scholarly journals Similarity and Phylogenetic Analysis of Herbicide-Resistant Goosegrass (Eleusine indica) Biotypes

2019 ◽  
Vol 34 (2) ◽  
pp. 162
Author(s):  
Koko Tampubolon ◽  
Fransisca Natalia Sihombing ◽  
Elseria Siburian ◽  
Yustina Sri Sulastri ◽  
Zavandri Purba ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Evolutionary Genetics ◽  
Review Article ◽  
Homologous Sequence ◽  
Molecular Approach ◽  
Neighbor Joining ◽  
Eleusine Indica ◽  
Herbicide Resistant ◽  
Nucleotide Alignment ◽  
Selection Of

Molecular approach as a herbicide-resistant agent detector is an initial solution before controlling of <em>Eleusine indica </em>weed. This review article is aimed to obtain the basic data of similarity and phylogenetic values among herbicide-resistant <em>E. indica</em><em> </em>biotypes. This research used a descriptive analytical method. The analysis involved 14 nucleotide sequences of herbicide-resistant <em>E.indica </em>biotypes obtained from the National Center for Biotechnology Information. The nucleotide alignment of herbicide-resistant <em>E. indica </em>biotypes was conducted with ClustaLW using the Molecular Evolutionary Genetics Analysis (MEGA) v. 5.05 software based on a method of neighbor-joining tree construct/test. The results showed that two nucleotides of 7,921 herbicide-resistant <em>E. indica</em> biotypes were homologous (sequence 1,231 and sequence 1,408). The similarity values among herbicide-resistant <em>E. indica</em> biotypes ranged from 0.00 to 1.19. The information of phylogenetic pattern is needed in the selection of the herbicides mode of action rotation in order to control herbicide-resistant <em>E. indica </em>biotypes.

scholarly journals Advances in Fabricating the Electrospun Biopolymer-Based Biomaterials

2021 ◽  
Vol 12 (2) ◽  
pp. 26
Author(s):  
Sebastian Wilk ◽  
Aleksandra Benko
Keyword(s):  
Mechanical Properties ◽  
Whey Protein ◽  
Antimicrobial Properties ◽  
Protein Isolate ◽  
Review Article ◽  
Animal Tissues ◽  
Green Material ◽  
Good Potential ◽  
Fibrous Morphology ◽  
Selection Of

Biopolymers formed into a fibrous morphology through electrospinning are of increasing interest in the field of biomedicine due to their intrinsic biocompatibility and biodegradability and their ability to be biomimetic to various fibrous structures present in animal tissues. However, their mechanical properties are often unsatisfactory and their processing may be troublesome. Thus, extensive research interest is focused on improving these qualities. This review article presents the selection of the recent advances in techniques aimed to improve the electrospinnability of various biopolymers (polysaccharides, polynucleotides, peptides, and phospholipids). The electrospinning of single materials, and the variety of co-polymers, with and without additives, is covered. Additionally, various crosslinking strategies are presented. Examples of cytocompatibility, biocompatibility, and antimicrobial properties are analyzed. Special attention is given to whey protein isolate as an example of a novel, promising, green material with good potential in the field of biomedicine. This review ends with a brief summary and outlook for the biomedical applicability of electrospinnable biopolymers.

scholarly journals Molecular record for the first authentication of Isaria cicadae from Vietnam

2021 ◽  
Vol 16 (1) ◽  
pp. 711-718
Author(s):  
Thuan Duc Lao ◽  
Hanh Van Trinh ◽  
Loi Vuong ◽  
Luyen Tien Vu ◽  
Thuy Ai Huyen Le ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genomic Dna ◽  
Morphological Analysis ◽  
Phylogenetic Analyses ◽  
Neighbor Joining ◽  
Pcr Assay ◽  
Parsimony Method ◽  
Maximum Parsimony Method ◽  
Cordyceps Cicadae ◽  
High Bootstrap

Abstract The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae, belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including ITS, nrLSU, nrSSU, Rpb1, and Tef1 was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify ITS, nrLSU, nrSSU, Rpb1, and Tef1 gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus Verticillium. The phylogenetic analysis results indicated that T011 was accepted at subclade Cordyceps and significantly formed the monophyletic group with referent Cordyceps cicadae (Telemorph of Isaria cicadae) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the Isaria cicadae. In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as Isaria cicadae.

scholarly journals Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

2021 ◽  
pp. 1-11
Author(s):  
Andrea Highfield ◽  
Angela Ward ◽  
Richard Pipe ◽  
Declan C. Schroeder
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dunaliella Salina ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Hypersaline Environments ◽  
Rdna Its ◽  
Β Carotene ◽  
Selection Of

