scholarly journals Effect of Condensed Tannin Supplementation through a Ficus benghalensis Tree Leaves on Erythrocytic Antioxidant Status and Gastrointestinal Nematodes in Kids

Author(s):  
P.M. Tabhani ◽  
M. Choubey ◽  
V.R. Patel ◽  
K.K. Sorathiya ◽  
A.B. Sorathiya ◽  
...  
2019 ◽  
pp. 01-09
Author(s):  
Suarez VH ◽  
Olmos LH ◽  
Martinez GM ◽  
Sandoval GV ◽  
Alfaro EJ ◽  
...  

In order to evaluate condensed tannins (CT) effect on dairy goat gastrointestinal nematodes (GIN) under a grazing and confined systems, two experiments (E1 and E2) were made. On both, goats were kept in two groups of 10 animals each. Groups were: control, without treatment (CG) and CT supplemented (CTG) daily with 25 g (1,25%) in E1 and 75 g (2%) in E2. During the E1, feeding was based on Lucerne (Medicago sativa) grazing and supplementation with 500 g of corn grain and during the E2, stabled milking goats ate 500 g of corn grain and ad libitum lucerne hay. Biweekly, faecal egg counts (FEC) and coprocultures were made. In addition, FAMACHA© technique, body condition score (BCSS) and ingested food were recorded each two weeks during the 90 days (E1) and 76 (E2) days of trial. The FEC values in most of the study were low and no significant differences (p<0.78) were detected between groups. Mean FEC were 534 (CTG) and 357 (CG) during E1 and 509 (CTG) and 484 (CG) during E2. The predominant NGI genera were Trichostrongylussp. (48.1%), Haemonchus sp. (39.9%) and Teladorsagia sp. (12.0%). FAMACHA© scores did not show significant (p<0.41) differences between groups, but the CG (median=3) showed a BCS significantly (p<0.05) higher than those of CTG (median=2.5). The average milk production did not present statistically significant differences between groups (p<0.57), between sampling dates (p<0.23) and group x sampling dates (p<0.65). Under the conditions of these studies 25g or 75 g of CT in the diet had no anthelmintic or productive effects. Keywords: Gastrointestinal nematode; Dairy goat; Condensed tannin; Production


2018 ◽  
Vol 13 (1) ◽  
pp. 269-278 ◽  
Author(s):  
Mustafa Olfaz ◽  
Unal Kilic ◽  
Mustafa Boga ◽  
Abdiwali Mohamoud Abdi

AbstractThis study was conducted to determine the potential nutritive value andin vitrogas production (IVGP) parameters ofOlea europaea L. (Olive = OL),Morus alba L. (Mulberry = ML) andCitrus aurantium L. (Sour orange = SOL) tree leaves. Hohenheim gas test was used to determine thein vitrogas productions of the leaves. The gas production of samples over time was recorded for 3, 6, 9, 12, 24, 48, 72 and 96 h after incubation. Completely Randomized Design was used to compare gas production, and gas production kinetics of samples. The findings of the present study suggested that there were differences among the tree leaves in terms of crude protein, NDF,in vitrogas productions, organic matter digestibility (OMD), metabolisable energy (ME), net energy lactation (NEL) and relative feed values (RFV) (P<0.01). ML had the highest condensed tannin contents (P<0.05),in vitrogas production (IVGP), OMD and energy values (P<0.01). SOL had highest RFV values. OL showed the lowest IVGP when compared to SOL and ML. Low NDF and ADF contents of SOL would probably increase the voluntary intake, digestibility and relative feed values of these leaves by ruminants. In conclusion, it was determined that OL, ML and SOL used in the study have lowin vitrogas production and can be utilized as alternative roughage feed in ruminants. However, it is recommended that the results obtained from this research should be tested inin vivostudies.


2019 ◽  
Vol 948 ◽  
pp. 78-84
Author(s):  
Awistaros Angger Sakti ◽  
Kustantinah ◽  
Raden Wisnu Nurcahyo ◽  
Lovy Perdani ◽  
Melisa Ekaningrum

