scholarly journals Effect of Alcoholic Catechin Extract on Hyperglycemia, Hyperlipidemia and Liver Functions in Alloxan Diabetic Mice

2014 ◽  
Vol 11 (3) ◽  
pp. 1192-1200
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Glucose ◽  
Diabetic Control ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Diabetic Mice ◽  
Diabetic Control Group ◽  
Liver Functions ◽  
Group A ◽  
Serum Biochemical ◽  
Group B

The objective of this study is to estimate the effect of the hydro-ethanolic catechin extract toward blood glucose, lipid profile and liver functions in alloxan diabetic mice. 50 healthy mice (25-30 g) were divided into five groups of ten animals for each. Group A received normal saline as normal control group. To induce diabetes, alloxan (150 mg/kg), intraperitoneal (i.p.) single dose was injected to groups B, C, D and E. Group B represents diabetic control group. Groups C, D and E received ethanolic catechin extract (30 mg/kg and 40 mg/kg) for different periods of 1, 2 and 3 weeks as treated groups. Blood glucose, serum lipids [Total Cholesterol (TC), Triglycerides (TGs) and High Density Lipoproteins (HDL)], asparagine transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were estimated after one, two and three weeks. Group B showed a significant increase in blood glucose, TC, TGs, AST, ALT and ALP as compared to group A. Groups C, D, and E showed a significant decrease in mentioned serum biochemical parameters in comparison to group B. In contrast, groups C, D and E showed significant increase in serum HDL as compared to B group. The results clearly revealed that ethanolic catechin extract possesses significant antihyperglycemic and antihyperlipidemic activities together with its ability to improve liver functions in alloxan diabetic mice.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

scholarly journals Effect of Fenugreek (Methi) on the Thymic volume in Streptozotocin-induced Diabetic Rats

1970 ◽  
Vol 16 (1) ◽  
pp. 1-4
Author(s):  
DK Mondal ◽  
MM Karim ◽  
S Mohal ◽  
BMA Yousuf ◽  
KM Shamim
Keyword(s):  
Body Weight ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Final Body Weight ◽  
Diabetic Control Group ◽  
Group A ◽  
Group B

Effect of fenugreek (Trigonella foenumgraecum) on thymic volume was studied in 30 long Evansrats. Among the total, 10 were treated with only vehicle (non-diabetic control - group A); 10 weretreated with streptozotocin only (diabetic control - group B) and 10 were treated with fenugreek(fenugreek treated - group C). Mean relative thymic volumes were 0.22210.018 ml/100 gm ofcorresponding final body weight (Mean 1 SEM), 0.14710.015 and 0.19910.020 in group A, B and Crespectively. In non-diabetic control group the thymic volume was significantly higher (p=0.005)than that of diabetic control group. In fenugreek treated diabetic group the volume was alsosignificantly higher (p=0.05) from that of diabetic control group and very much nearer to nondiabeticcontrol group (p=0.398). Fenugreek may preserve the thymic volume considerably indiabetic rats.doi: 10.3329/taj.v16i1.3892TAJ June 2003; Vol.16(1): 1-4

scholarly journals Effect of ropivacaine on peripheral neuropathy in streptozocin diabetes-induced rats through TRPV1-CGRP pathway

2019 ◽  
Vol 39 (11) ◽  
Author(s):  
Nanwen Zhang ◽  
Haixiang Wei ◽  
Weifang Wu ◽  
Peimin Lin ◽  
Yuan Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Peripheral Neuropathy ◽  
Conduction Velocity ◽  
Nerve Conduction Velocity ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Control Group ◽  
Nondiabetic Control ◽  
Group A ◽  
Group B

Abstract Objective To determine the effect of ropivacaine on peripheral neuropathy in diabetic rats and its possible mechanism. Methods Forty-eight Sprague–Dawley rats were randomly divided into six groups: nondiabetic control group, nondiabetic group A (0.25% ropivacaine), nondiabetic group B (0.75% ropivacaine), diabetic control group (diabetic peripheral neuropathy (DPN) +artificial cerebrospinal fluid), diabetic group A (DPN+0.25% ropivacaine), and diabetic group B (DPN + 0.75% ropivacaine), with eight rats in each group. Within an hour of the last administration, the sciatic motor nerve conduction velocity (MNCV) of each group was measured, and the morphological changes of rat sciatic nerve were observed by HE, Weil’s staining and electron microscopy. The expression of transient receptor potential vanilloid (TRPV1) in the spinal cord dorsal horn of rats was analyzed by immunohistochemistry, and the expression of Calcitonin gene-related peptide (CGRP) protein in the spinal cord was analyzed by Western blot. Results Compared with the nondiabetic control group, elevated blood glucose, decreased weight and reduced average mechanical withdrawal threshold (MWT), additionally, the sciatic nerves showed significantly slowed conduction velocity (both P<0.001) and damaged pathological structure, the expression of TRPV1 and CGRP were decreased (both P<0.001) in the diabetic groups. Compared with the diabetic control group, down-regulation of TRPV1 and CGRP in spinal cord was significant for the diabetic groups A and B treated with 0.25 and 0.75% ropivacaine, the higher concentration of ropivacaine correlated with a greater change. Conclusion Ropivacaine can significantly block sciatic nerve conduction velocity in DPN rats in a concentration-dependent manner, which may be related to the expression of the TRPV1-CGRP pathway.

