scholarly journals Annual variations of alpha-amylase and invertases activities in buds and internodes of grapevines and their relation with carbohydrates and abscisic acid contents

OENO One ◽  
2005 ◽  
Vol 39 (3) ◽  
pp. 129
Author(s):  
Tayeb Koussa ◽  
Lalla Aicha Rifai ◽  
Monique Cherrad
Keyword(s):  
Abscisic Acid ◽  
Annual Cycle ◽  
Amylase Activity ◽  
Starch Content ◽  
Physiological State ◽  
Starch Accumulation ◽  
Soluble Carbohydrates ◽  
Vitis Vinifera L ◽  
Bud Burst ◽  
Aba Content

<p style="text-align: justify;">The concentration of starch, soluble carbohydrates, free abscisic acid (ABA) and activities of a-amylases and invertases (the acid and the alkaline form) were investigated in buds and internodes of Vitis vinifera L. cv. Merlot during annual cycle. The levels of starch increased in the two organs from middle august to middle of November and the decreased until middle February. These variations seem to be controlled by a-amylase which activity was low during periods of starch accumulation and high when starch contents decreased. As the bud burst draws near (since the middle of February), starch accumulated in internodes and buds. However at the same time of the activity of a-amylase which activity also increased. Starch contents could be controlled by ABA which is known to reduce a-amylase activity. Indeed in internodes ABA content was high when a-amylase activity was low. This was also the case in the buds but after break dormancy phase. This difference of comportment of a-amylase towards ABA in the buds during the annual cycle could be in relation with the change of the physiological state of buds induced by the winter chilling.</p><p style="text-align: justify;">The invertases activities in buds and internodes changed inversely to the sucrose and raffinose contents during all the annual cycle suggesting that the levels of these sugars were controlled by invertases, highly by the acid form and slightly by the alkaline form. Nevertheless, in the buds, when alkaline activity of invertase was high, sucrose content was maximal. These invertases seem also implicated, with a-amylase, in the development of starch content particularly when starch was accumulated by glucose providing. In the case of buds, invertases activities appeared to change in the same sense of ABA content but not during all the cycle. This suggested that this growth regulator may not be crucial for the control of invertases activities.</p>

scholarly journals Variations of abscisic acid contents in various organs of grapevine infected by the eutypa dieback fungus, Eutypa Lata

OENO One ◽  
2006 ◽  
Vol 40 (4) ◽  
pp. 187
Author(s):  
Tayeb Koussa ◽  
Lalla Aicha Rifai ◽  
Bernadette Dubos ◽  
Michel Broquedis
Keyword(s):  
Abscisic Acid ◽  
Growth Regulator ◽  
Growth Cycle ◽  
Vitis Vinifera L ◽  
Flower Buds ◽  
Eutypa Lata ◽  
Eutypa Dieback ◽  
Growth Increase ◽  
Aba Content ◽  
Medium Culture

<p style="text-align: justify;">In grapevine, eutypa dieback is a disease induced by ascomyceta fungus, Eutypa lata. At present, eutypa dieback is considered as the most serious deterioration disease of grapevine. The external symptoms of this disease are most conspicuous during the first months of the annual growth cycle and include dwarfed shoots with smaller and necrotic leaves and unfavourable development of grapes. All these symptoms were still suggested an hormonal perturbation in grapevine organs. The aim of this work was to determine the effects of eutypa dieback on abscisic acid contents in flower buds, flowers, young berries, leaves and internodes of grapevine. This study was also performed to determine the relationship between this growth regulator and disease development. The study was performed using organs (1) from healthy vines, (2) healthy appearing organs from vines with one symptomless arm and one diseased arm, and (3) moderately and (4) strongly diseased organs. From flowers buds separated to bunch of grapes closed stages, free abscisic acid (ABA) and the glucose esters of this acid (ABA-GE) were analysed in Vitis vinifera L. cv. Cabernet Sauvignon which is sensitive to E. lata. In the healthy appearing organs, growth and development as well as water and ABA contents were not changed by eutypa dieback fungi. Whereas eutypa dieback induced a decrease of water content and the rate of growth increase in diseased organs. During all the period studied in leaves and since fruit set stage in the internodes, the ABA and ABA-GE contents were increased by eutypa dieback proportionally to symptoms. The ABA levels increase in leaves and internodes were not seem to be produced by ABA-GE hydrolysis since increase in ABA-GE levels were also observed in response to eutypa dieback. The maximum of ABA characterising grapevine flowers was delayed by eutypa dieback and their level was reduced with increasing symptoms severity. In flower buds, flowers and young berries, this disease induced a decrease of ABA-GE contents during all period studied proportionally to symptoms severity. The mycelium of two E. lata isolates cultured in Errikson and Petersson medium culture can synthesize ABA. A part of this growth regulator was diffused in medium culture. It was suggested that increase of ABA levels in diseased organs must have many origins: perturbation of (1) ABA-GE translation, (2) synthesis of ABA by grapevine caused by water stress and (3) synthesis of ABA by the fungi. The possible relationship between increased ABA content in the diseased organs and expression of eutypa dieback symptoms is discussed.</p>

