scholarly journals Effects of light intensity and co-inoculation of arbuscular mycorrhizal fungi and rhizobium on root growth and nodulation of Indigofera tinctoria

2020 ◽  
Vol 17 (2) ◽  
pp. 94
Author(s):  
Maria Theresia Sri Budiastuti ◽  
Djoko Purnomo ◽  
Supriyono Supriyono ◽  
Bambang Pujiasmanto ◽  
Desy Setyaningrum
Keyword(s):  
Light Intensity ◽  
Root Growth ◽  
Root Length ◽  
Mycorrhizal Fungi ◽  
Root Biomass ◽  
Block Design ◽  
Fresh Weight ◽  
Microbial Inoculation ◽  
Four Levels ◽  
Indigofera Tinctoria

<p class="Default"><em>Indigofera tinctoria</em> is a legume that is cultivated as a source of natural indigo dyes. As a legume, <em>Indigofera tinctoria</em> is capable of symbiosis with soil microbes. This study evaluates the effects of light intensity and microbial inoculation on root growth and nodulation. The study used a complete randomized block design with a split-plot pattern. Light intensity was the main plot with four levels of light intensity 100%, 50%, 25%, and 10%. Microbial inoculation was a subplot with four levels without inoculation, mycorrhizae inoculation, rhizobium inoculation, and double inoculation with both mycorrhizae and rhizobium. The results obtained show that light intensity and microbial inoculation affected root length, root fresh weight, root biomass, and the number of nodules. 50% light intensity was optimum for root length, while 100% light intensity was optimum for root fresh weight, root biomass, and a number of nodules. Root growth and nodulation were further increased with double inoculation. The combination of light intensity and microbial inoculation affected root biomass and nodulation. The combination of 100% light intensity and double inoculation resulted in the highest root biomass and nodule numbers. Mycorrhizae and rhizobium have a synergistic relationship to nodulation and root growth. Double inoculation with mycorrhizae and rhizobium efficiently increased root biomass and the number of nodules under low or high light intensity.</p>

scholarly journals Effects of warming and fertilization interacting with intraspecific competition on fine root traits of Picea asperata

2020 ◽  
Author(s):  
Dan-Dan Li ◽  
Hong-Wei Nan ◽  
Chun-Zhang Zhao ◽  
Chun-Ying Yin ◽  
Qing Liu
Keyword(s):  
Root Growth ◽  
Climate Warming ◽  
Tree Growth ◽  
Root Length ◽  
Root Biomass ◽  
Fine Root ◽  
Root Traits ◽  
Root Surface ◽  
Root Branching ◽  
Picea Asperata

Abstract Aims Competition, temperature, and nutrient are the most important determinants of tree growth in the cold climate on the eastern Tibetan Plateau. Although many studies have reported their individual effects on tree growth, little is known about how the interactions of competition with fertilization and temperature affect root growth. We aim to test whether climate warming and fertilization promote competition and to explore the functional strategies of Picea asperata in response to the interactions of these factors. Methods We conducted a paired experiment including competition and non-competition treatments under elevated temperature (ET) and fertilization. We measured root traits, including the root tip number over the root surface (RTRS), the root branching events over the root surface (RBRS), the specific root length (SRL), the specific root area (SRA), the total fine root length and area (RL and RA), the root tips (RT) and root branching events (RB). These root traits are considered to be indicators of plant resource uptake capacity and root growth. The root biomass and the nutrient concentrations in the roots were also determined. Important Findings The results indicated that ET, fertilization and competition individually enhanced the nitrogen (N) and potassium (K) concentrations in fine roots, but they did not affect fine root biomass or root traits, including RL, RT, RA and RB. However, both temperature and fertilization, as well as their interaction, interacting with competition increased RL, RA, RT, RB, and nutrient uptake. In addition, the SRL, SRA, RTRS and RBRS decreased under fertilization, the interaction between temperature and competition decreased SRL and SRA, while the other parameters were not affected by temperature or competition. These results indicate that Picea asperata maintains a conservative nutrient strategy in response to competition, climate warming, fertilization, and their interactions. Our results improve our understanding of the physiological and ecological adaptability of trees to global change.

scholarly journals Penggunaan Limbah Baglog Tiram dan Jenis Nutrisi Terhadap Pakcoy Pada Hidroponik Substrat

2017 ◽  
Vol 19 (1) ◽  
pp. 28
Author(s):  
Retno Bandriyati Arni Putri ◽  
Trijono Djoko Sulistyo ◽  
Chairul Anwar
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Root Biomass ◽  
Oyster Mushroom ◽  
Leaf Biomass ◽  
Fresh Weight ◽  
Root Volume ◽  
Random Design