scholarly journals Ameliorative Effect of Chlorophyllin on Oxidative Stress in Experimental Model of Diabetes

2017 ◽  
Vol 8 (4) ◽  
pp. 506 ◽  
Author(s):  
Abani K Patar ◽  
Surya Bhan ◽  
Donkupar Syiem ◽  
Anupama Sharma
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Diabetic Control ◽  
Protein Carbonyl ◽  
Control Group ◽  
Diabetic Mice ◽  
Ultrastructural Studies ◽  
Ameliorative Effect ◽  
Group A ◽  
Group B

The aim of this present study was to investigate the effect of chlorophyllin (CHL) on oxidative stress in Streptozotocine (STZ) induced diabetic mice. For the study, mice were divided into Group A: normal control, Group B: diabetic control, Group C: diabetic mice treated with the ascorbic acid, and Group D: diabetic mice treated with CHL. Levels of Reactive Oxygen Species (ROS), lipid peroxidation, protein carbonyl, superoxide dismutase (CuZn SOD &amp;Mn-SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were examined in kidney and heart tissues of different experimental groups. Histological and ultrastructuralstudies were also carried out to evaluate any changes in tissues as well as sub-cellular organelles. ROS, lipid peroxidation, and protein carbonyl levels have been significantly decreased with concomitant increased of CuZn SOD, Mn-SOD, CAT, GPx, and GR activity in CHLtreated diabetic mice. The histological and ultrastructural studies showed that CHL attenuates the detrimental effect of oxidative stress and alleviated tissue injuries in STZ induced diabetic mice. These results suggested that CHL possesses antioxidative activity and has the potential to amelioratediabetes-associated oxidative stress in mice.

scholarly journals Comparative Study of the Effect of Ethanol Extract of Psidium Guajava Linn Leaves with Glibenclamide on Experimentally Induced Diabetes Mellitus in Rats

2017 ◽  
Vol 5 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Azmary Momtaz ◽  
Khaleda Sharmin ◽  
Sharmin Rahman ◽  
Nashid Sultana ◽  
Rayhana Sharmin
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group A ◽  
Group B ◽  
Experimentally Induced

Background: Diabetes Mellitus is a major health problem not only in urban but also in the rural areas of Bangladesh. Regarding its treatment, a suitable drug is yet to be available which can permanently cure this disease. Over 400 traditional plants have been reported for the treatment of diabetes, but only a small number have received scientific and medical evaluation to assess their efficacy.Objective: The study was performed to compare the anti diabetic effect of Psidium guajava Linn leaves with an oral anti diabetic drug (Glibenclamide), in experimentally induced diabetic rats.Materials and method: The experiment was carried out in the department of Pharmacology & Therapeutics of Dhaka Medical College, Dhaka, Bangladesh from July 2012 to June 2013. Twenty four healthy rats of Long Evans Norwegian strain were divided into 4 groups (group A, B, C and D) comprising 6 rats in each. Group A (normal control) received standard rat food for 14 days. Diabetes was induced by administration of Alloxan 120 mg/kg/body weight in group B, C and D. Group B (diabetic control group) was given standard rat food. Group C and D was treated with ethanol extract of Psidium guajava leaves 100 mg/kg body weight and Glibenclamide 1.5 mg/kg/day orally respectively. Total duration of the experiment was 15 days.Results: Administration of ethanol extract of Psidium guajava leaves in group C and Glibenclamide in group D produced a significant reduction (p<0.001) in blood glucose level as compared to group B (diabetic control group).Conclusion: Ethanol extract of Psidium guajava leaves can be considered as a potential anti diabetic agent like Glibenclamide but it requires further investigations.Delta Med Col J. Jan 2017 5(1): 9-14

scholarly journals Antidiabetic and Wound Healing Effects of Smeathxanthone A

2016 ◽  
Vol 02 ◽  
pp. 5 ◽  
Author(s):  
David Emery Tsala ◽  
Alain Meli Lannang ◽  
Theophile Dimo ◽  
Solomon Habtemariam ◽  
Jean Arnaud Ekanga ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Breaking Strength ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Tissue Healing ◽  
Diabetic Control Group