Changes in Carbohydrate Content during Wheat Maturation—What is Measured by near Infrared Spectroscopy?

2005 ◽  
Vol 13 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Szilveszter Gergely ◽  
András Salgó
Keyword(s):  
Near Infrared ◽  
Starch Content ◽  
Nir Spectroscopy ◽  
Water Soluble ◽  
Starch Accumulation ◽  
Soluble Carbohydrates ◽  
Spectroscopic Techniques ◽  
Absorption Bands ◽  
Physiological Processes ◽  
Water Soluble Carbohydrates

The role of bread, pasta and related products produced from milled wheat seeds is important to the human diet, so monitoring changes of starch content in developing grain is essential. Immature wheat grains are also used as a functional food, particularly as a source of water-soluble carbohydrates. The amount and variation in content of different carbohydrates changes considerably during maturation and these changes were non-destructively monitored in developing grain using near infrared (NIR) spectroscopy. Characteristic changes in three carbohydrate absorption bands [1585–1595 nm (Carbohydrate I), 2270–2280 nm (Carbohydrate II) and 2325–2335 nm (Carbohydrate III)] were identified and it was concluded that the different dynamics of carbohydrates (starch accumulation as well as synthesis/decomposition of water-soluble carbohydrates) could be followed sensitively by monitoring these three different regions of NIR spectra. Carbohydrate I represents the effect of starch accumulation during maturation based on the vibrations of intermolecular hydrogen bonded O–H groups in polysaccharides. Carbohydrate II is the manifestation of O–H stretching and C–C stretching vibrations existing unengaged in water-soluble carbohydrates while Carbohydrate III describes the changes in C–H stretching and deformation band of poly- and mono-oligosaccharides. NIR spectroscopic techniques are shown to be effective in monitoring plant physiological processes and the spectra have hidden information for predicting the stage of growth in wheat seed.

scholarly journals Effect of Abscisic Acid on Growth Correlation in Vitis Vinifera L.

1970 ◽  
Vol 23 (2) ◽  
pp. 479 ◽  
Author(s):  
MG Mullins ◽  
Daphne J Osborne
Keyword(s):  
Abscisic Acid ◽  
Vitis Vinifera ◽  
Adventitious Roots ◽  
Vitis Vinifera L ◽  
Bud Burst ◽  
Endogenous Cytokinin ◽  
Rooted Cuttings ◽  
Grape Vine ◽  
Cytokinin Synthesis ◽  
A Site

The inflorescences of woody grape vine cuttings usually atrophy if bud burst precedes emergence of adventitious roots (Mullins 1967). Roots are a site of cytokinin synthesis (Kende 1964; Itai and Vaadia 1965), and the ascending sap of vines is rich in endogenous cytokinin (Loeffler and Van Over beek 1964; Nitsch and Nitsch 1965; Skene and Kerridge 1967). The inflorescences of vine cuttings are retained and continue to grow if roots are initiated before bud burst, and the inflorescences of non-rooted cuttings are retained when treated with synthetic cytokinins (Mullins 1967).

scholarly journals Silencing GhAGPL1 Reduces the Quality and Quantity of Corms and Cormels in Gladiolus