<p>The increase of oyster mushroom farmers in wonogiri will be impact immediately on increasing waste baglog. One of the utilization is to be medium on hydroponic substrate for growing mustard greens and it will combined with charcoal and various types of nutrients. The research was conducted from October to December 2015 at the Greenhouse C and Laboratory EMPT UNS.the research employed completely random design (CRD) with two factor that is ratio of medium and various types of nutrients. The result of research showed that interaction between ratio of medium and various types affected significantly  the parameters of leaf area, leaf fresh weight and leaf biomass. Root fresh weight, root volume, root length and root biomass is only influenced by the type of nutrition with the ab mix is the most good in improving and supplying nutrients</p>

scholarly journals RESPONS FISIOLOGI, PERTUMBUHAN, PRODUKSI DAN SERAPAN P BAWANG MERAH (Allium ascalonicum L.) TERHADAP PEMBERIAN TRICHOKOMPOS TANDAN KOSONG KELAPA SAWIT (TKKS) TERFORMULASI DAN PUPUK P DI LAHAN GAMBUT

2016 ◽  
Vol 6 (2) ◽  
pp. 15 ◽  
Author(s):  
ZALDI ARMAN ◽  
NELVIA NELVIA ◽  
ARMAINI ARMAINI
Keyword(s):  
Plant Height ◽  
Transpiration Rate ◽  
Block Design ◽  
Physiological Responses ◽  
P Uptake ◽  
Growth Responses ◽  
Fresh Weight ◽  
Four Levels ◽  
Production Response ◽  
P Fertilizers

The study aims to determine the effect of formulated PEFB trichokompos and P fertilizers  on growth, physiology, production and P uptake of onion in peatlands. This research used experimental factorial randomized completely block design and three replications. Trichokompos PEFB formulated as the first factor is composed of four levels (0, 5, 10 and 15 ton ha-1) and P fertilizers as the second factor consists of four levels (0, 120, 180 and 240 kg ha-1 P₂O5). Parameters measured were chlorophyll content, photosynthesis rate, stomataH2Oconductivity , the concentration of CO2 in the cell, transpiration rate, plant height, number of tillers, number of tubers per hill, the diameter of the bulbs, fresh weight of tuber per hill, plant dry weight and P uptake. The results showed trichokompos PEFB formulated 15 ton ha-1 can increase the response of onion production as tuber diameter and fresh weight per hill and P uptake response respectively by 25.58%, 74.92% and 76.39%. While the physiological responses seen to decrease thestomatal H2O conductivity and transpiration rate of respectively 21.43% and 28.79% compared with no PEFB formulatedtrichokompos. Provision of fertilizer P 120 P2O5 ha-1 can improve physiological responses such as H2O on stomatal conductivity, increased growth responses such as plant height, such as the production response tuber diameter and fresh weight per hill and P uptake response respectively by 7.69%, 22.25%, 26.23%, 56.21% and 44.26% compared with no fertilizer P. combination trichokompos PEFB formulated 15 ton ha-1 with manure P 120 P2O5 ha-1 is the best treatment combination, with the highest production response namely 7.65 g (1.91 ton ha-1) fresh weight per hill and increased 214.81% compared with no treatment trichokompos PEFB formulated and fertilizer P.

scholarly journals Pengaruh Aplikasi Biostimulant terhadap Pertumbuhan dan Produksi Selada (Lactuca sativa L.)

2020 ◽  
Vol 16 (2) ◽  
pp. 109-117
Author(s):  
Primarine R Tahapary ◽  
Herman Rehatta ◽  
Henry Kesaulya
Keyword(s):  
Vitamin E ◽  
Plant Growth ◽  
Vitamin C ◽  
Leaf Area ◽  
Root Length ◽  
Block Design ◽  
Growth And Yield ◽  
Leaf Number ◽  
Root Number ◽  
Fresh Weight