The purpose of this study was to investigate whether smeathxanthone A isolated from Garcinia smeathmaniiimproves incisional wound healing in diabetic mice. Male albino alloxan-induced diabetic mice (n= 20) were divided into five groups: normal control, diabetic control, 2.5 mg/kg glibenclamide given orally, 0.05 and 0.1 mg/kg smeathxanthone A given subcutaneously. Animals were euthanized on postoperative day 10 after wounding; body weight, blood glucose, breaking strength, and histologic examination were reviewed. Smeathxanthone A significantly increased skin tensile strength (24% higher than diabetic control group when given at 0.1 mg/kg), stimulated hair growth, and reduced signs of inflammation in the scar sections. Smeathxanthone A also reduced blood glucose levels in diabetic mice (45% higher than diabetic control group when given at 0.1 mg/kg). The present study demonstrates that administration of smeathxanthone A after laparotomy expedites wound healing in mice. We suggest that it could confer benefits to tissue healing by significantly enhancing tissue collagen deposition and controlling blood glucose levels.

scholarly journals ANTI-HYPERGLYCEMIC EFFECT OF DIFFERENT FRACTIONS OF ANNONA RETICULATA LEAF

2016 ◽  
pp. 256
Author(s):  
Soumya Prakash Rout ◽  
Durga Madhab Kar ◽  
Laxmidhar Maharana
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Ethyl Acetate Fraction ◽  
Diabetic Control ◽  
Control Group ◽  
Alcoholic Extract ◽  
Diabetic Control Group ◽  
Annona Reticulata

<p>ABSTRACT<br />Context: Several species of the genus Annona were reported to have hypoglycemic properties and this makes Annona reticulata Linn. (Annonaceae)<br />an interesting plant for investigating its anti-hyperglycemic potential.<br />Objective: Different fractions prepared from hydro-alcoholic extract of A. reticulata leave were investigated for their blood glucose lowering effect on<br />Streptozotocin (STZ) induced hyperglycemic rats.<br />Methods: Ethyl acetate, methanol, and residual fractions (at dose level of 100 mg/kg by oral route) prepared from the hydro-alcoholic extract of<br />A. reticulata leave were administered for 14 consecutive days to STZ induced hyperglycemic rats for evaluation of their anti-hyperglycemic potential.<br />Anti-hyperglycemic potential was assessed by observation of a decrease in fasting blood glucose level.<br />Results: The studies revealed that ethyl acetate fraction decreased the blood glucose level of hyperglycemic rats from 447.67 to 234.17 mg/dL and is<br />significant (p&lt;0.001) when compared with diabetic control group. The residual fraction and methanolic fraction decreased blood glucose level from<br />417.83 to 402.50 mg/dL and 432.33 to 371.67 mg/dL respectively but not significant when compared with the diabetic control group. Standard drug<br />metformin (dose 300 mg/kg) reduced the blood glucose level from 447.33 to 219.50 mg/dL.<br />Discussion: Ethyl acetate fraction at tested dose level was capable not only to control the elevated blood glucose level but also able to attenuate<br />certain secondary parameters associated with STZ induced hyperglycemia.<br />Conclusion: This study suggested that the ethyl acetate fraction prepared from hydro-alcoholic extract of A. reticulata leave exhibit potential antihyperglycemic<br />property<br />in the tested<br />experimental<br />models and should be investigated<br />further.<br />Keywords: Streptozotocin, Diabetes, Dyslipidemia.</p>

scholarly journals Preventive Role of Zingiber Officinale against Hyperglycemia in Alloxan Induced Diabetic Rats

2019 ◽  
Vol 30 (Number 1) ◽  
pp. 26-29
Author(s):  
S Sultana ◽  
N Y Mili ◽  
R Afroz ◽  
S Parveen
Keyword(s):  
Blood Glucose ◽  
Zingiber Officinale ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Group Iii ◽  
Diabetic Control Group ◽  
Glucose Estimation ◽  
Preventive Role

The experimental animal study was undertaken to investigate the preventive role ginger juice against hyperglycemia in alloxan induced diabetic rats.Male wistar rats,(130-150)gm wt fed on standard diet and water ad libitum, were divided into 3 groups(n=6) in each group: Group-L non-diabetic control group, Group-II, diabetic control & Group-III, normal rats pretreated with ginger before they were made diabetics. Diabetes was induced by inj. alloxan 150mg/kg body wt.,tp (Group-IL on 2nd day & Group-Ill, on the 9th day).Rats having blood glucose level of more than 7mmol/L on day 5(72 hours after alloxan inj) were considered diabetic & selected for experiment. Rats of Group-Ill received Zingiber officinale (ginger juice) (4m1/kg.body,wt orally) for 7 days (day 2-day8) through Pyles tube before alloxan induction & 3days after the induction. On day 12, animals were sacrificed under light ether anaesthesia, blood was collected by cardiac puncture for blood glucose estimation. Pretreatment with Zingiber officinale (ginger) juice significantly (p<0.01) reduced alloxan induced hyperglycemia.Zingiber officinale (ginger) is one of the most widely used spices and is reputed to have medicinal properties against diabetes mellitus. This study suggests that pretreatment with Zingiber officinale(ginger) prevents the development of hyperglycemia in alloxan induced diabetic rats.

