scholarly journals Growth Response of Calopogonium caeruleum and Centrosema pubescens Ground Cover Crops toward Inoculation of Bradyrhizobium, Aeromonas punctata, and Acaulospora tuberculata

2016 ◽  
Vol 13 (1) ◽  
pp. 43
Author(s):  
Happy Widiastuti ◽  
NFN Suharyanto

<p>Planting of leguminous cover crops is a standard practise in preparing land for oil palm and rubber plantations. The synergism capability of Bradyrhizobium, Aeromonas punctata (phosphate solubilizing bacteria) and Acaulospora tuberculata (AM fungi) to increase growth of leguminous cover crops (Calopogonium caeruleum and Centrosema pubescens) was studied in a glass house experiment using polybag (10 x 10 cm) containing acid soil with low level nutrition of Ciomas, Bogor. Research results showed that Bradyrhizobium, A. punctata and A. tuberculata inoculation on C. caeruleum signifycantly enhanced plant height, and number of leaves. However, the treatment did not increase biomass and N, P, and K uptake of plant. Number of nodule were increase when the plant was inoculated with A. tuberculata alone or in combination with Bradyrhizobium and A. punctata. Centrosema pubescens gave good response when inoculated with A. tuberculata. However, dual inoculation of the two bacteria Bradyrhizobium and A. punctata with A. tuberculata signifycantly enhance plant height, plant biomass, N, P, and K plant uptake.</p><p> </p><p><strong>Abstrak</strong></p><p>Penanaman tanaman kacang-kacangan penutup tanah merupakan standar dalam penyiapan lahan pada pengusahaan kelapa sawit dan karet. Kemampuan sinergisme Bradyrhizobium (bakteri penambat N2), Aeromonas punctata (bakteri pelarut fosfat), dan Acaulospora tuberculata (cendawan mikoriza arbuskula) untuk meningkatkan pertumbuhan tanaman kacangkacangan penutup tanah (Calopogonium caeruleum dan Centrosema pubescens) dipelajari dalam percobaan rumah kaca menggunakan polibag berukuran 10 x 10 cm berisi tanah Ciomas Bogor yang bereaksi masam dan miskin hara. Hasil penelitian menunjukkan bahwa inokulasi Bradyrhizobium, A. Punctata, dan A. tuberculata pada C. caeruleum nyata meningkatkan pertumbuhan tinggi tanaman dan jumlah daun. Namun, perlakuan ini tidak meningkatkan biomasa dan serapan N, P, dan K tanaman. Jumlah bintil akar meningkat pada tanaman yang diinokulasi A. tuberculata sendiri atau dalam kombinasinya dengan Bradyrhizobium dan A. punctata. Centrosema pubescens menunjukkan respon yang baik bila diinokulasi dengan A. tuberculata. Bagaimanapun juga inokulasi dua bakteri, yaitu Bradyrhizobium and A. punctata yang disertai A. tuberculata nyata meningkatkan tinggi tanaman, biomasa, serapan N, P, dan K tanaman.</p>

2020 ◽  
Vol 5 (1) ◽  
pp. 300-304
Author(s):  
Betty Natalie Fitriatin ◽  
Dita Fauziah ◽  
Fabira Nur Fitriani ◽  
Dewi Nurma Ningtyas ◽  
Pujawati Suryatmana ◽  
...  

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.


2019 ◽  
Vol 20 (1) ◽  
pp. 19
Author(s):  
Ni Nyoman Ratini ◽  
I Wayan Supardi ◽  
Yuli Nurfadhillah

