scholarly journals Study the effect of stable magnetic field on the inhibition activity of Saccharomyces boulardii against pathogenic bacteria isolated from urinary tract infection

2017 ◽  
Vol 14 (3) ◽  
pp. 455-460
Author(s):  
Baghdad Science Journal
Keyword(s):  
Magnetic Field ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Inhibitory Action ◽  
Pathogenic Bacteria ◽  
Health Benefit ◽  
Saccharomyces Boulardii ◽  
Diffusion Method ◽  
Micro Organisms

An increasing trend to use probiotic and study their effects on the pathogens has been conductor where they are defined as live micro-organisms that give a health benefit to the host when ingested in sufficient quantities, including the yeast Saccharomyces boulardii In addition research show that a magnetic field (MF) has a biological effect. This study aims to investigate the effects of magnetic field on the inhibitory action of Saccharomyces boulardii against bacteria isolated from urinary tract infection, Study the sensitivity of bacterial isolates to antibiotics after diagnosis by microscopic, Cultural and biochemical examinations as well as Api20 E examinations were used gram negative bacteria , Most isolates were resistant to antibiotics elected, using Well diffusion method to investigate the inhibitory action of suspension and supernatant of the yeast with and without magnetic field in four different intensities (200-300-400-500) milli Tesla. The results show that inhibitory efficacy of each was higher wish increasing the magnetic field strength and differed in their impact depending on the type of bacteria and the isolate of K.pneumoniea was the most sensitive in all experiments, and suspension was more effective.

scholarly journals THERAPEUTIC ROLE OF AZADIRACHTA INDICA LEAF EXTRACT AGAINST UTI (URINARY TRACT INFECTION)

2020 ◽  
pp. 5-7
Author(s):  
K. Dinesh Singh
Keyword(s):  
Silver Nanoparticles ◽  
Urinary Tract Infection ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Tract Infection ◽  
Azadirachta Indica ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Micro Organisms

Azadirachta indica is a multipurpose tree with multiple health benefits. Different parts of the plant are shown to exhibit antimicrobial effects against a wide variety of micro organisms. In the present study we investigate the antibacterial activity of Azadirachta indica extract against UTI (urinary tract infection) causing bacteria. Silver nanoparticles could be synthesized using the leaves of Azadirachta indica as reducing agent. The resulting silver nanoparticles were characterized using UV-Vis spectrophotometer, X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and Fourier Transformations Infra Red (FTIR) spectroscopic analysis. The antibacterial activity was assayed by Agar well diffusion method using 20µl each of sterile Nutrient Agar (NA) (Hi-Media) and Potato-Dextrose Agar (PDA). The resulting silver nanoparticles exhibited best inhibitory activities against Escherichia coli and Proteus mirabilis which are main causing agents for UTI.

Antimicrobial Activity of Herbal Mixture Extract Combination on Microorganisms Isolated from Urinary Tract infection

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1346-1351
Author(s):  
Risala H Allami ◽  
Raghad S. Mouhamad ◽  
Sura A. Abdulateef ◽  
Khlood abedalelah al-Khafaji
Keyword(s):  
Urinary Tract Infection ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Klebsiella Spp

Urinary tract infection (UTI) is the second most common infection after respiratory tract infection. Its prevalence is more in women as compared to men. Approximately 50% of women have an infection of the Urinary tract in their life-time. The bacterial infection is one of the most important bioactivity; using their ability to imitate evanish then distributes international fitness problems into the 21st centenary. Thus a recent study was undertaken to investigate the antibacterial activity of a mixture of three medicinal plants against UTI infectious isolates. The three considered plants were (Aloe vera, Artemisia herba alba and Teucrium polium), which were used in Iraqi medicine for many centuries. The effectiveness of this combination was investigated using in vitro well diffusion method. The extract was tested against four isolated pathogenic bacteria (Staphylococcus aureus, Klebsiella spp, and Proteus spp). The aqueous extract exhibited antibacterial activity against gram-positive and gram-negative bacteria. The mixture extract had the highest effect against S. aureus and Proteus spp, followed by a lower effect on Klebsiella spp. In conclusion, the antibacterial effect of the tested plant extracts confirmed a higher impact on Gram-positive bacteria as compared to Gram-negative bacteria. Therefore, it can be concluded that the usage of these plants as a traditional medicine form can be considered as a strong assistant to regular medicine drugs and treatments.