Abstract Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.

scholarly journals Herbicide-resistant crops in resistant weed management : An industrial perspective

2005 ◽  
Vol 75 (4) ◽  
pp. 79-84 ◽  
Author(s):  
D. Shaner
Keyword(s):  
Weed Control ◽  
Weed Management ◽  
Wild Species ◽  
Integrated Weed Management ◽  
Herbicide Resistant ◽  
Resistant Varieties ◽  
Selection Of

Some of the first products of biotechnology to reach the marketplace have been herbicide-resistant crops. Industry sees the development of herbicide-resistant varieties as a way to increase the availability of proven herbicides for a broader range of crops. However, the development of herbicide- resistant crops requires special attention to potential environmental questions such as herbicide usage, selection of resistant weed biotypes and spread of resistance from the resistant crop to wild species. Industry is actively addressing these concerns during the process of development. Proper development and use of herbicide-resistant crops in integrated weed management programs will provide farmers with increased flexibility, efficiency, and decreased cost in their weed control practices without increasing the risk of herbicide-resistant weeds. Furthermore, herbicide-resistant crops should prove to be valuable tools in managing herbicide- resistant weeds.

scholarly journals Phylogenetic analysis of spring viremia of carp virus (SVCV) identified in Poland

2019 ◽  
Vol 75 (01) ◽  
pp. 6159-2019
Author(s):  
JOANNA MAJ-PALUCH ◽  
EWA BORZYM ◽  
MAREK MATRAS ◽  
MAGDALENA STACHNIK ◽  
MICHAŁ REICHERT
Keyword(s):  
Phylogenetic Analysis ◽  
Preventive Measures ◽  
Genetic Material ◽  
Point Mutations ◽  
The United States ◽  
Nucleotide Sequence Analysis ◽  
Neighbor Joining ◽  
The Czech Republic ◽  
Virus Isolates ◽  
Family Rhabdoviridae

Spring viremia of carp (SVC) is a disease caused by a virus belonging to the genus Vesiculovirus, family Rhabdoviridae. The SVC virus is divided into four genogroups, Ia, Ib, Ic, and Id, due to its geographical distribution. This study aimed to identify the genotype of the SVC virus circulating in Poland. Polish SVC virus isolates were propagated on EPC and FHM cell lines, and genetic material (RNA) was isolated. The virus was detected in test samples by reverse transcription, sequenced and analyzed using MEGA 6.06 software. The phylogenetic tree was constructed by the Neighbor-Joining method. The results of phylogenetic analysis revealed the presence of two genogroups of the SVC virus in Poland. Most of Polish isolates belonged to the genogroup Id, as do isolates AY196200 from the Czech Republic, Z37505 from Belgium and EF593149 from the United States. Only two Polish isolates from Silesian Voivodeship were more closely related to Chinese and US isolates belonging to the genogroup Ia. There were no isolates belonging to the genogroups Ib and Ic. Nucleotide sequence analysis revealed certain point mutations between particular isolates. Knowledge on the genetic variants of the SVC virus circulating in Poland will be useful in epizootic investigations and preventive measures to protect Polish aquaculture from new variants from the neighboring countries

scholarly journals Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3746 ◽  
Author(s):  
Peter Shum ◽  
Lauren Moore ◽  
Christophe Pampoulie ◽  
Cristina Di Muri ◽  
Sara Vandamme ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Fisheries Management ◽  
North Atlantic ◽  
Molecular Approach ◽  
Mtdna Variation ◽  
Control Region Sequences ◽  
Market Product ◽  
Reference Sequences ◽  
Pacific Rockfish

Morphology-based identification of North AtlanticSebasteshas long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North AtlanticSebastescomprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicusandS. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification ofSebastesmarket samples.

scholarly journals ANTINATAL DIETARY CARE - A REVIEW ARTICLE

2017 ◽  
Vol 5 (8) ◽  
pp. 202-206
Author(s):  
Ankita Rajendra Jain ◽  
Vishala S. T.
Keyword(s):  
Pregnant Woman ◽  
Physical Health ◽  
Pregnant Women ◽  
Review Article ◽  
Mental And Physical Health ◽  
Selection Of

As per Ayurvedic literature each month of pregnancy has its unique requirement which is very important for every pregnant woman. There is detail description regarding pathya-apathya for garbhini. The pregnancy diet ideally should be light, nutritious, easily digestable and rich in proteins, minerals, and vitamins. Selection of right kind of pregnancy diet can decide the mental and physical health of child so it becomes important to pay extra attention for what u should eat and what u should not. Diet guidelines described under garbhiniparicharya is ideal protocol for pregnant women.

