scholarly journals Efficacy of Aloe vera Gel on the Excision Wound Healing in Sprague dawley Rats

2021 ◽  
Vol 4 (1) ◽  
pp. 46-49
Author(s):  
Bagus Uda Palgunadi ◽  
Asih Rahayu ◽  
Yos Adi Prakoso
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Sprague Dawley ◽  
Excision Wound ◽  
Promote Wound Healing ◽  
Living Things ◽  
Aloe Vera Gel ◽  
Sprague Dawley Rats ◽  
Better Than

Wound is a pathological processes in all living things. Wound includes the overlapping mechanisms. The failure in wound healing causes infection. This study aimed to analyze the efficacy of Aloe vera gel in excisionwound on the rat’s back. This study used 18 male, Sprague Dawley rats, and they were induced excision wound on the back. The rats were separated into 3 groups, K1 = control, K2 = treated with betadin gel, K3 = treated with Aloe vera gel. The therapy was given twice a day for 7 days. The wound measurement was observed in day 3 and 7. The data were analyzed using SPSS. The result showed that Aloe vera gel potentially promote wound healing through the decrease microscopical condition of wound, re-epithelialization, and deposition of collagen better than in group K1 and K2 (p≤0.05). In conclusion, Aloe vera gel promote excision wound healing in Sprague Dawley rats.

scholarly journals Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ahsanu Taqwim Hidayat ◽  
Muhamad Thohar Arifin ◽  
Muhammad Nur ◽  
Muflihatul Muniroh ◽  
Neni Susilaningsih
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Control Groups ◽  
Treatment Groups ◽  
A Value ◽  
Healing Response ◽  
Sprague Dawley Rats ◽  
Significant Difference

Objective. This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Methods. This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson’s trichrome staining and microscopic evaluation. Results. There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of p = 0.001 p < 0.05 , resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann–Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of p = 0.009 p < 0.05 . Conclusions. Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.

Oral administration of Aloe vera ameliorates wound healing through improved angiogenesis and chemotaxis in Sprague Dawley rats

2020 ◽  
Vol 21 ◽  
Author(s):  
Fatima Ali ◽  
Nadia Wajid ◽  
Maryam Ghulam Sarwar ◽  
Aamer Mahmood Qazi
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Growth Factor ◽  
Oral Administration ◽  
Aloe Vera ◽  
Collagen Content ◽  
Pcr Analysis ◽  
Sprague Dawley ◽  
Rt Pcr ◽  
Sprague Dawley Rats

Background:: Aloe vera has been reported as a topical antibiotic and healing agent for wounds but advantages of oral administration and mechanisms of wound healing have not been reported. Present study focuses on evaluation of effects of oral administration of Aloe vera for excisional cutaneous wounds in Sprague Dawley rats. Methods:: Sprague Dawley (SD) rats were inflicted with excisional wounds and then, either treated with Aloe vera orally (Aloe vera) or kept untreated (wound) while healthy rats were kept as control group. Wound area was measured from seven day to 21st day. Collagen content was estimated by hydroxyproline assay. Histology was analysed by hematoxylin and eosin staining. Angiogenesis was observed by indirect ELISA for Insulin like Growth Factor (IGF-1) and Vascular Endothelial Growth Factor (VEGF) protein from skin, serum and bone marrow. Chemotaxis was evaluated by RT-PCR analysis for Stromal cell-derived factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) from skin and bone marrow. Results:: Aloe vera healed wounds earlier than untreated rats with gradual improvement in wound areas and collagen content. Aloe vera also improved the expression of IGF-1 and VEGF in skin and bone marrow indicating improvement in angiogenesis. RT- PCR analysis showed increased expression of genes for chemotaxis (SDF-1 and CXCR-4), in skin and bone marrow.

scholarly journals N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

2021 ◽  
Vol 22 (14) ◽  
pp. 7549
Author(s):  
Wiktor Paskal ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
Albert Stachura ◽  
Kacper Pełka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Time Points ◽  
Model Study ◽  
Sprague Dawley Rats ◽  
Animal Model Study ◽  
Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

scholarly journals Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

2021 ◽  
Vol 33 (2) ◽  
pp. 145
Author(s):  
Hendry Rusdy ◽  
Astri Suryani Pasaribu Saruksuk ◽  
Rahmi Syaflida Dalimunte ◽  
Gostry Aldica Dohude
Keyword(s):  
Wound Healing ◽  
Tranexamic Acid ◽  
Tooth Extraction ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Jatropha Multifida ◽  
Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine 

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