scholarly journals CFIA-Turbidimetric and Photometric Determination of Vitamin B9 (Folic acid) Using LEDs as a Source of Irradiation and Two Solar Cells as an Energy Transducer

2017 ◽  
Vol 14 (4) ◽  
pp. 773-786
Author(s):  
Baghdad Science Journal
Keyword(s):  
Folic Acid ◽  
Classical Method ◽  
Standard Addition ◽  
Photometric Determination ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Simple Method ◽  
Vitamin B9 ◽  
Significant Difference

A specific, sensitive and simple method was used for the determination of: vitamin B9 (Folic acid) in pure and pharmaceutical formulations using continuous flow injection analysis. The method is based on formation of ion pair compound between folic acid and ammonium molybdate in an aqueous medium to obtain a gray precipitate complex, using homemade; Ayah-6SX1-ST-2D solar cell CFI Analyzer. Optimum parameters was studied to increase the sensitivity for developed method. The linear range for the calibration graph was 0.01-0.6 mMol.L-1 of vitamin B9 and LOD was 131.994 ng/sample with correlation coefficient ( r ) of 0.9810, RSD% was lower than 0.1%, (n=9) for the determination of vitamin B9 at concentration (0.07and 0.5) mMol.L-1 respectively. The developed method was applied successfully for the determination of vitamin B9 in pharmaceutical tablets. A comparison was made between two methods: developed method and the classical UV spectrophotometric method at ?max=255 nm, by using the standard addition method via the use of paired t-test. It showed that there was no significant difference between the developed method and the classical method for determination vitamin B9 at 95% confidence level.

scholarly journals Assessment of long distance chasing photometer (NAG-ADF-300-2) by estimating the drug atenolol with ammonium molybdate via continuous flow injection analysis

2020 ◽  
Vol 17 (1) ◽  
pp. 0078
Author(s):  
Elham N. Mezaal N. Mezaal
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Continuous Flow ◽  
Classical Method ◽  
Standard Addition ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Long Distance ◽  
Optimum Parameters

     Atenolol was used with ammonium molybdate to prove the efficiency, reliability and repeatability of the long distance chasing photometer (NAG-ADF-300-2) using continuous flow injection analysis. The method is based on reaction between atenolol and ammonium molybdate in an aqueous medium to obtain a dark brown precipitate. Optimum parameters was studied to increase the sensitivity for developed method. A linear range for calibration graph was 0.1-3.5 mmol/L for cell A and 0.3-3.5 mmol/L for cell B, and LOD 133.1680 ng/100 µL and 532.6720 ng/100 µL for cell A and cell B respectively with correlation coefficient (r) 0.9910 for cell A and 0.9901 for cell B, RSD% was lower than 1%, (n=8) for the determination of atenolol at concentration (0.5, 0.7 and 5) mmol/L respectively. The results were compared with classical method UV-Spectrophotometric at λ max=270 nm using the standard addition method via the use of t-test, at 95% confidence level. The comparison of data explain that long distance chasing photometer (NAG-ADF-300-2) is the choice with excellent extended detection and wide application.                                                                                                       

scholarly journals Newly developed method for the determination of sitagliptin phosphate using rubeanic acid as a precipitating agent via the use of ISNAG continues flow fluorimeter

2020 ◽  
Vol 11 (3) ◽  
pp. 3613-3625
Author(s):  
Nagam S.Turkey Al-Awadie ◽  
Kefah H. Ismael Al-Saadi
Keyword(s):  
Classical Method ◽  
Standard Addition ◽  
Calibration Graph ◽  
Physical Parameters ◽  
Pharmaceutical Drugs ◽  
Significant Difference ◽  
Rubeanic Acid ◽  
Detector Cell ◽  
Method Analysis