Lettuce is a type of vegetable that is very popular with the people of Indonesia. It contains healthy nutrients including minerals, vitamins, antioxidants, potassium, iron, folate, carotene, vitamin C and vitamin E. Biostimulants has the ability to stimulate new root formation, improve plant root systems, increase the number of tillers, and increase yield. Biostimulant concentration and site of administration play a role in providing nutrients for the needs of lettuce plants. To obtain the right concentration and place of administration for the growth and yield of lettuce, the experiment was carried out using a 2-factor Randomized Block Design. The first factor was the concentration of biostimulants (K) with 6 levels of concentration including: K0 (without giving biostimulants), K1 (giving biostimulants at a concentration of 1 mL/L of water), K2 (giving of biostimulants at 2 mL/L of water), K3 (giving biostimulants at 3 mL/L of water). K4 (giving biostimulant at 4 mL/L water), K5 (giving biostimulant at 5 mL/L water). The second factor was the site of administration (T), namely: T1 (through the leaves) and T2 (through the soil). The interaction between biostimulant concentration and administration gave a very significant effect on leaf number, crop fresh weight, root length and root number. Biostimulant concentration (K) gave the percentage of plant growth in the variables of plant height (34.29%) and leaf area (47.34%). Place of administration (T) gave percentage of plant growth the variables of leaf area (21.08%) and root wet weight (0.52%). The interaction of biostimulant concentration and administration place (K x T) gave the percentages of plant growth variables of leaf number (15.5%), plant fresh weight (52.33%), root dry weight (2.30%), root length (16.53%), and root number (108.5%). The best treatment in this study was the provision of biostimulant at a concentration of 2 mL/L of water that was given through leaves. Keywords: administration place, biostimulant, concentration, growth and production, lettuce   ABSTRAK Selada merupakan jenis sayur yang sangat digemari oleh masyarakat Indonesia serta mengandung nutrisi yang sehat, yang meliputi mineral, vitamin, antioksidan, potassium, zat besi, folat, karoten, vitamin C dan vitamin E. Biostimulan mampu merangsang pembentukan akar baru, memperbaiki sistem perakaran tanaman, memperbanyak jumlah anakan, dan meningkatkan produksi tanaman. Konsentrasi biostimulan dan tempat pemberian merupakan interaksi yang berperan dalam menyediakan unsur hara bagi kebutuhan tanaman selada. Untuk memproleh konsentrasi dan tempat pemberian yang tepat bagi pertumbuhan dan produksi tanaman selada, dilakukan percobaan dengan menggunakan Rancangan Acak Kelompok 2 faktor. Faktor pertama adalah pemberian biostimulan (K) dengan 6 taraf konsentrasi meliputi: K0 (tanpa pemberian biostimulan), K1 (pemberian biostimulan dengan konsentrasi 1 mL/L air), K2 (pemberian biostimulan 2 mL/L air), K3 (pemberian biostimulan 3 mL/L air), K4 (pemberian biostimulan 4 mL/L air), K5 (pemberian biostimulan 5 mL/L air). Faktor kedua adalah tempat pemberian (T), yaitu: T1 (melalui daun) dan T2 (melalui tanah). Interaksi konsentrasi biostimulan berpengaruh sangat nyata terhadap variabel jumlah daun (helai), bobot segar tanaman (g), panjang akar (cm) dan jumlah akar (helai). Pada konsentrasi biostimulan (K) memberikan persentasepertumbuhan tanaman pada variabel tinggi tanaman (34,29%) dan luas daun (47,34%), Tempat pemberian (T) memberikan persentasepertumbuhan tanaman pada variabel luas daun (21,08%), bobot basah akar (0,52%), serta interaksi konsentrasi biostimulan dan tempat pemberian (KT) memberikan persentasepertumbuhan tanaman pada variabel jumlah daun (15,5%), bobot segar tanaman (52,33%), bobot kering akar (2,30%), panjang akar (16,53%), serta jumlah akar (108,5%). Perlakuan terbaik dalam penelitian ini adalah pemberian biostimulan konsentrasi 2 mL/L air dan diberikan melalui daun. Kata Kunci: biostimulan, konsentrasi, pertumbuhan dan produksi, selada, tempat pemberian,

scholarly journals Optimizing Phosphorus Application Rate and the Mixed Inoculation of Arbuscular Mycorrhizal Fungi and Phosphate-solubilizing Bacteria Can Improve the Phosphatase Activity and Organic Acid Content in Alfalfa Soil

2021 ◽  
Author(s):  
An Xiaoxia ◽  
Junying Liu ◽  
Xuanshuai Liu ◽  
Chunhui Ma ◽  
Qianbing Zhang
Keyword(s):  
Organic Matter ◽  
Phosphatase Activity ◽  
Mycorrhizal Fungi ◽  
Rhizosphere Soil ◽  
Block Design ◽  
High Yield ◽  
Phosphate Solubilizing Bacteria ◽  
High Quality ◽  
Four Levels ◽  
Phosphorus Application