scholarly journals Bacteriological Profile and Antimicrobial Susceptibility Patterns of Urine Culture Isolates from Patients in a Tertiary Care Centre in Lalitpur

2020 ◽  
Vol 5 (1) ◽  
pp. 881-885
Author(s):  
Ruchee Manandhar ◽  
Bijendra Raj Raghubanshi ◽  
Moni Mahato ◽  
Sweekrity Neupane ◽  
Rajni Lama
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Pathogenic Bacteria ◽  
Tertiary Care ◽  
Urine Samples ◽  
Diffusion Method ◽  
Microbiological Methods ◽  
Tract Infections

Introduction: Urinary tract infection (UTI) is a microbial infection of the urinary system involving the urinary tract anywhere from kidney to urethra. It is one of the most common bacterial infections affecting men and women in developing countries with a high rate of morbidity and financial cost. Objectives: The objective of our study is to study the prevalence of UTI in patients attending KIST Medical College & Teaching Hospital (KISTMCTH), and determine the antimicrobial susceptibility pattern of bacteria thus isolated. Methodology: A total of 3742 urine samples from patients suspected of urinary tract infections presenting with the history and symptoms suggestive of urinary tract infection, attending various departments of KISTMCTH from April 2017 to April 2018 were studied. Isolates were identified by standard microbiological methods and tested for in vitro antibiotic susceptibility by modified Kirby-Bauer disc diffusion method. Results: Pathogenic bacteria were isolated from 646 out of 3742 urine samples (17.26%). Escherichia coli was the most common bacteria isolated (67.02%) followed by Klebsiella pneumoniae (14.5%). Other bacteria isolated were Enterococcus spp, Pseudomonas aeurigenosa, Klebsiella oxytoca, Proteus mirabilis, Satphylococcus aureus, Coagulase negative staphylococcus spp, Proteus vulgaris, Acinetobacter spp, Citrobacter freundii, Citrobacter diversus and Enterobacter spp. The isolated pathogenic bacterias were most resistant to Ampicillin (46.43%) and least resistant to Imipenem (6.03%). However, the first line antibiotic the bacterias were least resistant to was cefotaxime (7.12%). Conclusions: Marked resistance has been observed with commonly prescribed antibiotics like Ciprofloxacin and Norfloxacin. Therefore, studies should be conducted routinely to identify the common bacteria causing UTI and formulate appropriate antibiotic policy.

scholarly journals Clinicoetiological profile of urinary tract infection in pediatric population in a teaching hospital in south India

2021 ◽  
Vol 8 (12) ◽  
pp. 1958
Author(s):  
Poornima Venugopal ◽  
Carol Sara Cherian ◽  
Pooja Raghunath
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Teaching Hospital ◽  
Bacterial Infections ◽  
Clinical Presentation ◽  
Diffusion Method ◽  
Male Predominance ◽  
Causative Microorganism ◽  
Wide Range

Background: Urinary tract infection (UTI) is one of the most common bacterial infections seen in children. A wide range of organisms with varying antibiotic sensitivity patterns have been known to cause UTI. The objective of the study was to analyse the clinical presentation of UTI in children between 1-month to15 years of age and to analyse the causative microorganism and their drug susceptibility in UTI in children between 1 month to 15 years of age.Methods: A retrospective study was conducted in a teaching hospital in Kerala, between July 2018 and June 2020 among children 1 month to 15 years of age who presented with symptoms of UTI. Patients who had culture positive UTI were included in the study. Clinical data was obtained from inpatient and outpatient records. Antimicrobial susceptibility was done for positive urine culture by Kirby-Bauer disk diffusion method. Statistical analysis was done using Statistical package for social sciences (SPSS) software version 16.Results: Of the 1057 urine samples analysed, 18.44% had significant bacteuria. 43.07% were children less than one year of age with male predominance. Fever and dysuria were the most common clinical presentation. E. coli was the most prevalent pathogen isolated followed by Klebsiella pneumoniae. Enterococcus faecalis was the only gram-positive bacilli isolated. Highest resistance was shown to ampicillin, third generation cephalosporins and co-trimoxazole. Least resistance was shown to nitrofurantoin, fluoroquinolones, aminoglycosides, piperacillin- tazobactam and carbapenems.Conclusions: Regular surveillance programme is necessary for implementation of guidelines for empiric treatment of UTI.

ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS AGAINST MULTI DRUG RESISTANT URINARY TRACT INFECTION CAUSING ORGANISMS

2021 ◽  
Vol 1 (6) ◽  
pp. 85-95
Author(s):  
Perumal G
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Medicinal Plants ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogens ◽  
Drug Resistant ◽  
Antibiotic Drug ◽  
Phytochemical Studies

The present study was isolate Bacterial pathogens form Urinary Tract Infection and identified the Bacterial pathogens from UTI patients. Determination of the antibiotic drug resistant pattern of the isolated pathogenic bacteria using standard antibiotic discs Ampicilin (25μg), Erithromycin (15μg), Chloramphenicol (10μg) Gentamicin (10μg) and Tetracycline (30 μg).The study was carried out, in vitro screening of ethanolic extracts of some medicinal plants against the bacterial pathogens Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Pseudomonas aeruginosawere isolate from the UTI. When compared with standard antibiotic disc selected plants extracts were showed maximum zone of inhibition against all the pathogens. This investigation strongly recommends that phytochemical studies are required to determine the types of compounds responsible for the antibacterial effect of these medicinal plants. Key words: Bacterial pathogens, Antibiotic drug resistant pattern and Medicinal plants

scholarly journals Antimicrobial susceptibility test of pathogens isolated from urinary tract infection suspected cases

2014 ◽  
Vol 2 (1) ◽  
pp. 28-34
Author(s):  
K Yadav ◽  
S Prakash ◽  
RC Serayi ◽  
T Shilpkar ◽  
S Shrestha
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Medical Problem ◽  
Medical Sciences ◽  
Medical College ◽  
Citrobacter Diversus ◽  
Effective Drugs

Background and objectives: Urinary tract infection (UTI) is associated with multiplication of organisms in urinary tract and is defined by the presence of more than 105 organisms per ml in a midstream sample of urine (MSU). UTI is most commonly acquired bacterial infection in ambulatory and hospitalized populations. E.coli is the most predominant organism to colonise the urethral meatus and perineum before ascending to the bladder. Drug resistance of pathogens is a serious medical problem, because of very fast arise and spread of mutant strains that are insusceptible to medical treatment of UTI. Therefore, this study was carried out to determine the common pathogenic bacteria causing UTI and to determine their antibiotic susceptibility pattern. Material and Methods: Mid stream urine samples of the UTI suspected pateints were collected in the Mid Stream Urine (MSU) samples were collected in the sterile clean dry wide mouthed bottle. Standard protocol was followed to isolate and identify organism which was followed by disc diffusion antibiotic sensitivity tests. Results: A total of 100 samples were collected. Out of 100 samples, 25 samples showed a significant growth E.coli, Citrobacter diversus, Klebsiella pneumoniae, Staphylococcus aureus were isolated. E.coli (84%) was found to be the most prevalent causing UTI. Conclusion: UTI was found more common in female than male and E.coli was found to be main causes of UTI. Ciprofloxacin showed more effective drugs in the treatment of UTI. DOI: http://dx.doi.org/10.3126/jmcjms.v2i1.11393   Janaki Medical College Journal of Medical Sciences (2014) Vol. 2 (1): 28-34

scholarly journals Urinary Tract Infection among Patients Visiting Ganesh Man Singh Memorial Hospital and Research Center, Lalitpur, Nepal