A new, a simple, sensitive and fast developed method for the estimation of sitagliptin phosphate in pure and pharmaceutical drugs (tablet). This method based on the reaction between sitagliptin phosphate and rubeanic acid to form reddish-black ppt, using ISNAG-Fluorimeter analyzer via the measure of diverged light at 90° from radiation source which is used a range of high intensity ultraviolet 184.9 nm & 253.7 nm by low-pressure mercury lamp while detector cell can detect at 410-1150 nm. The resultant of diverged light by an interaction between incident photons and precipitated particles giving rise to a longer wavelength that the detector can detected. Chemical and physical parameters were studied and optimized. The calibration graph was linear in the range of (0.03-13 ) mmol.L−1 , with correlation coefficient ( r )= 0.9881 ,linearity percentage r2%= 97.64 , L.O.D = 0.7848µg/sample and RSD lower than 0.6 % for (3&7 ) mmol.L−1 ( eight replicates) concentration of sitagliptin phosphate. This method was successfully applied for the determination of sitagliptin phosphate in two different companies of pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (UV-spectrophotometer by measuring of absorbance ) using standard addition method via the use of t-test, it was noticed that there was no significant difference between two methods at %95 confidence level.

scholarly journals New Fluorometric Method for the Determination of Ketotifen Fumarate Using Continuous Flow Injection Analysis via ISNAG-fluorimeter

2019 ◽  
Vol 16 (2) ◽  
pp. 0353
Author(s):  
Turkie Et al.
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Classical Method ◽  
Calibration Graph ◽  
Injection System ◽  
Minimum Concentration ◽  
Ketotifen Fumarate ◽  
Fluorescence Measurements ◽  
Significant Difference

        A newly developed analytical method was conducted for the determination of Ketotifen fumarate (KTF) in pharmaceuticals drugs via quenching of continuous fluorescence of 9(10H)-Acridone (ACD). The method was applied using flow injection system of a new homemade ISNAG fluorimeter with fluorescence measurements at ± 90◦ via 2×4 solar cell. The calibration graph was linear in the range of 1-45 mmol/L, with correlation coefficient r = 0.9762 and the limit of detection 29.785 µg/sample from the stepwise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph. The method was successfully applied to the determination of Ketotifen fumarate in two different pharmaceutical drugs. A comparison was made between the newly developed method analysis and the classical method using the standard addition method via the use of individual and paired t-test and F-test. It was noticed that there was no significant difference between the two methods at 95 % confidence level.

scholarly journals New Fluorometric Method for the Determination of Ketotifen Fumarate Using Continuous Flow Injection Analysis via ISNAG-fluorimeter

2019 ◽  
Vol 16 (2) ◽  
pp. 0353
Author(s):  
Turkie Et al.
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Classical Method ◽  
Calibration Graph ◽  
Injection System ◽  
Minimum Concentration ◽  
Ketotifen Fumarate ◽  
Fluorescence Measurements ◽  
Significant Difference

        A newly developed analytical method was conducted for the determination of Ketotifen fumarate (KTF) in pharmaceuticals drugs via quenching of continuous fluorescence of 9(10H)-Acridone (ACD). The method was applied using flow injection system of a new homemade ISNAG fluorimeter with fluorescence measurements at ± 90◦ via 2×4 solar cell. The calibration graph was linear in the range of 1-45 mmol/L, with correlation coefficient r = 0.9762 and the limit of detection 29.785 µg/sample from the stepwise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph. The method was successfully applied to the determination of Ketotifen fumarate in two different pharmaceutical drugs. A comparison was made between the newly developed method analysis and the classical method using the standard addition method via the use of individual and paired t-test and F-test. It was noticed that there was no significant difference between the two methods at 95 % confidence level.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations

2015 ◽  
Vol 50 ◽  
pp. 169-178
Author(s):  
Mouhammed Khateeb ◽  
Basheer Elias ◽  
Fatema Al Rahal
Keyword(s):  
Folic Acid ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Sulfuric Acid Medium ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A simple and sensitive kinetic spectrophotometric method has been developed for the determination of folic acid (FA) in bulk and pharmaceutical Formulations. The method is based on the oxidation of FA by Fe (III) in sulfuric acid medium. Fe (III) subsequently reduces to Fe (II) which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance at λmax 725 nm. The rate data and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1–20 μg mL-1 for each method. The correlation coefficient was 0.9978 and 0.9993, and LOD was found to be 0.91 and 0.09 μg mL-1 for rate data and fixed time methods, respectively. The proposed method has been successfully applied to the determination of FA in formulations with no interference from the excipients. Statical comparison of the results shows that there is no significant difference between the proposed and pharmacopoeial methods

scholarly journals Relationship between Vitamin B9 (Folic Acid), Vitamin B12 (Cobalamin), and Peripheral Neuropathy in Children with Beta-Thalassemia Major