A research on the effect of photosynthetic active radiation (PAR) on the growth of green mustard plants has been conducted. The radiation source used is sunlight. Samples have been grouped as a sample which treated by red filter (P1), by orange filter (P2), by purple filter (P3), by green filter (P4), by blue filter (P5) and a sample without filter as a control (P0). Each sample consisted of four plants. The planting was carried out using polybags with compost media. Observations were made from the nursery phase to the slow vegetative phase (day 3rd, when all plants had grown shoots until day 63rd of the harvest). Parameters measured include light intensity, plant height and number of leaves. Measurement is done every three days. Also it measured plant biomass on the last day of observation (63rd day). The results showed that the intensity of each sample had an impact on the harvest. The best growth rate is obtained in P2, both in the nursery phase and fast vegetative phase i.e. 0.119 cm/day and 0.194 cm/day, respectively. While the highest growth rate was obtained in the P3 sample, namely the slow vegetative phase (0.035 cm/day). Overall the best planting results were obtained in P2 samples with plant height of 23.18 cm, number of leaves of 12 strands and plant biomass of 33.56 g.


2021 ◽  
Vol 9 (5) ◽  
pp. 145-156
Author(s):  
Collins EGBUCHUA ◽  
Emmanuel Chukudinife ENUJEKE

A field potted experiment was carried out in Asaba area of Delta State, Nigeria to evaluate sources and rates of organic manures (poultry and farmyard) on growth responses of Gongronema latifolium in an acid soil environment. It was a factorial experiment laid out in a randomized complete block design replicated 3 times. Data on plant height, number of leaves/branches, leaf area and stem girth were collected at 3, 5, 7, 9, 11 and 13 Weeks after sowing (WAS) and subjected to analysis of variance. The results that the effects on growth parameters of the crop were not significant and that higher application rates significantly increased plant height, number of leaves/branches and stem girth of G. latifolium. Application rates of 15 and 20 t/ha of both poultry and farm yard manures increased plant height from 10.45 cm to 12.52 cm and 8.12 cm and 10.68 cm respectively. The overall result indicated that the higher rate of organic manure, the better the response of growth indices of G. latifolium, though interaction effect was not significant. Poultry manure produced higher values of growth indices than farm yards manure, hence was recommended for increased productivity of G.latifolium in the study area.


2021 ◽  
Vol 15 ◽  
Author(s):  
Juvenaldo Florentino Canjá ◽  
Josimar De Azevedo ◽  
Geocleber Gomes de Sousa ◽  
Clarissa Lima Magalhães ◽  
Thales Vinícius De Araújo Viana

Zucchini culture is slightly sensitive to salinity and is among the ten vegetables of highest economic value, with characteristics of precocity and easy cultivation. These characteristics are some of the reasons for the expansion of its cultivation among small producers. Thus, the objective was to evaluate the effect of different levels of salinity in the irrigation water and biofertilizer types on the initial growth of the zucchini (Cucurbita pepo L.) culture. The experiment was carried out in the full sunlight in a randomized block design, in a 5x2 factorial arrangement. The treatments consisted of a combination of five types of biofertilizers (quail, sheep, mixed, bovine, and crab) and two salinity levels of the irrigation water (0.8 and 2.5 dS m-1), with five replicates. At 30 days after transplanting, the following variables were evaluated: electrical conductivity of the substrate, plant height, number of leaves, roots length, stem diameter, leaf area, chlorophyll content, dry mass of the aerial part, root dry mass, and total dry mass. Salt stress negatively interferes with the accumulation of zucchini plant biomass. Quail, bovine, and sheep biofertilizers are more efficient about plant height, number of leaves, and stem diameter. The sheep biofertilizer attenuates the salt stress for the dry mass of the aerial part, the root, and the total dry mass.


2020 ◽  
Vol 4 (2) ◽  
pp. 59
Author(s):  
Pauliz Budi Hastuti ◽  
Sri Manu Rohmiyati