2017 ◽  
Vol 142 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Shanshan Seng ◽  
Jian Wu ◽  
Jiahui Liang ◽  
Fengqin Zhang ◽  
Qiuyan Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Starch Content ◽  
Large Subunit ◽  
Starch Synthesis ◽  
Transcript Level ◽  
Starch Accumulation ◽  
Sucrose Content ◽  
Gladiolus Hybridus ◽  
Rate Limiting

Starch accumulation is important during com development. ADP-glucose pyrophosphorylase (AGPase) is the rate-limiting enzyme in starch synthesis. AGPL is the large subunit of AGPase. Here, we isolated and characterized the large subunit of AGPase gene GhAGPL1 in gladiolus (Gladiolus hybridus). GhAGPL1 was highly expressed in sink organs (cormels and corms). The expression of GhAGPL1 was induced by glucose, sucrose, and mannitol, and it was repressed by abscisic acid (ABA). Overexpression of GhAGPL1 in the arabidopsis (Arabidopsis thaliana) apl1 mutant resulted in complementation of AGPase activity and thus starch synthesis. Silencing GhAGPL1 in gladiolus decreased the transcript level of GhAGPL1 and GhSus, and resulted in the reduction of AGPase activity and starch content in gladiolus corm and cormel. Meanwhile, sucrose content was higher in GhAGPL1-silenced corm. Surprisingly, silencing GhAGPL1 in gladiolus produced smaller corms and fewer number of cormels. Overall, GhAGPL1 contributed to the quality and quantity of gladiolus corms and cormels.

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

scholarly journals Interplay among Antioxidant System, Hormone Profile and Carbohydrate Metabolism during Bud Dormancy Breaking in a High-Chill Peach Variety

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 560
Author(s):  
José A. Hernández ◽  
Pedro Díaz-Vivancos ◽  
José Ramón Acosta-Motos ◽  
Nuria Alburquerque ◽  
Domingo Martínez ◽  
...  
Keyword(s):  
Starch Content ◽  
Sugar Metabolism ◽  
Bud Dormancy ◽  
Dormancy Breaking ◽  
Prunus Species ◽  
Physiological Factors ◽  
Antioxidant Metabolism ◽  
Hormone Profile ◽  
Aba Content ◽  
Gas Ratio

(1) Background: Prunus species have the ability to suspend (induce dormancy) and restart growth, in an intricate process in which environmental and physiological factors interact. (2) Methods: In this work, we studied the evolution of sugars, antioxidant metabolism, and abscisic acid (ABA) and gibberellins (GAs) levels during bud dormancy evolution in a high-chill peach variety, grown for two seasons in two different geographical areas with different annual media temperature, a cold (CA) and a temperate area (TA). (3) Results: In both areas, starch content reached a peak at ecodormancy, and then decreased at dormancy release (DR). Sorbitol and sucrose declined at DR, mainly in the CA. In contrast, glucose and fructose levels progressively rose until DR. A decline in ascorbate peroxidase, dehydroascorbate reductase, superoxide dismutase and catalase activities occurred in both seasons at DR. Moreover, the H2O2-sensitive SOD isoenzymes, Fe-SOD and Cu,Zn-SOD, and two novel peroxidase isoenzymes, were detected. Overall, these results suggest the occurrence of a controlled oxidative stress during DR. GA7 was the major bioactive GA in both areas, the evolution of its levels being different between seasons and areas. In contrast, ABA content decreased during the dormancy period in both areas, resulting in a reduction in the ABA/total GAs ratio, being more evident in the CA. (4) Conclusion: A possible interaction sugars-hormones-ROS could take place in high-chill peach buds, favoring the DR process, suggesting that, in addition to sugar metabolism, redox interactions can govern bud DR, regardless of chilling requirements.

scholarly journals Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1084
Author(s):  
Ivan N. Ivanov ◽  
Vilém Zachleder ◽  
Milada Vítová ◽  
Maria J. Barbosa ◽  
Kateřina Bišová
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Starch Content ◽  
Light Availability ◽  
Fold Increase ◽  
Pilot Scale ◽  
Temperature Treatment ◽  
Control Culture ◽  
Starch Accumulation ◽  
Cellular Division ◽  
Starch Production