Abstract Alfalfa (Medicago sativa L.) is an important high-quality legume forage, and phosphorus is an important nutrient element for high-quality and high-yield of alfalfa. This study assessed the effects bacteria and phosphorus (P) use efficiency of alfalfa soil under different P applications. In this experiment, a two-factor complete randomized block design was used. Four bacterial treatments were as follows: Funneliformis mosseae (Fm), Bacillus megaterium (Bm), double inoculation (Fm × Bm) and no inoculation bacteria (CK). There are four levels of phosphorus treatment, namely: phosphorus application 0 (P0), 50 (P1), 100 (P2), 150 mgžkg-1 (P3). There were 16 treatments in total, and each treatment was repeated 6 times. The results showed that the effects of single inoculation and mixed inoculation were significantly higher than those of noninoculation (P < 0.05). With the increase in phosphorus application, each index increased first and then decreased. The alkaline phosphatase activity (AKP) and organic matter (SOM) content in soi lincreased with the increase of cutting times, and the content of organic matter in rhizosphere soil was higher than that in non-rhizosphere soil. Principal component analysis (PCA) shows that the top three treatments were J3P2 > J3P1 > J3P3. Therefore, when (P2O5) was 100 mgžkg-1, the mixed inoculation of Fm × Bm could improve the phosphatase activity in alfalfa soil, promote the secretion of organic acids in rhizosphere soil and then improve the content of soil fertility.

scholarly journals Growth Assessment of Tropical Sugarbeet as Influenced by Spacing

2021 ◽  
Vol 23 (2) ◽  
pp. 51-58
Author(s):  
MAT Sohel ◽  
MAE Hossain ◽  
HP Roy ◽  
SM Reza ◽  
FH Shanta ◽  
...  
Keyword(s):  
Root Length ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Research Field ◽  
Shoot Length ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Number Of Leaves ◽  
Maximum Root

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58

scholarly journals RAINFALL REGIME ON FINE ROOT GROWTH IN A SEASONALLY DRY TROPICAL FOREST

2020 ◽  
Vol 33 (2) ◽  
pp. 458-469
Author(s):  
EUNICE MAIA DE ANDRADE ◽  
GILBERTO QUEVEDO ROSA ◽  
ALDENIA MENDES MASCENA DE ALMEIDA ◽  
ANTONIO GIVANILSON RODRIGUES DA SILVA ◽  
MARIA GINA TORRES SENA
Keyword(s):  
Root Growth ◽  
Root Length ◽  
Fine Roots ◽  
Root Biomass ◽  
Fine Root ◽  
Fine Root Biomass ◽  
Rainfall Regime ◽  
Seasonally Dry ◽  
Fine Root Length ◽  
Fine Root Growth

ABSTRACT Seasonally dry tropical forests (SDTF) usually present dry seasons of eight or more months. Considering the concerns about the resilience of SDTF to climate changes, the objective of this study was to evaluate the effect of the rainfall regime on fine root growth in a SDTF. The experiment started at the end of the wet season (July 2015), when fine roots were evaluated and ingrowth cores were implemented. The temporal growth of fine roots in the 0-30 cm soil layer was monitored, considering the 0-10, 10-20, and 20-30 cm sublayers, through six samplings from November 2015 to July 2017. The characteristics evaluated were fine root biomass, fine root length, fine root specific length, and fine root mean diameter. The significances of the root growths over time and space were tested by the Kruskal-Wallis test (p<0.05). Fine roots (Ø<2 mm) were separated and dried in an oven (65 °C) until constant weight. The root length was determined using the Giaroots software. The fine root biomass in July 2015 was 7.7±5.0 Mg ha-1 and the length was 5.0±3.2 km m-2. Fine root growth in SDTF is strongly limited by dry periods, occurring decreases in biomass and length of fine roots in all layers evaluated. Fine root growth occurs predominantly in rainy seasons, with fast response of the root system to rainfall events, mainly in root length.

scholarly journals Effect of Different Rates of N and Intrarow Spacing on Growth Performance of Lettuce (Lactuca sativa L.) in Gurage Zone, Wolkite University, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Bewuket Gashaw ◽  
Shewaye Haile
Keyword(s):  
Block Design ◽  
Maximum Yield ◽  
Dry Weight ◽  
Optimum Level ◽  
Plant Spacing ◽  
Fresh Weight ◽  
Randomized Complete Block Design ◽  
Main Effect ◽  
Demonstration Site ◽  
Four Levels