scholarly journals Evaluation of the oral hypoglycemic activity of methanolic extract of Garcinia indica seeds in streptozotocin induced diabetic Albino rats

2018 ◽  
Vol 7 (6) ◽  
pp. 1147
Author(s):  
Padmaja Shetty K. ◽  
Pushpa V. H.
Keyword(s):  
Blood Glucose ◽  
Diabetic Control ◽  
Hypoglycemic Activity ◽  
Control Group ◽  
Glucose Levels ◽  
Garcinia Indica ◽  
Group 4 ◽  
Blood Glucose Levels ◽  
Diabetic Control Group ◽  
Group 5

Background: Diabetes mellitus is a multifactorial metabolic disorder with several microvascular and macrovascular complications. Several plants have been used as dietary adjuvants to conventional drug therapy. Garcinia indica exhibits significant hypolipidemic and hypoglycemic activity. This study was conducted to evaluate the hypoglycemic effects of methanolic extract of seeds of Garcinia indica on blood glucose levels in Streptozotocin induced diabetic albino rats.Methods: Five groups of wistar albino rats (n=6) weighing 150-200g of either sex aged 3-4 months were obtained for the study. After overnight fasting, streptozotocin (50mg/kg) was administered intraperitoneally to induce diabetes. Five groups are: Group-1: Non diabetic control group, Group-2: diabetic control, Group-3: diabetic standard, Group-4: test group, Group-5: half of test + half of standard. Fasting blood sugar was estimated on 1, 3, 7, 14 and 28th day by capillary blood glucose method. The data obtained were subjected to statistical analysis.Results: In this study, following Streptozotocin administration the blood glucose levels increased in all groups on day 0. In group 2, blood glucose level gradually increased to 445.6±1.75mg/dl over a period of 4 weeks. Following glibenclamide administration in Group 3 - there was a gradual reduction in blood glucose levels: 269.8mg/dl - day 7 to 101.8mg/dl - week 4. Group 4 - persistent and significant (p<0.05) fall in blood glucose levels reaching upto 107mg/dl at the end of 4 weeks. Group 5 - 330mg/dl on day 1 which significantly (p<0.05) reduced to 101mg/dl on day 28. There was improvement in weight in group 4 and group 5 diabetic rats.Conclusions: The extract alone and in combination with glibenclamide showed significant hypoglycemic activity in comparison to diabetic control group.

scholarly journals Pharmacodynamics Drug Interactions of Metformin with Aspirin and Nifedipine

2016 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Khidir A. M. Hassan ◽  
Mahmoud M. E. Mudawi ◽  
Mansour I. Sulaiman
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Control ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Citrate Buffer ◽  
Treatment Groups ◽  
Diabetic Control Group

Metformin is now being recognized as the standard therapy in T2D patients who are overweight. Metformin has many drug-disease interactions that can increase the risk of metformin-associated lactic acidosis. Therefore this study was conducted to evaluate any possible pharmacodynamic interactions between metformin and drugs used to treat chronic diseases e.g. Hypertension. The rats were fasted overnight before inducing diabetes with streptozotocin. The rats were given an intraperitoneal injection of streptozotocin (50 mg kg−1) freshly prepared in 0.1M sodium citrate buffer. The diabetic state was confirmed 72 h after streptozotocin injection. Diabetic rats were grouped into seven groups each group of five rats and distributed among the normal control group diabetic control group and the treatment groups. The treatment continued for 10 days. Blood samples were taken before treatment and after 10 days and analyzed for serum glucose, cholesterol, HDL, LDL, and triglycerides. In the diabetic control group which was given STZ alone the blood glucose level decreased significantly (p &lt; 0.05) after 10 days but still above the hyperglycemic level (200mg/dl). The same was observed in the group treated with metformin. The group treated with nifedipine and aspirin showed significant reduction (p &lt; 0.01) in the glucose level below the hyperglycemic level (200mg/dl). While the groups treated with (Metformin + Nifedipine) and (Metformin +Aspirin) showed highly significant reduction (P&lt;0.001) in blood glucose level. These results conclude that the combination of (metformin +Nifedipine) and the combination of (Metformin + Aspirin) have highly significant hypoglycemic effect. It also showed that Nifedipine has promising role in reducing blood glucose level, lipid profile especially LDL-cholesterol, and body weight.

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