EFFECT OF POLYPHENOLIC COMPOUNDS ISOLATED FROM CARTHAMUS TINCTORIUS AND CALENDULA OFFICINALIS L., ON FUNCTIONAL ACTIVITY OF IMMUNE CELLS UNDER CONDITIONS OF CYTOSTATIC IMMUNOSUPPRESSION

The purpose of the study – to study the effect of polyphenolic compounds extracted from the flowers of safflower oil and calendula, the functional activity of immune cells in cytotoxic immune suppression.Conventional methods determined the total number of splenocytes, relative (%) and absolute (106), the number of antibody-forming cells (AFC) in the spleen of mice by local hemolysis by Cunningham.Evaluated the effect of compounds of natural origin on the cellular immune response in the delayed-type hypersensitivity (DTH). Phagocytic activity of peritoneal macrophages was determined by the method based on the intensity of their capture ink particles. We studied the functional activity of peritoneal macrophages by NBT test (spontaneous and stimulated). Studies were conducted on male mice Category 1 (conventional linear mouse) line CBA/CaLac aged 2–2.5 months, weighing 20–22 g. After the introduction mice line CBA/CaLac of polyphenolic compounds derived from flowers of Carthamus tinctorius and flowers of Calendula officinalis L. during the 5-day course in dose 50 mg/kg was observed stimulation of the humoral immune response (total number of splenocytes, the number of antibodies in the spleen cells) and the functional activity of macrophages and Immunomodulating effect on the humoral immunity and the functional activity of macrophages after a single injection of cyclophosphamide in dose 250 mg/kg. Immunotropic activity of polyphenolic compounds is higher than that those of the reference product of tincture of Echinacea purpurea.

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Choleretic activity of dry extracts of Carthamus tinctorius L., Tagetes erecta L.Calendula officinalis L.

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-175-3-76-79 ◽

2020 ◽

pp. 76-79

Author(s):

S. M. Nikolaev ◽

N. S. Badmaev ◽

Z. G. Sambueva ◽

D. N. Olennikov ◽

N. I. Kashchenko

Keyword(s):

Water Solution ◽

White Male ◽

Carthamus Tinctorius ◽

Biologically Active ◽

Male Rats ◽

Tagetes Erecta ◽

Calendula Officinalis ◽

Tagetes Erecta L ◽

Calendula Officinalis L ◽

Choleretic Effect

The aim of the present work was to estimate the choleretic effect of dry extracts derived from Carthamus tinctotius L., Calendula officinalis L. and Tagetes erecta L.Materials and methods: The experiments were carried out on white male rats weighing 180–230 g. The extracts in a single dose were introduced into the duodenum of the rats at the doses of 50, 100 and 200 mg/kg in the form of water solution.Results: The results of the experiments have shown that the extracts from Calendula officinalis and Carthamus tinctorius have the marked choleretic effect due to the presence of biologically active substances, predominantly fl avonoids.

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TURMERIC EXTRACT IN CORRECTION OF NERVOUS AND IMMUNE SYSTEMS FUNCTIONAL ACTIVITY PARAMETERS IN EXPERIMENTAL ALCOHOLISM

Medical academic journal ◽

10.17816/maj191s1215-217 ◽

2019 ◽

Vol 19 (1S) ◽

pp. 215-217

Author(s):

E V Markova ◽

I A Goldina ◽

B G Goldin ◽

M A Knyazheva ◽

I V Savkin

Keyword(s):

Immune Response ◽

Functional Activity ◽

Cellular Immune Response ◽

Experimental Models ◽

Ethanol Solution ◽

Delayed Type Hypersensitivity ◽

Experimental Alcoholism ◽

Immune Systems ◽

Turmeric Extract ◽

Cellular Immune

The purpose of this work was to study the effect of turmeric extract on behavior indicators, the severity of the cellular immune response in animals in a state of experimental alcoholism. Experimental models: mouse males (CBAxC57Bl/6)F1 three months of age (n = 60). Alcohol dependence in experimental animals was formed by the method of 6-month soldering with a 10% ethanol solution. In the control groups, the animals received per os water or 10% ethanol solution, in the experimental group - an extract of turmeric powder in a solution of ethanol. Mice behavior was assessed in the “open field” test. The severity of the cellular immune response to sheep erythrocytes was assessed by the intensity of the development of a delayed-type hypersensitivity reaction.It was found that the use of turmeric extract against the background of taking ethanol solution in animals with experimental alcoholism leads to the stimulation of behavior and the increase of the cellular immune response to the level characteristic of healthy animals of the corresponding age.Results indicates the protective effect of turmeric on a number of parameters of the functional activity of the nervous and immune systems during chronic ethanol intoxication.