An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 °C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1–2 days, compared with 5 days for the control culture at the optimal temperature (30 °C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with.

scholarly journals Molecular mechanism underlying the effect of maleic hydrazide treatment on starch accumulation in S. polyrrhiza 7498 fronds

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yerong Zhu ◽  
Xiaoxue Li ◽  
Xuan Gao ◽  
Jiqi Sun ◽  
Xiaoyuan Ji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Carbon Fixation ◽  
Maleic Hydrazide ◽  
Starch Content ◽  
Vegetative Reproduction ◽  
Photosynthetic Pigment ◽  
Starch Accumulation ◽  
Chlorophyll Fluorescence Parameters ◽  
Significant Difference ◽  
Starch Production

Abstract Background Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. The starch content can be promoted by plant growth regulators after the vegetative reproduction being inhibited. Maleic hydrazide (MH) has been reported to inhibit plant growth, meantime to increase biomass and starch content in some plants. However, the molecular explanation on the mechanism of MH action is still unclear. Results To know the effect and action mode of MH on the growth and starch accumulation in Spirodela polyrrhiza 7498, the plants were treated with different concentrations of MH. Our results showed a substantial inhibition of the growth in both fronds and roots, and increase in starch contents of plants after MH treatment. And with 75 µg/mL MH treatment and on the 8th day of the experiment, starch content was the highest, about 40 mg/g fresh weight, which is about 20-fold higher than the control. The I2-KI staining and TEM results confirmed that 75 µg/mL MH-treated fronds possessed more starch and big starch granules than that of the control. No significant difference for both in the photosynthetic pigment content and the chlorophyll fluorescence parameters of PII was found. Differentially expressed transcripts were analyzed in S. polyrrhiza 7498 after 75 µg/mL MH treatment. The results showed that the expression of some genes related to auxin response reaction was down-regulated; while, expression of some genes involved in carbon fixation, C4 pathway of photosynthesis, starch biosynthesis and ABA signal transduction pathway was up-regulated. Conclusion The results provide novel insights into the underlying mechanisms of growth inhibition and starch accumulation by MH treatment, and provide a selective way for the improvement of starch production in duckweed.

Abscisic Acid Content and Osmotic Relations of Salt-Stressed Grapevine Leaves

1981 ◽  
Vol 8 (5) ◽  
pp. 443 ◽  
Author(s):  
WJS Downton ◽  
BR Loveys
Keyword(s):  
Abscisic Acid ◽  
Acid Concentration ◽  
Stomatal Resistance ◽  
Vitis Vinifera L ◽  
Phaseic Acid ◽  
Ion Content ◽  
Grapevine Leaves ◽  
Abscisic Acid Content ◽  
Osmotic Potentials ◽  
Reducing Sugar Concentration

Changes in abscisic acid, phaseic acid, stomatal resistance, water potential, osmotic potential, turgor potential, proline, reducing sugars and ion content (Na+, K+, Cl-) in leaves from grapevines (Vitis vinifera L.) subjected to 0, 25, 50 or 100 mM NaCl (osmotic potentials of 0, - 0.1, - 0.2 and - 0.4 MPa, respectively) were monitored over a 3-week period. Abscisic acid concentration increased within 6 h for the 50 and 100 mM NaCl-treated vines. Proline did not accumulate until the next day for the 100 mM NaCl-treated plants and continued to accumulate for the duration of the experiment. Phaseic acid showed kinetics consistent with its being derived from abscisic acid. Stomatal resistance to water vapour exchange increased in the salt-treated plants over the course of the experiment despite a decline in abscisic acid concentration after the initial upsurge. Reducing sugar concentration showed an early upsurge, its contribution to osmotic readjustment being at least equal to that of accumulated Na+, K+ and Cl- the day after stress began. Potassium was preferentially accumulated over sodium into leaves during the first 8 days of the experiment and the sum of these two cations generally balanced accumulating chloride. Except for an initial loss of turgor in vines given 100 mM NaCl, turgor potential was maintained within 0.1 MPa of control plants for all of the treatments throughout the experiment.

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