2021 ◽  
Vol 9 (2) ◽  
Author(s):  
Uni Gamayani ◽  
Titin Junaidi ◽  
Nushrotul Lailiyya ◽  
Nur Suryawan ◽  
Nanan Sekarwana
Keyword(s):  
Folic Acid ◽  
Peripheral Neuropathy ◽  
Vitamin B12 ◽  
Thalassemia Major ◽  
Beta Thalassemia ◽  
Control Group ◽  
Case Group ◽  
Beta Thalassemia Major ◽  
Vitamin B9 ◽  
Significant Difference

Vitamin B9 (folic acid) and B12 (cobalamin) are essential vitamins that play roles in the process of hematopoiesis and maintaining the function of peripheral nerves. Therefore, these deficiencies may create a risk for peripheral neuropathy in beta-thalassemia major patients. The purpose of this study is to determine the relationship between vitamin B9 level, vitamin B12 level, and peripheral neuropathy in beta-thalassemia major children. It was an observational analytical study with a case-control design has been conducted at Dr. Hasan Sadikin General Hospital Bandung, Indonesia, in May–July 2019. There were 47 beta-thalassemia major children with peripheral neuropathy (case) and 41 healthy children (control). All subjects completed a general demographic questionnaire, underwent neurological examination, and were tested for vitamin B9 and B12 serum levels. Data were then analyzed using the unpaired t test to compare the vitamin levels between both groups and Spearman’s rank correlation test to investigate the correlation between vitamin levels and the number of affected nerves in the case group. Comparison of folic acid levels in the case group (21.52±6.22 ng/mL) and the control group (23.81±7.51 ng/mL) showed no significant difference (p=0.19). In contrast, cobalamin in the case group (288.57±168.61 ng/mL) and the control group (385.95±197.48 ng/mL) showed a significant difference (p=0.01). In addition, there was a moderate correlation (p=0.004, r=0.41) between folic acid level and the number of motoric nerves affected in the case group. In conclusion, cobalamin level correlates with peripheral neuropathy in beta-thalassemia major patients, and folic acid level correlates with the number of affected nerves, especially motoric nerves. HUBUNGAN ANTARA VITAMIN B9 (ASAM FOLAT), VITAMIN B12 (KOBALAMIN), DAN NEUROPATI PERIFER PADA ANAK DENGAN TALASEMIA BETA MAYORVitamin B9 (asam folat) dan B12 (kobalamin) merupakan vitamin esensial yang berperan dalam proses hematopoiesis dan menjaga fungsi saraf tepi. Defisiensi vitamin ini dapat menimbulkan risiko neuropati perifer pada pasien talasemia beta mayor. Tujuan penelitian ini mengetahui hubungan antara kadar vitamin B9, vitamin B12, dan neuropati perifer pada anak talasemia beta mayor. Metode penelitian ini adalah analitik observasional dengan rancangan studi kasus kontrol yang dilakukan di RSUP Dr. Hasan Sadikin Bandung, Indonesia pada Mei–Juli 2019. Terdapat 47 anak talasemia beta mayor dengan neuropati perifer (kelompok kasus) dan 41 anak sehat (kelompok kontrol). Seluruh subjek penelitian mengisi kuesioner demografi umum, menjalani pemeriksaan fisis neurologis, serta dilakukan tes kadar vitamin B9 dan B12 serum. Uji t test tidak berpasangan digunakan untuk membandingkan kadar vitamin pada kedua kelompok dan uji korelasi Spearman untuk membandingkan kadar kedua vitamin tersebut dengan jumlah saraf yang terkena pada kelompok kasus. Perbandingan kadar asam folat kelompok kasus (21,52±6,22 ng/mL) dan kelompok kontrol (23,81±7,51 ng/mL) menunjukkan perbedaan yang tidak bermakna (p=0,19), sedangkan perbandingan kadar kobalamin kelompok kasus (288,57±168,61 ng/mL) dan kelompok kontrol (385,95±197,48 ng/mL) menunjukkan perbedaan yang bermakna (p=0,01). Selain itu, terdapat korelasi sedang (p=0,004; r=0,41) antara kadar asam folat dam jumlah saraf motorik yang terkena pada kelompok kasus. Kesimpulan, kadar kobalamin berhubungan dengan neuropati perifer pada penderita talasemia beta mayor dan kadar asam folat berhubungan dengan jumlah saraf yang terkena, terutama saraf motorik.