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Effect of vitamin D on humoral and cell-mediated immune response and functional activity of peritoneal macrophages

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf02764379 ◽

1997 ◽

Vol 123 (1) ◽

pp. 55-56

Author(s):

K. D. Pletsityi ◽

T. V. Davydova ◽

V. G. Fomina ◽

E. V. Nikushkin

Keyword(s):

Vitamin D ◽

Immune Response ◽

Functional Activity ◽

Peritoneal Macrophages ◽

Cell Mediated Immune Response

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Measuring host immune response status by simultaneous quantitative measurement of activity of signal transduction pathways that coordinate functional activity of immune cells from innate and adaptive immune system

10.1101/2021.10.06.463309 ◽

2021 ◽

Author(s):

Wilbert Bouwman ◽

Wim Verhaegh ◽

Arie van Doorn ◽

Anja van de Stolpe

Keyword(s):

Immune Response ◽

Immune Cells ◽

Functional Activity ◽

Immune Cell ◽

Adaptive Immune Response ◽

Cell Types ◽

Activity Profile ◽

Pathway Activity ◽

Adaptive Immune

Abstract For many diseases, including cancer, viral infections such as COVID-19, bacterial infections, and auto-immune diseases, the immune response is a major determinant of progression, response to therapy, and clinical outcome. Innate and adaptive immune response are controlled by coordinated activity of multiple immune cell types. The functional activity state of immune cells is determined by cellular signal transduction pathways (STPs). A novel mRNA-based signaling pathway assay platform has been developed to quantitatively measure relevant STP activities in all types of immune cells and mixed immune cell samples for experimental and diagnostic purposes. We generated a STP activity profile, termed Immune-Pathway Activity Profile (I-PAP), for a variety of immune cell types in resting and activated state, and provide a first example for use in patient samples. Methods. The technology to measure STP activity has been described for androgen and estrogen receptor, PI3K, MAPK, TGFB;, Notch, NFkB, JAK-STAT1/2, and JAK-STAT3 pathways. STP activity was measured on Affymetrix expression microarray data from preclinical studies containing public data from different types of immune cells, resting/naive or immune-activated in vitro, to establish I-PAPs. Subsequently data from a clinical study on rheumatoid arthritis were analyzed. Results. I-PAPs of naive/resting and immune-activated CD4+ and CD8+ T cells, T helper cells, B cells, NK cells, monocytes, macrophages, and dendritic cells were established and in agreement with known experimental immunobiology. In whole blood samples of rheumatoid arthritis patients TGFβ pathway activity was increased; JAK-STAT3 pathway activity was selectively increased in female patients. In naive CD4+ Tregs TGFB; pathway activity was increased, while in memory T effector cells JAK-STAT3 pathway activity tended to increase, suggesting that these immune cell types contributed to whole blood analysis results. Conclusion. STP assay technology (currently being converted to qPCR-based assays) makes it possible to directly measure functional activity of cells of the innate and adaptive immune response enabling quantitative assessment of the immune response of an individual patient. Envisioned utility lies in (1) prediction and monitoring of response to immunomodulatory treatments for a variety of immune-mediated diseases, including RA; (2) uncovering novel treatment targets; (3) improvement and standardization of in vitro immunology research and drug development.

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A Proinflammatory Effect of the β-Glucan from Pleurotus cornucopiae Mushroom on Macrophage Action

Mediators of Inflammation ◽

10.1155/2017/8402405 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Ken-ichiro Minato ◽

Akihiro Ohara ◽

Masashi Mizuno

Keyword(s):

Vitamin D ◽

Immune Response ◽

Immune Cells ◽

Treated Group ◽

Peritoneal Macrophages ◽

Innate Immune ◽

Control Group ◽

Innate Immune Cells ◽

Proinflammatory Effect ◽

And Control

PCPS from P. citrinopileatus mushroom extract is a β-1,6-glucan possessing a proinflammatory effect on innate immune cells. The PCPS stimulated THP-1 macrophages to secrete significant levels of TNF. Moreover, the mRNA expressions of TNF and IL-1β were significantly enhanced by PCPS treatment. However, the PCPS did not induce to express both IL-12 and IL-10 mRNA in the macrophages. Next, the P. cornucopiae extract (containing mainly PCPS) treatment against mice showed significant increases in TNF and IL-1β mRNA expressions in the peritoneal macrophages of them. In this study, the expression levels of IFNγ mRNA in the spleen were almost the same between the extract- (PCPS-) treated group and control group. However, the expression of IL-4 mRNA showed a lower level in the extract-treated group than that in the control. Our results suggested that the PCPS could induce proinflammatory action in the immune response. In addition, the proinflammatory effect of the PCPS on THP-1 was enhanced by 5′-GMP-Na, while it was reduced by vitamin D2. These two compounds are majorly contained in the P. citrinopileatus mushroom. Therefore, these results suggested that the P. citrinopileatus mushroom might contain other immune regulative compounds, such as vitamin D2, as well as PCPS.

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