scholarly journals Genetic Transformation of Common Bean (Phaseolus vulgarisL.) with theGusColor Marker, theBarHerbicide Resistance, and the Barley (Hordeum vulgare)HVA1Drought Tolerance Genes

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Kingdom Kwapata ◽  
Thang Nguyen ◽  
Mariam Sticklen
Keyword(s):  
Hordeum Vulgare ◽  
Common Bean ◽  
Late Embryogenesis Abundant ◽  
Shoot Meristem ◽  
Late Embryogenesis Abundant Protein ◽  
Herbicide Resistant ◽  
Late Embryogenesis ◽  
Color Marker ◽  
Selection Of

Five common bean (Phaseolus vulgarisL.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes includedguscolor marker which visually confirmed transgenic events, thebarherbicide resistance selectable marker used forin vitroselection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of betterP. vulgarisgermplasm.

scholarly journals Detection, Quantitation, and Phylogenetic Analysis of Noroviruses in Japanese Oysters

2003 ◽  
Vol 69 (10) ◽  
pp. 5782-5786 ◽  
Author(s):  
Tomoko Nishida ◽  
Hirokazu Kimura ◽  
Mika Saitoh ◽  
Michiyo Shinohara ◽  
Masahiko Kato ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Virus Type ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
The Other ◽  
Capsid Gene ◽  
Neighbor Joining ◽  
Norwalk Virus ◽  
Reverse Transcription Pcr

ABSTRACT Noroviruses (NVs) cause many cases of oyster- or clam-associated gastroenteritis in various countries. We collected 191 samples from Japanese oysters intended for raw consumption that had been harvested from the sea in two different areas between December 2001 and February 2002. To detect, quantitate, and phylogenetically analyze the NV genome in purified concentrates from the stomachs and digestive diverticula of these oysters, we amplified the NV capsid gene by reverse transcription-PCR. Phylogenetic analysis was performed by using the neighbor-joining method. We detected the NV genome in 17 of 191 oysters (9%). Phylogenetic analysis indicated genogroup I (Norwalk virus type) in 3 of the 17 oysters and genogroup II (Snow Mountain virus type) in the other 14. Both genogroups showed wide genetic diversity. To quantify the NV capsid gene in these oysters, we performed real-time PCR using genogroup-specific probes. More than 102 copies of the NV genome were detected in 11 of 17 oysters. The results suggested that about 10% of Japanese oysters intended for raw consumption harbored NVs, and more than 50% of those oysters in which NVs were detected had a large amount.

REVIEW ARTICLE

PEDIATRICS ◽  
1956 ◽  
Vol 17 (6) ◽  
pp. 897-924
Author(s):  
Lytt I. Gardner
Keyword(s):  
Adrenal Tumor ◽  
Review Literature ◽  
Review Article ◽  
Adrenal Hyperplasia ◽  
The Past ◽  
Developing Area ◽  
Arbitrary Selection ◽  
Selection Of ◽  
Colorimetric Methods ◽  
Made In

THE IDENTIFICATION and measurement of adrenocortical steroids in body fluids over the past 2 decades have made possible an extensive exploration of adrenal function. This review is restricted to adrenocortical metabolism in the fetus, infant and child. No attempt will be made to cover all of this rapidly developing area of endeavor. Certain aspects have been treated in other review articles, to which the reader is referred. The reviews by Moore on fetal endorinology, Klein on neonatal adrenal physiology, Lieberman and Teich and Roberts and Szego on steroid biochemistry, White and Wettstein and Anner on adrenal cortical hormones, Ingle on cortisone and Gaunt, Renzi and Chart on aldosterone are especially useful. The books by Wilkins and by Talbot and colleagues contain helpful treatments of this area. Due to the prolific nature of even the review literature, it is necessary to make a rather arbitrary selection of material. NEWER METHODOLOGY Within recent years a number of methods have been developed for the estimation of hormones in urine and plasma. Several techniques for the chromatographic separation of the 17-ketosteroids in urine have been described. Of particular value in the diagnosis of virilizing adrenal tumor has been the development of colorimetric methods for the estimation of dehydroepiandrosterone in urine. Some progress has been made in the estimation of corticosteroids in the urine. Techniques for the measurement of 11-oxygenated neutral 17-ketosteroids, pregnanetriol and pregnanediol in the urine of patients with congenital adrenal hyperplasia have been described. There has been a blossoming of methods for estimating steroids in plasma. Several varieties of techniques are now available for the measurement of corticosteroids and 17-ketosteroids in plasma.

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