scholarly journals New Method for the On-Line Determination of Molybdenum Via New Microphotometric Home Mode Instrument, Using Hight Emitting Diode as a Variable Intensity Radiant Source with a Miniture Photosilicone Diode Detector for System: Molybdenum(VI)-Hydrogen Peroxide-Ammonium Solution

2007 ◽  
Vol 4 (4) ◽  
pp. 571-582
Author(s):  
Baghdad Science Journal
Keyword(s):  
Hydrogen Peroxide ◽  
Classical Method ◽  
Wave Length ◽  
Research Work ◽  
Standard Addition ◽  
Ammonia Solution ◽  
Variable Intensity ◽  
Measurement Variation ◽  
On Line

This research work aims to the determination of molybdenum (VI) ion via the formation of peroxy molybdenum compounds which has red-brown colour with absorbance wave length at 455nm for the system of ammonia solution-hydrogen peroxide-molybdenum (VI) using a completely newly developed microphotometer based on the ON-Line measurement. Variation of responses expressed in millivolt. A correlation coefficient of 0.9925 for the range of 2.5-150 ?g.ml-1 with percentage linearity of 98.50%. A detection limit of 0.25 ?g.ml-1 was obtained. All physical and chemical variable were optimized interferences of cation and anion were studied classical method of measurement were done and compared well with newly on-line measurements. Application for the use of developed method on alloy samples, using standard addition method and agreed quite wall.

Determination of diltiazem based on the reduction of Cu(II)–BCA complexes in micellar medium

2010 ◽  
Vol 88 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Larissa Zuppardo Lacerda Sabino ◽  
Daniele Cestari Marino ◽  
Horacio Dorigan Moya
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Confidence Level ◽  
Limit Of Detection ◽  
Calibration Graph ◽  
Micellar Medium ◽  
Solution Ph ◽  
Simple Method ◽  
Relative Standard

A simple method was developed for determining microquantities of diltiazem, based on the reduction of copper(II) in buffered solution (pH 7.0) and the use of a micellar medium containing 4,4′-dicarboxy-2,2′-biquinoline acid. The copper(I) produced reacts with 4,4′-dicarboxy-2,2′-biquinoline acid and the complexes formed are spectrophotometrically measured at 558 nm. A typical calibration graph shows good linearity (r = 0.993) from 20 to 100 μg mL–1 of diltiazem. The limit of detection and relative standard deviation were calculated as 12 μg mL–1 (99% confidence level) and 3.5% (40 μg mL–1; n = 6), respectively, with a mean recovery value of 96.5% found in pharmaceutical dosages. A straightforward and effective way to recycle the reagents is addressed. The hazardous aspects of the Cu(I)–BCA reaction are presented as well.

scholarly journals Greener Analytical Method for Determination of Iodine Number of Edible Oils

2020 ◽  
Vol 9 (6) ◽  
pp. 36
Author(s):  
Thidarat Kruatian ◽  
Kritsana Jitmanee
Keyword(s):  
Iodine Number ◽  
Standard Method ◽  
Analytical Method ◽  
Present Method ◽  
Edible Oils ◽  
Classical Method ◽  
Sample Weight ◽  
Number Range ◽  
Significant Difference

A greener analytical method for determination of iodine number (IN) of oils is presented. As per the AOAC standard method, a large amount of solvent and reagent was used, and long incubation time was required. This research is aimed at using less amount of solvent and reagent, less sample weight, and shorten the analysis time by using the modified titrimetric AOAC standard method. The study showed that by reducing the sample size, the amount of reagent could be decreased to 1.00 mL and the reaction time of 1 min is enough for completion of the reaction. The amount of reagent used was at least 25 times less than that of the classical method. There was no significant difference at 95% confidence level between the results obtained by the proposed method and the standard method, and both results correlated well. The present method can be applied to edible oils commonly found in the market (iodine number range of 6.0 to 130).

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