) terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Bill C. A. Bogar ◽  
Lydia Tendean ◽  
Grace L. A. Turalaki
Keyword(s):  
Rattus Norvegicus ◽  
Control Group ◽  
Adult Rats ◽  
Beneficial Effects ◽  
Eurycoma Longifolia ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Eurycoma Longifolia Jack ◽  
Group 2 ◽  
Group 1

Abstract: Infertility is an inability of couples to reproduce after carrying out sexual intercourses for at least a year without using any contraceptives. There are 10-15% couples worldwide who experience infertility problems and almost one half of them is on men. Spermatozoa qualities including concentration, motility, and morphology are used as an indicator for men’s fertility. The utilization of herbal medicine (i.e. Eurycoma longifolia Jack extracts) is now being an alternative way to improve fertility among the community. This study was carried out to find the effects of Eurycoma longifolia Jack on spermatozoa qualities. This experimental study was conducted to nine male wistar rats (Rattus norvegicus) weighing from 200-250 grams, aging from 12-15 months. These nine adult rats were divided into 3 groups of 3 rats each. 400 and 600 mg/kg/day of Eurycoma longifolia Jack extracts were orally administrated to group 1 and 2 respectively, while group 3 were treated as control group. After 50 days, the animals of group 1, 2 and 3 were sacrificed. As a result, there is an improvement in qualities of spermatozoa which are statistically significant (p<0,05) in group 2. Thus, provide the beneficial effects of 600mg/kg Eurycoma longifolia Jack extracts on the qualities of spermatozoa.Keywords: pasak bumi, spermatozoaAbstrak: Infertilitas adalah ketidakmampuan pasangan untuk dapat hamil setelah satu tahun berhubungan intim tanpa menggunakan kontrasepsi. Ada sekitar 10 – 15 % pasangan mengalami masalah infertilitas dan hampir setengahnya masalah ada pada pria. Kualitas spermatozoa meliputi konsentrasi, motilitas dan morfologi spermatozoa merupakan salah satu indikator fertilitas pada pria. Penggunaan tanaman herbal atau yang lebih dikenal jamu telah menjadi pengobatan alternatif di masyarakat. Tanaman herbal yang dimaksud adalah pasak bumi. Penelitian ini bertujuan untuk mengetahui efek dari esktrak pasak bumi (Eurycoma longifolia Jack) terhadap kualitas spermatozoa. Penelitian ini menggunakan 9 ekor tikus wistar jantan (Rattus norvegicus) dengan berat badan 200-250 g berumur 12-15 bulan. Sembilan ekor wistar dibagi menjadi tiga kelompok, dan satu kelompok terdiri dari tiga ekor tikus wistar. Penelitian ini dilaksankan selama 50 hari. Esktrak pasak bumi (Eurycoma longifolia Jack) diberikan dengan dosis 400mg/kgBB per hari pada perlakuan 1 dan dosis 600 mg/kgBB per hari pada perlakuan 2. Setelah 50 hari hewan coba pada kelompok kontrol, perlakuan 1 dan perlakuan 2 diterminasi. Hasil penelitian memperlihatkan terjadi peningkatan kualitas spermatozoa yang signifikan secara statistik (p<0,05) pada perlakuan 2 terhadap kelompok kontrol. Hasil tersebut menunjukan bahwa Esktrak pasak bumi (Eurycoma longifolia Jack) pada dosis 600 mg/kgBB dapat meningkatkan kualitas spermatozoa.Kata kunci: pasak bumi, spermatozoa

scholarly journals A field trial evaluation of the prophylactic efficacy of amitraz-impregnated collars against canine babesiosis (Babesia canis rossi) in South Africa

2007 ◽  
Vol 78 (2) ◽  
Author(s):  
R.D. Last ◽  
J.D. Hill ◽  
P.T. Matjila ◽  
C.A. Reme
Keyword(s):  
South Africa ◽  
Treatment Group ◽  
Geographical Area ◽  
Tick Control ◽  
Domestic Dog ◽  
Control Group ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Group 2 ◽  
Group 1

South African canine babesiosis caused by Babesia canis rossi is a common clinical disease in dogs in South Africa and remains a significant cause of domestic dog mortality. To determine whether tick-repellent, 9% amitraz-impregnated tick collars (Preventic-Virbac) could prevent tick-borne exposure to B. canis rossi, 50 dogs were assigned to two groups. Group 1 (20 dogs), polymerase chain reaction (PCR)- and reverse line blot (RLB)-negative for B. canis rossi, were fitted with amitraz collars and blood samples collected monthly , over a 6-month period, and analysed for B. canis rossi. Group 2 (30 dogs) included 5 dogs selected on a month-by-month basis from a population of dogs from the same geographical area as the group 1 dogs, but with no history of previous tick control, which were blood-sampled together with the treatment group and analysed for B. canis rossi by PCR and RLB, to serve as the control group. Eight of the 30 control dogs (26.6 %) were PCR / RLB positive for B. canis rossi, indicating high pathogen exposure during the trial period. All twenty of the treatment group dogs remained negative for B. canis rossi throughout the 6 months of the trial. These results suggest that the use of amitraz-impregnated collars had a significant effect on reducing infection with B. canis rossi.

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