Condensed Tannin (CT) are secondary metabolites of the plant that synthesized along the phenylpropanoid pathway. It is known to suppress CH4emission in the rumen through protozoal defaunation as well as direct effects on methanogen bacteria. Recent studies have been reported to have anthelmintics activity to overcome gastrointestinal nematodes, appertain to theHaemonchus contortus. To obtain merits of CT, it can be applied in flour or infusion form. Infusion considered as a convenient alternative of CT application. Evaluation method to produce optimum levels of CT needs to be done for the merits of CT obtained optimally. This study aimed to evaluate the effect of leaf maturity, maceration time, and centrifuge force on CT levels produced in infusion leaf as a feed additive. Mature and immature leaves selected from Morindacitrifolia(CF), Muntingiacalabura(CA), Azadirachta indica(AZ), Hibiscus rosa sinensis (RS) and Hibiscus tiliaceus (HT).The results of this research showed that leaf age had a significant effect on CT, except on AZ and RS. Normally, CT content of immature leaf is higher than the mature leaf. CA has highest levels of CT, can be predicted because of lots of glandular trichomes in their mature leaves as a place to store secondary metabolite compounds. Optimum levels of CT from maceration occurring at 2h of immersion except on HTthat requiring 4h of immersion. Meanwhile, 372 g is the most effective centrifuge force in producing optimum CT, except in CA which requires 2.318 g. Based on this study, it can be concluded that leaf age, maceration time, and centrifuge force have a significant influence in producing an infusion with maximum CTat each stage of extraction. It is important for infusion production to contain CT in optimal amounts. This research is expected to be the first step in providing multifunctional feed additive for livestock.


1995 ◽  
Vol 1995 ◽  
pp. 71-71
Author(s):  
C J Powell ◽  
C E Lascano ◽  
D L Romney ◽  
M Gill

The potential benefits of mixing leaves containing high and low levels of condensed tannin (CT) on N utilisation in sheep was studied at the Centro Internacional de Agricultura Tropical (CIAT), Colombia.Supplements of Cratylia argentea (Ca) (tannin free), replaced with 0, 25, 50 or 100% Flemingia macrophylla (Fm) (25.1 g CT/kg DM) were fed as 40% of total ration DM (85g/M0·75), the remainder coming from Brachiaria dictyoneura (Bd). Treatments were offered in a replicated 4x4 latin square to eight male African Hair sheep (mean liveweight 23 (s.e. 1.5) kg), fistulated at the rumen and proximal duodenum (T-piece). Total collections of urine and faeces were used to estimate apparent N retention. Ammonia concentration was measured in rumen liquor samples taken hourly over 24 hours, while duodenal flows were estimated from spot samples using Ytterbium chloride as a single phase marker.


2011 ◽  
Vol 10 (2) ◽  
pp. 77
Author(s):  
H. M. Al-Ramahi

The study was carried out in Babel province, where three sheep farms evaluated for albendazole, levamisol and ivermectin resistance in nematodes by in vivo and in vitro techniques as well as to demonstrate the efficacy of commercial tannin-rich solution on the resistant genera.According to low confidence interval limit of 95% fecal egg count reduction FECR ,In farm I, the animal which treated with levamisol and ivermectin considered as suspected resistant, while the animals which treated with albendazole showed resistance to it. In farm II ,the animal which treated with levamisol and albendazole were considered as suspected resistant ,while no FECR% <95 % was recorded in animals which treated with ivermectin ,and in the same manner ,the animals of farm III which treated with levamisol and albendazole showed suspected resistance and resistance respectively, and no resistance to ivermectin was recorded in animals which treated with it. Also, the results revealed that the combination between in vitro (EHA) and in vivo (FECRT) necessary to confirm the assessment of resistance against benzimedazoles. The most common genus identified from pre treatment feces were Haemonchus ,Trichostrongylus and Cooperia ,while Haemonchus was the only identified genus which demonstrated from post treatment fecal culture.The compared of in vitro effect of condensed tannin CT and thiabendazol revealed that both thiabendazole and CT induced significant egg hatching inhibition in a dose-dependent manner. The thiabendazole required a maximum of 0.5mg/ml, whereas, the CT required a maximum concentration of 2 mg/ml, to induce 100% egg hatch inhibition. The adult worm motility assay showed that the thiabendazole kill all worms at the concentration of 0.25 mg/ml, while CT was showed insignificant killing even in maximum concentration.


2020 ◽  
Vol 41 (2) ◽  
pp. 517-530
Author(s):  
Danilo Rodrigues Barros Brito ◽  
◽  
Livio Martins Costa Júnior ◽  
João Luis Garcia ◽  
Daniel Praseres Chaves ◽  
...  

Author(s):  
Hasan Haci Yeter ◽  
Berfu Korucu ◽  
Elif Burcu Bali ◽  
Ulver Derici

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.


2017 ◽  
Vol 87 (3-4) ◽  
pp. 179-190
Author(s):  
Amel Kanane ◽  
Fayrouz Rouaki ◽  
Mohamed Brahim Errahmani ◽  
Abdenour Laraba ◽  
Hayet Mesbah ◽  
...  

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.


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