<p>This research aimed to evaluate the effect of empty fruit bunches (efb) compost, phosphate-solubilizing bacteria (PSB), lime, and types of P fertilizer on the growth and availability of Phosphorus and P uptake in the pre-nursery (PN) oil palm seedlings in latosols. The pot experiments were arranged in a Factorial Completely Randomized method. The first factor was the application of efb compost: without efb compost, efb compost, without efb compost + PSB, and without efb compost + lime. The second factor was the types of P fertilizer: SP-36, RP, guano, and NPKMg + Urea as a control. The results showed that the combination of without efb compost +lime and RP fertilizer produces the highest number of leaves. The influence of a single factor showed that the application of without efb, efb compost, without efb compost +PSB, without efb compost + lime, resulted in the same growth of seedlings, except on plant height and the length of the leaf. The various types of P fertilizer led to the same growth of seedlings except for stem diameter. The highest available P was obtained in the combination of without efb compost + PSB with SP-36 fertilizer, which was 631.1% compared to control (without efb compost and NPKMg, Urea) and the lowest in the combination of without efb compost + lime with (NPKMg, Urea). The treatment without efb compost + lime with SP-36 fertilizer increased P uptake of leaves by 55.6% and stem by 47.1% compared to control.</p>


Jurnal Agro ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 40-54
Author(s):  
Andre Sparta ◽  
Arifah Rahmi ◽  
Panca Jarot Santoso ◽  
Ida Fitrianingsih

In the nursery, media composition plays an essential role in the growth and development of plant seedlings. Extract of Mexican sunflower (Tithonia diversifolia) could be suitable for replacing organic manure and rice husk charcoal during the seedling phase. The purpose of this study is to examine the ability of Mexican sunflower extract to replace organic manure and rice husk charcoal as the source of nutrients for banana seedling. The study was arranged in a Completely Randomized Design with seven treatments and three replications. Extract of Mexican sunflower promotes plant growth (plant height, number of leaves, and leaf area), improves plant biomass, and improves soil chemical properties (pH (H2O), C organic, and total N). Application twice a week of Mexican sunflower into soil medium resulted superior in plant height, the number of leaves, leaf area, and total biomass of banana seedlings compared to other treatments. Extract of Mexican sunflower could replace organic manure and rice husk charcoal as the nutrient source in banana seedlings. AbstrakPada saat pembibitan, komposisi media memiliki peranan penting terhadap pertumbuhan dan perkembangan bibit tanaman. Ekstrak bunga matahari Meksiko (Tithonia diversifolia) dapat menjadi pilihan yang cocok untuk menggantikan peran dari pupuk kandang dan sekam bakar pada fase pembibitan. Tujuan dari penelitian ini adalah untuk mempelajari kemampuan dari ekstrak bunga matahari Meksiko untuk menggantikan peran pupuk kandang dan sekam bakar sebagai sumber nutrisi bagi bibit pisang. Penelitian ini dilaksanakan dengan menggunakan Rancangan Acak Lengkap dengan tujuh perlakuan dan tiga ulangan. Ekstrak bunga matahari Meksiko dapat meningkatkan pertumbuhan tanaman (tinggi tanaman, jumlah daun, dan luas daun), biomassa tanaman, dan property dari kimia tanah (pH (H2O), karbon  organik  dan   total nitrogen). Aplikasi dari ekstrak bunga matahari Meksiko dua kali seminggu ke media tanah menghasilkan tinggi tanaman, jumlah daun, luas daun dan total biomassa tanaman yang lebih baik dibandingkan perlakuan lainnya. Hasil ini menunjukkan bahwa ekstrak bunga matahari Meksiko dapat menggantikan peran dari pupuk kandang sapi dan sekam bakar sebagai sumber nutrisi bagi bibit pisang.           