A field experiment was conducted in Wolkite University, Horticulture department practical demonstration site, from February to June 2019 with the objective of determining the optimum level of N and intrarow spacing for better growth of lettuce. The experiment consisted of four levels of N (N0 (0 kg/ha), N1 (50 kg/ha), N2 (100 kg/ha), and N3 (150 kg/ha)) and three levels of intrarow spacing (S1 (15 × 30 cm), S2 (20 × 30 cm), and S3 (25 × 30 cm)). The interrow spacing was maintained as 30 cm. The experiment was laid out in 3 × 4 factorial arrangement in a randomized complete block design (RCBD) and replicated three times. The main effect of nitrogen resulted maximum yield (9.45 ton/ha), dry weight per plant (28.75 g), and fresh weight per plant (57.57 g) which were recorded by the application of 150 kg/ha nitrogen. Likewise, the main effect of intrarow spacing gave maximum yield (8.01 ton/ha), dry weight per plant (21.31 g), and fresh weight per plant (59.15 g) from 25 × 30 cm plant spacing. For interaction effect, the highest yield (10.38 ton/ha), dry weight per plant (50.96 g), and fresh weight per plant (77.88 g) was found from N3S3 (150 kg/ha N and 25 × 30 cm plant spacing). Therefore, in the study, area using 150 kg/ha urea with 25 × 30 cm plant spacing could be advisable for optimum lettuce production.

Performance and gene effects for root characters and micronutrient uptake in wheat inoculated with arbuscular mycorrhizal fungi and Azotobacter chroococcum

2007 ◽  
Vol 55 (3) ◽  
pp. 325-330 ◽  
Author(s):  
R. Singh ◽  
R. Behl ◽  
P. Jain ◽  
N. Narula ◽  
K. Singh
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Root Length ◽  
Mycorrhizal Fungi ◽  
Root Biomass ◽  
Root Length Density ◽  
Arbuscular Mycorrhizal ◽  
Azotobacter Chroococcum ◽  
Gene Effects ◽  
Low Input ◽  
Micronutrient Uptake

The present investigation was conducted to investigate the impact of bio-inoculants on the magnitude and direction of gene effects and mean performance for root length density, root biomass per plant, AMF colonization in roots and micronutrient uptake (Cu, Fe, Mn, Zn) in wheat under low input field conditions. The material for study comprised three wheat cultivars, WH 147 (low mineral input), WH 533 (drought-tolerant), Raj 3077 (high mineral input) and six generations (P 1 , P 2 , F 1 , F 2 , BC 1 and BC 2 ) of three crosses, namely WH 147 × WH 533, WH 533 × Raj 3077 and WH 147 × Raj 3077. The experiment was conducted in a randomized block design with three replications having three treatments, i.e. (i) control; (ii) inoculation with arbuscular mycorrhizal fungi (AMF, Glomus fasciculatum ); (iii) dual inoculation with AMF and Azotobacter chroococcum ( Azc ). The fertilizer doses in all three treatments were 80 kg N + 40 kg P + 18 kg ZnSO 4 ha −1 . Root length density, root biomass per plant, AMF colonization in roots and Zn and Mn content were found to be maximum after dual inoculation with AMF+ Azc in all three crosses. Joint scaling tests revealed that additive-dominance gene effects were mainly operative in governing the expression of root biomass, Cu and Zn content in all three crosses for all three treatments (i.e. control, AMF and AMF + Azc ). Pedigree selection in crosses WH 147 × WH 533 and WH 147 × Raj 3077 could be effective for breeding pure lines of wheat for sustainable agriculture (low input genotypes responsive to biofertilizers such as AMF and Azotobacter ).

scholarly journals Growth, Yield and Profitability of Cabbage (Brassica oleracea L.) as Influenced by Applied Nitrogen and Plant Spacing

2016 ◽  
Vol 13 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Firoj Asadul Haque ◽  
N Islam ◽  
Md Nazrul Islam ◽  
Ahsan Ullah ◽  
Md Dulal Sarkar
Keyword(s):  
Brassica Oleracea ◽  
Block Design ◽  
Growth Yield ◽  
Plant Spacing ◽  
Fresh Weight ◽  
Randomized Complete Block Design ◽  
Four Levels ◽  
Applied Nitrogen

An experiment was conducted with cabbage during October 2012 to February 2013 at the Horticulture Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Four levels of nitrogen: viz. 0, 150, 250 and 350 kg ha-1 and three plant spacings: 50 x 30, 50 x 40 and 50 x 50 cm were applied in a Randomized Complete Block Design with three replications. Nitrogen @ 250 kg ha-1 with the spacing of 50 x 50 cm was more effective and produced the highest fresh weight of head(2.17 kg), marketable head yield (86.93 t ha-1). This treatment was also more profitable than the rest of the treatments, while the lowest profit was in N0S1.The Agriculturists 2015; 13(1) 35-45

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