2019 ◽  
Vol 6 ◽  
pp. 108-112
Author(s):  
Janak Raj Dhungana ◽  
Aruna Budhathoki ◽  
Goma Poudel ◽  
Jyotika Basnet ◽  
Ravi Shah
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Sensitivity ◽  
Memorial Hospital ◽  
Diffusion Method ◽  
Gram Negative ◽  
Microbiological Methods ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Objectives: The objective of this study was to determine the prevalence of urinary tract infection (UTI) and antibiotic sensitivity pattern among the suspected UTI cases visiting at Ganeshman Singh Memorial Hospital Lalitpur, Nepal. Methods: A total of 300 mid-stream urine, catheter and suprapubic aspirate from UTI suspected patients were included and processed for routine microscopy and culture and then identified by standard microbiological methods. Antibiotic susceptibility test was performed by Kirby- Bauer disc diffusion method. Results: Out of 300 samples, 55(84.6%) mid-stream urine and 10(15.4%) catheter sample had significant bacterial growth. E. coli (32,49.2%) was the most common isolate followed by Staphylococcus aureus (10,15.3%), Enterobacter spp. (8,12.3%), Klebsiella spp. (7,10.7%), Pseudomonas aeruginosa (3,4.6%), Proteus spp.  (3,4.6%), Acinetobacter spp. (1,1.5%) and Enterococcus spp. (1,1.5%). Most of the Gram-negative bacterial isolates were sensitive to Ceftriaxone (88.8%) followed by Gentamicin (72.2%), and Nitrofurantoin (64.8%) and resistant to Amoxicilin (68.5%) followed by Nalidixic Acid (53.7%). Gram positive isolates were sensitive to Amikacin (72.7%) followed by Imipenem (63.6%) and Gentamicin (63.6%) whereas resistant to Amoxycilin (72.7%) and Ciprofloxacin (63.63%). Conclusion: The main cause of the UTIs was found as Gram negative bacteria. Prescription of antibiotics based on susceptibility tests would help in reduction of antibiotic resistance.

Detection of Virulence Factors and Antimicrobial Resistance in Enterococci Isolated from Urinary Tract Infection

2018 ◽  
Vol 4 (2) ◽  
pp. 30-34 ◽  
Author(s):  
Surovi Era Suchi ◽  
SM Shamsuzzaman ◽  
Bhuiyan Mohammad Mahtab Uddin ◽  
Md Abdullah Yusuf
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
Medical College

Background: The genus Enterococcus is of increasing significance as a cause of nosocomial infections and this trend is exacerbated by the development of antibiotic resistance.Objective: The aims of this study was to find out the susceptibility pattern and the prevalence of virulence genes in Enterococcus strains isolated from urinary tract infection in Bangladesh.Methodology: This cross-sectional study was conducted in the Department of Microbiology at Dhaka Medical College, Dhaka from January 2015 to December 2015 for a period of one (01) years. All the admitted patients in the Dhaka Medical College & Hospital, Dhaka and Banghabandhu Sheikh Mujib Medical University, Dhaka who were  presented with clinical features of urinary tract infection at any age with both sexes were included as study population. The antimicrobial susceptibility of the strains was determined using the disk diffusion method; vancomycin susceptibility pattern was detected by MIC method. PCR was performed for the detection of genus-species, vanA, vanB gene and potential virulence genes.Result: Out of 84 Enterococci, majority (71.42%) of the isolates were E. faecalis followed by 23.81% were E. faecium. Regarding antimicrobial susceptibility pattern, 100% isolated Enterococci were sensitive to vancomycin, linezolid and teicoplanin. Most of the Enterococci were resistant to ciprofloxacin and ceftriaxone (92.86%) followed by 88.10% to gentamicin and 85.71% to azithromycin and rifampicin. No VRE was identified and the range of MIC for vancomycin was 1-4 µg/ml. None of the Enterococci was positive for vanA and vanB genes. Out of total E. faecalis and E. faecium, 87.50% were positive for ebp and 77.5%, 72.5%, 55.0%, 45.0%, 40.0% and 10.0% of isolates were positive for gelE, esp, ace, cyl, asa and hyl respectively.Conclusion: Almost all the virulence genes were more prevalent in E. faecalis isolates.Bangladesh Journal of Infectious Diseases 2017;4(2):30-34

Sign in / Sign up

Export Citation Format

Share Document