2015 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Fahrizal Hazra

<p>ABSTRACT</p><p>Snake fruit [Salacca zalacca (Gaertner) Voss] Pondoh is one of important fruits in Indonesia. The objective of the study was to determine the  influence of some selected potential microbes such as Phosphate Solubilizing Bacteria, Cellulolytic Bacteria, and  N2-fixing Bacteria (Azospirillum)  ongrowth of seedling  of  snake  fruit  Pondoh.  A  study  was  conducted  in  the nursery. The  experiment used  a  completely  randomized  design  with  treatments namely:  K0  (without  inoculum);  A1 (Azospirillum inoculum dose 5 ml), A2 (inoculum 25 ml); F1 (BPF inoculum dose 5 ml), F2 (dose 25 ml); S1; BPS inoculums (dose 5 ml), S2 (dose 25 ml); C1 (mixed bacterial inoculum A+F+S dose 5 ml), and C2 (A+F+S dose 25 ml). Plant height, P, C/N soil ratio, N were measured in 10 months for observing the impact of treatments. Results showed that the effect of inoculation of A2 to the amount of  N-total  (0.99%)  was significantly  different  compared  to  soil  with  K0;  and  not significantly different  from  the  amount  of  N-total  inoculation  A1,  F1,  F2, S1,  S2,  C1,  and  C2.  F2  increased availability of P (20.79 ppm) in the soil higher than  K0, A1, F1; and not significantly different from A2, S1, S2, C1, and C2.  K0 treatment gave  C/N ratio of soil significantly different from A1, A2, F1, F2, S1, S2, C1 and C2; BPS which showed activity in overhauling C-organic in the soil. Inoculation A2  and F2  increased  plant  height  15.48% and 9.31%  higher  than  the  control.  Inoculation  of  C2 differed  significantly  in improving  the  fresh  weight  compared  to  control;  and  not significantly different  from  A1,  A2,  F1,  F2,  S1,  S2,  and  C1  and  able  to increase  the  dry  weight  of  71.12% compared to the control.</p><p>Keywords: Azospirillum, Cellulolytic Bacteria, Phosphate Solubilizing Bacteria, Snake Fruit</p><p> </p><p>ABSTRAK</p><p>Salak Pondoh [Salacca zalacca (Gaertner) Voss] merupakan tanaman buah bernilai tinggi dan diminati  masyarakat.  Budidaya  salak  diawali  dengan pembibitan.  Penelitian  ini  bertujuan  menguji pengaruh  bakteri  potensial hasil  isolasi  dan  seleksi  dari  bakteri  pelarut  fosfat  (BPF), bakteri perombak  selulosa  (BPS),  dan  bakteri  pemfiksasi  N2  (Azospirillum) dalam  pertumbuhan  bibit tanaman  salak pondoh. Penelitian ini menggunakan rancangan acak lengkap satu faktor yang terdiri atas: K0 (tanpa inokulum/Kontrol);  A1  (inokulum  Azospirillum  5  ml), A2 (25  ml inokulum);  F1 (inokulum BPF dosis 5 ml), F2 (dosis 25 ml); S1 (inokulum BPS dosis 5  ml), S2 (dosis 25 ml); C1 (inokulum campuran A+F+S dosis 5 ml), dan C2 (dosis 25 ml).  Pengamatan dilakukan selama 10 minggu  sampai  bibit salak  Pondoh  memiliki dua daun.  Hasil penelitian menunjukkan  inokulasi A2dan S2  nyata  jumlah N-total (0.99%) di dalam tanah dibandingkan  dengan K0; tetapi  tidak berbeda nyata dibandingkan dengan perlakuan lain. Inokulasi F2 nyata meningkatkan P tersedia (20.79 ppm) di dalam tanah dibandingkan dengan K0, A1, F1; dan tidak berbeda nyata dengan A2, S1, S2, C1, dan C2. Induksi dengan seluruh isolat nyata terhadap C/N rasio tanah; yang menunjukkan adanya aktivitas BPS dalam  merombak C-organik  di dalam tanah.  Inokulasi  A2  dan  F2  mampu meningkatkan tinggi tanaman 9.31% dan 15.48% lebih  besar  dibandingkan kontrol. Inokulasi C2 nyata meningkatkan bobot basah bibit salak pondoh dibandingkan kontrol; tetapi tidak berbeda nyata dengan perlakuan lain serta mampu meningkatkan bobot kering 71.12% dibandingkan kontrol.</p><p>Kata kunci: Azospirillum, bakteri pelarut fosfat, bakteri perombak selulosa, Salak Pondoh</p>


2020 ◽  
Vol 21 (1) ◽  
pp. 40-48
Author(s):  
Muhammad Asril ◽  
Yuni Lisafitri

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam 


2021 ◽  
Vol 2 (2) ◽  
pp. 176-187
Author(s):  
Kingsley Uzoma ◽  
Michael E. Nkereuwem ◽  
Uzaifa K. Adamu ◽  
Adeniyi O. Adeleye ◽  
Abe Mololuwa

This study was carried out to evaluate the effect of mycorrhizal inoculation on the growth and nutrients uptake of maize planted in crude oil-contaminated soil. About 10 kg sterilized topsoil was contaminated with Bonny light crude oil (BLCO) at different concentrations: 0, 200, 300, and 500 ml/pot. Maize was sown at three seeds per pot and later thinned to two stands per pot after two weeks. Inoculation in treatments containing AM consisted of 20 g of Glomus clarum. Data on residual TPH content of the soil, plant height, number of leaves, fresh and dry weights, and nutrients uptake were collected at 4, 8, and 12 weeks after sowing (WAS). The 2 x 4 factorial experiment was laid in a completely randomized design and replicated 3 times. The results obtained indicated that AM inoculated maize had higher and significantly (p˂0.05) nitrogen, phosphorus, potassium, calcium, plant height, number of leaves, fresh and dry weights. Significantly (p˂0.05) lower residual TPH content was recorded with treatments inoculated with AM fungi compared to non-AM fungi inoculated treatments. AM colonization resulted in enhanced nutrients uptake thus, improving the physiological parameters of the test crop.


Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1412
Author(s):  
Antonio J. Carpio ◽  
María-Auxiliadora Soriano ◽  
José A. Gómez ◽  
Francisco S. Tortosa

Cover crops can be an effective means to protect soil and reduce risks of erosion in olive groves. However, for this protection to be significant, the vegetation must attain a significant amount of ground cover, which is estimated to be at least 30% during the rainy season. In olive groves on degraded soils, which occupy large surface areas in the olive-growing areas of the Mediterranean region, the establishment of cover crops may be an arduous challenge, particularly in areas with a high density of rabbits. In this study, we have selected two olive orchards with scarce natural vegetation located in Andalusia (southern Spain), in which rabbit populations intensively forage the cover crops, to test whether the self-seeding of an unpalatable species corn chamomile (Anthemis arvensis L.; A. arvensis for short) could achieve sufficient coverage for soil protection, in the year following that in which the broadcast-seeding was carried out for the implementation of cover crops. The hand broadcast-seeding of A. arvensis was carried out on sixteen elementary plots in the lanes of the two olive orchards in the autumn of 2015, and seed germination in the subsequent self-seeding took place in the autumn of 2016. The plant height and A. arvensis ground cover in these plots were measured throughout the two growth cycles, and aerial biomass was measured at maturity. The results showed that there were no significant differences in the maximum plant height between the two growth cycles (mean ± SD of 21.2 ± 1.6 cm), while the ground cover was significantly greater in the case of self-seeding, especially during the winter (37.2 ± 8.1 and 9.3 ± 6.7% for self-seeding and broadcast-seeding, respectively), and aerial biomass at maturity had more than doubled (99.7 and 43.9 g m−2, respectively). These data suggest that this unpalatable species could establish an effective herbaceous cover by means of self-seeding in olive groves on degraded soils that are being overgrazed owing to the high pressure of rabbits. Despite the poor establishment in the broadcast-seeding year, our findings indicate that A. arvensis might be an alternative cover crop that could help the sustainability of these threatened olive groves. Its high seed production (2000 to 4000 seeds per plant), and an early emergence just after the first autumn rains, should result in an increased ground cover by A. arvensis during the rainy season in the subsequent years of self-seeding. This, therefore, could contribute to soil conservation, in addition to providing other benefits of increased biodiversity and improvement for agricultural landscapes.


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